##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
Acc65I AccIII AclI AgeI AscI
AseI AsnI Asp700I Asp718I AviII
AvrII BamHI BbrPI BlnI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrDI BsrGI BssHII Bst1107I BstBI
BstXI BstZ17I ClaI CpoI EcoRV
EcoT22I FspI HgaI HindIII HpaI
KpnI MamI MfeI MluI MroI
MunI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PvuI RsrII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SspBI SwaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccII 16 CGCG CG^CG
AflII 4312 CTTAAG C^TTAAG
AhdI 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 3906 AGCGCT AGC^GCT
ApaI 3189 GGGCCC GGGCC^C
AspEI 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1242 GGCGCC G^G[CT][GA]CC
BbeI 1242 GGCGCC GGCGC^C
BbvCI 3885 GCTGAGG CCTCAGC(-5/-2)
BcgI 3912 GCAGAATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 4700 GGATAC GTATCC(6/5)
BclI 530 TGATCA T^GATCA
BfrI 4312 CTTAAG C^TTAAG
BglII 1796 AGATCT A^GATCT
BsaAI 2739 TACGTG [CT]AC^GT[GA]
BsaWI 130 TCCGGT [AT]^CCGG[AT]
BspHI 1347 TCATGA T^CATGA
BsrBI 941 GAGCGG CCGCTC(-3/-3)
BssSI 3240 CTCGTG CACGAG(-5/-1)
BstEII 2438 GGTGACC G^GT[ACGT]ACC
BstPI 2438 GGTGACC G^GT[ACGT]ACC
BstUI 16 CGCG CG^CG
DrdI 1971 GACCCACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 149 GACTGTCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 3906 AGCGCT AGC^GCT
EcoO65I 2438 GGTGACC G^GT[ACGT]ACC
FbaI 530 TGATCA T^GATCA
FseI 680 GGCCGGCC GGCCGG^CC
KasI 1242 GGCGCC G^GCGCC
KspI 15 CCGCGG CCGC^GG
MvnI 16 CGCG CG^CG
NarI 1242 GGCGCC GG^CGCC
NcoI 3247 CCATGG C^CATGG
NdeI 3486 CATATG CA^TATG
PflFI 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 3396 GACTGTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1347 TCATGA T^CATGA
SacII 15 CCGCGG CCGC^GG
SexAI 2061 ACCTGGT A^CC[AT]GGT
SfoI 1242 GGCGCC GGC^GCC
SphI 2179 GCATGC GCATG^C
SspI 2077 AATATT AAT^ATT
Tth111I 727 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 4093 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1144 GTGCAC G^TGCAC
2668 GTGCAC
ApaLI 1144 GTGCAC G^TGCAC
2668 GTGCAC
BsgI 4145 CTGCAC GTGCAG(16/14)
1046 GTGCAG
BsiEI 679 CGGCCG CG[GA][CT]^CG
683 CGGCCG
BsmBI 38 CGTCTC CGTCTC(1/5)
2240 CGTCTC
BsrFI 677 GCCGGC [GA]^CCGG[CT]
681 GCCGGC
BstAPI 290 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
4358 GCACTGACTGC
Cfr10I 677 GCCGGC [GA]^CCGG[CT]
681 GCCGGC
DraIII 4403 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
4816 CACATGGTG
EagI 679 CGGCCG C^GGCCG
683 CGGCCG
EclXI 679 CGGCCG C^GGCCG
683 CGGCCG
Eco52I 679 CGGCCG C^GGCCG
683 CGGCCG
HaeII 1242 GGCGCC [GA]GCGC^[CT]
3906 AGCGCT
HincII 405 GTCAAC GT[CT]^[GA]AC
3149 GTTGAC
HindII 405 GTCAAC GT[CT]^[GA]AC
3149 GTTGAC
NaeI 677 GCCGGC GCC^GGC
681 GCCGGC
NgoMIV 677 GCCGGC G^CCGGC
681 GCCGGC
SacI 2646 GAGCTC GAGCT^C
3203 GAGCTC
ScaI 500 AGTACT AGT^ACT
1651 AGTACT
SpeI 4076 ACTAGT A^CTAGT
4239 ACTAGT
XcmI 4395 CCAGCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4808 CCAGCAACCACATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 125 enzymes) #
#########################################
AatII AccI AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
AocI ApoI AspHI AvaI AvaII
BalI BanII BbsI BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BpuAI BsaHI BsaI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Bsu36I Cac8I CelII CfoI
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI Eco81I EcoNI
EcoO109I EcoRI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NheI
NlaIII NlaIV NspI PaeR7I PflMI
PleI PpuMI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XhoI XhoII