################################## # NO CUT ENZYMES ( 80 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI AhdI Alw44I Aor51HI ApaLI AscI Asp718I AspEI BbeI BbrPI BbsI BpuAI BsaBI BsaHI BsiEI BsiWI BspDI BspLU11I BsrFI BssHII BstBI BstEII BstPI Cfr10I ClaI CpoI DraIII DrdI DsaI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FseI Hin1I HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NgoMIV NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI Psp1406I PstI PvuI RsrII SacII SalI SapI SbfI ScaI SfoI SfuI SgrAI SnaBI Sse8387I SwaI XhoI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AccII 106 CGCG CG^CG AccIII 1248 TCCGGA T^CCGGA AflII 2315 CTTAAG C^TTAAG AflIII 1777 ACGTGT A^C[GA][CT]GT AlwNI 612 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 3995 GGGCCC GGGCC^C AspI 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 764 TGCGCA TGC^GCA AvrII 1962 CCTAGG C^CTAGG BalI 3342 TGGCCA TGG^CCA BbvCI 2914 CCTCAGC CCTCAGC(-5/-2) BcgI 350 CGACATTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2082 TGATCA T^GATCA BfrI 2315 CTTAAG C^TTAAG BglII 3785 AGATCT A^GATCT BlnI 1962 CCTAGG C^CTAGG BsaI 624 GGTCTC GGTCTC(1/5) BsaWI 1248 TCCGGA [AT]^CCGG[AT] BseAI 1248 TCCGGA T^CCGGA BsmBI 3919 CGTCTC CGTCTC(1/5) BsmFI 608 GTCCC GGGAC(10/14) Bsp1407I 1822 TGTACA T^GTACA BspEI 1248 TCCGGA T^CCGGA BsrBI 296 GAGCGG CCGCTC(-3/-3) BsrGI 1822 TGTACA T^GTACA Bst1107I 2244 GTATAC GTA^TAC BstUI 106 CGCG CG^CG BstZ17I 2244 GTATAC GTA^TAC EcoNI 695 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 3114 GATATC GAT^ATC FbaI 2082 TGATCA T^GATCA FspI 764 TGCGCA TGC^GCA HaeII 2752 GGCGCT [GA]GCGC^[CT] HgaI 1934 GACGC GACGC(5/10) HincII 267 GTCAAC GT[CT]^[GA]AC HindII 267 GTCAAC GT[CT]^[GA]AC MluNI 3342 TGGCCA TGG^CCA MroI 1248 TCCGGA T^CCGGA MscI 3342 TGGCCA TGG^CCA MvnI 106 CGCG CG^CG PflFI 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3267 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 612 CAGCTG CAG^CTG SexAI 271 ACCTGGT A^CC[AT]GGT SpeI 707 ACTAGT A^CTAGT SphI 1737 GCATGC GCATG^C SspBI 1822 TGTACA T^GTACA StuI 2477 AGGCCT AGG^CCT Tth111I 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 215 CCACAGCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 19 enzymes) # ################################### AseI 1604 ATTAAT AT^TAAT 2653 ATTAAT AsnI 1604 ATTAAT AT^TAAT 2653 ATTAAT BamHI 1251 GGATCC G^GATCC 1565 GGATCC BanI 282 GGCACC G^G[CT][GA]CC 2771 GGTGCC BciVI 1223 GGATAC GTATCC(6/5) 488 GTATCC BsaAI 1724 TACGTG [CT]AC^GT[GA] 1776 TACGTG BspMI 1358 GCAGGT ACCTGC(4/8) 1703 GCAGGT BstAPI 1118 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1661 GCACCTCCTGC BstXI 471 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3579 CCACCAACTTGG FauI 93 CCCGC CCCGC(4/6) 452 CCCGC HindIII 805 AAGCTT A^AGCTT 1939 AAGCTT PpuMI 1378 AGGACCT [GA]G^G[AT]CC[CT] 3903 AGGACCC PshBI 1604 ATTAAT AT^TAAT 2653 ATTAAT SacI 41 GAGCTC GAGCT^C 4092 GAGCTC SfiI 124 GGCCCGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 4128 GGCCTGGTCGGCC SspI 2557 AATATT AAT^ATT 2603 AATATT TaqI 349 TCGA T^CGA 1183 TCGA TthHB8I 349 TCGA T^CGA 1183 TCGA XbaI 2364 TCTAGA T^CTAGA 2488 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 109 enzymes) # ######################################### AccI AciI AcsI AfaI AluI AlwI AocI ApoI Asp700I AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmI BsoBI Bsp1286I BspHI BsrDI BsrI BssKI BssSI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII EaeI EarI Eco81I EcoO109I EcoRII EcoT14I EcoT22I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeI NdeII NlaIII NlaIV NsiI NspI PflMI PleI RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII XmaCI XmaI XmnI