##################################
# NO CUT ENZYMES ( 100 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AocI Aor51HI ApaI AscI AseI
AsnI Asp718I AviII AvrII BalI
BamHI BanII BbeI BbvCI BcgI
BglI BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsiEI BsiWI BssHII
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII CpoI
DraII DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoO109I
EcoO65I EcoRV EcoT22I FauI FseI
FspI Hin1I HindIII KasI KpnI
KspI MamI MfeI MluI MluNI
MscI MunI MvnI NaeI NarI
NgoMIV NheI NotI NruI NsiI
NspV PacI PflMI PmeI PpuMI
PshAI PshBI PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SphI Sse8387I SwaI Van91I
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 693 TCCGGA T^CCGGA
AflII 1172 CTTAAG C^TTAAG
AgeI 2295 ACCGGT A^CCGGT
Alw44I 1586 GTGCAC G^TGCAC
ApaLI 1586 GTGCAC G^TGCAC
Asp700I 2975 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 1586 GTGCAC G[AT]GC[AT]^C
AspI 2207 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 487 GGCACC G^G[CT][GA]CC
BbrPI 2256 CACGTG CAC^GTG
BciVI 1000 GGATAC GTATCC(6/5)
BclI 175 TGATCA T^GATCA
BfrI 1172 CTTAAG C^TTAAG
BglII 1362 AGATCT A^GATCT
BsaAI 2256 CACGTG [CT]AC^GT[GA]
BseAI 693 TCCGGA T^CCGGA
BsiHKAI 1586 GTGCAC G[AT]GC[AT]^C
BsmBI 894 GAGACG CGTCTC(1/5)
BspDI 1575 ATCGAT AT^CGAT
BspEI 693 TCCGGA T^CCGGA
BspHI 601 TCATGA T^CATGA
BspMI 490 ACCTGC ACCTGC(4/8)
BsrBI 540 GAGCGG CCGCTC(-3/-3)
BsrDI 1041 GCAATG GCAATG(2/0)
BsrFI 2295 ACCGGT [GA]^CCGG[CT]
BssSI 2945 CTCGTG CACGAG(-5/-1)
BstXI 1245 CCAAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 2295 ACCGGT [GA]^CCGG[CT]
ClaI 1575 ATCGAT AT^CGAT
DsaI 3102 CCATGG C^C[GA][CT]GG
EcoNI 555 CCTTTGTTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2515 GAATTC G^AATTC
FbaI 175 TGATCA T^GATCA
HpaI 2766 GTTAAC GTT^AAC
HphI 406 GGTGA GGTGA(8/7)
MroI 693 TCCGGA T^CCGGA
NcoI 3102 CCATGG C^CATGG
NdeI 1655 CATATG CA^TATG
PaeR7I 2248 CTCGAG C^TCGAG
PflFI 2207 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 2295 ACCGGT A^CCGGT
PmaCI 2256 CACGTG CAC^GTG
PmlI 2256 CACGTG CAC^GTG
RcaI 601 TCATGA T^CATGA
SexAI 3169 ACCTGGT A^CC[AT]GGT
SmaI 2075 CCCGGG CCC^GGG
SspI 3009 AATATT AAT^ATT
Tth111I 2207 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 2895 TCTAGA T^CTAGA
XhoI 2248 CTCGAG C^TCGAG
XmaCI 2075 CCCGGG C^CCGGG
XmaI 2075 CCCGGG C^CCGGG
XmnI 2975 GAACATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AclI 763 AACGTT AA^CGTT
1968 AACGTT
AhdI 1379 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3215 GACACACTGTC
AlwNI 2086 CAGTGCCTG CAG[ACGT][ACGT][ACGT]^CTG
2154 CAGCCGCTG
AspEI 1379 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3215 GACACACTGTC
AvaI 2075 CCCGGG C^[CT]CG[GA]G
2248 CTCGAG
BcnI 2075 CCCGG CC^[GC]GG
2076 CCGGG
BmyI 314 GGGCAC G[AGT]GC[ACT]^C
1586 GTGCAC
BsaWI 693 TCCGGA [AT]^CCGG[AT]
2295 ACCGGT
BsgI 2783 GTGCAG GTGCAG(16/14)
3107 GTGCAG
BsmI 1120 GAATGC GAATGC(1/-1)
830 GCATTC
BsoBI 2075 CCCGGG C^[CT]CG[GA]G
2248 CTCGAG
Bsp1286I 314 GGGCAC G[AGT]GC[ACT]^C
1586 GTGCAC
BspLU11I 3089 ACATGT A^CATGT
3143 ACATGT
DraI 620 TTTAAA TTT^AAA
2601 TTTAAA
Eam1105I 1379 GACGTGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3215 GACACACTGTC
HaeII 338 GGCGCT [GA]GCGC^[CT]
2656 GGCGCT
HgaI 1548 GACGC GACGC(5/10)
2100 GCGTC
NciI 2075 CCCGG CC^[GC]GG
2076 CCGGG
Psp1406I 763 AACGTT AA^CGTT
1968 AACGTT
PvuII 1592 CAGCTG CAG^CTG
2114 CAGCTG
ScaI 89 AGTACT AGT^ACT
1029 AGTACT
SmlI 1172 CTTAAG C^T[CT][GA]AG
2248 CTCGAG
StuI 1536 AGGCCT AGG^CCT
2446 AGGCCT
TfiI 382 GAATC G^A[AT]TC
409 GAATC
XcmI 1408 CCAGTGCCTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3007 CCAATATTTTTTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI ApoI AvaII BbsI BbvI
BfaI BpmI BpuAI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1407I
BsrGI BsrI BssKI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SspBI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII