################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII AccIII AclI AcyI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI AspI AviII BbeI BbrPI BbsI BclI BpuAI BsaAI BsaBI BsaHI BseAI BsiEI BsiWI BspDI BspEI BssHII Bst1107I BstAPI BstEII BstPI BstXI BstZ17I ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I EcoO65I EcoRV FbaI FseI FspI Hin1I HindIII KasI KspI MamI MfeI MluI MroI MunI NarI NcoI NdeI NotI NruI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SnaBI SphI Sse8387I SspI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### Acc65I 277 GGTACC G^GTACC AccI 714 GTCTAC GT^[AC][GT]AC AcsI 2024 GAATTC [GA]^AATT[CT] AflII 2823 CTTAAG C^TTAAG AhdI 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 2024 GAATTC [GA]^AATT[CT] Asp718I 277 GGTACC G^GTACC AspEI 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 1286 CCTAGG C^CTAGG BbvCI 560 CCTCAGC CCTCAGC(-5/-2) BciVI 610 GTATCC GTATCC(6/5) BfrI 2823 CTTAAG C^TTAAG BglI 2558 GCCTTCCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2301 AGATCT A^GATCT BlnI 1286 CCTAGG C^CTAGG BlpI 824 GCTGAGC GC^T[ACGT]AGC Bpu1102I 824 GCTGAGC GC^T[ACGT]AGC BsaWI 2545 ACCGGA [AT]^CCGG[AT] BsmBI 1424 CGTCTC CGTCTC(1/5) Bsp1407I 256 TGTACA T^GTACA BspHI 1490 TCATGA T^CATGA BspLU11I 2791 ACATGT A^CATGT BsrBI 2638 CCGCTC CCGCTC(-3/-3) BsrFI 565 GCCGGC [GA]^CCGG[CT] BsrGI 256 TGTACA T^GTACA BstBI 801 TTCGAA TT^CGAA CelII 824 GCTGAGC GC^T[ACGT]AGC Cfr10I 565 GCCGGC [GA]^CCGG[CT] DsaI 232 CCGTGG C^C[GA][CT]GG Eam1105I 1697 GACTCTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2024 GAATTC G^AATTC HgaI 67 GCGTC GACGC(5/10) HincII 498 GTTAAC GT[CT]^[GA]AC HindII 498 GTTAAC GT[CT]^[GA]AC HpaI 498 GTTAAC GTT^AAC KpnI 277 GGTACC GGTAC^C NaeI 565 GCCGGC GCC^GGC NgoMIV 565 GCCGGC G^CCGGC NheI 239 GCTAGC G^CTAGC NspV 801 TTCGAA TT^CGAA PstI 132 CTGCAG CTGCA^G PvuII 2622 CAGCTG CAG^CTG RcaI 1490 TCATGA T^CATGA SacI 827 GAGCTC GAGCT^C SapI 773 GCTCTTC GCTCTTC(1/4) SfuI 801 TTCGAA TT^CGAA SpeI 2550 ACTAGT A^CTAGT SspBI 256 TGTACA T^GTACA StuI 2267 AGGCCT AGG^CCT Tsp509I 2025 AATT ^AATT XbaI 2808 TCTAGA T^CTAGA XcmI 223 CCATGAAAGCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccII 66 CGCG CG^CG 1020 CGCG AflIII 1639 ACGTGT A^C[GA][CT]GT 2791 ACATGT Asp700I 192 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2628 GAATGCCTTC BamHI 79 GGATCC G^GATCC 2180 GGATCC BcgI 463 CGAGGGCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 2352 GCACTATGGTCG BsmI 153 GAATGC GAATGC(1/-1) 2628 GAATGC BssSI 940 CTCGTG CACGAG(-5/-1) 2756 CTCGTG BstUI 66 CGCG CG^CG 1020 CGCG EcoT22I 1152 ATGCAT ATGCA^T 2846 ATGCAT HaeII 35 GGCGCT [GA]GCGC^[CT] 2893 GGCGCT MaeIII 556 GTGAC ^GT[ACGT]AC 1689 GTGAC MslI 148 CACCAGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 639 CATCAAGATG MspA1I 811 CAGCGG C[AC]G^C[GT]G 2622 CAGCTG MvnI 66 CGCG CG^CG 1020 CGCG NsiI 1152 ATGCAT ATGCA^T 2846 ATGCAT SmaI 1111 CCCGGG CCC^GGG 1932 CCCGGG SmlI 700 CTCAAG C^T[CT][GA]AG 2823 CTTAAG Tsp45I 556 GTGAC ^GT[GC]AC 1689 GTGAC XmaCI 1111 CCCGGG C^CCGGG 1932 CCCGGG XmaI 1111 CCCGGG C^CCGGG 1932 CCCGGG XmnI 192 GAATACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2628 GAATGCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI Eco81I EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MboI MboII MflI MluNI MnlI MscI MseI MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI TspRI TthHB8I Van91I XhoII