##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
AatII Acc65I AccII AclI AcyI
AflIII AgeI AhdI Aor51HI ApaI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvaI AviII AvrII
BalI BamHI BbeI BbrPI BbvCI
BciVI BcnI BglI BglII BlnI
BsaBI BsaHI BsiEI BsiWI BsoBI
BspDI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstUI BstZ17I ClaI
CpoI DraIII DrdI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
EcoRI FauI FseI FspI HaeII
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MamI
MfeI MluI MluNI MscI MunI
MvnI NaeI NarI NciI NcoI
NdeI NgoMIV NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SphI
Sse8387I StuI SwaI TaqI Tth111I
TthHB8I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 33 enzymes) #
###################################
AciI 525 CCGC CCGC(-3/-1)
BanI 818 GGCACC G^G[CT][GA]CC
BbsI 961 GAAGAC GAAGAC(2/6)
BcgI 1145 CGACTGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 961 GAAGAC GAAGAC(2/6)
BsaAI 733 TACGTG [CT]AC^GT[GA]
BseRI 1711 GAGGAG GAGGAG(10/8)
BsgI 723 CTGCAC GTGCAG(16/14)
BsmBI 133 CGTCTC CGTCTC(1/5)
BsmI 1807 GAATGC GAATGC(1/-1)
BspHI 3225 TCATGA T^CATGA
BspMI 2366 GCAGGT ACCTGC(4/8)
BsrFI 976 ACCGGC [GA]^CCGG[CT]
BstAPI 2524 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1397 GGTGACC G^GT[ACGT]ACC
BstPI 1397 GGTGACC G^GT[ACGT]ACC
Cfr10I 976 ACCGGC [GA]^CCGG[CT]
DraI 3019 TTTAAA TTT^AAA
DsaI 870 CCGTGG C^C[GA][CT]GG
EcoO65I 1397 GGTGACC G^GT[ACGT]ACC
EcoRV 338 GATATC GAT^ATC
EcoT22I 2992 ATGCAT ATGCA^T
HgaI 997 GACGC GACGC(5/10)
MaeII 734 ACGT A^CGT
NheI 3098 GCTAGC G^CTAGC
NsiI 2992 ATGCAT ATGCA^T
PflMI 384 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 3156 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 3225 TCATGA T^CATGA
SpeI 1422 ACTAGT A^CTAGT
SspI 2914 AATATT AAT^ATT
Van91I 384 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 877 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 242 GTAGAC GT^[AC][GT]AC
3124 GTCTAC
AccIII 121 TCCGGA T^CCGGA
291 TCCGGA
Alw44I 1153 GTGCAC G^TGCAC
3271 GTGCAC
AlwI 1452 GATCC GGATC(4/5)
2549 GATCC
ApaLI 1153 GTGCAC G^TGCAC
3271 GTGCAC
BsaI 2101 GAGACC GGTCTC(1/5)
2177 GAGACC
BsaWI 121 TCCGGA [AT]^CCGG[AT]
291 TCCGGA
BseAI 121 TCCGGA T^CCGGA
291 TCCGGA
BspEI 121 TCCGGA T^CCGGA
291 TCCGGA
BsrDI 806 CATTGC GCAATG(2/0)
1831 GCAATG
BstXI 1981 CCAAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2246 CCAGGTAACTGG
BstYI 1451 AGATCC [GA]^GATC[CT]
2548 AGATCC
CfoI 770 GCGC GCG^C
1507 GCGC
EcoNI 411 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1047 CCTGTGATAGG
HhaI 770 GCGC GCG^C
1507 GCGC
HinP1I 770 GCGC G^CGC
1507 GCGC
MflI 1451 AGATCC [GA]^GATC[CT]
2548 AGATCC
MroI 121 TCCGGA T^CCGGA
291 TCCGGA
NspI 738 GCATGT [GA]CATG^[CT]
2990 ACATGC
SacI 553 GAGCTC GAGCT^C
1483 GAGCTC
XhoII 1451 AGATCC [GA]^GATC[CT]
2548 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
#########################################
AcsI AfaI AflII AluI AlwNI
AocI ApoI AspHI AvaII BanII
BbvI BclI BfaI BfrI BlpI
BmyI BpmI Bpu1102I BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BsrGI BsrI BssKI BstF5I
BstNI Bsu36I Cac8I CelII Cfr13I
DdeI DpnII DraII Eco81I EcoO109I
EcoRII EcoT14I FbaI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI MaeI MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI