################################## # NO CUT ENZYMES ( 73 enzymes) # ################################## AatII Acc65I AccI AclI AcsI AflII AgeI AhdI ApoI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BbeI BciVI BfrI BlnI BsaBI BsmBI BspDI Bst1107I BstBI BstZ17I ClaI CpoI DrdI Eam1105I EcoRI EcoRV EcoT22I FseI FspI HpaI KasI KpnI KspI MamI MfeI MunI NarI NdeI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI ScaI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SphI SspI SwaI XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### Alw44I 1739 GTGCAC G^TGCAC Aor51HI 1662 AGCGCT AGC^GCT ApaLI 1739 GTGCAC G^TGCAC AspI 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 518 CACGTG CAC^GTG BglII 1752 AGATCT A^GATCT BsaI 1682 GAGACC GGTCTC(1/5) BsiWI 772 CGTACG C^GTACG BsmI 1802 GAATGC GAATGC(1/-1) Bsp1407I 2597 TGTACA T^GTACA BspHI 255 TCATGA T^CATGA BspLU11I 2267 ACATGT A^CATGT BsrGI 2597 TGTACA T^GTACA BssSI 581 CTCGTG CACGAG(-5/-1) DraI 377 TTTAAA TTT^AAA EagI 1192 CGGCCG C^GGCCG EclXI 1192 CGGCCG C^GGCCG Eco47III 1662 AGCGCT AGC^GCT Eco52I 1192 CGGCCG C^GGCCG EcoNI 2101 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG HincII 1426 GTTGAC GT[CT]^[GA]AC HindII 1426 GTTGAC GT[CT]^[GA]AC HindIII 74 AAGCTT A^AGCTT MluI 798 ACGCGT A^CGCGT NaeI 2384 GCCGGC GCC^GGC NgoMIV 2384 GCCGGC G^CCGGC NheI 22 GCTAGC G^CTAGC NotI 1191 GCGGCCGC GC^GGCCGC PflFI 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 518 CACGTG CAC^GTG PmlI 518 CACGTG CAC^GTG PpuMI 2109 AGGACCT [GA]G^G[AT]CC[CT] PshAI 2744 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 255 TCATGA T^CATGA SacI 977 GAGCTC GAGCT^C SapI 2523 GCTCTTC GCTCTTC(1/4) SexAI 957 ACCTGGT A^CC[AT]GGT SmaI 666 CCCGGG CCC^GGG SspBI 2597 TGTACA T^GTACA StuI 1086 AGGCCT AGG^CCT TfiI 2503 GAATC G^A[AT]TC Tth111I 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2170 CCAAGTCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 666 CCCGGG C^CCGGG XmaI 666 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 43 enzymes) # ################################### AccIII 1207 TCCGGA T^CCGGA 1575 TCCGGA AcyI 184 GACGCC G[GA]^CG[CT]C 251 GGCGTC AflIII 798 ACGCGT A^C[GA][CT]GT 2267 ACATGT AocI 446 CCTAAGG CC^T[ACGT]AGG 2508 CCTCAGG BanI 1135 GGTGCC G^G[CT][GA]CC 2196 GGCACC BbsI 2120 GAAGAC GAAGAC(2/6) 2485 GTCTTC BbvCI 2333 GCTGAGG CCTCAGC(-5/-2) 2347 GCTGAGG BcgI 2090 CGACTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1296 GCAACCGCATCG BglI 476 GCCTACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2046 GCCGAAGAGGC BlpI 76 GCTTAGC GC^T[ACGT]AGC 1072 GCTCAGC Bpu1102I 76 GCTTAGC GC^T[ACGT]AGC 1072 GCTCAGC BpuAI 2120 GAAGAC GAAGAC(2/6) 2485 GTCTTC BsaAI 518 CACGTG [CT]AC^GT[GA] 845 TACGTG BsaHI 184 GACGCC G[GA]^CG[CT]C 251 GGCGTC BsaWI 1207 TCCGGA [AT]^CCGG[AT] 1575 TCCGGA BseAI 1207 TCCGGA T^CCGGA 1575 TCCGGA BseRI 361 CTCCTC GAGGAG(10/8) 710 GAGGAG BsiEI 1192 CGGCCG CG[GA][CT]^CG 2497 CGGTCG BspEI 1207 TCCGGA T^CCGGA 1575 TCCGGA BspMI 1925 ACCTGC ACCTGC(4/8) 301 GCAGGT BsrBI 1394 GAGCGG CCGCTC(-3/-3) 1579 GAGCGG BsrDI 1101 GCAATG GCAATG(2/0) 2839 GCAATG BsrFI 1503 ACCGGC [GA]^CCGG[CT] 2384 GCCGGC BssHII 941 GCGCGC G^CGCGC 1017 GCGCGC BstAPI 850 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1000 GCAGGCAGTGC BstEII 200 GGTAACC G^GT[ACGT]ACC 1837 GGTGACC BstPI 200 GGTAACC G^GT[ACGT]ACC 1837 GGTGACC BstXI 489 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1227 CCAGCGCCCTGG Bsu36I 446 CCTAAGG CC^T[ACGT]AGG 2508 CCTCAGG CelII 76 GCTTAGC GC^T[ACGT]AGC 1072 GCTCAGC Cfr10I 1503 ACCGGC [GA]^CCGG[CT] 2384 GCCGGC DraIII 163 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG 344 CACAAAGTG EarI 2524 CTCTTC CTCTTC(1/4) 2049 GAAGAG Eco81I 446 CCTAAGG CC^T[ACGT]AGG 2508 CCTCAGG EcoO65I 200 GGTAACC G^GT[ACGT]ACC 1837 GGTGACC Hin1I 184 GACGCC G[GA]^CG[CT]C 251 GGCGTC Ksp632I 2524 CTCTTC CTCTTC(1/4) 2049 GAAGAG MroI 1207 TCCGGA T^CCGGA 1575 TCCGGA NspI 681 ACATGC [GA]CATG^[CT] 2267 ACATGT PflMI 331 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCAACCAGTGG SbfI 298 CCTGCAGG CCTGCA^GG 928 CCTGCAGG Sse8387I 298 CCTGCAGG CCTGCA^GG 928 CCTGCAGG Van91I 331 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1257 CCAACCAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI ApaI AspHI AvaI AvaII BalI BanII BbvI BclI BcnI BfaI BmyI BpmI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII