##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
AccIII AclI AflIII AgeI AhdI
Aor51HI AscI AspEI AspI AviII
AvrII BamHI BbeI BbrPI BbvCI
BcgI BlnI BsaI BsaWI BseAI
BsiEI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrFI BssHII BssSI
BstBI BstEII BstPI BstXI Cfr10I
ClaI CpoI EagI Eam1105I EclXI
Eco47III Eco52I EcoNI EcoO65I EcoRV
FseI FspI HaeII HindIII HpaI
KasI KspI MluI MroI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PflFI PinAI PmaCI PmeI
PmlI PpuMI PshAI Psp1406I PvuI
RcaI RsrII SacII SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI Sse8387I Tth111I XbaI
XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AatII 1225 GACGTC GACGT^C
Acc65I 2304 GGTACC G^GTACC
AlwNI 1878 CAGATGCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp718I 2304 GGTACC G^GTACC
AvaI 2053 CTCGAG C^[CT]CG[GA]G
AvaII 327 GGACC G^G[AT]CC
BalI 1455 TGGCCA TGG^CCA
BanI 2304 GGTACC G^G[CT][GA]CC
BciVI 2270 GGATAC GTATCC(6/5)
BclI 2113 TGATCA T^GATCA
BcnI 1933 CCCGG CC^[GC]GG
BglI 731 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 2995 AGATCT A^GATCT
BsaBI 1406 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsgI 3433 GTGCAG GTGCAG(16/14)
BsoBI 2053 CTCGAG C^[CT]CG[GA]G
BspMI 4224 GCAGGT ACCTGC(4/8)
DrdI 1538 GACCCTGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 2113 TGATCA T^GATCA
HapII 1934 CCGG C^CGG
HgaI 871 GACGC GACGC(5/10)
HincII 868 GTCGAC GT[CT]^[GA]AC
HindII 868 GTCGAC GT[CT]^[GA]AC
HpaII 1934 CCGG C^CGG
KpnI 2304 GGTACC GGTAC^C
MamI 1406 GATTTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 2283 CAATTG C^AATTG
MluNI 1455 TGGCCA TGG^CCA
MscI 1455 TGGCCA TGG^CCA
MspI 1934 CCGG C^CGG
MunI 2283 CAATTG C^AATTG
NciI 1933 CCCGG CC^[GC]GG
NheI 2068 GCTAGC G^CTAGC
PacI 3152 TTAATTAA TTAAT^TAA
PaeR7I 2053 CTCGAG C^TCGAG
PflMI 2460 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SalI 868 GTCGAC G^TCGAC
ScaI 200 AGTACT AGT^ACT
SpeI 2500 ACTAGT A^CTAGT
SwaI 2824 ATTTAAAT ATTT^AAAT
Van91I 2460 CCAAGTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2053 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccII 38 CGCG CG^CG
1142 CGCG
AcyI 871 GACGCC G[GA]^CG[CT]C
1225 GACGTC
AflII 1664 CTTAAG C^TTAAG
2311 CTTAAG
AocI 3258 CCTTAGG CC^T[ACGT]AGG
3299 CCTGAGG
ApaI 3443 GGGCCC GGGCC^C
3708 GGGCCC
Asp700I 656 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1103 GAAAGTCTTC
BfrI 1664 CTTAAG C^TTAAG
2311 CTTAAG
BlpI 272 GCTCAGC GC^T[ACGT]AGC
948 GCTCAGC
Bpu1102I 272 GCTCAGC GC^T[ACGT]AGC
948 GCTCAGC
BsaAI 2908 CACGTA [CT]AC^GT[GA]
4187 CACGTA
BsaHI 871 GACGCC G[GA]^CG[CT]C
1225 GACGTC
BsrBI 2295 CCGCTC CCGCTC(-3/-3)
943 GAGCGG
BstAPI 1115 GCACTGTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1232 GCAATTTATGC
BstUI 38 CGCG CG^CG
1142 CGCG
Bsu36I 3258 CCTTAGG CC^T[ACGT]AGG
3299 CCTGAGG
CelII 272 GCTCAGC GC^T[ACGT]AGC
948 GCTCAGC
DraIII 630 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
1116 CACTGTGTG
EaeI 1278 CGGCCA [CT]^GGCC[GA]
1455 TGGCCA
EarI 482 CTCTTC CTCTTC(1/4)
1067 CTCTTC
Eco81I 3258 CCTTAGG CC^T[ACGT]AGG
3299 CCTGAGG
EcoRI 660 GAATTC G^AATTC
667 GAATTC
Hin1I 871 GACGCC G[GA]^CG[CT]C
1225 GACGTC
Ksp632I 482 CTCTTC CTCTTC(1/4)
1067 CTCTTC
MvnI 38 CGCG CG^CG
1142 CGCG
NcoI 474 CCATGG C^CATGG
1458 CCATGG
SacI 5 GAGCTC GAGCT^C
500 GAGCTC
SphI 239 GCATGC GCATG^C
2923 GCATGC
StuI 858 AGGCCT AGG^CCT
2151 AGGCCT
XmnI 656 GAAAGAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1103 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
Alw44I AlwI ApaLI ApoI AseI
AsnI AspHI BanII BbsI BbvI
BfaI BmyI BpmI BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BsrDI
BsrGI BsrI BssKI Bst1107I BstF5I
BstNI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EcoO109I EcoRII EcoT14I EcoT22I
FauI Fnu4HI FokI HaeIII HhaI
HinP1I HinfI HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NsiI
NspI PleI PshBI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI SspI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII