################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII Acc65I AclI AcyI AflII AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BclI BcnI BfrI BglII BlnI BsaBI BsaHI BsiWI BsmBI Bsp1407I BspDI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FbaI FseI FspI Hin1I HindIII HpaI KasI KpnI MamI MfeI MluI MunI NaeI NarI NciI NdeI NgoMIV NotI NruI NspV PacI PflFI PmeI PshAI PshBI Psp1406I PvuI RsrII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SwaI Tth111I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AccI 597 GTCTAC GT^[AC][GT]AC AcsI 268 AAATTT [GA]^AATT[CT] AflIII 2319 ACATGT A^C[GA][CT]GT AgeI 2830 ACCGGT A^CCGGT Alw44I 2200 GTGCAC G^TGCAC ApaLI 2200 GTGCAC G^TGCAC ApoI 268 AAATTT [GA]^AATT[CT] AvaI 2703 CTCGAG C^[CT]CG[GA]G BbrPI 2487 CACGTG CAC^GTG BbsI 1150 GAAGAC GAAGAC(2/6) BcgI 1200 CGAGATGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1150 GAAGAC GAAGAC(2/6) BsaAI 2487 CACGTG [CT]AC^GT[GA] BsoBI 2703 CTCGAG C^[CT]CG[GA]G BspHI 1807 TCATGA T^CATGA BspLU11I 2319 ACATGT A^CATGT BsrBI 1369 CCGCTC CCGCTC(-3/-3) BssSI 454 CACGAG CACGAG(-5/-1) DrdI 67 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1164 ATGCAT ATGCA^T HgaI 102 GCGTC GACGC(5/10) KspI 957 CCGCGG CCGC^GG NheI 2431 GCTAGC G^CTAGC NsiI 1164 ATGCAT ATGCA^T PaeR7I 2703 CTCGAG C^TCGAG PinAI 2830 ACCGGT A^CCGGT PmaCI 2487 CACGTG CAC^GTG PmlI 2487 CACGTG CAC^GTG RcaI 1807 TCATGA T^CATGA SacII 957 CCGCGG CCGC^GG SapI 97 GAAGAGC GCTCTTC(1/4) SexAI 1052 ACCAGGT A^CC[AT]GGT SphI 2435 GCATGC GCATG^C XbaI 2113 TCTAGA T^CTAGA XhoI 2703 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccIII 749 TCCGGA T^CCGGA 1137 TCCGGA AocI 1306 CCTGAGG CC^T[ACGT]AGG 1872 CCTCAGG ApaI 1752 GGGCCC GGGCC^C 1877 GGGCCC BbvCI 1512 CCTCAGC CCTCAGC(-5/-2) 1527 GCTGAGG BciVI 2659 GGATAC GTATCC(6/5) 986 GTATCC BlpI 1517 GCTCAGC GC^T[ACGT]AGC 2565 GCTCAGC Bpu1102I 1517 GCTCAGC GC^T[ACGT]AGC 2565 GCTCAGC BseAI 749 TCCGGA T^CCGGA 1137 TCCGGA BsiEI 24 CGGTCG CG[GA][CT]^CG 41 CGGTCG BsmI 1599 GAATGC GAATGC(1/-1) 2511 GAATGC BspEI 749 TCCGGA T^CCGGA 1137 TCCGGA BsrFI 39 GCCGGT [GA]^CCGG[CT] 2830 ACCGGT Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG 1872 CCTCAGG CelII 1517 GCTCAGC GC^T[ACGT]AGC 2565 GCTCAGC CfoI 894 GCGC GCG^C 1313 GCGC Cfr10I 39 GCCGGT [GA]^CCGG[CT] 2830 ACCGGT Eco81I 1306 CCTGAGG CC^T[ACGT]AGG 1872 CCTCAGG HaeII 893 GGCGCT [GA]GCGC^[CT] 1312 GGCGCT HhaI 894 GCGC GCG^C 1313 GCGC HinP1I 894 GCGC G^CGC 1313 GCGC HincII 2592 GTCAAC GT[CT]^[GA]AC 2990 GTTGAC HindII 2592 GTCAAC GT[CT]^[GA]AC 2990 GTTGAC MaeII 1703 ACGT A^CGT 2488 ACGT MroI 749 TCCGGA T^CCGGA 1137 TCCGGA PpuMI 428 AGGTCCT [GA]G^G[AT]CC[CT] 2543 GGGTCCC PstI 92 CTGCAG CTGCA^G 3064 CTGCAG PvuII 1488 CAGCTG CAG^CTG 1498 CAGCTG SspI 2954 AATATT AAT^ATT 3008 AATATT TaqI 308 TCGA T^CGA 2704 TCGA TfiI 692 GATTC G^A[AT]TC 753 GAATC Tsp509I 195 AATT ^AATT 269 AATT TthHB8I 308 TCGA T^CGA 2704 TCGA XcmI 1917 CCACCCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2494 CCAAGGCCTTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaII BalI BamHI BanI BanII BbvI BfaI BglI BmyI BpmI BsaI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NcoI NdeII NlaIII NlaIV NspI PflMI PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI Tru9I TseI Tsp45I TspRI Van91I XhoII