##################################
# NO CUT ENZYMES ( 91 enzymes) #
##################################
AatII Acc65I AclI AcyI AflII
AhdI Aor51HI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AviII
AvrII BbeI BclI BcnI BfrI
BglII BlnI BsaBI BsaHI BsiWI
BsmBI Bsp1407I BspDI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
EcoRV FbaI FseI FspI Hin1I
HindIII HpaI KasI KpnI MamI
MfeI MluI MunI NaeI NarI
NciI NdeI NgoMIV NotI NruI
NspV PacI PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SalI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SwaI Tth111I XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AccI 597 GTCTAC GT^[AC][GT]AC
AcsI 268 AAATTT [GA]^AATT[CT]
AflIII 2319 ACATGT A^C[GA][CT]GT
AgeI 2830 ACCGGT A^CCGGT
Alw44I 2200 GTGCAC G^TGCAC
ApaLI 2200 GTGCAC G^TGCAC
ApoI 268 AAATTT [GA]^AATT[CT]
AvaI 2703 CTCGAG C^[CT]CG[GA]G
BbrPI 2487 CACGTG CAC^GTG
BbsI 1150 GAAGAC GAAGAC(2/6)
BcgI 1200 CGAGATGCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1150 GAAGAC GAAGAC(2/6)
BsaAI 2487 CACGTG [CT]AC^GT[GA]
BsoBI 2703 CTCGAG C^[CT]CG[GA]G
BspHI 1807 TCATGA T^CATGA
BspLU11I 2319 ACATGT A^CATGT
BsrBI 1369 CCGCTC CCGCTC(-3/-3)
BssSI 454 CACGAG CACGAG(-5/-1)
DrdI 67 GACAGCTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1164 ATGCAT ATGCA^T
HgaI 102 GCGTC GACGC(5/10)
KspI 957 CCGCGG CCGC^GG
NheI 2431 GCTAGC G^CTAGC
NsiI 1164 ATGCAT ATGCA^T
PaeR7I 2703 CTCGAG C^TCGAG
PinAI 2830 ACCGGT A^CCGGT
PmaCI 2487 CACGTG CAC^GTG
PmlI 2487 CACGTG CAC^GTG
RcaI 1807 TCATGA T^CATGA
SacII 957 CCGCGG CCGC^GG
SapI 97 GAAGAGC GCTCTTC(1/4)
SexAI 1052 ACCAGGT A^CC[AT]GGT
SphI 2435 GCATGC GCATG^C
XbaI 2113 TCTAGA T^CTAGA
XhoI 2703 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 749 TCCGGA T^CCGGA
1137 TCCGGA
AocI 1306 CCTGAGG CC^T[ACGT]AGG
1872 CCTCAGG
ApaI 1752 GGGCCC GGGCC^C
1877 GGGCCC
BbvCI 1512 CCTCAGC CCTCAGC(-5/-2)
1527 GCTGAGG
BciVI 2659 GGATAC GTATCC(6/5)
986 GTATCC
BlpI 1517 GCTCAGC GC^T[ACGT]AGC
2565 GCTCAGC
Bpu1102I 1517 GCTCAGC GC^T[ACGT]AGC
2565 GCTCAGC
BseAI 749 TCCGGA T^CCGGA
1137 TCCGGA
BsiEI 24 CGGTCG CG[GA][CT]^CG
41 CGGTCG
BsmI 1599 GAATGC GAATGC(1/-1)
2511 GAATGC
BspEI 749 TCCGGA T^CCGGA
1137 TCCGGA
BsrFI 39 GCCGGT [GA]^CCGG[CT]
2830 ACCGGT
Bsu36I 1306 CCTGAGG CC^T[ACGT]AGG
1872 CCTCAGG
CelII 1517 GCTCAGC GC^T[ACGT]AGC
2565 GCTCAGC
CfoI 894 GCGC GCG^C
1313 GCGC
Cfr10I 39 GCCGGT [GA]^CCGG[CT]
2830 ACCGGT
Eco81I 1306 CCTGAGG CC^T[ACGT]AGG
1872 CCTCAGG
HaeII 893 GGCGCT [GA]GCGC^[CT]
1312 GGCGCT
HhaI 894 GCGC GCG^C
1313 GCGC
HinP1I 894 GCGC G^CGC
1313 GCGC
HincII 2592 GTCAAC GT[CT]^[GA]AC
2990 GTTGAC
HindII 2592 GTCAAC GT[CT]^[GA]AC
2990 GTTGAC
MaeII 1703 ACGT A^CGT
2488 ACGT
MroI 749 TCCGGA T^CCGGA
1137 TCCGGA
PpuMI 428 AGGTCCT [GA]G^G[AT]CC[CT]
2543 GGGTCCC
PstI 92 CTGCAG CTGCA^G
3064 CTGCAG
PvuII 1488 CAGCTG CAG^CTG
1498 CAGCTG
SspI 2954 AATATT AAT^ATT
3008 AATATT
TaqI 308 TCGA T^CGA
2704 TCGA
TfiI 692 GATTC G^A[AT]TC
753 GAATC
Tsp509I 195 AATT ^AATT
269 AATT
TthHB8I 308 TCGA T^CGA
2704 TCGA
XcmI 1917 CCACCCAGTGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2494 CCAAGGCCTTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaII BalI BamHI
BanI BanII BbvI BfaI BglI
BmyI BpmI BsaI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstXI BstYI Cac8I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI Tru9I TseI
Tsp45I TspRI Van91I XhoII