################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## AccIII AclI AflIII AgeI AhdI Aor51HI AscI AspEI AspI AviII AvrII BamHI BbeI BbrPI BbvCI BcgI BlnI BsaI BsaWI BseAI BsiEI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrFI BssHII BssSI BstBI BstEII BstPI BstXI Cfr10I ClaI CpoI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoRV FseI FspI HaeII HindIII HpaI KasI KspI MluI MroI NaeI NarI NdeI NgoMIV NotI NruI NspV PflFI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI Sse8387I Tth111I XbaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AatII 1225 GACGTC GACGT^C Acc65I 2304 GGTACC G^GTACC AlwNI 1878 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG Asp718I 2304 GGTACC G^GTACC AvaI 2053 CTCGAG C^[CT]CG[GA]G AvaII 327 GGACC G^G[AT]CC BalI 1455 TGGCCA TGG^CCA BanI 2304 GGTACC G^G[CT][GA]CC BciVI 2270 GGATAC GTATCC(6/5) BclI 2113 TGATCA T^GATCA BcnI 1933 CCCGG CC^[GC]GG BglI 731 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 2995 AGATCT A^GATCT BsaBI 1406 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsgI 3433 GTGCAG GTGCAG(16/14) BsoBI 2053 CTCGAG C^[CT]CG[GA]G BspMI 4224 GCAGGT ACCTGC(4/8) DrdI 1538 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 2113 TGATCA T^GATCA HapII 1934 CCGG C^CGG HgaI 871 GACGC GACGC(5/10) HincII 868 GTCGAC GT[CT]^[GA]AC HindII 868 GTCGAC GT[CT]^[GA]AC HpaII 1934 CCGG C^CGG KpnI 2304 GGTACC GGTAC^C MamI 1406 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 2283 CAATTG C^AATTG MluNI 1455 TGGCCA TGG^CCA MscI 1455 TGGCCA TGG^CCA MspI 1934 CCGG C^CGG MunI 2283 CAATTG C^AATTG NciI 1933 CCCGG CC^[GC]GG NheI 2068 GCTAGC G^CTAGC PacI 3152 TTAATTAA TTAAT^TAA PaeR7I 2053 CTCGAG C^TCGAG PflMI 2460 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG SalI 868 GTCGAC G^TCGAC ScaI 200 AGTACT AGT^ACT SpeI 2500 ACTAGT A^CTAGT SwaI 2824 ATTTAAAT ATTT^AAAT Van91I 2460 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoI 2053 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccII 38 CGCG CG^CG 1142 CGCG AcyI 871 GACGCC G[GA]^CG[CT]C 1225 GACGTC AflII 1664 CTTAAG C^TTAAG 2311 CTTAAG AocI 3258 CCTTAGG CC^T[ACGT]AGG 3299 CCTGAGG ApaI 3443 GGGCCC GGGCC^C 3708 GGGCCC Asp700I 656 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1103 GAAAGTCTTC BfrI 1664 CTTAAG C^TTAAG 2311 CTTAAG BlpI 272 GCTCAGC GC^T[ACGT]AGC 948 GCTCAGC Bpu1102I 272 GCTCAGC GC^T[ACGT]AGC 948 GCTCAGC BsaAI 2908 CACGTA [CT]AC^GT[GA] 4187 CACGTA BsaHI 871 GACGCC G[GA]^CG[CT]C 1225 GACGTC BsrBI 2295 CCGCTC CCGCTC(-3/-3) 943 GAGCGG BstAPI 1115 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1232 GCAATTTATGC BstUI 38 CGCG CG^CG 1142 CGCG Bsu36I 3258 CCTTAGG CC^T[ACGT]AGG 3299 CCTGAGG CelII 272 GCTCAGC GC^T[ACGT]AGC 948 GCTCAGC DraIII 630 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG 1116 CACTGTGTG EaeI 1278 CGGCCA [CT]^GGCC[GA] 1455 TGGCCA EarI 482 CTCTTC CTCTTC(1/4) 1067 CTCTTC Eco81I 3258 CCTTAGG CC^T[ACGT]AGG 3299 CCTGAGG EcoRI 660 GAATTC G^AATTC 667 GAATTC Hin1I 871 GACGCC G[GA]^CG[CT]C 1225 GACGTC Ksp632I 482 CTCTTC CTCTTC(1/4) 1067 CTCTTC MvnI 38 CGCG CG^CG 1142 CGCG NcoI 474 CCATGG C^CATGG 1458 CCATGG SacI 5 GAGCTC GAGCT^C 500 GAGCTC SphI 239 GCATGC GCATG^C 2923 GCATGC StuI 858 AGGCCT AGG^CCT 2151 AGGCCT XmnI 656 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1103 GAAAGTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 99 enzymes) # ######################################### AccI AciI AcsI AfaI AluI Alw44I AlwI ApaLI ApoI AseI AsnI AspHI BanII BbsI BbvI BfaI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EcoO109I EcoRII EcoT14I EcoT22I FauI Fnu4HI FokI HaeIII HhaI HinP1I HinfI HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MvaI MwoI NdeII NlaIII NlaIV NsiI NspI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII