##################################
# NO CUT ENZYMES ( 73 enzymes) #
##################################
AatII Acc65I AccI AclI AcsI
AflII AgeI AhdI ApoI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BbeI BciVI
BfrI BlnI BsaBI BsmBI BspDI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI Eam1105I EcoRI EcoRV EcoT22I
FseI FspI HpaI KasI KpnI
KspI MamI MfeI MunI NarI
NdeI NruI NsiI NspV PacI
PaeR7I PinAI PmeI PshBI Psp1406I
PvuI RsrII SacII SalI ScaI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SphI SspI SwaI
XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
Alw44I 1739 GTGCAC G^TGCAC
Aor51HI 1662 AGCGCT AGC^GCT
ApaLI 1739 GTGCAC G^TGCAC
AspI 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 518 CACGTG CAC^GTG
BglII 1752 AGATCT A^GATCT
BsaI 1682 GAGACC GGTCTC(1/5)
BsiWI 772 CGTACG C^GTACG
BsmI 1802 GAATGC GAATGC(1/-1)
Bsp1407I 2597 TGTACA T^GTACA
BspHI 255 TCATGA T^CATGA
BspLU11I 2267 ACATGT A^CATGT
BsrGI 2597 TGTACA T^GTACA
BssSI 581 CTCGTG CACGAG(-5/-1)
DraI 377 TTTAAA TTT^AAA
EagI 1192 CGGCCG C^GGCCG
EclXI 1192 CGGCCG C^GGCCG
Eco47III 1662 AGCGCT AGC^GCT
Eco52I 1192 CGGCCG C^GGCCG
EcoNI 2101 CCTTGTAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HincII 1426 GTTGAC GT[CT]^[GA]AC
HindII 1426 GTTGAC GT[CT]^[GA]AC
HindIII 74 AAGCTT A^AGCTT
MluI 798 ACGCGT A^CGCGT
NaeI 2384 GCCGGC GCC^GGC
NgoMIV 2384 GCCGGC G^CCGGC
NheI 22 GCTAGC G^CTAGC
NotI 1191 GCGGCCGC GC^GGCCGC
PflFI 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 518 CACGTG CAC^GTG
PmlI 518 CACGTG CAC^GTG
PpuMI 2109 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 2744 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 255 TCATGA T^CATGA
SacI 977 GAGCTC GAGCT^C
SapI 2523 GCTCTTC GCTCTTC(1/4)
SexAI 957 ACCTGGT A^CC[AT]GGT
SmaI 666 CCCGGG CCC^GGG
SspBI 2597 TGTACA T^GTACA
StuI 1086 AGGCCT AGG^CCT
TfiI 2503 GAATC G^A[AT]TC
Tth111I 809 GACTGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2170 CCAAGTCTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 666 CCCGGG C^CCGGG
XmaI 666 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 43 enzymes) #
###################################
AccIII 1207 TCCGGA T^CCGGA
1575 TCCGGA
AcyI 184 GACGCC G[GA]^CG[CT]C
251 GGCGTC
AflIII 798 ACGCGT A^C[GA][CT]GT
2267 ACATGT
AocI 446 CCTAAGG CC^T[ACGT]AGG
2508 CCTCAGG
BanI 1135 GGTGCC G^G[CT][GA]CC
2196 GGCACC
BbsI 2120 GAAGAC GAAGAC(2/6)
2485 GTCTTC
BbvCI 2333 GCTGAGG CCTCAGC(-5/-2)
2347 GCTGAGG
BcgI 2090 CGACTGGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1296 GCAACCGCATCG
BglI 476 GCCTACACGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2046 GCCGAAGAGGC
BlpI 76 GCTTAGC GC^T[ACGT]AGC
1072 GCTCAGC
Bpu1102I 76 GCTTAGC GC^T[ACGT]AGC
1072 GCTCAGC
BpuAI 2120 GAAGAC GAAGAC(2/6)
2485 GTCTTC
BsaAI 518 CACGTG [CT]AC^GT[GA]
845 TACGTG
BsaHI 184 GACGCC G[GA]^CG[CT]C
251 GGCGTC
BsaWI 1207 TCCGGA [AT]^CCGG[AT]
1575 TCCGGA
BseAI 1207 TCCGGA T^CCGGA
1575 TCCGGA
BseRI 361 CTCCTC GAGGAG(10/8)
710 GAGGAG
BsiEI 1192 CGGCCG CG[GA][CT]^CG
2497 CGGTCG
BspEI 1207 TCCGGA T^CCGGA
1575 TCCGGA
BspMI 1925 ACCTGC ACCTGC(4/8)
301 GCAGGT
BsrBI 1394 GAGCGG CCGCTC(-3/-3)
1579 GAGCGG
BsrDI 1101 GCAATG GCAATG(2/0)
2839 GCAATG
BsrFI 1503 ACCGGC [GA]^CCGG[CT]
2384 GCCGGC
BssHII 941 GCGCGC G^CGCGC
1017 GCGCGC
BstAPI 850 GCAGGCACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1000 GCAGGCAGTGC
BstEII 200 GGTAACC G^GT[ACGT]ACC
1837 GGTGACC
BstPI 200 GGTAACC G^GT[ACGT]ACC
1837 GGTGACC
BstXI 489 CCATCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1227 CCAGCGCCCTGG
Bsu36I 446 CCTAAGG CC^T[ACGT]AGG
2508 CCTCAGG
CelII 76 GCTTAGC GC^T[ACGT]AGC
1072 GCTCAGC
Cfr10I 1503 ACCGGC [GA]^CCGG[CT]
2384 GCCGGC
DraIII 163 CACGGAGTG CAC[ACGT][ACGT][ACGT]^GTG
344 CACAAAGTG
EarI 2524 CTCTTC CTCTTC(1/4)
2049 GAAGAG
Eco81I 446 CCTAAGG CC^T[ACGT]AGG
2508 CCTCAGG
EcoO65I 200 GGTAACC G^GT[ACGT]ACC
1837 GGTGACC
Hin1I 184 GACGCC G[GA]^CG[CT]C
251 GGCGTC
Ksp632I 2524 CTCTTC CTCTTC(1/4)
2049 GAAGAG
MroI 1207 TCCGGA T^CCGGA
1575 TCCGGA
NspI 681 ACATGC [GA]CATG^[CT]
2267 ACATGT
PflMI 331 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCAACCAGTGG
SbfI 298 CCTGCAGG CCTGCA^GG
928 CCTGCAGG
Sse8387I 298 CCTGCAGG CCTGCA^GG
928 CCTGCAGG
Van91I 331 CCAATTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1257 CCAACCAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AvaI AvaII
BalI BanII BbvI BclI BcnI
BfaI BmyI BpmI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII