##################################
# NO CUT ENZYMES ( 39 enzymes) #
##################################
AatII AgeI AscI BcgI BciVI
BsaBI BsiWI BspDI BssHII Bst1107I
BstZ17I ClaI CpoI DraI EcoRI
EcoT22I HpaI KspI MamI MfeI
MluI MunI NdeI NotI NruI
NsiI PacI PinAI PmeI PvuI
RsrII SacII SbfI SgrAI SnaBI
SpeI Sse8387I SspI SwaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 6033 GGTACC G^GTACC
AccII 3517 CGCG CG^CG
AccIII 382 TCCGGA T^CCGGA
AclI 5411 AACGTT AA^CGTT
AflIII 4270 ACATGT A^C[GA][CT]GT
AhdI 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 7987 ATTAAT AT^TAAT
AsnI 7987 ATTAAT AT^TAAT
Asp718I 6033 GGTACC G^GTACC
AspEI 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 217 GGATCC G^GATCC
BclI 6352 TGATCA T^GATCA
BseAI 382 TCCGGA T^CCGGA
BsmI 6218 GAATGC GAATGC(1/-1)
BspEI 382 TCCGGA T^CCGGA
BspHI 448 TCATGA T^CATGA
BspLU11I 4270 ACATGT A^CATGT
BssSI 769 CACGAG CACGAG(-5/-1)
BstBI 7551 TTCGAA TT^CGAA
BstUI 3517 CGCG CG^CG
EagI 3926 CGGCCG C^GGCCG
Eam1105I 1377 GACTCAGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 3926 CGGCCG C^GGCCG
Eco52I 3926 CGGCCG C^GGCCG
EcoRV 1302 GATATC GAT^ATC
FbaI 6352 TGATCA T^GATCA
FseI 1926 GGCCGGCC GGCCGG^CC
KpnI 6033 GGTACC GGTAC^C
MroI 382 TCCGGA T^CCGGA
MvnI 3517 CGCG CG^CG
NaeI 1927 GCCGGC GCC^GGC
NgoMIV 1927 GCCGGC G^CCGGC
NspV 7551 TTCGAA TT^CGAA
PaeR7I 3325 CTCGAG C^TCGAG
PshAI 3493 GACTAAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 7987 ATTAAT AT^TAAT
Psp1406I 5411 AACGTT AA^CGTT
RcaI 448 TCATGA T^CATGA
SalI 6700 GTCGAC G^TCGAC
ScaI 5320 AGTACT AGT^ACT
SfiI 3977 GGCCGGACAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 7551 TTCGAA TT^CGAA
XhoI 3325 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflII 1311 CTTAAG C^TTAAG
3339 CTTAAG
Alw44I 4169 GTGCAC G^TGCAC
7183 GTGCAC
AocI 3903 CCTCAGG CC^T[ACGT]AGG
4881 CCTGAGG
ApaLI 4169 GTGCAC G^TGCAC
7183 GTGCAC
AspI 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACAGTGTC
BbeI 6797 GGCGCC GGCGC^C
7242 GGCGCC
BbrPI 2463 CACGTG CAC^GTG
6870 CACGTG
BfrI 1311 CTTAAG C^TTAAG
3339 CTTAAG
BglII 946 AGATCT A^GATCT
3380 AGATCT
BsaWI 382 TCCGGA [AT]^CCGG[AT]
2481 ACCGGA
BseRI 3901 CTCCTC GAGGAG(10/8)
5502 GAGGAG
BsrBI 35 GAGCGG CCGCTC(-3/-3)
213 GAGCGG
BsrFI 1927 GCCGGC [GA]^CCGG[CT]
2496 GCCGGT
Bsu36I 3903 CCTCAGG CC^T[ACGT]AGG
4881 CCTGAGG
Cfr10I 1927 GCCGGC [GA]^CCGG[CT]
2496 GCCGGT
Eco81I 3903 CCTCAGG CC^T[ACGT]AGG
4881 CCTGAGG
HincII 1397 GTCAAC GT[CT]^[GA]AC
6700 GTCGAC
HindII 1397 GTCAAC GT[CT]^[GA]AC
6700 GTCGAC
KasI 6797 GGCGCC G^GCGCC
7242 GGCGCC
NarI 6797 GGCGCC GG^CGCC
7242 GGCGCC
PflFI 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACAGTGTC
PmaCI 2463 CACGTG CAC^GTG
6870 CACGTG
PmlI 2463 CACGTG CAC^GTG
6870 CACGTG
SexAI 2617 ACCTGGT A^CC[AT]GGT
7519 ACCTGGT
SfoI 6797 GGCGCC GGC^GCC
7242 GGCGCC
SmaI 3351 CCCGGG CCC^GGG
4129 CCCGGG
Tth111I 2616 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACAGTGTC
XbaI 2670 TCTAGA T^CTAGA
3290 TCTAGA
XmaCI 3351 CCCGGG C^CCGGG
4129 CCCGGG
XmaI 3351 CCCGGG C^CCGGG
4129 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 146 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI Aor51HI ApaI
ApoI Asp700I AspHI AvaI AvaII
AviII AvrII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BlnI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaHI BsaI
BsaJI BsgI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstAPI BstEII BstF5I
BstNI BstPI BstXI BstYI Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII DraIII DrdI DsaI EaeI
EarI Eco47III EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
FspI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NcoI NdeII NheI NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI SspBI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI XhoII
XmnI