##################################
# NO CUT ENZYMES ( 55 enzymes) #
##################################
AatII AclI AgeI AscI AspI
AvrII BbeI BlnI BsiWI Bsp1407I
BsrGI BssHII Bst1107I BstBI BstZ17I
CpoI DrdI EagI EclXI Eco52I
EcoRV FseI KasI KspI MfeI
MluI MunI NaeI NarI NgoMIV
NotI NruI NspV PacI PaeR7I
PflFI PinAI PmeI Psp1406I PvuI
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SpeI Sse8387I
SspBI SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AccIII 2587 TCCGGA T^CCGGA
AflII 7871 CTTAAG C^TTAAG
AflIII 8634 ACATGT A^C[GA][CT]GT
AseI 4376 ATTAAT AT^TAAT
AsnI 4376 ATTAAT AT^TAAT
AviII 360 TGCGCA TGC^GCA
BbvCI 6630 GCTGAGG CCTCAGC(-5/-2)
BcgI 1521 GCACGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 7871 CTTAAG C^TTAAG
BlpI 7449 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 7449 GCTCAGC GC^T[ACGT]AGC
BseAI 2587 TCCGGA T^CCGGA
BsiEI 1959 CGACCG CG[GA][CT]^CG
BsmBI 7255 CGTCTC CGTCTC(1/5)
BspDI 1589 ATCGAT AT^CGAT
BspEI 2587 TCCGGA T^CCGGA
BspLU11I 8634 ACATGT A^CATGT
BsrFI 1944 ACCGGC [GA]^CCGG[CT]
CelII 7449 GCTCAGC GC^T[ACGT]AGC
Cfr10I 1944 ACCGGC [GA]^CCGG[CT]
ClaI 1589 ATCGAT AT^CGAT
FspI 360 TGCGCA TGC^GCA
HpaI 7072 GTTAAC GTT^AAC
MroI 2587 TCCGGA T^CCGGA
NdeI 7726 CATATG CA^TATG
PshBI 4376 ATTAAT AT^TAAT
SexAI 3791 ACCTGGT A^CC[AT]GGT
SmaI 2963 CCCGGG CCC^GGG
SnaBI 5411 TACGTA TAC^GTA
SphI 4092 GCATGC GCATG^C
XmaCI 2963 CCCGGG C^CCGGG
XmaI 2963 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 416 GGTACC G^GTACC
1210 GGTACC
AccII 2604 CGCG CG^CG
7588 CGCG
Aor51HI 2361 AGCGCT AGC^GCT
3029 AGCGCT
ApaI 2444 GGGCCC GGGCC^C
2781 GGGCCC
Asp700I 4945 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6205 GAAAGGCTTC
Asp718I 416 GGTACC G^GTACC
1210 GGTACC
BbrPI 1057 CACGTG CAC^GTG
1406 CACGTG
BclI 2100 TGATCA T^GATCA
6978 TGATCA
BsaBI 1510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3318 GATAGCAATC
BsmI 3823 GAATGC GAATGC(1/-1)
3375 GCATTC
BstEII 1972 GGTGACC G^GT[ACGT]ACC
4609 GGTAACC
BstPI 1972 GGTGACC G^GT[ACGT]ACC
4609 GGTAACC
BstUI 2604 CGCG CG^CG
7588 CGCG
Eco47III 2361 AGCGCT AGC^GCT
3029 AGCGCT
EcoNI 1609 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3042 CCTCCCCCAGG
EcoO65I 1972 GGTGACC G^GT[ACGT]ACC
4609 GGTAACC
FbaI 2100 TGATCA T^GATCA
6978 TGATCA
HincII 1796 GTCAAC GT[CT]^[GA]AC
7072 GTTAAC
HindII 1796 GTCAAC GT[CT]^[GA]AC
7072 GTTAAC
KpnI 416 GGTACC GGTAC^C
1210 GGTACC
MamI 1510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
3318 GATAGCAATC
MvnI 2604 CGCG CG^CG
7588 CGCG
NheI 424 GCTAGC G^CTAGC
6002 GCTAGC
PmaCI 1057 CACGTG CAC^GTG
1406 CACGTG
PmlI 1057 CACGTG CAC^GTG
1406 CACGTG
PshAI 3664 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
6769 GACTCTAGTC
PstI 2126 CTGCAG CTGCA^G
3574 CTGCAG
SspI 5118 AATATT AAT^ATT
8682 AATATT
StuI 232 AGGCCT AGG^CCT
973 AGGCCT
TaqI 1590 TCGA T^CGA
3504 TCGA
TthHB8I 1590 TCGA T^CGA
3504 TCGA
XmnI 4945 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
6205 GAAAGGCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AhdI AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspEI AspHI
AvaI AvaII BalI BamHI BanI
BanII BbsI BbvI BciVI BcnI
BfaI BglI BglII BmyI BpmI
BpuAI BsaAI BsaHI BsaI BsaJI
BsaWI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BspMI BsrBI BsrDI BsrI
BssKI BssSI BstAPI BstF5I BstNI
BstXI BstYI Bsu36I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DraIII DsaI EaeI Eam1105I EarI
Eco81I EcoO109I EcoRI EcoRII EcoT14I
EcoT22I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NsiI NspI PflMI
PleI PpuMI PvuII RcaI RsaI
SacI SapI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Van91I XcmI XhoII