##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BcgI BclI BfrI
BlnI BsaBI BseAI BsiWI BspDI
BspEI BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI CpoI
DrdI Eco81I EcoNI EcoO65I EcoRV
FbaI FseI FspI HincII HindII
HpaI KpnI MamI MluI MluNI
MroI MscI NdeI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SalI SexAI SfiI SfuI
SnaBI SpeI SwaI Tth111I Van91I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AflIII 1090 ACATGT A^C[GA][CT]GT
AhdI 2039 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 296 AGCGCT AGC^GCT
ApaI 250 GGGCCC GGGCC^C
AspEI 2039 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 51 GGATCC G^GATCC
BbeI 1311 GGCGCC GGCGC^C
BbrPI 1633 CACGTG CAC^GTG
BciVI 778 GGATAC GTATCC(6/5)
BglII 1677 AGATCT A^GATCT
BsaAI 1633 CACGTG [CT]AC^GT[GA]
BsaI 2333 GAGACC GGTCTC(1/5)
BsmI 2892 GCATTC GAATGC(1/-1)
BspHI 880 TCATGA T^CATGA
BspLU11I 1090 ACATGT A^CATGT
BssHII 436 GCGCGC G^CGCGC
BstAPI 2014 GCACTCGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2624 CCATTCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraI 146 TTTAAA TTT^AAA
DraIII 2446 CACATCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2039 GACATCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 296 AGCGCT AGC^GCT
EcoRI 2619 GAATTC G^AATTC
EcoT22I 3239 ATGCAT ATGCA^T
HindIII 2922 AAGCTT A^AGCTT
KasI 1311 GGCGCC G^GCGCC
MfeI 763 CAATTG C^AATTG
MunI 763 CAATTG C^AATTG
NarI 1311 GGCGCC GG^CGCC
NcoI 2910 CCATGG C^CATGG
NheI 1489 GCTAGC G^CTAGC
NsiI 3239 ATGCAT ATGCA^T
PmaCI 1633 CACGTG CAC^GTG
PmlI 1633 CACGTG CAC^GTG
RcaI 880 TCATGA T^CATGA
SacI 1944 GAGCTC GAGCT^C
SapI 367 GCTCTTC GCTCTTC(1/4)
SbfI 1865 CCTGCAGG CCTGCA^GG
ScaI 1452 AGTACT AGT^ACT
SfoI 1311 GGCGCC GGC^GCC
SgrAI 196 CGCCGGTG C[GA]^CCGG[CT]G
SphI 2400 GCATGC GCATG^C
Sse8387I 1865 CCTGCAGG CCTGCA^GG
SspI 1460 AATATT AAT^ATT
StuI 1260 AGGCCT AGG^CCT
XcmI 989 CCACCTCATAACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1586 GTAGAC GT^[AC][GT]AC
2753 GTCTAC
AlwNI 497 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
1328 CAGGAACTG
Asp700I 569 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2976 GAATATCTTC
AspHI 1944 GAGCTC G[AT]GC[AT]^C
2452 GTGCTC
BbvCI 2457 CCTCAGC CCTCAGC(-5/-2)
1279 GCTGAGG
BglI 386 GCCGGCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
452 GCCGGAGCGGC
BlpI 1303 GCTGAGC GC^T[ACGT]AGC
1946 GCTCAGC
Bpu1102I 1303 GCTGAGC GC^T[ACGT]AGC
1946 GCTCAGC
BsaWI 154 ACCGGA [AT]^CCGG[AT]
2813 ACCGGA
BsiHKAI 1944 GAGCTC G[AT]GC[AT]^C
2452 GTGCTC
Bsp1407I 2370 TGTACA T^GTACA
2882 TGTACA
BsrDI 359 GCAATG GCAATG(2/0)
2466 GCAATG
BsrGI 2370 TGTACA T^GTACA
2882 TGTACA
CelII 1303 GCTGAGC GC^T[ACGT]AGC
1946 GCTCAGC
EagI 245 CGGCCG C^GGCCG
450 CGGCCG
EclXI 245 CGGCCG C^GGCCG
450 CGGCCG
Eco52I 245 CGGCCG C^GGCCG
450 CGGCCG
EcoT14I 1251 CCAAGG C^C[AT][AT]GG
2910 CCATGG
KspI 65 CCGCGG CCGC^GG
427 CCGCGG
PpuMI 1547 GGGACCC [GA]G^G[AT]CC[CT]
3456 GGGACCC
PshAI 920 GACCGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2528 GACCTCTGTC
SacII 65 CCGCGG CCGC^GG
427 CCGCGG
SmaI 254 CCCGGG CCC^GGG
2301 CCCGGG
SspBI 2370 TGTACA T^GTACA
2882 TGTACA
StyI 1251 CCAAGG C^C[AT][AT]GG
2910 CCATGG
TfiI 1597 GATTC G^A[AT]TC
3375 GATTC
XmaCI 254 CCCGGG C^CCGGG
2301 CCCGGG
XmaI 254 CCCGGG C^CCGGG
2301 CCCGGG
XmnI 569 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2976 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiEI BsiYI
BslI BsmAI BsmBI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NciI NdeII NgoMIV
NlaIII NlaIV NspI PleI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII