##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BamHI BbrPI BbsI BclI
BcnI BfrI BlnI BpuAI BsaAI
BsaBI BseAI BsiEI BsiWI BsmBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I Cfr10I ClaI CpoI
DraI DrdI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KspI MaeII
MamI MfeI MluI MroI MseI
MunI MvnI NaeI NciI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SspBI SspI SwaI
TfiI Tru9I Tth111I XbaI XcmI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
Acc65I 1156 GGTACC G^GTACC
AcsI 365 GAATTC [GA]^AATT[CT]
AcyI 324 GGCGCC G[GA]^CG[CT]C
ApaI 411 GGGCCC GGGCC^C
ApoI 365 GAATTC [GA]^AATT[CT]
Asp718I 1156 GGTACC G^GTACC
BalI 1075 TGGCCA TGG^CCA
BbeI 324 GGCGCC GGCGC^C
BbvCI 1247 CCTCAGC CCTCAGC(-5/-2)
BcgI 526 GCATGGCCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 209 GGATAC GTATCC(6/5)
BglI 630 GCCCTGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1369 AGATCT A^GATCT
BsaHI 324 GGCGCC G[GA]^CG[CT]C
BsgI 481 GTGCAG GTGCAG(16/14)
BsmI 37 GCATTC GAATGC(1/-1)
BsrBI 1179 CCGCTC CCGCTC(-3/-3)
BssSI 32 CTCGTG CACGAG(-5/-1)
BstXI 817 CCAGTGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 29 CACCTCGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1075 TGGCCA [CT]^GGCC[GA]
EcoNI 1235 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 365 GAATTC G^AATTC
HgaI 96 GCGTC GACGC(5/10)
Hin1I 324 GGCGCC G[GA]^CG[CT]C
KasI 324 GGCGCC G^GCGCC
KpnI 1156 GGTACC GGTAC^C
MluNI 1075 TGGCCA TGG^CCA
MscI 1075 TGGCCA TGG^CCA
MslI 564 CATAAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 324 GGCGCC GG^CGCC
NcoI 283 CCATGG C^CATGG
PaeR7I 534 CTCGAG C^TCGAG
PflMI 986 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 506 GCTCTTC GCTCTTC(1/4)
SfaNI 1252 GCATC GCATC(5/9)
SfoI 324 GGCGCC GGC^GCC
SpeI 1331 ACTAGT A^CTAGT
StuI 1258 AGGCCT AGG^CCT
Tsp509I 366 AATT ^AATT
Van91I 986 CCAGAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 534 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AfaI 225 GTAC GT^AC
1157 GTAC
AspHI 195 GAGCTC G[AT]GC[AT]^C
705 GAGCTC
AvaI 294 CCCGAG C^[CT]CG[GA]G
534 CTCGAG
BanI 324 GGCGCC G^G[CT][GA]CC
1156 GGTACC
BfaI 885 CTAG C^TAG
1332 CTAG
BlpI 192 GCTGAGC GC^T[ACGT]AGC
1172 GCTGAGC
Bpu1102I 192 GCTGAGC GC^T[ACGT]AGC
1172 GCTGAGC
BsaWI 940 TCCGGT [AT]^CCGG[AT]
1060 ACCGGA
BsiHKAI 195 GAGCTC G[AT]GC[AT]^C
705 GAGCTC
BsoBI 294 CCCGAG C^[CT]CG[GA]G
534 CTCGAG
CelII 192 GCTGAGC GC^T[ACGT]AGC
1172 GCTGAGC
DsaI 271 CCACGG C^C[GA][CT]GG
283 CCATGG
HaeII 324 GGCGCC [GA]GCGC^[CT]
454 AGCGCC
MaeI 885 CTAG C^TAG
1332 CTAG
RsaI 225 GTAC GT^AC
1157 GTAC
SacI 195 GAGCTC GAGCT^C
705 GAGCTC
SmlI 534 CTCGAG C^T[CT][GA]AG
708 CTCAAG
TaqI 535 TCGA T^CGA
1083 TCGA
TthHB8I 535 TCGA T^CGA
1083 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanII BbvI BmyI BpmI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeIII MboI MboII MflI MnlI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV PleI PstI PvuII
Sau3AI Sau96I ScrFI SfcI StyI
TseI Tsp45I TspRI XhoII