##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflIII AgeI AhdI AscI Asp718I
AspEI AspI AvaI AviII BamHI
BanI BbeI BbrPI BcgI BcnI
BlpI Bpu1102I BsaAI BsaHI BseAI
BsiEI BsiWI BsmBI BsoBI BspDI
BspEI BspLU11I BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I CelII
ClaI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI Hin1I KasI KpnI MaeII
MfeI MluI MroI MunI NarI
NciI NcoI NdeI NheI NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I PvuI SacI SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I Van91I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Alw44I 2063 GTGCAC G^TGCAC
AlwNI 67 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1291 CCTCAGG CC^T[ACGT]AGG
ApaI 162 GGGCCC GGGCC^C
ApaLI 2063 GTGCAC G^TGCAC
Asp700I 2114 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 2802 CCTAGG C^CTAGG
BalI 63 TGGCCA TGG^CCA
BanII 162 GGGCCC G[GA]GC[CT]^C
BbvCI 1145 CCTCAGC CCTCAGC(-5/-2)
BciVI 1420 GTATCC GTATCC(6/5)
BclI 3066 TGATCA T^GATCA
BlnI 2802 CCTAGG C^CTAGG
BsaI 1971 GAGACC GGTCTC(1/5)
BsaWI 1134 TCCGGT [AT]^CCGG[AT]
BsrBI 27 GAGCGG CCGCTC(-3/-3)
BsrDI 1407 CATTGC GCAATG(2/0)
BsrFI 17 GCCGGC [GA]^CCGG[CT]
BssSI 151 CACGAG CACGAG(-5/-1)
BstXI 1676 CCAACCTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1291 CCTCAGG CC^T[ACGT]AGG
Cfr10I 17 GCCGGC [GA]^CCGG[CT]
CpoI 77 CGGACCG CG^G[AT]CCG
EaeI 63 TGGCCA [CT]^GGCC[GA]
Eco81I 1291 CCTCAGG CC^T[ACGT]AGG
FbaI 3066 TGATCA T^GATCA
HgaI 1080 GCGTC GACGC(5/10)
HindIII 1359 AAGCTT A^AGCTT
MluNI 63 TGGCCA TGG^CCA
MscI 63 TGGCCA TGG^CCA
NaeI 17 GCCGGC GCC^GGC
NgoMIV 17 GCCGGC G^CCGGC
PpuMI 3 GGGTCCC [GA]G^G[AT]CC[CT]
RsrII 77 CGGACCG CG^G[AT]CCG
SexAI 1740 ACCAGGT A^CC[AT]GGT
SpeI 2172 ACTAGT A^CTAGT
SphI 1826 GCATGC GCATG^C
TaqI 485 TCGA T^CGA
TthHB8I 485 TCGA T^CGA
XbaI 1332 TCTAGA T^CTAGA
XcmI 1792 CCAACAACTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2114 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 417 GTCTAC GT^[AC][GT]AC
2525 GTAGAC
Aor51HI 1807 AGCGCT AGC^GCT
2133 AGCGCT
AvaII 4 GGTCC G^G[AT]CC
78 GGACC
BglI 12 GCCCCGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1276 GCCATGTCGGC
BglII 852 AGATCT A^GATCT
2238 AGATCT
BsaBI 2408 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2635 GATTTTTATC
BsgI 1166 CTGCAC GTGCAG(16/14)
3148 CTGCAC
BsmFI 2169 GGGAC GGGAC(10/14)
5 GTCCC
BsmI 2666 GAATGC GAATGC(1/-1)
481 GCATTC
Bsp1407I 199 TGTACA T^GTACA
3241 TGTACA
BspHI 1449 TCATGA T^CATGA
1967 TCATGA
BspMI 1232 ACCTGC ACCTGC(4/8)
1400 ACCTGC
BsrGI 199 TGTACA T^GTACA
3241 TGTACA
DsaI 89 CCGCGG C^C[GA][CT]GG
116 CCGCGG
Eco47III 1807 AGCGCT AGC^GCT
2133 AGCGCT
EcoNI 1993 CCTGTATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3205 CCTTAAGAAGG
EcoRI 1901 GAATTC G^AATTC
2735 GAATTC
EcoT14I 908 CCTTGG C^C[AT][AT]GG
2802 CCTAGG
FauI 14 CCCGC CCCGC(4/6)
2162 GCGGG
HaeII 1807 AGCGCT [GA]GCGC^[CT]
2133 AGCGCT
HapII 18 CCGG C^CGG
1135 CCGG
HpaI 1755 GTTAAC GTT^AAC
2484 GTTAAC
HpaII 18 CCGG C^CGG
1135 CCGG
KspI 89 CCGCGG CCGC^GG
116 CCGCGG
MamI 2408 GATCAAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2635 GATTTTTATC
MslI 492 CACAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2645 CACAAGAATG
MspI 18 CCGG C^CGG
1135 CCGG
NspI 1826 GCATGC [GA]CATG^[CT]
2902 GCATGT
PleI 1611 GAGTC GAGTC(4/5)
1734 GAGTC
PstI 1124 CTGCAG CTGCA^G
3275 CTGCAG
RcaI 1449 TCATGA T^CATGA
1967 TCATGA
SacII 89 CCGCGG CCGC^GG
116 CCGCGG
SspBI 199 TGTACA T^GTACA
3241 TGTACA
StyI 908 CCTTGG C^C[AT][AT]GG
2802 CCTAGG
Tsp45I 106 GTCAC ^GT[GC]AC
2253 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AccII AciI AcsI AfaI AflII
AluI AlwI ApoI AseI AsnI
AspHI BbsI BbvI BfaI BfrI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII EarI EcoO109I
EcoRII EcoT22I Fnu4HI FokI HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MspA1I MvaI MvnI MwoI NdeII
NlaIII NlaIV NsiI PshBI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspI TfiI Tru9I
TseI Tsp509I TspRI XhoII