################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccI AccIII AclI AgeI AscI AseI AsnI Asp700I AspI BamHI BbeI BbrPI BbvCI BcgI BciVI BclI BsaAI BsaBI BseAI BsiWI BspDI BspEI BspMI Bst1107I BstAPI BstBI BstZ17I ClaI CpoI EcoRI EcoT22I FbaI FseI HpaI KasI KspI MamI MfeI MluI MroI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### Acc65I 1728 GGTACC G^GTACC AcsI 2538 AAATTT [GA]^AATT[CT] AflII 3105 CTTAAG C^TTAAG AhdI 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 467 GTGCAC G^TGCAC ApaI 3221 GGGCCC GGGCC^C ApaLI 467 GTGCAC G^TGCAC ApoI 2538 AAATTT [GA]^AATT[CT] Asp718I 1728 GGTACC G^GTACC AspEI 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1212 TGCGCA TGC^GCA AvrII 3475 CCTAGG C^CTAGG BfrI 3105 CTTAAG C^TTAAG BglI 797 GCCAAGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1898 AGATCT A^GATCT BlnI 3475 CCTAGG C^CTAGG BlpI 3179 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3179 GCTAAGC GC^T[ACGT]AGC BsaWI 1590 ACCGGA [AT]^CCGG[AT] BseRI 1915 GAGGAG GAGGAG(10/8) BsmI 2520 GAATGC GAATGC(1/-1) Bsp1407I 1292 TGTACA T^GTACA BspLU11I 2578 ACATGT A^CATGT BsrFI 187 GCCGGC [GA]^CCGG[CT] BsrGI 1292 TGTACA T^GTACA BssHII 11 GCGCGC G^CGCGC BssSI 944 CTCGTG CACGAG(-5/-1) CelII 3179 GCTAAGC GC^T[ACGT]AGC Cfr10I 187 GCCGGC [GA]^CCGG[CT] DraI 2533 TTTAAA TTT^AAA DrdI 1982 GACCCCAGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 189 CGGCCG C^GGCCG Eam1105I 2299 GACCAGAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 189 CGGCCG C^GGCCG Eco52I 189 CGGCCG C^GGCCG FspI 1212 TGCGCA TGC^GCA HincII 317 GTCAAC GT[CT]^[GA]AC HindII 317 GTCAAC GT[CT]^[GA]AC KpnI 1728 GGTACC GGTAC^C NaeI 187 GCCGGC GCC^GGC NcoI 759 CCATGG C^CATGG NgoMIV 187 GCCGGC G^CCGGC PvuII 1149 CAGCTG CAG^CTG SacI 1584 GAGCTC GAGCT^C SfiI 796 GGCCAAGGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SpeI 223 ACTAGT A^CTAGT SspBI 1292 TGTACA T^GTACA StuI 2235 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AatII 2100 GACGTC GACGT^C 3323 GACGTC AccII 12 CGCG CG^CG 160 CGCG AcyI 2100 GACGTC G[GA]^CG[CT]C 3323 GACGTC AflIII 2578 ACATGT A^C[GA][CT]GT 2920 ACGTGT AlwNI 1710 CAGAGACTG CAG[ACGT][ACGT][ACGT]^CTG 1865 CAGATCCTG AocI 864 CCTCAGG CC^T[ACGT]AGG 2105 CCTCAGG Aor51HI 167 AGCGCT AGC^GCT 2754 AGCGCT BsaHI 2100 GACGTC G[GA]^CG[CT]C 3323 GACGTC BsiEI 102 CGACCG CG[GA][CT]^CG 189 CGGCCG BsmBI 1883 CGTCTC CGTCTC(1/5) 1578 GAGACG BspHI 1735 TCATGA T^CATGA 3147 TCATGA BstEII 1517 GGTGACC G^GT[ACGT]ACC 2296 GGTGACC BstPI 1517 GGTGACC G^GT[ACGT]ACC 2296 GGTGACC BstUI 12 CGCG CG^CG 160 CGCG BstXI 1129 CCAGTTCTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1968 CCACTACCCTGG Bsu36I 864 CCTCAGG CC^T[ACGT]AGG 2105 CCTCAGG Eco47III 167 AGCGCT AGC^GCT 2754 AGCGCT Eco81I 864 CCTCAGG CC^T[ACGT]AGG 2105 CCTCAGG EcoO65I 1517 GGTGACC G^GT[ACGT]ACC 2296 GGTGACC EcoRV 773 GATATC GAT^ATC 1739 GATATC Hin1I 2100 GACGTC G[GA]^CG[CT]C 3323 GACGTC MvnI 12 CGCG CG^CG 160 CGCG PpuMI 1980 AGGACCC [GA]G^G[AT]CC[CT] 2090 AGGACCC PshAI 1147 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 1714 GACTGCTGTC PstI 132 CTGCAG CTGCA^G 1668 CTGCAG RcaI 1735 TCATGA T^CATGA 3147 TCATGA SfcI 132 CTGCAG C^T[GA][CT]AG 1668 CTGCAG SmaI 118 CCCGGG CCC^GGG 1378 CCCGGG SmlI 2005 CTTGAG C^T[CT][GA]AG 3105 CTTAAG TaqI 101 TCGA T^CGA 2679 TCGA TthHB8I 101 TCGA T^CGA 2679 TCGA XmaCI 118 CCCGGG C^CCGGG 1378 CCCGGG XmaI 118 CCCGGG C^CCGGG 1378 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 101 enzymes) # ######################################### AciI AfaI AluI AlwI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrDI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII