##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AflIII AscI AseI AsnI Asp718I
BbeI BfrI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrGI BssHII Bst1107I
BstBI BstUI BstZ17I ClaI CpoI
EagI EclXI Eco52I EcoRV FseI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NarI NcoI NotI NruI NspV
PacI PmeI PshBI PvuI RcaI
RsrII SacII SalI SfoI SfuI
SgrAI SnaBI SspBI SspI SwaI
XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AclI 4336 AACGTT AA^CGTT
AcsI 961 GAATTC [GA]^AATT[CT]
AgeI 258 ACCGGT A^CCGGT
AhdI 1792 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 302 GTGCAC G^TGCAC
Aor51HI 3798 AGCGCT AGC^GCT
ApaLI 302 GTGCAC G^TGCAC
ApoI 961 GAATTC [GA]^AATT[CT]
Asp700I 4258 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1792 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1544 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 181 TGCGCA TGC^GCA
AvrII 2332 CCTAGG C^CTAGG
BcgI 5063 CGACTAGGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2332 CCTAGG C^CTAGG
BsaBI 1411 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsrDI 709 CATTGC GCAATG(2/0)
BstEII 3315 GGTAACC G^GT[ACGT]ACC
BstPI 3315 GGTAACC G^GT[ACGT]ACC
DraI 3701 TTTAAA TTT^AAA
DraIII 4275 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1604 GACACCGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1792 GACCGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3798 AGCGCT AGC^GCT
EcoO65I 3315 GGTAACC G^GT[ACGT]ACC
EcoRI 961 GAATTC G^AATTC
EcoT22I 5071 ATGCAT ATGCA^T
FspI 181 TGCGCA TGC^GCA
HincII 1789 GTTGAC GT[CT]^[GA]AC
HindII 1789 GTTGAC GT[CT]^[GA]AC
MamI 1411 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 872 GCCGGC GCC^GGC
NdeI 3344 CATATG CA^TATG
NgoMIV 872 GCCGGC G^CCGGC
NsiI 5071 ATGCAT ATGCA^T
PflFI 1544 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 258 ACCGGT A^CCGGT
PshAI 1862 GACCCTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 4336 AACGTT AA^CGTT
SacI 2183 GAGCTC GAGCT^C
SapI 1298 GAAGAGC GCTCTTC(1/4)
SbfI 1204 CCTGCAGG CCTGCA^GG
ScaI 2785 AGTACT AGT^ACT
SexAI 879 ACCTGGT A^CC[AT]GGT
SfiI 2726 GGCCTCCTGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 111 CCCGGG CCC^GGG
SpeI 2426 ACTAGT A^CTAGT
Sse8387I 1204 CCTGCAGG CCTGCA^GG
Tth111I 1544 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 111 CCCGGG C^CCGGG
XmaI 111 CCCGGG C^CCGGG
XmnI 4258 GAATGTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccI 1550 GTCTAC GT^[AC][GT]AC
3776 GTAGAC
AcyI 1346 GACGCC G[GA]^CG[CT]C
3742 GACGCC
BbrPI 104 CACGTG CAC^GTG
2549 CACGTG
BciVI 3428 GGATAC GTATCC(6/5)
4063 GGATAC
BclI 3948 TGATCA T^GATCA
4083 TGATCA
BlpI 1819 GCTCAGC GC^T[ACGT]AGC
4752 GCTCAGC
Bpu1102I 1819 GCTCAGC GC^T[ACGT]AGC
4752 GCTCAGC
BsaHI 1346 GACGCC G[GA]^CG[CT]C
3742 GACGCC
BsaWI 258 ACCGGT [AT]^CCGG[AT]
4564 TCCGGT
BsrBI 1817 CCGCTC CCGCTC(-3/-3)
2394 GAGCGG
BsrFI 258 ACCGGT [GA]^CCGG[CT]
872 GCCGGC
BssSI 244 CACGAG CACGAG(-5/-1)
425 CTCGTG
BstAPI 184 GCAACTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
739 GCAACTGGTGC
CelII 1819 GCTCAGC GC^T[ACGT]AGC
4752 GCTCAGC
Cfr10I 258 ACCGGT [GA]^CCGG[CT]
872 GCCGGC
FbaI 3948 TGATCA T^GATCA
4083 TGATCA
Hin1I 1346 GACGCC G[GA]^CG[CT]C
3742 GACGCC
NheI 1360 GCTAGC G^CTAGC
2056 GCTAGC
PflMI 1393 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2163 CCAGCGGCTGG
PmaCI 104 CACGTG CAC^GTG
2549 CACGTG
PmlI 104 CACGTG CAC^GTG
2549 CACGTG
SphI 2522 GCATGC GCATG^C
4979 GCATGC
Van91I 1393 CCAGCCCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2163 CCAGCGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 117 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI ApaI AspHI AvaI AvaII
BalI BamHI BanI BanII BbsI
BbvCI BbvI BcnI BfaI BglI
BglII BmyI BpmI BpuAI BsaAI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PaeR7I PleI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoI XhoII