##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AgeI AhdI Alw44I ApaI ApaLI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbeI BglI
BlnI BsaWI BseAI BsiEI BsiWI
BsmI BspDI BspEI BsrBI BsrFI
BssHII BssSI Bst1107I BstEII BstPI
BstUI BstZ17I Cfr10I ClaI CpoI
DsaI EagI Eam1105I EclXI Eco52I
EcoNI EcoO65I EcoT22I FseI FspI
HincII HindII HpaI KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NcoI NdeI NgoMIV
NotI NruI NsiI PacI PaeR7I
PflFI PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
SexAI SfiI SfoI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SwaI
Tth111I XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
Acc65I 972 GGTACC G^GTACC
AcyI 423 GGCGTC G[GA]^CG[CT]C
Aor51HI 1549 AGCGCT AGC^GCT
Asp718I 972 GGTACC G^GTACC
AvaI 390 CTCGGG C^[CT]CG[GA]G
BalI 1607 TGGCCA TGG^CCA
BamHI 1041 GGATCC G^GATCC
BbrPI 1568 CACGTG CAC^GTG
BbvCI 962 CCTCAGC CCTCAGC(-5/-2)
BcgI 831 GCAGATGATTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 732 CCGGG CC^[GC]GG
BlpI 1585 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1585 GCTGAGC GC^T[ACGT]AGC
BsaAI 1568 CACGTG [CT]AC^GT[GA]
BsaBI 944 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 423 GGCGTC G[GA]^CG[CT]C
BseRI 409 GAGGAG GAGGAG(10/8)
BsgI 1571 GTGCAG GTGCAG(16/14)
BsmBI 1532 GAGACG CGTCTC(1/5)
BsoBI 390 CTCGGG C^[CT]CG[GA]G
Bsp1407I 1966 TGTACA T^GTACA
BspHI 1392 TCATGA T^CATGA
BsrDI 538 GCAATG GCAATG(2/0)
BsrGI 1966 TGTACA T^GTACA
BstAPI 2297 GCACAATCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 560 CCACAGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1585 GCTGAGC GC^T[ACGT]AGC
DraIII 2640 CACTCCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1607 TGGCCA [CT]^GGCC[GA]
Eco47III 1549 AGCGCT AGC^GCT
EcoRV 1293 GATATC GAT^ATC
HaeII 1549 AGCGCT [GA]GCGC^[CT]
HapII 732 CCGG C^CGG
Hin1I 423 GGCGTC G[GA]^CG[CT]C
HindIII 2061 AAGCTT A^AGCTT
HpaII 732 CCGG C^CGG
KpnI 972 GGTACC GGTAC^C
MaeII 1569 ACGT A^CGT
MamI 944 GATCCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 1607 TGGCCA TGG^CCA
MscI 1607 TGGCCA TGG^CCA
MspI 732 CCGG C^CGG
NciI 732 CCGGG CC^[GC]GG
NheI 2916 GCTAGC G^CTAGC
PmaCI 1568 CACGTG CAC^GTG
PmlI 1568 CACGTG CAC^GTG
PpuMI 2487 AGGTCCT [GA]G^G[AT]CC[CT]
PshAI 1534 GACGCAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1392 TCATGA T^CATGA
SacI 2250 GAGCTC GAGCT^C
ScaI 1690 AGTACT AGT^ACT
SspBI 1966 TGTACA T^GTACA
SspI 1425 AATATT AAT^ATT
StuI 1518 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflII 139 CTTAAG C^TTAAG
2067 CTTAAG
AflIII 2274 ACATGT A^C[GA][CT]GT
2790 ACATGT
AocI 595 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
BanI 972 GGTACC G^G[CT][GA]CC
3198 GGCACC
BciVI 2982 GGATAC GTATCC(6/5)
3319 GTATCC
BfrI 139 CTTAAG C^TTAAG
2067 CTTAAG
BsaI 1766 GAGACC GGTCTC(1/5)
1779 GGTCTC
BsmFI 520 GGGAC GGGAC(10/14)
2218 GGGAC
BspLU11I 2274 ACATGT A^CATGT
2790 ACATGT
BstBI 617 TTCGAA TT^CGAA
1287 TTCGAA
Bsu36I 595 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
CfoI 633 GCGC GCG^C
1550 GCGC
DraI 265 TTTAAA TTT^AAA
2173 TTTAAA
DraII 1331 AGGCCCT [GA]G^G[ACGT]CC[CT]
2487 AGGTCCT
DrdI 1234 GACAGTAAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2319 GACAGCCAAGTC
Eco81I 595 CCTCAGG CC^T[ACGT]AGG
772 CCTGAGG
EcoO109I 1331 AGGCCCT [GA]G^G[ACGT]CC[CT]
2487 AGGTCCT
FauI 1308 CCCGC CCCGC(4/6)
517 GCGGG
HgaI 1534 GACGC GACGC(5/10)
424 GCGTC
HhaI 633 GCGC GCG^C
1550 GCGC
HinP1I 633 GCGC G^CGC
1550 GCGC
NspV 617 TTCGAA TT^CGAA
1287 TTCGAA
PflMI 1714 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2496 CCAACGCATGG
PstI 1273 CTGCAG CTGCA^G
2629 CTGCAG
PvuII 563 CAGCTG CAG^CTG
2111 CAGCTG
SfuI 617 TTCGAA TT^CGAA
1287 TTCGAA
Van91I 1714 CCAGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2496 CCAACGCATGG
XbaI 1378 TCTAGA T^CTAGA
2193 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaII BanII
BbsI BbvI BclI BfaI BglII
BmyI BpmI BpuAI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII