##################################
# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII AccII AclI AcyI AgeI
AhdI Aor51HI ApaI AscI AspEI
AvaI AviII BbeI BbrPI BbvCI
BcgI BcnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsgI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstBI BstUI BstXI BstZ17I
CelII CfoI ClaI CpoI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI FauI FseI
FspI HaeII HgaI HhaI Hin1I
HinP1I KasI KspI MamI MfeI
MluI MunI MvnI NaeI NarI
NciI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RsrII SacII SapI SbfI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SphI Sse8387I SspBI
SwaI XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 55 enzymes) #
###################################
Acc65I 655 GGTACC G^GTACC
AccI 1171 GTCGAC GT^[AC][GT]AC
AccIII 94 TCCGGA T^CCGGA
AflII 3144 CTTAAG C^TTAAG
Alw44I 1421 GTGCAC G^TGCAC
AlwNI 680 CAGAGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 1523 CCTCAGG CC^T[ACGT]AGG
ApaLI 1421 GTGCAC G^TGCAC
AseI 3584 ATTAAT AT^TAAT
AsnI 3584 ATTAAT AT^TAAT
Asp718I 655 GGTACC G^GTACC
AspI 678 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 478 CCTAGG C^CTAGG
BamHI 1828 GGATCC G^GATCC
BanII 919 GAGCTC G[GA]GC[CT]^C
BbsI 2523 GTCTTC GAAGAC(2/6)
BciVI 2749 GTATCC GTATCC(6/5)
BfrI 3144 CTTAAG C^TTAAG
BglI 368 GCCACCGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 3303 AGATCT A^GATCT
BlnI 478 CCTAGG C^CTAGG
BpuAI 2523 GTCTTC GAAGAC(2/6)
BsaAI 2308 TACGTG [CT]AC^GT[GA]
BsaWI 94 TCCGGA [AT]^CCGG[AT]
BseAI 94 TCCGGA T^CCGGA
BseRI 2947 GAGGAG GAGGAG(10/8)
BspEI 94 TCCGGA T^CCGGA
BspHI 915 TCATGA T^CATGA
BsrFI 268 ACCGGC [GA]^CCGG[CT]
BstAPI 2182 GCAATATTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 1523 CCTCAGG CC^T[ACGT]AGG
Cfr10I 268 ACCGGC [GA]^CCGG[CT]
Eco81I 1523 CCTCAGG CC^T[ACGT]AGG
EcoRV 463 GATATC GAT^ATC
EcoT22I 1551 ATGCAT ATGCA^T
HindIII 3641 AAGCTT A^AGCTT
HpaI 2574 GTTAAC GTT^AAC
KpnI 655 GGTACC GGTAC^C
MroI 94 TCCGGA T^CCGGA
MslI 1138 CACGTTGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 660 CAGCTG C[AC]G^C[GT]G
NcoI 2299 CCATGG C^CATGG
NdeI 2129 CATATG CA^TATG
NsiI 1551 ATGCAT ATGCA^T
PflFI 678 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3584 ATTAAT AT^TAAT
PstI 635 CTGCAG CTGCA^G
PvuII 660 CAGCTG CAG^CTG
RcaI 915 TCATGA T^CATGA
SacI 919 GAGCTC GAGCT^C
SalI 1171 GTCGAC G^TCGAC
SpeI 2864 ACTAGT A^CTAGT
StuI 475 AGGCCT AGG^CCT
Tth111I 678 GACAGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2753 CCACAACCATCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AciI 366 CCGC CCGC(-3/-1)
453 GCGG
AflIII 936 ACATGT A^C[GA][CT]GT
2268 ACATGT
BalI 511 TGGCCA TGG^CCA
1782 TGGCCA
BanI 655 GGTACC G^G[CT][GA]CC
3608 GGTGCC
BspLU11I 936 ACATGT A^CATGT
2268 ACATGT
BstEII 1464 GGTTACC G^GT[ACGT]ACC
2215 GGTAACC
BstPI 1464 GGTTACC G^GT[ACGT]ACC
2215 GGTAACC
DraII 165 AGGACCT [GA]G^G[ACGT]CC[CT]
648 AGGTCCT
DsaI 372 CCGTGG C^C[GA][CT]GG
2299 CCATGG
EaeI 511 TGGCCA [CT]^GGCC[GA]
1782 TGGCCA
EarI 432 GAAGAG CTCTTC(1/4)
1164 GAAGAG
EcoO109I 165 AGGACCT [GA]G^G[ACGT]CC[CT]
648 AGGTCCT
EcoO65I 1464 GGTTACC G^GT[ACGT]ACC
2215 GGTAACC
HapII 95 CCGG C^CGG
269 CCGG
HpaII 95 CCGG C^CGG
269 CCGG
Ksp632I 432 GAAGAG CTCTTC(1/4)
1164 GAAGAG
MluNI 511 TGGCCA TGG^CCA
1782 TGGCCA
MscI 511 TGGCCA TGG^CCA
1782 TGGCCA
MspI 95 CCGG C^CGG
269 CCGG
PflMI 816 CCATAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCAGGACTTGG
PpuMI 165 AGGACCT [GA]G^G[AT]CC[CT]
648 AGGTCCT
SmlI 1512 CTTGAG C^T[CT][GA]AG
3144 CTTAAG
TaqI 407 TCGA T^CGA
1172 TCGA
TthHB8I 407 TCGA T^CGA
1172 TCGA
Van91I 816 CCATAATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
888 CCAGGACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Asp700I AspHI AvaII BbvI BclI
BfaI BmyI BpmI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrDI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraI EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HincII HindII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XhoII XmnI