##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AccIII AclI AgeI AscI
AseI AsnI Asp700I AspI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BsaAI BsaBI BseAI BsiWI
BspDI BspEI BspMI Bst1107I BstAPI
BstBI BstZ17I ClaI CpoI EcoRI
EcoT22I FbaI FseI HpaI KasI
KspI MamI MfeI MluI MroI
MunI NarI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SacII
SalI SbfI ScaI SexAI SfoI
SfuI SgrAI SnaBI SphI Sse8387I
SspI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
Acc65I 1728 GGTACC G^GTACC
AcsI 2538 AAATTT [GA]^AATT[CT]
AflII 3105 CTTAAG C^TTAAG
AhdI 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 467 GTGCAC G^TGCAC
ApaI 3221 GGGCCC GGGCC^C
ApaLI 467 GTGCAC G^TGCAC
ApoI 2538 AAATTT [GA]^AATT[CT]
Asp718I 1728 GGTACC G^GTACC
AspEI 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1212 TGCGCA TGC^GCA
AvrII 3475 CCTAGG C^CTAGG
BfrI 3105 CTTAAG C^TTAAG
BglI 797 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1898 AGATCT A^GATCT
BlnI 3475 CCTAGG C^CTAGG
BlpI 3179 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3179 GCTAAGC GC^T[ACGT]AGC
BsaWI 1590 ACCGGA [AT]^CCGG[AT]
BseRI 1915 GAGGAG GAGGAG(10/8)
BsmI 2520 GAATGC GAATGC(1/-1)
Bsp1407I 1292 TGTACA T^GTACA
BspLU11I 2578 ACATGT A^CATGT
BsrFI 187 GCCGGC [GA]^CCGG[CT]
BsrGI 1292 TGTACA T^GTACA
BssHII 11 GCGCGC G^CGCGC
BssSI 944 CTCGTG CACGAG(-5/-1)
CelII 3179 GCTAAGC GC^T[ACGT]AGC
Cfr10I 187 GCCGGC [GA]^CCGG[CT]
DraI 2533 TTTAAA TTT^AAA
DrdI 1982 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 189 CGGCCG C^GGCCG
Eam1105I 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 189 CGGCCG C^GGCCG
Eco52I 189 CGGCCG C^GGCCG
FspI 1212 TGCGCA TGC^GCA
HincII 317 GTCAAC GT[CT]^[GA]AC
HindII 317 GTCAAC GT[CT]^[GA]AC
KpnI 1728 GGTACC GGTAC^C
NaeI 187 GCCGGC GCC^GGC
NcoI 759 CCATGG C^CATGG
NgoMIV 187 GCCGGC G^CCGGC
PvuII 1149 CAGCTG CAG^CTG
SacI 1584 GAGCTC GAGCT^C
SfiI 796 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 223 ACTAGT A^CTAGT
SspBI 1292 TGTACA T^GTACA
StuI 2235 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AatII 2100 GACGTC GACGT^C
3323 GACGTC
AccII 12 CGCG CG^CG
160 CGCG
AcyI 2100 GACGTC G[GA]^CG[CT]C
3323 GACGTC
AflIII 2578 ACATGT A^C[GA][CT]GT
2920 ACGTGT
AlwNI 1710 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG
1865 CAGATCCTG
AocI 864 CCTCAGG CC^T[ACGT]AGG
2105 CCTCAGG
Aor51HI 167 AGCGCT AGC^GCT
2754 AGCGCT
BsaHI 2100 GACGTC G[GA]^CG[CT]C
3323 GACGTC
BsiEI 102 CGACCG CG[GA][CT]^CG
189 CGGCCG
BsmBI 1883 CGTCTC CGTCTC(1/5)
1578 GAGACG
BspHI 1735 TCATGA T^CATGA
3147 TCATGA
BstEII 1517 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
BstPI 1517 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
BstUI 12 CGCG CG^CG
160 CGCG
BstXI 1129 CCAGTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1968 CCACTACCCTGG
Bsu36I 864 CCTCAGG CC^T[ACGT]AGG
2105 CCTCAGG
Eco47III 167 AGCGCT AGC^GCT
2754 AGCGCT
Eco81I 864 CCTCAGG CC^T[ACGT]AGG
2105 CCTCAGG
EcoO65I 1517 GGTGACC G^GT[ACGT]ACC
2296 GGTGACC
EcoRV 773 GATATC GAT^ATC
1739 GATATC
Hin1I 2100 GACGTC G[GA]^CG[CT]C
3323 GACGTC
MvnI 12 CGCG CG^CG
160 CGCG
PpuMI 1980 AGGACCC [GA]G^G[AT]CC[CT]
2090 AGGACCC
PshAI 1147 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1714 GACTGCTGTC
PstI 132 CTGCAG CTGCA^G
1668 CTGCAG
RcaI 1735 TCATGA T^CATGA
3147 TCATGA
SfcI 132 CTGCAG C^T[GA][CT]AG
1668 CTGCAG
SmaI 118 CCCGGG CCC^GGG
1378 CCCGGG
SmlI 2005 CTTGAG C^T[CT][GA]AG
3105 CTTAAG
TaqI 101 TCGA T^CGA
2679 TCGA
TthHB8I 101 TCGA T^CGA
2679 TCGA
XmaCI 118 CCCGGG C^CCGGG
1378 CCCGGG
XmaI 118 CCCGGG C^CCGGG
1378 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 101 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrBI BsrDI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XcmI
XhoII