################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII Acc65I AclI AflII AgeI ApaI AscI Asp718I AspI AviII AvrII BbrPI BfrI BglI BlnI BpmI BsaAI BsiWI BspDI BspHI BspMI BsrBI BsrFI BssHII BssSI BstBI Cfr10I ClaI CpoI DraIII DrdI EcoT22I FseI FspI HgaI HindIII KpnI KspI MfeI MluI MunI NaeI NgoMIV NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI Psp1406I PvuI RcaI RsrII SacII SapI SbfI SexAI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SwaI Tth111I XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AccIII 2932 TCCGGA T^CCGGA AhdI 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3913 GTGCAC G^TGCAC AlwNI 4048 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 265 CCTGAGG CC^T[ACGT]AGG Aor51HI 208 AGCGCT AGC^GCT ApaLI 3913 GTGCAC G^TGCAC AspEI 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 3840 TGGCCA TGG^CCA BamHI 1660 GGATCC G^GATCC BanII 4504 GAGCTC G[GA]GC[CT]^C BbvCI 863 GCTGAGG CCTCAGC(-5/-2) BcgI 1116 GCAGTTTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3085 GTATCC GTATCC(6/5) BclI 2415 TGATCA T^GATCA BglII 2440 AGATCT A^GATCT BlpI 672 GCTCAGC GC^T[ACGT]AGC Bpu1102I 672 GCTCAGC GC^T[ACGT]AGC BsaBI 644 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 291 GAGACC GGTCTC(1/5) BsaWI 2932 TCCGGA [AT]^CCGG[AT] BseAI 2932 TCCGGA T^CCGGA BseRI 268 GAGGAG GAGGAG(10/8) BsgI 538 CTGCAC GTGCAG(16/14) BsiEI 374 CGGCCG CG[GA][CT]^CG BsmBI 4 GAGACG CGTCTC(1/5) BspEI 2932 TCCGGA T^CCGGA BstXI 236 CCAGGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 265 CCTGAGG CC^T[ACGT]AGG CelII 672 GCTCAGC GC^T[ACGT]AGC DsaI 2286 CCATGG C^C[GA][CT]GG EagI 374 CGGCCG C^GGCCG Eam1105I 1956 GACTCAAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 374 CGGCCG C^GGCCG Eco47III 208 AGCGCT AGC^GCT Eco52I 374 CGGCCG C^GGCCG Eco81I 265 CCTGAGG CC^T[ACGT]AGG EcoNI 2831 CCTGTTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 2428 GATATC GAT^ATC EcoT14I 2286 CCATGG C^C[AT][AT]GG FbaI 2415 TGATCA T^GATCA HpaI 511 GTTAAC GTT^AAC MamI 644 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3840 TGGCCA TGG^CCA MroI 2932 TCCGGA T^CCGGA MscI 3840 TGGCCA TGG^CCA NcoI 2286 CCATGG C^CATGG NotI 373 GCGGCCGC GC^GGCCGC NruI 198 TCGCGA TCG^CGA PflMI 2138 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PshAI 4706 GACTATAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SacI 4504 GAGCTC GAGCT^C SalI 117 GTCGAC G^TCGAC SphI 3003 GCATGC GCATG^C StyI 2286 CCATGG C^C[AT][AT]GG Van91I 2138 CCAGATATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AflIII 1820 ACATGT A^C[GA][CT]GT 3420 ACATGT AseI 626 ATTAAT AT^TAAT 1733 ATTAAT AsnI 626 ATTAAT AT^TAAT 1733 ATTAAT AvaI 1218 CTCGAG C^[CT]CG[GA]G 1653 CTCGAG BcnI 74 CCGGG CC^[GC]GG 295 CCCGG BsmI 1156 GAATGC GAATGC(1/-1) 1837 GAATGC BsoBI 1218 CTCGAG C^[CT]CG[GA]G 1653 CTCGAG BspLU11I 1820 ACATGT A^CATGT 3420 ACATGT BstAPI 974 GCATGTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3530 GCAAGTACTGC BstEII 2450 GGTAACC G^GT[ACGT]ACC 2892 GGTTACC BstPI 2450 GGTAACC G^GT[ACGT]ACC 2892 GGTTACC DraI 749 TTTAAA TTT^AAA 2368 TTTAAA EaeI 374 CGGCCG [CT]^GGCC[GA] 3840 TGGCCA EcoO65I 2450 GGTAACC G^GT[ACGT]ACC 2892 GGTTACC EcoRI 1727 GAATTC G^AATTC 1878 GAATTC HincII 117 GTCGAC GT[CT]^[GA]AC 511 GTTAAC HindII 117 GTCGAC GT[CT]^[GA]AC 511 GTTAAC NciI 74 CCGGG CC^[GC]GG 295 CCCGG NheI 455 GCTAGC G^CTAGC 4644 GCTAGC PaeR7I 1218 CTCGAG C^TCGAG 1653 CTCGAG PshBI 626 ATTAAT AT^TAAT 1733 ATTAAT PstI 2028 CTGCAG CTGCA^G 2801 CTGCAG ScaI 3533 AGTACT AGT^ACT 4109 AGTACT SpeI 4106 ACTAGT A^CTAGT 4388 ACTAGT StuI 534 AGGCCT AGG^CCT 4087 AGGCCT XhoI 1218 CTCGAG C^TCGAG 1653 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 106 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BanI BbeI BbsI BbvI BfaI BmyI BpuAI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI Bst1107I BstF5I BstNI BstUI BstYI BstZ17I Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NdeI NdeII NlaIII NlaIV NspI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI SspBI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI