################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## AatII Acc65I AccII AclI AcyI AflIII AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvaI AviII AvrII BalI BamHI BbeI BbrPI BbvCI BciVI BcnI BglI BglII BlnI BsaBI BsaHI BsiEI BsiWI BsoBI BspDI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstUI BstZ17I ClaI CpoI DraIII DrdI EaeI EagI Eam1105I EarI EclXI Eco47III Eco52I EcoRI FauI FseI FspI HaeII Hin1I HincII HindII HindIII HpaI KasI KpnI Ksp632I KspI MamI MfeI MluI MluNI MscI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SphI Sse8387I StuI SwaI TaqI Tth111I TthHB8I XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 33 enzymes) # ################################### AciI 525 CCGC CCGC(-3/-1) BanI 818 GGCACC G^G[CT][GA]CC BbsI 961 GAAGAC GAAGAC(2/6) BcgI 1145 CGACTGATGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 961 GAAGAC GAAGAC(2/6) BsaAI 733 TACGTG [CT]AC^GT[GA] BseRI 1711 GAGGAG GAGGAG(10/8) BsgI 723 CTGCAC GTGCAG(16/14) BsmBI 133 CGTCTC CGTCTC(1/5) BsmI 1807 GAATGC GAATGC(1/-1) BspHI 3225 TCATGA T^CATGA BspMI 2366 GCAGGT ACCTGC(4/8) BsrFI 976 ACCGGC [GA]^CCGG[CT] BstAPI 2524 GCAAATTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1397 GGTGACC G^GT[ACGT]ACC BstPI 1397 GGTGACC G^GT[ACGT]ACC Cfr10I 976 ACCGGC [GA]^CCGG[CT] DraI 3019 TTTAAA TTT^AAA DsaI 870 CCGTGG C^C[GA][CT]GG EcoO65I 1397 GGTGACC G^GT[ACGT]ACC EcoRV 338 GATATC GAT^ATC EcoT22I 2992 ATGCAT ATGCA^T HgaI 997 GACGC GACGC(5/10) MaeII 734 ACGT A^CGT NheI 3098 GCTAGC G^CTAGC NsiI 2992 ATGCAT ATGCA^T PflMI 384 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 3156 GGGTCCT [GA]G^G[AT]CC[CT] RcaI 3225 TCATGA T^CATGA SpeI 1422 ACTAGT A^CTAGT SspI 2914 AATATT AAT^ATT Van91I 384 CCAAAGACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 877 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 242 GTAGAC GT^[AC][GT]AC 3124 GTCTAC AccIII 121 TCCGGA T^CCGGA 291 TCCGGA Alw44I 1153 GTGCAC G^TGCAC 3271 GTGCAC AlwI 1452 GATCC GGATC(4/5) 2549 GATCC ApaLI 1153 GTGCAC G^TGCAC 3271 GTGCAC BsaI 2101 GAGACC GGTCTC(1/5) 2177 GAGACC BsaWI 121 TCCGGA [AT]^CCGG[AT] 291 TCCGGA BseAI 121 TCCGGA T^CCGGA 291 TCCGGA BspEI 121 TCCGGA T^CCGGA 291 TCCGGA BsrDI 806 CATTGC GCAATG(2/0) 1831 GCAATG BstXI 1981 CCAAGTATTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2246 CCAGGTAACTGG BstYI 1451 AGATCC [GA]^GATC[CT] 2548 AGATCC CfoI 770 GCGC GCG^C 1507 GCGC EcoNI 411 CCTACAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1047 CCTGTGATAGG HhaI 770 GCGC GCG^C 1507 GCGC HinP1I 770 GCGC G^CGC 1507 GCGC MflI 1451 AGATCC [GA]^GATC[CT] 2548 AGATCC MroI 121 TCCGGA T^CCGGA 291 TCCGGA NspI 738 GCATGT [GA]CATG^[CT] 2990 ACATGC SacI 553 GAGCTC GAGCT^C 1483 GAGCTC XhoII 1451 AGATCC [GA]^GATC[CT] 2548 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 83 enzymes) # ######################################### AcsI AfaI AflII AluI AlwNI AocI ApoI AspHI AvaII BanII BbvI BclI BfaI BfrI BlpI BmyI BpmI Bpu1102I BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrGI BsrI BssKI BstF5I BstNI Bsu36I Cac8I CelII Cfr13I DdeI DpnII DraII Eco81I EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI