##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AviII BfrI BglII BsaWI BseAI
BsiEI BsiWI BsmI BspEI BspHI
BssSI Bst1107I BstAPI BstBI BstZ17I
Bsu36I DrdI EagI EclXI Eco52I
Eco81I EcoRI EcoRV EcoT22I FseI
FspI HindIII HpaI KpnI MluI
MroI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RcaI SfuI SgrAI
SpeI SwaI Tth111I XbaI XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AcsI 1996 AAATTT [GA]^AATT[CT]
AcyI 2132 GGCGCC G[GA]^CG[CT]C
AhdI 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 909 AGCGCT AGC^GCT
ApoI 1996 AAATTT [GA]^AATT[CT]
AspEI 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1148 GGATCC G^GATCC
BbeI 2132 GGCGCC GGCGC^C
BbsI 2984 GAAGAC GAAGAC(2/6)
BcgI 2962 GCACCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1370 TGATCA T^GATCA
BpuAI 2984 GAAGAC GAAGAC(2/6)
BsaBI 2690 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2132 GGCGCC G[GA]^CG[CT]C
BsmBI 1518 GAGACG CGTCTC(1/5)
BspDI 1677 ATCGAT AT^CGAT
BsrFI 3402 ACCGGC [GA]^CCGG[CT]
BssHII 98 GCGCGC G^CGCGC
BstEII 2522 GGTGACC G^GT[ACGT]ACC
BstPI 2522 GGTGACC G^GT[ACGT]ACC
Cfr10I 3402 ACCGGC [GA]^CCGG[CT]
ClaI 1677 ATCGAT AT^CGAT
CpoI 3217 CGGACCG CG^G[AT]CCG
DraI 3882 TTTAAA TTT^AAA
Eam1105I 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 909 AGCGCT AGC^GCT
EcoO65I 2522 GGTGACC G^GT[ACGT]ACC
FbaI 1370 TGATCA T^GATCA
HgaI 494 GCGTC GACGC(5/10)
Hin1I 2132 GGCGCC G[GA]^CG[CT]C
KasI 2132 GGCGCC G^GCGCC
MamI 2690 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 1291 CAATTG C^AATTG
MunI 1291 CAATTG C^AATTG
NarI 2132 GGCGCC GG^CGCC
RsrII 3217 CGGACCG CG^G[AT]CCG
SalI 3238 GTCGAC G^TCGAC
SbfI 2060 CCTGCAGG CCTGCA^GG
SfoI 2132 GGCGCC GGC^GCC
SnaBI 1226 TACGTA TAC^GTA
Sse8387I 2060 CCTGCAGG CCTGCA^GG
SspI 1414 AATATT AAT^ATT
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 3238 GTCGAC GT^[AC][GT]AC
3603 GTCTAC
AlwNI 2229 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2304 CAGCAACTG
AvrII 3023 CCTAGG C^CTAGG
3392 CCTAGG
BbrPI 965 CACGTG CAC^GTG
1860 CACGTG
BlnI 3023 CCTAGG C^CTAGG
3392 CCTAGG
BsaI 2286 GAGACC GGTCTC(1/5)
3833 GAGACC
Bsp1407I 298 TGTACA T^GTACA
2261 TGTACA
BsrBI 40 GAGCGG CCGCTC(-3/-3)
3823 GAGCGG
BsrDI 1319 CATTGC GCAATG(2/0)
3756 GCAATG
BsrGI 298 TGTACA T^GTACA
2261 TGTACA
BstXI 527 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2908 CCAACACCTTGG
DraIII 3417 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
3447 CACACCGTG
EcoNI 1061 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2464 CCTCCTCCAGG
HincII 3238 GTCGAC GT[CT]^[GA]AC
3636 GTCAAC
HindII 3238 GTCGAC GT[CT]^[GA]AC
3636 GTCAAC
PflMI 1784 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3416 CCACCTGGTGG
PmaCI 965 CACGTG CAC^GTG
1860 CACGTG
PmlI 965 CACGTG CAC^GTG
1860 CACGTG
PpuMI 3490 AGGTCCC [GA]G^G[AT]CC[CT]
3514 GGGTCCT
PshAI 1913 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2687 GACGATTGTC
SapI 591 GAAGAGC GCTCTTC(1/4)
3118 GCTCTTC
ScaI 414 AGTACT AGT^ACT
719 AGTACT
SexAI 3418 ACCTGGT A^CC[AT]GGT
3487 ACCAGGT
SfiI 143 GGCCCCGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1949 GGCCGCGGTGGCC
SmaI 2668 CCCGGG CCC^GGG
3511 CCCGGG
SspBI 298 TGTACA T^GTACA
2261 TGTACA
Van91I 1784 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3416 CCACCTGGTGG
XmaCI 2668 CCCGGG C^CCGGG
3511 CCCGGG
XmaI 2668 CCCGGG C^CCGGG
3511 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbvCI BbvI
BciVI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PleI PstI PvuII RsaI
SacI SacII Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SphI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII