##################################
# NO CUT ENZYMES ( 80 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
AhdI Alw44I Aor51HI ApaLI AscI
Asp718I AspEI BbeI BbrPI BbsI
BpuAI BsaBI BsaHI BsiEI BsiWI
BspDI BspLU11I BsrFI BssHII BstBI
BstEII BstPI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRI
FseI Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI Psp1406I
PstI PvuI RsrII SacII SalI
SapI SbfI ScaI SfoI SfuI
SgrAI SnaBI Sse8387I SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AccII 106 CGCG CG^CG
AccIII 1248 TCCGGA T^CCGGA
AflII 2315 CTTAAG C^TTAAG
AflIII 1777 ACGTGT A^C[GA][CT]GT
AlwNI 612 CAGCTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3995 GGGCCC GGGCC^C
AspI 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 764 TGCGCA TGC^GCA
AvrII 1962 CCTAGG C^CTAGG
BalI 3342 TGGCCA TGG^CCA
BbvCI 2914 CCTCAGC CCTCAGC(-5/-2)
BcgI 350 CGACATTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2082 TGATCA T^GATCA
BfrI 2315 CTTAAG C^TTAAG
BglII 3785 AGATCT A^GATCT
BlnI 1962 CCTAGG C^CTAGG
BsaI 624 GGTCTC GGTCTC(1/5)
BsaWI 1248 TCCGGA [AT]^CCGG[AT]
BseAI 1248 TCCGGA T^CCGGA
BsmBI 3919 CGTCTC CGTCTC(1/5)
BsmFI 608 GTCCC GGGAC(10/14)
Bsp1407I 1822 TGTACA T^GTACA
BspEI 1248 TCCGGA T^CCGGA
BsrBI 296 GAGCGG CCGCTC(-3/-3)
BsrGI 1822 TGTACA T^GTACA
Bst1107I 2244 GTATAC GTA^TAC
BstUI 106 CGCG CG^CG
BstZ17I 2244 GTATAC GTA^TAC
EcoNI 695 CCTGTCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 3114 GATATC GAT^ATC
FbaI 2082 TGATCA T^GATCA
FspI 764 TGCGCA TGC^GCA
HaeII 2752 GGCGCT [GA]GCGC^[CT]
HgaI 1934 GACGC GACGC(5/10)
HincII 267 GTCAAC GT[CT]^[GA]AC
HindII 267 GTCAAC GT[CT]^[GA]AC
MluNI 3342 TGGCCA TGG^CCA
MroI 1248 TCCGGA T^CCGGA
MscI 3342 TGGCCA TGG^CCA
MvnI 106 CGCG CG^CG
PflFI 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3267 GACAAAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 612 CAGCTG CAG^CTG
SexAI 271 ACCTGGT A^CC[AT]GGT
SpeI 707 ACTAGT A^CTAGT
SphI 1737 GCATGC GCATG^C
SspBI 1822 TGTACA T^GTACA
StuI 2477 AGGCCT AGG^CCT
Tth111I 3598 GACCCGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 215 CCACAGCAGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AseI 1604 ATTAAT AT^TAAT
2653 ATTAAT
AsnI 1604 ATTAAT AT^TAAT
2653 ATTAAT
BamHI 1251 GGATCC G^GATCC
1565 GGATCC
BanI 282 GGCACC G^G[CT][GA]CC
2771 GGTGCC
BciVI 1223 GGATAC GTATCC(6/5)
488 GTATCC
BsaAI 1724 TACGTG [CT]AC^GT[GA]
1776 TACGTG
BspMI 1358 GCAGGT ACCTGC(4/8)
1703 GCAGGT
BstAPI 1118 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1661 GCACCTCCTGC
BstXI 471 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3579 CCACCAACTTGG
FauI 93 CCCGC CCCGC(4/6)
452 CCCGC
HindIII 805 AAGCTT A^AGCTT
1939 AAGCTT
PpuMI 1378 AGGACCT [GA]G^G[AT]CC[CT]
3903 AGGACCC
PshBI 1604 ATTAAT AT^TAAT
2653 ATTAAT
SacI 41 GAGCTC GAGCT^C
4092 GAGCTC
SfiI 124 GGCCCGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
4128 GGCCTGGTCGGCC
SspI 2557 AATATT AAT^ATT
2603 AATATT
TaqI 349 TCGA T^CGA
1183 TCGA
TthHB8I 349 TCGA T^CGA
1183 TCGA
XbaI 2364 TCTAGA T^CTAGA
2488 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI AocI ApoI Asp700I AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmI BsoBI
Bsp1286I BspHI BsrDI BsrI BssKI
BssSI BstF5I BstNI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraI DraII EaeI EarI
Eco81I EcoO109I EcoRII EcoT14I EcoT22I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeI NdeII NlaIII NlaIV NsiI
NspI PflMI PleI RcaI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI SmlI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII XmaCI XmaI XmnI