##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AocI Aor51HI ApaI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BalI BamHI
BbeI BbrPI BcgI BciVI BfrI
BglI BlnI BsaBI BseAI BsiEI
BsmI BspDI BspEI BspHI BspLU11I
BsrDI BssHII Bst1107I BstXI BstZ17I
Bsu36I ClaI CpoI DraI DraII
DraIII EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I FseI FspI
HindIII KasI KspI MamI MfeI
MluI MluNI MroI MscI MunI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacII SbfI SfiI SfoI
SgrAI SnaBI SphI Sse8387I SwaI
Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Alw44I 1986 GTGCAC G^TGCAC
ApaLI 1986 GTGCAC G^TGCAC
BclI 814 TGATCA T^GATCA
BlpI 1041 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1041 GCTGAGC GC^T[ACGT]AGC
BsaAI 2354 CACGTA [CT]AC^GT[GA]
BsiWI 2356 CGTACG C^GTACG
BsmBI 2493 GAGACG CGTCTC(1/5)
Bsp1407I 3538 TGTACA T^GTACA
BspMI 2563 ACCTGC ACCTGC(4/8)
BsrBI 1271 CCGCTC CCGCTC(-3/-3)
BsrFI 397 ACCGGC [GA]^CCGG[CT]
BsrGI 3538 TGTACA T^GTACA
BstAPI 1256 GCACTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 403 TTCGAA TT^CGAA
BstEII 2983 GGTAACC G^GT[ACGT]ACC
BstPI 2983 GGTAACC G^GT[ACGT]ACC
CelII 1041 GCTGAGC GC^T[ACGT]AGC
Cfr10I 397 ACCGGC [GA]^CCGG[CT]
EcoNI 1187 CCTGGACGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2983 GGTAACC G^GT[ACGT]ACC
EcoRI 2616 GAATTC G^AATTC
EcoRV 18 GATATC GAT^ATC
EcoT22I 3679 ATGCAT ATGCA^T
FauI 1996 GCGGG CCCGC(4/6)
FbaI 814 TGATCA T^GATCA
HpaI 3350 GTTAAC GTT^AAC
NsiI 3679 ATGCAT ATGCA^T
NspI 1973 GCATGT [GA]CATG^[CT]
NspV 403 TTCGAA TT^CGAA
PflMI 1094 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 954 GACTCATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2192 GTCGAC G^TCGAC
ScaI 2442 AGTACT AGT^ACT
SexAI 24 ACCAGGT A^CC[AT]GGT
SfuI 403 TTCGAA TT^CGAA
SmaI 936 CCCGGG CCC^GGG
SpeI 2439 ACTAGT A^CTAGT
SspBI 3538 TGTACA T^GTACA
StuI 1078 AGGCCT AGG^CCT
Van91I 1094 CCAAGATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 304 CCATGTACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 936 CCCGGG C^CCGGG
XmaI 936 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
Acc65I 1201 GGTACC G^GTACC
1727 GGTACC
AccI 1838 GTCTAC GT^[AC][GT]AC
2192 GTCGAC
AccII 855 CGCG CG^CG
1804 CGCG
AcyI 254 GACGCC G[GA]^CG[CT]C
2189 GGCGTC
Asp718I 1201 GGTACC G^GTACC
1727 GGTACC
BbsI 867 GAAGAC GAAGAC(2/6)
1748 GTCTTC
BpuAI 867 GAAGAC GAAGAC(2/6)
1748 GTCTTC
BsaHI 254 GACGCC G[GA]^CG[CT]C
2189 GGCGTC
BsaWI 2601 ACCGGA [AT]^CCGG[AT]
3017 TCCGGT
BssSI 918 CACGAG CACGAG(-5/-1)
2264 CACGAG
BstUI 855 CGCG CG^CG
1804 CGCG
DrdI 1739 GACCAAACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2420 GACGATCACGTC
DsaI 1504 CCACGG C^C[GA][CT]GG
2652 CCGTGG
Hin1I 254 GACGCC G[GA]^CG[CT]C
2189 GGCGTC
KpnI 1201 GGTACC GGTAC^C
1727 GGTACC
MvnI 855 CGCG CG^CG
1804 CGCG
PstI 1480 CTGCAG CTGCA^G
2035 CTGCAG
SacI 129 GAGCTC GAGCT^C
1008 GAGCTC
SapI 673 GAAGAGC GCTCTTC(1/4)
2086 GCTCTTC
XbaI 1550 TCTAGA T^CTAGA
3147 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanI BanII BbvCI BbvI BcnI
BfaI BglII BmyI BpmI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HincII HindII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII