##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I BalI BamHI
BcgI BciVI BfrI BsaBI BsaWI
BseAI BsiEI Bsp1407I BspDI BspEI
BsrGI BssHII BstBI BstEII BstPI
ClaI CpoI DraIII EaeI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
EcoT22I FseI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MluNI MroI MscI MunI NcoI
NdeI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI PvuI RsrII SacII SalI
SapI SbfI SexAI SfiI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI StuI SwaI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AclI 2941 AACGTT AA^CGTT
AcyI 3371 GGCGCC G[GA]^CG[CT]C
AhdI 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 2271 CCTCAGG CC^T[ACGT]AGG
AspEI 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 988 CTCGGG C^[CT]CG[GA]G
AviII 710 TGCGCA TGC^GCA
BbeI 3371 GGCGCC GGCGC^C
BclI 3287 TGATCA T^GATCA
BglI 2364 GCCTTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 3371 GGCGCC G[GA]^CG[CT]C
BsgI 3444 CTGCAC GTGCAG(16/14)
BsiWI 2873 CGTACG C^GTACG
BsmBI 3376 CGTCTC CGTCTC(1/5)
BsmI 1193 GAATGC GAATGC(1/-1)
BsoBI 988 CTCGGG C^[CT]CG[GA]G
BspHI 394 TCATGA T^CATGA
BspLU11I 1204 ACATGT A^CATGT
BspMI 683 GCAGGT ACCTGC(4/8)
BsrBI 1446 GAGCGG CCGCTC(-3/-3)
BssSI 2974 CTCGTG CACGAG(-5/-1)
Bst1107I 2447 GTATAC GTA^TAC
BstAPI 1383 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstZ17I 2447 GTATAC GTA^TAC
Bsu36I 2271 CCTCAGG CC^T[ACGT]AGG
DrdI 1152 GACAGCAGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 740 GACATTGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 2271 CCTCAGG CC^T[ACGT]AGG
FauI 1607 GCGGG CCCGC(4/6)
FbaI 3287 TGATCA T^GATCA
FspI 710 TGCGCA TGC^GCA
Hin1I 3371 GGCGCC G[GA]^CG[CT]C
HindIII 3490 AAGCTT A^AGCTT
KasI 3371 GGCGCC G^GCGCC
NaeI 1273 GCCGGC GCC^GGC
NarI 3371 GGCGCC GG^CGCC
NgoMIV 1273 GCCGGC G^CCGGC
NheI 2318 GCTAGC G^CTAGC
NspI 1204 ACATGT [GA]CATG^[CT]
PflFI 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2941 AACGTT AA^CGTT
PstI 2097 CTGCAG CTGCA^G
RcaI 394 TCATGA T^CATGA
SacI 417 GAGCTC GAGCT^C
ScaI 1498 AGTACT AGT^ACT
SfoI 3371 GGCGCC GGC^GCC
SspI 2126 AATATT AAT^ATT
Tth111I 1458 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 536 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2189 GTCTAC GT^[AC][GT]AC
2447 GTATAC
AccII 2067 CGCG CG^CG
2662 CGCG
AlwI 428 GATCC GGATC(4/5)
3472 GATCC
Aor51HI 132 AGCGCT AGC^GCT
2970 AGCGCT
AvrII 2012 CCTAGG C^CTAGG
2864 CCTAGG
BanII 417 GAGCTC G[GA]GC[CT]^C
453 GAGCCC
BbrPI 2041 CACGTG CAC^GTG
2811 CACGTG
BbvCI 2788 CCTCAGC CCTCAGC(-5/-2)
3157 CCTCAGC
BglII 485 AGATCT A^GATCT
1899 AGATCT
BlnI 2012 CCTAGG C^CTAGG
2864 CCTAGG
BlpI 1890 GCTCAGC GC^T[ACGT]AGC
2741 GCTGAGC
Bpu1102I 1890 GCTCAGC GC^T[ACGT]AGC
2741 GCTGAGC
BsaAI 2041 CACGTG [CT]AC^GT[GA]
2811 CACGTG
BsrFI 437 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
BstUI 2067 CGCG CG^CG
2662 CGCG
BstXI 827 CCACTCAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2505 CCAGTAGAATGG
CelII 1890 GCTCAGC GC^T[ACGT]AGC
2741 GCTGAGC
Cfr10I 437 GCCGGT [GA]^CCGG[CT]
1273 GCCGGC
Eco47III 132 AGCGCT AGC^GCT
2970 AGCGCT
EcoNI 2246 CCTCATAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2859 CCTTCCCTAGG
HgaI 1741 GACGC GACGC(5/10)
746 GCGTC
MvnI 2067 CGCG CG^CG
2662 CGCG
PflMI 2411 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCATAAAATGG
PmaCI 2041 CACGTG CAC^GTG
2811 CACGTG
PmlI 2041 CACGTG CAC^GTG
2811 CACGTG
PpuMI 1456 AGGACCC [GA]G^G[AT]CC[CT]
3170 GGGTCCT
SmlI 51 CTTGAG C^T[CT][GA]AG
2654 CTCAAG
TaqI 776 TCGA T^CGA
1751 TCGA
TthHB8I 776 TCGA T^CGA
1751 TCGA
Van91I 2411 CCACAGTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3361 CCATAAAATGG
XcmI 248 CCACAAACGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1481 CCATATTTATAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwNI ApoI AspHI AvaII BanI
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrI BssKI BstF5I
BstNI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraI DraII DsaI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI XhoII