################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## Acc65I AccIII AclI AgeI AscI AseI AsnI Asp700I Asp718I AviII AvrII BamHI BbrPI BlnI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BsrDI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI EcoRV EcoT22I FspI HgaI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SalI SbfI SfiI SfuI SgrAI SmaI SnaBI Sse8387I SspBI SwaI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AccII 16 CGCG CG^CG AflII 4312 CTTAAG C^TTAAG AhdI 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 3906 AGCGCT AGC^GCT ApaI 3189 GGGCCC GGGCC^C AspEI 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC BanI 1242 GGCGCC G^G[CT][GA]CC BbeI 1242 GGCGCC GGCGC^C BbvCI 3885 GCTGAGG CCTCAGC(-5/-2) BcgI 3912 GCAGAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 4700 GGATAC GTATCC(6/5) BclI 530 TGATCA T^GATCA BfrI 4312 CTTAAG C^TTAAG BglII 1796 AGATCT A^GATCT BsaAI 2739 TACGTG [CT]AC^GT[GA] BsaWI 130 TCCGGT [AT]^CCGG[AT] BspHI 1347 TCATGA T^CATGA BsrBI 941 GAGCGG CCGCTC(-3/-3) BssSI 3240 CTCGTG CACGAG(-5/-1) BstEII 2438 GGTGACC G^GT[ACGT]ACC BstPI 2438 GGTGACC G^GT[ACGT]ACC BstUI 16 CGCG CG^CG DrdI 1971 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 3906 AGCGCT AGC^GCT EcoO65I 2438 GGTGACC G^GT[ACGT]ACC FbaI 530 TGATCA T^GATCA FseI 680 GGCCGGCC GGCCGG^CC KasI 1242 GGCGCC G^GCGCC KspI 15 CCGCGG CCGC^GG MvnI 16 CGCG CG^CG NarI 1242 GGCGCC GG^CGCC NcoI 3247 CCATGG C^CATGG NdeI 3486 CATATG CA^TATG PflFI 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshAI 3396 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 1347 TCATGA T^CATGA SacII 15 CCGCGG CCGC^GG SexAI 2061 ACCTGGT A^CC[AT]GGT SfoI 1242 GGCGCC GGC^GCC SphI 2179 GCATGC GCATG^C SspI 2077 AATATT AAT^ATT Tth111I 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 4093 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 1144 GTGCAC G^TGCAC 2668 GTGCAC ApaLI 1144 GTGCAC G^TGCAC 2668 GTGCAC BsgI 4145 CTGCAC GTGCAG(16/14) 1046 GTGCAG BsiEI 679 CGGCCG CG[GA][CT]^CG 683 CGGCCG BsmBI 38 CGTCTC CGTCTC(1/5) 2240 CGTCTC BsrFI 677 GCCGGC [GA]^CCGG[CT] 681 GCCGGC BstAPI 290 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 4358 GCACTGACTGC Cfr10I 677 GCCGGC [GA]^CCGG[CT] 681 GCCGGC DraIII 4403 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG 4816 CACATGGTG EagI 679 CGGCCG C^GGCCG 683 CGGCCG EclXI 679 CGGCCG C^GGCCG 683 CGGCCG Eco52I 679 CGGCCG C^GGCCG 683 CGGCCG HaeII 1242 GGCGCC [GA]GCGC^[CT] 3906 AGCGCT HincII 405 GTCAAC GT[CT]^[GA]AC 3149 GTTGAC HindII 405 GTCAAC GT[CT]^[GA]AC 3149 GTTGAC NaeI 677 GCCGGC GCC^GGC 681 GCCGGC NgoMIV 677 GCCGGC G^CCGGC 681 GCCGGC SacI 2646 GAGCTC GAGCT^C 3203 GAGCTC ScaI 500 AGTACT AGT^ACT 1651 AGTACT SpeI 4076 ACTAGT A^CTAGT 4239 ACTAGT XcmI 4395 CCAGCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 4808 CCAGCAACCACATGG ######################################### # 3 OR MORE CUT ENZYMES ( 125 enzymes) # ######################################### AatII AccI AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI AocI ApoI AspHI AvaI AvaII BalI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstYI Bsu36I Cac8I CelII CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoRI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NheI NlaIII NlaIV NspI PaeR7I PflMI PleI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI XhoII