################################## # NO CUT ENZYMES ( 55 enzymes) # ################################## AatII AclI AgeI AscI AspI AvrII BbeI BlnI BsiWI Bsp1407I BsrGI BssHII Bst1107I BstBI BstZ17I CpoI DrdI EagI EclXI Eco52I EcoRV FseI KasI KspI MfeI MluI MunI NaeI NarI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmeI Psp1406I PvuI RsrII SacII SalI SbfI SfiI SfoI SfuI SgrAI SpeI Sse8387I SspBI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AccIII 2587 TCCGGA T^CCGGA AflII 7871 CTTAAG C^TTAAG AflIII 8634 ACATGT A^C[GA][CT]GT AseI 4376 ATTAAT AT^TAAT AsnI 4376 ATTAAT AT^TAAT AviII 360 TGCGCA TGC^GCA BbvCI 6630 GCTGAGG CCTCAGC(-5/-2) BcgI 1521 GCACGCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 7871 CTTAAG C^TTAAG BlpI 7449 GCTCAGC GC^T[ACGT]AGC Bpu1102I 7449 GCTCAGC GC^T[ACGT]AGC BseAI 2587 TCCGGA T^CCGGA BsiEI 1959 CGACCG CG[GA][CT]^CG BsmBI 7255 CGTCTC CGTCTC(1/5) BspDI 1589 ATCGAT AT^CGAT BspEI 2587 TCCGGA T^CCGGA BspLU11I 8634 ACATGT A^CATGT BsrFI 1944 ACCGGC [GA]^CCGG[CT] CelII 7449 GCTCAGC GC^T[ACGT]AGC Cfr10I 1944 ACCGGC [GA]^CCGG[CT] ClaI 1589 ATCGAT AT^CGAT FspI 360 TGCGCA TGC^GCA HpaI 7072 GTTAAC GTT^AAC MroI 2587 TCCGGA T^CCGGA NdeI 7726 CATATG CA^TATG PshBI 4376 ATTAAT AT^TAAT SexAI 3791 ACCTGGT A^CC[AT]GGT SmaI 2963 CCCGGG CCC^GGG SnaBI 5411 TACGTA TAC^GTA SphI 4092 GCATGC GCATG^C XmaCI 2963 CCCGGG C^CCGGG XmaI 2963 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### Acc65I 416 GGTACC G^GTACC 1210 GGTACC AccII 2604 CGCG CG^CG 7588 CGCG Aor51HI 2361 AGCGCT AGC^GCT 3029 AGCGCT ApaI 2444 GGGCCC GGGCC^C 2781 GGGCCC Asp700I 4945 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6205 GAAAGGCTTC Asp718I 416 GGTACC G^GTACC 1210 GGTACC BbrPI 1057 CACGTG CAC^GTG 1406 CACGTG BclI 2100 TGATCA T^GATCA 6978 TGATCA BsaBI 1510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3318 GATAGCAATC BsmI 3823 GAATGC GAATGC(1/-1) 3375 GCATTC BstEII 1972 GGTGACC G^GT[ACGT]ACC 4609 GGTAACC BstPI 1972 GGTGACC G^GT[ACGT]ACC 4609 GGTAACC BstUI 2604 CGCG CG^CG 7588 CGCG Eco47III 2361 AGCGCT AGC^GCT 3029 AGCGCT EcoNI 1609 CCTGTCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3042 CCTCCCCCAGG EcoO65I 1972 GGTGACC G^GT[ACGT]ACC 4609 GGTAACC FbaI 2100 TGATCA T^GATCA 6978 TGATCA HincII 1796 GTCAAC GT[CT]^[GA]AC 7072 GTTAAC HindII 1796 GTCAAC GT[CT]^[GA]AC 7072 GTTAAC KpnI 416 GGTACC GGTAC^C 1210 GGTACC MamI 1510 GATGTCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 3318 GATAGCAATC MvnI 2604 CGCG CG^CG 7588 CGCG NheI 424 GCTAGC G^CTAGC 6002 GCTAGC PmaCI 1057 CACGTG CAC^GTG 1406 CACGTG PmlI 1057 CACGTG CAC^GTG 1406 CACGTG PshAI 3664 GACTTAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 6769 GACTCTAGTC PstI 2126 CTGCAG CTGCA^G 3574 CTGCAG SspI 5118 AATATT AAT^ATT 8682 AATATT StuI 232 AGGCCT AGG^CCT 973 AGGCCT TaqI 1590 TCGA T^CGA 3504 TCGA TthHB8I 1590 TCGA T^CGA 3504 TCGA XmnI 4945 GAAAGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 6205 GAAAGGCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 139 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AhdI AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspEI AspHI AvaI AvaII BalI BamHI BanI BanII BbsI BbvI BciVI BcnI BfaI BglI BglII BmyI BpmI BpuAI BsaAI BsaHI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BspMI BsrBI BsrDI BsrI BssKI BssSI BstAPI BstF5I BstNI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI Eam1105I EarI Eco81I EcoO109I EcoRI EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NsiI NspI PflMI PleI PpuMI PvuII RcaI RsaI SacI SapI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII