##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AccIII AclI AflII AgeI AscI
AseI AsnI Asp700I BamHI BbeI
BciVI BfrI BglI BsaWI BseAI
BsiWI BspDI BspEI BsrFI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DrdI
EcoO65I EcoRV EcoT22I FseI HpaI
KasI MfeI MluI MroI MunI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RsrII
SalI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI StuI
SwaI XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 272 GACGTC GACGT^C
Acc65I 1471 GGTACC G^GTACC
AccI 1342 GTCTAC GT^[AC][GT]AC
AccII 51 CGCG CG^CG
AcsI 923 GAATTC [GA]^AATT[CT]
AocI 519 CCTGAGG CC^T[ACGT]AGG
ApoI 923 GAATTC [GA]^AATT[CT]
Asp718I 1471 GGTACC G^GTACC
AspI 3213 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1900 CCTAGG C^CTAGG
BglII 1483 AGATCT A^GATCT
BlnI 1900 CCTAGG C^CTAGG
BsaBI 133 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 1015 TGTACA T^GTACA
BspHI 1648 TCATGA T^CATGA
BspLU11I 3422 ACATGT A^CATGT
BsrGI 1015 TGTACA T^GTACA
BssSI 467 CTCGTG CACGAG(-5/-1)
BstUI 51 CGCG CG^CG
Bsu36I 519 CCTGAGG CC^T[ACGT]AGG
DraIII 1392 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 519 CCTGAGG CC^T[ACGT]AGG
EcoRI 923 GAATTC G^AATTC
HincII 3788 GTCAAC GT[CT]^[GA]AC
HindII 3788 GTCAAC GT[CT]^[GA]AC
HindIII 3399 AAGCTT A^AGCTT
KpnI 1471 GGTACC GGTAC^C
KspI 50 CCGCGG CCGC^GG
MamI 133 GATGTCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 51 CGCG CG^CG
NcoI 3263 CCATGG C^CATGG
NdeI 3490 CATATG CA^TATG
NheI 715 GCTAGC G^CTAGC
PflFI 3213 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 2385 CGATCG CGAT^CG
RcaI 1648 TCATGA T^CATGA
SacII 50 CCGCGG CCGC^GG
SbfI 3273 CCTGCAGG CCTGCA^GG
SmaI 85 CCCGGG CCC^GGG
Sse8387I 3273 CCTGCAGG CCTGCA^GG
SspBI 1015 TGTACA T^GTACA
SspI 201 AATATT AAT^ATT
Tth111I 3213 GACTCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 85 CCCGGG C^CCGGG
XmaI 85 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AflIII 587 ACGTGT A^C[GA][CT]GT
3422 ACATGT
AhdI 254 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCCGCTGTC
AspEI 254 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCCGCTGTC
AviII 2093 TGCGCA TGC^GCA
2993 TGCGCA
BalI 596 TGGCCA TGG^CCA
3260 TGGCCA
BbrPI 317 CACGTG CAC^GTG
586 CACGTG
BbsI 3924 GAAGAC GAAGAC(2/6)
1730 GTCTTC
BcgI 2180 GCAAGTGGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2996 GCAGCTTCATCG
BclI 815 TGATCA T^GATCA
1032 TGATCA
BpuAI 3924 GAAGAC GAAGAC(2/6)
1730 GTCTTC
BsaI 1008 GGTCTC GGTCTC(1/5)
2234 GGTCTC
BsmI 1138 GAATGC GAATGC(1/-1)
2490 GAATGC
BsrBI 2106 CCGCTC CCGCTC(-3/-3)
2146 GAGCGG
DraI 3658 TTTAAA TTT^AAA
3667 TTTAAA
Eam1105I 254 GACCTGCGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
626 GACCCGCTGTC
FbaI 815 TGATCA T^GATCA
1032 TGATCA
FspI 2093 TGCGCA TGC^GCA
2993 TGCGCA
MluNI 596 TGGCCA TGG^CCA
3260 TGGCCA
MscI 596 TGGCCA TGG^CCA
3260 TGGCCA
PmaCI 317 CACGTG CAC^GTG
586 CACGTG
PmlI 317 CACGTG CAC^GTG
586 CACGTG
SfaNI 372 GCATC GCATC(5/9)
1637 GCATC
SmlI 696 CTTGAG C^T[CT][GA]AG
3698 CTCAAG
SphI 1400 GCATGC GCATG^C
2090 GCATGC
XcmI 292 CCAGCGCAACTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1457 CCATGCCAGTGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AciI AcyI AfaI AluI Alw44I
AlwI AlwNI Aor51HI ApaI ApaLI
AspHI AvaI AvaII BanI BanII
BbvCI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BsaAI BsaHI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI SapI Sau3AI
Sau96I ScrFI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoII