################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AatII Acc65I AccIII AflII AgeI Alw44I ApaI ApaLI AscI AseI AsnI Asp700I Asp718I BalI BamHI BcgI BciVI BfrI BsaBI BsaWI BseAI BsiEI Bsp1407I BspDI BspEI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraIII EaeI EagI EclXI Eco52I EcoO65I EcoRI EcoRV EcoT22I FseI HincII HindII HpaI KpnI KspI MamI MfeI MluI MluNI MroI MscI MunI NcoI NdeI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI StuI SwaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AclI 2941 AACGTT AA^CGTT AcyI 3371 GGCGCC G[GA]^CG[CT]C AhdI 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 2271 CCTCAGG CC^T[ACGT]AGG AspEI 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 988 CTCGGG C^[CT]CG[GA]G AviII 710 TGCGCA TGC^GCA BbeI 3371 GGCGCC GGCGC^C BclI 3287 TGATCA T^GATCA BglI 2364 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaHI 3371 GGCGCC G[GA]^CG[CT]C BsgI 3444 CTGCAC GTGCAG(16/14) BsiWI 2873 CGTACG C^GTACG BsmBI 3376 CGTCTC CGTCTC(1/5) BsmI 1193 GAATGC GAATGC(1/-1) BsoBI 988 CTCGGG C^[CT]CG[GA]G BspHI 394 TCATGA T^CATGA BspLU11I 1204 ACATGT A^CATGT BspMI 683 GCAGGT ACCTGC(4/8) BsrBI 1446 GAGCGG CCGCTC(-3/-3) BssSI 2974 CTCGTG CACGAG(-5/-1) Bst1107I 2447 GTATAC GTA^TAC BstAPI 1383 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstZ17I 2447 GTATAC GTA^TAC Bsu36I 2271 CCTCAGG CC^T[ACGT]AGG DrdI 1152 GACAGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco81I 2271 CCTCAGG CC^T[ACGT]AGG FauI 1607 GCGGG CCCGC(4/6) FbaI 3287 TGATCA T^GATCA FspI 710 TGCGCA TGC^GCA Hin1I 3371 GGCGCC G[GA]^CG[CT]C HindIII 3490 AAGCTT A^AGCTT KasI 3371 GGCGCC G^GCGCC NaeI 1273 GCCGGC GCC^GGC NarI 3371 GGCGCC GG^CGCC NgoMIV 1273 GCCGGC G^CCGGC NheI 2318 GCTAGC G^CTAGC NspI 1204 ACATGT [GA]CATG^[CT] PflFI 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2941 AACGTT AA^CGTT PstI 2097 CTGCAG CTGCA^G RcaI 394 TCATGA T^CATGA SacI 417 GAGCTC GAGCT^C ScaI 1498 AGTACT AGT^ACT SfoI 3371 GGCGCC GGC^GCC SspI 2126 AATATT AAT^ATT Tth111I 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 536 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 2189 GTCTAC GT^[AC][GT]AC 2447 GTATAC AccII 2067 CGCG CG^CG 2662 CGCG AlwI 428 GATCC GGATC(4/5) 3472 GATCC Aor51HI 132 AGCGCT AGC^GCT 2970 AGCGCT AvrII 2012 CCTAGG C^CTAGG 2864 CCTAGG BanII 417 GAGCTC G[GA]GC[CT]^C 453 GAGCCC BbrPI 2041 CACGTG CAC^GTG 2811 CACGTG BbvCI 2788 CCTCAGC CCTCAGC(-5/-2) 3157 CCTCAGC BglII 485 AGATCT A^GATCT 1899 AGATCT BlnI 2012 CCTAGG C^CTAGG 2864 CCTAGG BlpI 1890 GCTCAGC GC^T[ACGT]AGC 2741 GCTGAGC Bpu1102I 1890 GCTCAGC GC^T[ACGT]AGC 2741 GCTGAGC BsaAI 2041 CACGTG [CT]AC^GT[GA] 2811 CACGTG BsrFI 437 GCCGGT [GA]^CCGG[CT] 1273 GCCGGC BstUI 2067 CGCG CG^CG 2662 CGCG BstXI 827 CCACTCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2505 CCAGTAGAATGG CelII 1890 GCTCAGC GC^T[ACGT]AGC 2741 GCTGAGC Cfr10I 437 GCCGGT [GA]^CCGG[CT] 1273 GCCGGC Eco47III 132 AGCGCT AGC^GCT 2970 AGCGCT EcoNI 2246 CCTCATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2859 CCTTCCCTAGG HgaI 1741 GACGC GACGC(5/10) 746 GCGTC MvnI 2067 CGCG CG^CG 2662 CGCG PflMI 2411 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3361 CCATAAAATGG PmaCI 2041 CACGTG CAC^GTG 2811 CACGTG PmlI 2041 CACGTG CAC^GTG 2811 CACGTG PpuMI 1456 AGGACCC [GA]G^G[AT]CC[CT] 3170 GGGTCCT SmlI 51 CTTGAG C^T[CT][GA]AG 2654 CTCAAG TaqI 776 TCGA T^CGA 1751 TCGA TthHB8I 776 TCGA T^CGA 1751 TCGA Van91I 2411 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3361 CCATAAAATGG XcmI 248 CCACAAACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1481 CCATATTTATAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwNI ApoI AspHI AvaII BanI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII