##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
ApaI AscI Asp718I AspI AviII
AvrII BbrPI BfrI BglI BlnI
BpmI BsaAI BsiWI BspDI BspHI
BspMI BsrBI BsrFI BssHII BssSI
BstBI Cfr10I ClaI CpoI DraIII
DrdI EcoT22I FseI FspI HgaI
HindIII KpnI KspI MfeI MluI
MunI NaeI NgoMIV NsiI NspV
PacI PflFI PinAI PmaCI PmeI
PmlI Psp1406I PvuI RcaI RsrII
SacII SapI SbfI SexAI SfiI
SfuI SgrAI SmaI SnaBI Sse8387I
SwaI Tth111I XcmI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccIII 2932 TCCGGA T^CCGGA
AhdI 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3913 GTGCAC G^TGCAC
AlwNI 4048 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 265 CCTGAGG CC^T[ACGT]AGG
Aor51HI 208 AGCGCT AGC^GCT
ApaLI 3913 GTGCAC G^TGCAC
AspEI 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 3840 TGGCCA TGG^CCA
BamHI 1660 GGATCC G^GATCC
BanII 4504 GAGCTC G[GA]GC[CT]^C
BbvCI 863 GCTGAGG CCTCAGC(-5/-2)
BcgI 1116 GCAGTTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3085 GTATCC GTATCC(6/5)
BclI 2415 TGATCA T^GATCA
BglII 2440 AGATCT A^GATCT
BlpI 672 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC
BsaBI 644 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 291 GAGACC GGTCTC(1/5)
BsaWI 2932 TCCGGA [AT]^CCGG[AT]
BseAI 2932 TCCGGA T^CCGGA
BseRI 268 GAGGAG GAGGAG(10/8)
BsgI 538 CTGCAC GTGCAG(16/14)
BsiEI 374 CGGCCG CG[GA][CT]^CG
BsmBI 4 GAGACG CGTCTC(1/5)
BspEI 2932 TCCGGA T^CCGGA
BstXI 236 CCAGGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 265 CCTGAGG CC^T[ACGT]AGG
CelII 672 GCTCAGC GC^T[ACGT]AGC
DsaI 2286 CCATGG C^C[GA][CT]GG
EagI 374 CGGCCG C^GGCCG
Eam1105I 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 374 CGGCCG C^GGCCG
Eco47III 208 AGCGCT AGC^GCT
Eco52I 374 CGGCCG C^GGCCG
Eco81I 265 CCTGAGG CC^T[ACGT]AGG
EcoNI 2831 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 2428 GATATC GAT^ATC
EcoT14I 2286 CCATGG C^C[AT][AT]GG
FbaI 2415 TGATCA T^GATCA
HpaI 511 GTTAAC GTT^AAC
MamI 644 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3840 TGGCCA TGG^CCA
MroI 2932 TCCGGA T^CCGGA
MscI 3840 TGGCCA TGG^CCA
NcoI 2286 CCATGG C^CATGG
NotI 373 GCGGCCGC GC^GGCCGC
NruI 198 TCGCGA TCG^CGA
PflMI 2138 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshAI 4706 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 4504 GAGCTC GAGCT^C
SalI 117 GTCGAC G^TCGAC
SphI 3003 GCATGC GCATG^C
StyI 2286 CCATGG C^C[AT][AT]GG
Van91I 2138 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AflIII 1820 ACATGT A^C[GA][CT]GT
3420 ACATGT
AseI 626 ATTAAT AT^TAAT
1733 ATTAAT
AsnI 626 ATTAAT AT^TAAT
1733 ATTAAT
AvaI 1218 CTCGAG C^[CT]CG[GA]G
1653 CTCGAG
BcnI 74 CCGGG CC^[GC]GG
295 CCCGG
BsmI 1156 GAATGC GAATGC(1/-1)
1837 GAATGC
BsoBI 1218 CTCGAG C^[CT]CG[GA]G
1653 CTCGAG
BspLU11I 1820 ACATGT A^CATGT
3420 ACATGT
BstAPI 974 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3530 GCAAGTACTGC
BstEII 2450 GGTAACC G^GT[ACGT]ACC
2892 GGTTACC
BstPI 2450 GGTAACC G^GT[ACGT]ACC
2892 GGTTACC
DraI 749 TTTAAA TTT^AAA
2368 TTTAAA
EaeI 374 CGGCCG [CT]^GGCC[GA]
3840 TGGCCA
EcoO65I 2450 GGTAACC G^GT[ACGT]ACC
2892 GGTTACC
EcoRI 1727 GAATTC G^AATTC
1878 GAATTC
HincII 117 GTCGAC GT[CT]^[GA]AC
511 GTTAAC
HindII 117 GTCGAC GT[CT]^[GA]AC
511 GTTAAC
NciI 74 CCGGG CC^[GC]GG
295 CCCGG
NheI 455 GCTAGC G^CTAGC
4644 GCTAGC
PaeR7I 1218 CTCGAG C^TCGAG
1653 CTCGAG
PshBI 626 ATTAAT AT^TAAT
1733 ATTAAT
PstI 2028 CTGCAG CTGCA^G
2801 CTGCAG
ScaI 3533 AGTACT AGT^ACT
4109 AGTACT
SpeI 4106 ACTAGT A^CTAGT
4388 ACTAGT
StuI 534 AGGCCT AGG^CCT
4087 AGGCCT
XhoI 1218 CTCGAG C^TCGAG
1653 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 106 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AluI AlwI ApoI Asp700I
AspHI AvaII BanI BbeI BbsI
BbvI BfaI BmyI BpuAI BsaHI
BsaJI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI Bst1107I BstF5I BstNI
BstUI BstYI BstZ17I Cac8I CfoI
Cfr13I DdeI DpnII DraII EarI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NarI NdeI NdeII NlaIII
NlaIV NspI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI SspBI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoII
XmnI