##################################
# NO CUT ENZYMES (   80 enzymes) #
##################################

AatII       Acc65I      AclI        AcyI        AgeI        
AhdI        Alw44I      Aor51HI     ApaLI       AscI        
Asp718I     AspEI       BbeI        BbrPI       BbsI        
BpuAI       BsaBI       BsaHI       BsiEI       BsiWI       
BspDI       BspLU11I    BsrFI       BssHII      BstBI       
BstEII      BstPI       Cfr10I      ClaI        CpoI        
DraIII      DrdI        DsaI        EagI        Eam1105I    
EclXI       Eco47III    Eco52I      EcoO65I     EcoRI       
FseI        Hin1I       HpaI        KasI        KpnI        
KspI        MamI        MfeI        MluI        MunI        
NaeI        NarI        NcoI        NgoMIV      NheI        
NotI        NruI        NspV        PacI        PaeR7I      
PinAI       PmaCI       PmeI        PmlI        Psp1406I    
PstI        PvuI        RsrII       SacII       SalI        
SapI        SbfI        ScaI        SfoI        SfuI        
SgrAI       SnaBI       Sse8387I    SwaI        XhoI        


###################################
# ONE CUT ENZYMES (   50 enzymes) #
###################################

AccII         106  CGCG                 CG^CG
AccIII       1248  TCCGGA               T^CCGGA
AflII        2315  CTTAAG               C^TTAAG
AflIII       1777  ACGTGT               A^C[GA][CT]GT
AlwNI         612  CAGCTGCTG            CAG[ACGT][ACGT][ACGT]^CTG
ApaI         3995  GGGCCC               GGGCC^C
AspI         3598  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AviII         764  TGCGCA               TGC^GCA
AvrII        1962  CCTAGG               C^CTAGG
BalI         3342  TGGCCA               TGG^CCA
BbvCI        2914  CCTCAGC              CCTCAGC(-5/-2)
BcgI          350  CGACATTACTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2082  TGATCA               T^GATCA
BfrI         2315  CTTAAG               C^TTAAG
BglII        3785  AGATCT               A^GATCT
BlnI         1962  CCTAGG               C^CTAGG
BsaI          624  GGTCTC               GGTCTC(1/5)
BsaWI        1248  TCCGGA               [AT]^CCGG[AT]
BseAI        1248  TCCGGA               T^CCGGA
BsmBI        3919  CGTCTC               CGTCTC(1/5)
BsmFI         608  GTCCC                GGGAC(10/14)
Bsp1407I     1822  TGTACA               T^GTACA
BspEI        1248  TCCGGA               T^CCGGA
BsrBI         296  GAGCGG               CCGCTC(-3/-3)
BsrGI        1822  TGTACA               T^GTACA
Bst1107I     2244  GTATAC               GTA^TAC
BstUI         106  CGCG                 CG^CG
BstZ17I      2244  GTATAC               GTA^TAC
EcoNI         695  CCTGTCACAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV        3114  GATATC               GAT^ATC
FbaI         2082  TGATCA               T^GATCA
FspI          764  TGCGCA               TGC^GCA
HaeII        2752  GGCGCT               [GA]GCGC^[CT]
HgaI         1934  GACGC                GACGC(5/10)
HincII        267  GTCAAC               GT[CT]^[GA]AC
HindII        267  GTCAAC               GT[CT]^[GA]AC
MluNI        3342  TGGCCA               TGG^CCA
MroI         1248  TCCGGA               T^CCGGA
MscI         3342  TGGCCA               TGG^CCA
MvnI          106  CGCG                 CG^CG
PflFI        3598  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PshAI        3267  GACAAAAGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         612  CAGCTG               CAG^CTG
SexAI         271  ACCTGGT              A^CC[AT]GGT
SpeI          707  ACTAGT               A^CTAGT
SphI         1737  GCATGC               GCATG^C
SspBI        1822  TGTACA               T^GTACA
StuI         2477  AGGCCT               AGG^CCT
Tth111I      3598  GACCCGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XcmI          215  CCACAGCAGGACTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (   19 enzymes) #
###################################

AseI         1604  ATTAAT               AT^TAAT
             2653  ATTAAT              
AsnI         1604  ATTAAT               AT^TAAT
             2653  ATTAAT              
BamHI        1251  GGATCC               G^GATCC
             1565  GGATCC              
BanI          282  GGCACC               G^G[CT][GA]CC
             2771  GGTGCC              
BciVI        1223  GGATAC               GTATCC(6/5)
              488  GTATCC              
BsaAI        1724  TACGTG               [CT]AC^GT[GA]
             1776  TACGTG              
BspMI        1358  GCAGGT               ACCTGC(4/8)
             1703  GCAGGT              
BstAPI       1118  GCAGATCATGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             1661  GCACCTCCTGC         
BstXI         471  CCAAAAGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             3579  CCACCAACTTGG        
FauI           93  CCCGC                CCCGC(4/6)
              452  CCCGC               
HindIII       805  AAGCTT               A^AGCTT
             1939  AAGCTT              
PpuMI        1378  AGGACCT              [GA]G^G[AT]CC[CT]
             3903  AGGACCC             
PshBI        1604  ATTAAT               AT^TAAT
             2653  ATTAAT              
SacI           41  GAGCTC               GAGCT^C
             4092  GAGCTC              
SfiI          124  GGCCCGGCTGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
             4128  GGCCTGGTCGGCC       
SspI         2557  AATATT               AAT^ATT
             2603  AATATT              
TaqI          349  TCGA                 T^CGA
             1183  TCGA                
TthHB8I       349  TCGA                 T^CGA
             1183  TCGA                
XbaI         2364  TCTAGA               T^CTAGA
             2488  TCTAGA              


#########################################
# 3 OR MORE CUT ENZYMES (  109 enzymes) #
#########################################

AccI        AciI        AcsI        AfaI        AluI        
AlwI        AocI        ApoI        Asp700I     AspHI       
AvaI        AvaII       BanII       BbvI        BcnI        
BfaI        BglI        BlpI        BmyI        BpmI        
Bpu1102I    BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmI        BsoBI       
Bsp1286I    BspHI       BsrDI       BsrI        BssKI       
BssSI       BstF5I      BstNI       BstYI       Bsu36I      
Cac8I       CelII       CfoI        Cfr13I      DdeI        
DpnII       DraI        DraII       EaeI        EarI        
Eco81I      EcoO109I    EcoRII      EcoT14I     EcoT22I     
Fnu4HI      FokI        HaeIII      HapII       HhaI        
HinP1I      HinfI       HpaII       HphI        ItaI        
Ksp632I     MaeI        MaeII       MaeIII      MboI        
MboII       MflI        MnlI        MseI        MslI        
MspA1I      MspI        MvaI        MwoI        NciI        
NdeI        NdeII       NlaIII      NlaIV       NsiI        
NspI        PflMI       PleI        RcaI        RsaI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmaI        SmlI        StyI        TfiI        Tru9I       
TseI        Tsp45I      Tsp509I     TspRI       Van91I      
XhoII       XmaCI       XmaI        XmnI