##################################
# NO CUT ENZYMES (   88 enzymes) #
##################################

AccIII      AclI        AflIII      AgeI        AhdI        
Aor51HI     AscI        AspEI       AspI        AviII       
AvrII       BamHI       BbeI        BbrPI       BbvCI       
BcgI        BlnI        BsaI        BsaWI       BseAI       
BsiEI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BsrFI       BssHII      BssSI       
BstBI       BstEII      BstPI       BstXI       Cfr10I      
ClaI        CpoI        EagI        Eam1105I    EclXI       
Eco47III    Eco52I      EcoNI       EcoO65I     EcoRV       
FseI        FspI        HaeII       HindIII     HpaI        
KasI        KspI        MluI        MroI        NaeI        
NarI        NdeI        NgoMIV      NotI        NruI        
NspV        PflFI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       Psp1406I    PvuI        
RcaI        RsrII       SacII       SapI        SbfI        
SexAI       SfiI        SfoI        SfuI        SgrAI       
SmaI        SnaBI       Sse8387I    Tth111I     XbaI        
XcmI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AatII        1225  GACGTC               GACGT^C
Acc65I       2304  GGTACC               G^GTACC
AlwNI        1878  CAGATGCTG            CAG[ACGT][ACGT][ACGT]^CTG
Asp718I      2304  GGTACC               G^GTACC
AvaI         2053  CTCGAG               C^[CT]CG[GA]G
AvaII         327  GGACC                G^G[AT]CC
BalI         1455  TGGCCA               TGG^CCA
BanI         2304  GGTACC               G^G[CT][GA]CC
BciVI        2270  GGATAC               GTATCC(6/5)
BclI         2113  TGATCA               T^GATCA
BcnI         1933  CCCGG                CC^[GC]GG
BglI          731  GCCGCCGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII        2995  AGATCT               A^GATCT
BsaBI        1406  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI         3433  GTGCAG               GTGCAG(16/14)
BsoBI        2053  CTCGAG               C^[CT]CG[GA]G
BspMI        4224  GCAGGT               ACCTGC(4/8)
DrdI         1538  GACCCTGCTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI         2113  TGATCA               T^GATCA
HapII        1934  CCGG                 C^CGG
HgaI          871  GACGC                GACGC(5/10)
HincII        868  GTCGAC               GT[CT]^[GA]AC
HindII        868  GTCGAC               GT[CT]^[GA]AC
HpaII        1934  CCGG                 C^CGG
KpnI         2304  GGTACC               GGTAC^C
MamI         1406  GATTTGAATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI         2283  CAATTG               C^AATTG
MluNI        1455  TGGCCA               TGG^CCA
MscI         1455  TGGCCA               TGG^CCA
MspI         1934  CCGG                 C^CGG
MunI         2283  CAATTG               C^AATTG
NciI         1933  CCCGG                CC^[GC]GG
NheI         2068  GCTAGC               G^CTAGC
PacI         3152  TTAATTAA             TTAAT^TAA
PaeR7I       2053  CTCGAG               C^TCGAG
PflMI        2460  CCAAGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI          868  GTCGAC               G^TCGAC
ScaI          200  AGTACT               AGT^ACT
SpeI         2500  ACTAGT               A^CTAGT
SwaI         2824  ATTTAAAT             ATTT^AAAT
Van91I       2460  CCAAGTGTTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI         2053  CTCGAG               C^TCGAG


###################################
# TWO CUT ENZYMES (   29 enzymes) #
###################################

AccII          38  CGCG                 CG^CG
             1142  CGCG                
AcyI          871  GACGCC               G[GA]^CG[CT]C
             1225  GACGTC              
AflII        1664  CTTAAG               C^TTAAG
             2311  CTTAAG              
AocI         3258  CCTTAGG              CC^T[ACGT]AGG
             3299  CCTGAGG             
ApaI         3443  GGGCCC               GGGCC^C
             3708  GGGCCC              
Asp700I       656  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1103  GAAAGTCTTC          
BfrI         1664  CTTAAG               C^TTAAG
             2311  CTTAAG              
BlpI          272  GCTCAGC              GC^T[ACGT]AGC
              948  GCTCAGC             
Bpu1102I      272  GCTCAGC              GC^T[ACGT]AGC
              948  GCTCAGC             
BsaAI        2908  CACGTA               [CT]AC^GT[GA]
             4187  CACGTA              
BsaHI         871  GACGCC               G[GA]^CG[CT]C
             1225  GACGTC              
BsrBI        2295  CCGCTC               CCGCTC(-3/-3)
              943  GAGCGG              
BstAPI       1115  GCACTGTGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1232  GCAATTTATGC         
BstUI          38  CGCG                 CG^CG
             1142  CGCG                
Bsu36I       3258  CCTTAGG              CC^T[ACGT]AGG
             3299  CCTGAGG             
CelII         272  GCTCAGC              GC^T[ACGT]AGC
              948  GCTCAGC             
DraIII        630  CACAGGGTG            CAC[ACGT][ACGT][ACGT]^GTG
             1116  CACTGTGTG           
EaeI         1278  CGGCCA               [CT]^GGCC[GA]
             1455  TGGCCA              
EarI          482  CTCTTC               CTCTTC(1/4)
             1067  CTCTTC              
Eco81I       3258  CCTTAGG              CC^T[ACGT]AGG
             3299  CCTGAGG             
EcoRI         660  GAATTC               G^AATTC
              667  GAATTC              
Hin1I         871  GACGCC               G[GA]^CG[CT]C
             1225  GACGTC              
Ksp632I       482  CTCTTC               CTCTTC(1/4)
             1067  CTCTTC              
MvnI           38  CGCG                 CG^CG
             1142  CGCG                
NcoI          474  CCATGG               C^CATGG
             1458  CCATGG              
SacI            5  GAGCTC               GAGCT^C
              500  GAGCTC              
SphI          239  GCATGC               GCATG^C
             2923  GCATGC              
StuI          858  AGGCCT               AGG^CCT
             2151  AGGCCT              
XmnI          656  GAAAGAATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             1103  GAAAGTCTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   99 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
Alw44I      AlwI        ApaLI       ApoI        AseI        
AsnI        AspHI       BanII       BbsI        BbvI        
BfaI        BmyI        BpmI        BpuAI       BsaJI       
BseRI       BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsmI        Bsp1286I    Bsp1407I    BsrDI       
BsrGI       BsrI        BssKI       Bst1107I    BstF5I      
BstNI       BstYI       BstZ17I     Cac8I       CfoI        
Cfr13I      DdeI        DpnII       DraI        DraII       
DsaI        EcoO109I    EcoRII      EcoT14I     EcoT22I     
FauI        Fnu4HI      FokI        HaeIII      HhaI        
HinP1I      HinfI       HphI        ItaI        MaeI        
MaeII       MaeIII      MboI        MboII       MflI        
MnlI        MseI        MslI        MspA1I      MvaI        
MwoI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PleI        PshBI       PstI        PvuII       
RsaI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        SmlI        SspBI       SspI        StyI        
TaqI        TfiI        Tru9I       TseI        Tsp45I      
Tsp509I     TspRI       TthHB8I     XhoII