##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AatII       AccIII      AclI        AcyI        AgeI        
Alw44I      Aor51HI     ApaLI       AscI        AseI        
AsnI        AspI        AviII       BbeI        BbrPI       
BbsI        BclI        BpuAI       BsaAI       BsaBI       
BsaHI       BseAI       BsiEI       BsiWI       BspDI       
BspEI       BssHII      Bst1107I    BstAPI      BstEII      
BstPI       BstXI       BstZ17I     ClaI        CpoI        
DraI        DraIII      DrdI        EagI        EclXI       
Eco47III    Eco52I      EcoO65I     EcoRV       FbaI        
FseI        FspI        Hin1I       HindIII     KasI        
KspI        MamI        MfeI        MluI        MroI        
MunI        NarI        NcoI        NdeI        NotI        
NruI        PacI        PaeR7I      PflFI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RsrII       SacII       SalI        
SbfI        ScaI        SexAI       SfiI        SfoI        
SgrAI       SnaBI       SphI        Sse8387I    SspI        
SwaI        Tth111I     XhoI        


###################################
# ONE CUT ENZYMES (   52 enzymes) #
###################################

Acc65I        277  GGTACC               G^GTACC
AccI          714  GTCTAC               GT^[AC][GT]AC
AcsI         2024  GAATTC               [GA]^AATT[CT]
AflII        2823  CTTAAG               C^TTAAG
AhdI         1697  GACTCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         2024  GAATTC               [GA]^AATT[CT]
Asp718I       277  GGTACC               G^GTACC
AspEI        1697  GACTCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII        1286  CCTAGG               C^CTAGG
BbvCI         560  CCTCAGC              CCTCAGC(-5/-2)
BciVI         610  GTATCC               GTATCC(6/5)
BfrI         2823  CTTAAG               C^TTAAG
BglI         2558  GCCTTCCCGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2301  AGATCT               A^GATCT
BlnI         1286  CCTAGG               C^CTAGG
BlpI          824  GCTGAGC              GC^T[ACGT]AGC
Bpu1102I      824  GCTGAGC              GC^T[ACGT]AGC
BsaWI        2545  ACCGGA               [AT]^CCGG[AT]
BsmBI        1424  CGTCTC               CGTCTC(1/5)
Bsp1407I      256  TGTACA               T^GTACA
BspHI        1490  TCATGA               T^CATGA
BspLU11I     2791  ACATGT               A^CATGT
BsrBI        2638  CCGCTC               CCGCTC(-3/-3)
BsrFI         565  GCCGGC               [GA]^CCGG[CT]
BsrGI         256  TGTACA               T^GTACA
BstBI         801  TTCGAA               TT^CGAA
CelII         824  GCTGAGC              GC^T[ACGT]AGC
Cfr10I        565  GCCGGC               [GA]^CCGG[CT]
DsaI          232  CCGTGG               C^C[GA][CT]GG
Eam1105I     1697  GACTCTAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI        2024  GAATTC               G^AATTC
HgaI           67  GCGTC                GACGC(5/10)
HincII        498  GTTAAC               GT[CT]^[GA]AC
HindII        498  GTTAAC               GT[CT]^[GA]AC
HpaI          498  GTTAAC               GTT^AAC
KpnI          277  GGTACC               GGTAC^C
NaeI          565  GCCGGC               GCC^GGC
NgoMIV        565  GCCGGC               G^CCGGC
NheI          239  GCTAGC               G^CTAGC
NspV          801  TTCGAA               TT^CGAA
PstI          132  CTGCAG               CTGCA^G
PvuII        2622  CAGCTG               CAG^CTG
RcaI         1490  TCATGA               T^CATGA
SacI          827  GAGCTC               GAGCT^C
SapI          773  GCTCTTC              GCTCTTC(1/4)
SfuI          801  TTCGAA               TT^CGAA
SpeI         2550  ACTAGT               A^CTAGT
SspBI         256  TGTACA               T^GTACA
StuI         2267  AGGCCT               AGG^CCT
Tsp509I      2025  AATT                 ^AATT
XbaI         2808  TCTAGA               T^CTAGA
XcmI          223  CCATGAAAGCCGTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   21 enzymes) #
###################################

AccII          66  CGCG                 CG^CG
             1020  CGCG                
AflIII       1639  ACGTGT               A^C[GA][CT]GT
             2791  ACATGT              
Asp700I       192  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2628  GAATGCCTTC          
BamHI          79  GGATCC               G^GATCC
             2180  GGATCC              
BcgI          463  CGAGGGCTGTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
             2352  GCACTATGGTCG        
BsmI          153  GAATGC               GAATGC(1/-1)
             2628  GAATGC              
BssSI         940  CTCGTG               CACGAG(-5/-1)
             2756  CTCGTG              
BstUI          66  CGCG                 CG^CG
             1020  CGCG                
EcoT22I      1152  ATGCAT               ATGCA^T
             2846  ATGCAT              
HaeII          35  GGCGCT               [GA]GCGC^[CT]
             2893  GGCGCT              
MaeIII        556  GTGAC                ^GT[ACGT]AC
             1689  GTGAC               
MslI          148  CACCAGAATG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
              639  CATCAAGATG          
MspA1I        811  CAGCGG               C[AC]G^C[GT]G
             2622  CAGCTG              
MvnI           66  CGCG                 CG^CG
             1020  CGCG                
NsiI         1152  ATGCAT               ATGCA^T
             2846  ATGCAT              
SmaI         1111  CCCGGG               CCC^GGG
             1932  CCCGGG              
SmlI          700  CTCAAG               C^T[CT][GA]AG
             2823  CTTAAG              
Tsp45I        556  GTGAC                ^GT[GC]AC
             1689  GTGAC               
XmaCI        1111  CCCGGG               C^CCGGG
             1932  CCCGGG              
XmaI         1111  CCCGGG               C^CCGGG
             1932  CCCGGG              
XmnI          192  GAATACATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             2628  GAATGCCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   97 enzymes) #
#########################################

AciI        AfaI        AluI        AlwI        AlwNI       
AocI        ApaI        AspHI       AvaI        AvaII       
BalI        BanI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaI        BsaJI       
BseRI       BsgI        BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BspMI       
BsrDI       BsrI        BssKI       BstF5I      BstNI       
BstYI       Bsu36I      Cac8I       CfoI        Cfr13I      
DdeI        DpnII       DraII       EaeI        EarI        
Eco81I      EcoNI       EcoO109I    EcoRII      EcoT14I     
FauI        Fnu4HI      FokI        HaeIII      HapII       
HhaI        HinP1I      HinfI       HpaII       HphI        
ItaI        Ksp632I     MaeI        MaeII       MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MspI        MvaI        MwoI        NciI        
NdeII       NlaIII      NlaIV       NspI        PflMI       
PleI        PpuMI       RsaI        Sau3AI      Sau96I      
ScrFI       SfaNI       SfcI        StyI        TaqI        
TfiI        Tru9I       TseI        TspRI       TthHB8I     
Van91I      XhoII