################################## # NO CUT ENZYMES ( 97 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflIII AgeI AhdI AscI Asp718I AspEI AspI AvaI AviII BamHI BanI BbeI BbrPI BcgI BcnI BlpI Bpu1102I BsaAI BsaHI BseAI BsiEI BsiWI BsmBI BsoBI BspDI BspEI BspLU11I BssHII Bst1107I BstAPI BstBI BstEII BstPI BstZ17I CelII ClaI DraIII DrdI EagI Eam1105I EclXI Eco52I EcoO65I EcoRV FseI FspI Hin1I KasI KpnI MaeII MfeI MluI MroI MunI NarI NciI NcoI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I StuI SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### Alw44I 2063 GTGCAC G^TGCAC AlwNI 67 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG AocI 1291 CCTCAGG CC^T[ACGT]AGG ApaI 162 GGGCCC GGGCC^C ApaLI 2063 GTGCAC G^TGCAC Asp700I 2114 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvrII 2802 CCTAGG C^CTAGG BalI 63 TGGCCA TGG^CCA BanII 162 GGGCCC G[GA]GC[CT]^C BbvCI 1145 CCTCAGC CCTCAGC(-5/-2) BciVI 1420 GTATCC GTATCC(6/5) BclI 3066 TGATCA T^GATCA BlnI 2802 CCTAGG C^CTAGG BsaI 1971 GAGACC GGTCTC(1/5) BsaWI 1134 TCCGGT [AT]^CCGG[AT] BsrBI 27 GAGCGG CCGCTC(-3/-3) BsrDI 1407 CATTGC GCAATG(2/0) BsrFI 17 GCCGGC [GA]^CCGG[CT] BssSI 151 CACGAG CACGAG(-5/-1) BstXI 1676 CCAACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1291 CCTCAGG CC^T[ACGT]AGG Cfr10I 17 GCCGGC [GA]^CCGG[CT] CpoI 77 CGGACCG CG^G[AT]CCG EaeI 63 TGGCCA [CT]^GGCC[GA] Eco81I 1291 CCTCAGG CC^T[ACGT]AGG FbaI 3066 TGATCA T^GATCA HgaI 1080 GCGTC GACGC(5/10) HindIII 1359 AAGCTT A^AGCTT MluNI 63 TGGCCA TGG^CCA MscI 63 TGGCCA TGG^CCA NaeI 17 GCCGGC GCC^GGC NgoMIV 17 GCCGGC G^CCGGC PpuMI 3 GGGTCCC [GA]G^G[AT]CC[CT] RsrII 77 CGGACCG CG^G[AT]CCG SexAI 1740 ACCAGGT A^CC[AT]GGT SpeI 2172 ACTAGT A^CTAGT SphI 1826 GCATGC GCATG^C TaqI 485 TCGA T^CGA TthHB8I 485 TCGA T^CGA XbaI 1332 TCTAGA T^CTAGA XcmI 1792 CCAACAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2114 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 417 GTCTAC GT^[AC][GT]AC 2525 GTAGAC Aor51HI 1807 AGCGCT AGC^GCT 2133 AGCGCT AvaII 4 GGTCC G^G[AT]CC 78 GGACC BglI 12 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1276 GCCATGTCGGC BglII 852 AGATCT A^GATCT 2238 AGATCT BsaBI 2408 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2635 GATTTTTATC BsgI 1166 CTGCAC GTGCAG(16/14) 3148 CTGCAC BsmFI 2169 GGGAC GGGAC(10/14) 5 GTCCC BsmI 2666 GAATGC GAATGC(1/-1) 481 GCATTC Bsp1407I 199 TGTACA T^GTACA 3241 TGTACA BspHI 1449 TCATGA T^CATGA 1967 TCATGA BspMI 1232 ACCTGC ACCTGC(4/8) 1400 ACCTGC BsrGI 199 TGTACA T^GTACA 3241 TGTACA DsaI 89 CCGCGG C^C[GA][CT]GG 116 CCGCGG Eco47III 1807 AGCGCT AGC^GCT 2133 AGCGCT EcoNI 1993 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3205 CCTTAAGAAGG EcoRI 1901 GAATTC G^AATTC 2735 GAATTC EcoT14I 908 CCTTGG C^C[AT][AT]GG 2802 CCTAGG FauI 14 CCCGC CCCGC(4/6) 2162 GCGGG HaeII 1807 AGCGCT [GA]GCGC^[CT] 2133 AGCGCT HapII 18 CCGG C^CGG 1135 CCGG HpaI 1755 GTTAAC GTT^AAC 2484 GTTAAC HpaII 18 CCGG C^CGG 1135 CCGG KspI 89 CCGCGG CCGC^GG 116 CCGCGG MamI 2408 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2635 GATTTTTATC MslI 492 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2645 CACAAGAATG MspI 18 CCGG C^CGG 1135 CCGG NspI 1826 GCATGC [GA]CATG^[CT] 2902 GCATGT PleI 1611 GAGTC GAGTC(4/5) 1734 GAGTC PstI 1124 CTGCAG CTGCA^G 3275 CTGCAG RcaI 1449 TCATGA T^CATGA 1967 TCATGA SacII 89 CCGCGG CCGC^GG 116 CCGCGG SspBI 199 TGTACA T^GTACA 3241 TGTACA StyI 908 CCTTGG C^C[AT][AT]GG 2802 CCTAGG Tsp45I 106 GTCAC ^GT[GC]AC 2253 GTGAC ######################################### # 3 OR MORE CUT ENZYMES ( 84 enzymes) # ######################################### AccII AciI AcsI AfaI AflII AluI AlwI ApoI AseI AsnI AspHI BbsI BbvI BfaI BfrI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII EarI EcoO109I EcoRII EcoT22I Fnu4HI FokI HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MvnI MwoI NdeII NlaIII NlaIV NsiI PshBI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI TfiI Tru9I TseI Tsp509I TspRI XhoII