##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII AccIII AclI AcyI AgeI
Alw44I Aor51HI ApaLI AscI AseI
AsnI AspI AviII BbeI BbrPI
BbsI BclI BpuAI BsaAI BsaBI
BsaHI BseAI BsiEI BsiWI BspDI
BspEI BssHII Bst1107I BstAPI BstEII
BstPI BstXI BstZ17I ClaI CpoI
DraI DraIII DrdI EagI EclXI
Eco47III Eco52I EcoO65I EcoRV FbaI
FseI FspI Hin1I HindIII KasI
KspI MamI MfeI MluI MroI
MunI NarI NcoI NdeI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SspI
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 277 GGTACC G^GTACC
AccI 714 GTCTAC GT^[AC][GT]AC
AcsI 2024 GAATTC [GA]^AATT[CT]
AflII 2823 CTTAAG C^TTAAG
AhdI 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2024 GAATTC [GA]^AATT[CT]
Asp718I 277 GGTACC G^GTACC
AspEI 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1286 CCTAGG C^CTAGG
BbvCI 560 CCTCAGC CCTCAGC(-5/-2)
BciVI 610 GTATCC GTATCC(6/5)
BfrI 2823 CTTAAG C^TTAAG
BglI 2558 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2301 AGATCT A^GATCT
BlnI 1286 CCTAGG C^CTAGG
BlpI 824 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 824 GCTGAGC GC^T[ACGT]AGC
BsaWI 2545 ACCGGA [AT]^CCGG[AT]
BsmBI 1424 CGTCTC CGTCTC(1/5)
Bsp1407I 256 TGTACA T^GTACA
BspHI 1490 TCATGA T^CATGA
BspLU11I 2791 ACATGT A^CATGT
BsrBI 2638 CCGCTC CCGCTC(-3/-3)
BsrFI 565 GCCGGC [GA]^CCGG[CT]
BsrGI 256 TGTACA T^GTACA
BstBI 801 TTCGAA TT^CGAA
CelII 824 GCTGAGC GC^T[ACGT]AGC
Cfr10I 565 GCCGGC [GA]^CCGG[CT]
DsaI 232 CCGTGG C^C[GA][CT]GG
Eam1105I 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2024 GAATTC G^AATTC
HgaI 67 GCGTC GACGC(5/10)
HincII 498 GTTAAC GT[CT]^[GA]AC
HindII 498 GTTAAC GT[CT]^[GA]AC
HpaI 498 GTTAAC GTT^AAC
KpnI 277 GGTACC GGTAC^C
NaeI 565 GCCGGC GCC^GGC
NgoMIV 565 GCCGGC G^CCGGC
NheI 239 GCTAGC G^CTAGC
NspV 801 TTCGAA TT^CGAA
PstI 132 CTGCAG CTGCA^G
PvuII 2622 CAGCTG CAG^CTG
RcaI 1490 TCATGA T^CATGA
SacI 827 GAGCTC GAGCT^C
SapI 773 GCTCTTC GCTCTTC(1/4)
SfuI 801 TTCGAA TT^CGAA
SpeI 2550 ACTAGT A^CTAGT
SspBI 256 TGTACA T^GTACA
StuI 2267 AGGCCT AGG^CCT
Tsp509I 2025 AATT ^AATT
XbaI 2808 TCTAGA T^CTAGA
XcmI 223 CCATGAAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccII 66 CGCG CG^CG
1020 CGCG
AflIII 1639 ACGTGT A^C[GA][CT]GT
2791 ACATGT
Asp700I 192 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2628 GAATGCCTTC
BamHI 79 GGATCC G^GATCC
2180 GGATCC
BcgI 463 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2352 GCACTATGGTCG
BsmI 153 GAATGC GAATGC(1/-1)
2628 GAATGC
BssSI 940 CTCGTG CACGAG(-5/-1)
2756 CTCGTG
BstUI 66 CGCG CG^CG
1020 CGCG
EcoT22I 1152 ATGCAT ATGCA^T
2846 ATGCAT
HaeII 35 GGCGCT [GA]GCGC^[CT]
2893 GGCGCT
MaeIII 556 GTGAC ^GT[ACGT]AC
1689 GTGAC
MslI 148 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
639 CATCAAGATG
MspA1I 811 CAGCGG C[AC]G^C[GT]G
2622 CAGCTG
MvnI 66 CGCG CG^CG
1020 CGCG
NsiI 1152 ATGCAT ATGCA^T
2846 ATGCAT
SmaI 1111 CCCGGG CCC^GGG
1932 CCCGGG
SmlI 700 CTCAAG C^T[CT][GA]AG
2823 CTTAAG
Tsp45I 556 GTGAC ^GT[GC]AC
1689 GTGAC
XmaCI 1111 CCCGGG C^CCGGG
1932 CCCGGG
XmaI 1111 CCCGGG C^CCGGG
1932 CCCGGG
XmnI 192 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2628 GAATGCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrDI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MboI
MboII MflI MluNI MnlI MscI
MseI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI TspRI TthHB8I
Van91I XhoII