################################## # NO CUT ENZYMES ( 39 enzymes) # ################################## AatII AgeI AscI BcgI BciVI BsaBI BsiWI BspDI BssHII Bst1107I BstZ17I ClaI CpoI DraI EcoRI EcoT22I HpaI KspI MamI MfeI MluI MunI NdeI NotI NruI NsiI PacI PinAI PmeI PvuI RsrII SacII SbfI SgrAI SnaBI SpeI Sse8387I SspI SwaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 6033 GGTACC G^GTACC AccII 3517 CGCG CG^CG AccIII 382 TCCGGA T^CCGGA AclI 5411 AACGTT AA^CGTT AflIII 4270 ACATGT A^C[GA][CT]GT AhdI 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 7987 ATTAAT AT^TAAT AsnI 7987 ATTAAT AT^TAAT Asp718I 6033 GGTACC G^GTACC AspEI 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 217 GGATCC G^GATCC BclI 6352 TGATCA T^GATCA BseAI 382 TCCGGA T^CCGGA BsmI 6218 GAATGC GAATGC(1/-1) BspEI 382 TCCGGA T^CCGGA BspHI 448 TCATGA T^CATGA BspLU11I 4270 ACATGT A^CATGT BssSI 769 CACGAG CACGAG(-5/-1) BstBI 7551 TTCGAA TT^CGAA BstUI 3517 CGCG CG^CG EagI 3926 CGGCCG C^GGCCG Eam1105I 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 3926 CGGCCG C^GGCCG Eco52I 3926 CGGCCG C^GGCCG EcoRV 1302 GATATC GAT^ATC FbaI 6352 TGATCA T^GATCA FseI 1926 GGCCGGCC GGCCGG^CC KpnI 6033 GGTACC GGTAC^C MroI 382 TCCGGA T^CCGGA MvnI 3517 CGCG CG^CG NaeI 1927 GCCGGC GCC^GGC NgoMIV 1927 GCCGGC G^CCGGC NspV 7551 TTCGAA TT^CGAA PaeR7I 3325 CTCGAG C^TCGAG PshAI 3493 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 7987 ATTAAT AT^TAAT Psp1406I 5411 AACGTT AA^CGTT RcaI 448 TCATGA T^CATGA SalI 6700 GTCGAC G^TCGAC ScaI 5320 AGTACT AGT^ACT SfiI 3977 GGCCGGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 7551 TTCGAA TT^CGAA XhoI 3325 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AflII 1311 CTTAAG C^TTAAG 3339 CTTAAG Alw44I 4169 GTGCAC G^TGCAC 7183 GTGCAC AocI 3903 CCTCAGG CC^T[ACGT]AGG 4881 CCTGAGG ApaLI 4169 GTGCAC G^TGCAC 7183 GTGCAC AspI 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACAGTGTC BbeI 6797 GGCGCC GGCGC^C 7242 GGCGCC BbrPI 2463 CACGTG CAC^GTG 6870 CACGTG BfrI 1311 CTTAAG C^TTAAG 3339 CTTAAG BglII 946 AGATCT A^GATCT 3380 AGATCT BsaWI 382 TCCGGA [AT]^CCGG[AT] 2481 ACCGGA BseRI 3901 CTCCTC GAGGAG(10/8) 5502 GAGGAG BsrBI 35 GAGCGG CCGCTC(-3/-3) 213 GAGCGG BsrFI 1927 GCCGGC [GA]^CCGG[CT] 2496 GCCGGT Bsu36I 3903 CCTCAGG CC^T[ACGT]AGG 4881 CCTGAGG Cfr10I 1927 GCCGGC [GA]^CCGG[CT] 2496 GCCGGT Eco81I 3903 CCTCAGG CC^T[ACGT]AGG 4881 CCTGAGG HincII 1397 GTCAAC GT[CT]^[GA]AC 6700 GTCGAC HindII 1397 GTCAAC GT[CT]^[GA]AC 6700 GTCGAC KasI 6797 GGCGCC G^GCGCC 7242 GGCGCC NarI 6797 GGCGCC GG^CGCC 7242 GGCGCC PflFI 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACAGTGTC PmaCI 2463 CACGTG CAC^GTG 6870 CACGTG PmlI 2463 CACGTG CAC^GTG 6870 CACGTG SexAI 2617 ACCTGGT A^CC[AT]GGT 7519 ACCTGGT SfoI 6797 GGCGCC GGC^GCC 7242 GGCGCC SmaI 3351 CCCGGG CCC^GGG 4129 CCCGGG Tth111I 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACAGTGTC XbaI 2670 TCTAGA T^CTAGA 3290 TCTAGA XmaCI 3351 CCCGGG C^CCGGG 4129 CCCGGG XmaI 3351 CCCGGG C^CCGGG 4129 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 146 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI Aor51HI ApaI ApoI Asp700I AspHI AvaI AvaII AviII AvrII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BlnI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstAPI BstEII BstF5I BstNI BstPI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DraIII DrdI DsaI EaeI EarI Eco47III EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NheI NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmnI