################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AclI AflII AgeI Alw44I AocI ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII BfrI BglII BsaWI BseAI BsiEI BsiWI BsmI BspEI BspHI BssSI Bst1107I BstAPI BstBI BstZ17I Bsu36I DrdI EagI EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KpnI MluI MroI NaeI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI SfuI SgrAI SpeI SwaI Tth111I XbaI XhoI XmnI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AcsI 1996 AAATTT [GA]^AATT[CT] AcyI 2132 GGCGCC G[GA]^CG[CT]C AhdI 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 909 AGCGCT AGC^GCT ApoI 1996 AAATTT [GA]^AATT[CT] AspEI 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 1148 GGATCC G^GATCC BbeI 2132 GGCGCC GGCGC^C BbsI 2984 GAAGAC GAAGAC(2/6) BcgI 2962 GCACCATCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1370 TGATCA T^GATCA BpuAI 2984 GAAGAC GAAGAC(2/6) BsaBI 2690 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2132 GGCGCC G[GA]^CG[CT]C BsmBI 1518 GAGACG CGTCTC(1/5) BspDI 1677 ATCGAT AT^CGAT BsrFI 3402 ACCGGC [GA]^CCGG[CT] BssHII 98 GCGCGC G^CGCGC BstEII 2522 GGTGACC G^GT[ACGT]ACC BstPI 2522 GGTGACC G^GT[ACGT]ACC Cfr10I 3402 ACCGGC [GA]^CCGG[CT] ClaI 1677 ATCGAT AT^CGAT CpoI 3217 CGGACCG CG^G[AT]CCG DraI 3882 TTTAAA TTT^AAA Eam1105I 1296 GACATGTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 909 AGCGCT AGC^GCT EcoO65I 2522 GGTGACC G^GT[ACGT]ACC FbaI 1370 TGATCA T^GATCA HgaI 494 GCGTC GACGC(5/10) Hin1I 2132 GGCGCC G[GA]^CG[CT]C KasI 2132 GGCGCC G^GCGCC MamI 2690 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 1291 CAATTG C^AATTG MunI 1291 CAATTG C^AATTG NarI 2132 GGCGCC GG^CGCC RsrII 3217 CGGACCG CG^G[AT]CCG SalI 3238 GTCGAC G^TCGAC SbfI 2060 CCTGCAGG CCTGCA^GG SfoI 2132 GGCGCC GGC^GCC SnaBI 1226 TACGTA TAC^GTA Sse8387I 2060 CCTGCAGG CCTGCA^GG SspI 1414 AATATT AAT^ATT ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AccI 3238 GTCGAC GT^[AC][GT]AC 3603 GTCTAC AlwNI 2229 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG 2304 CAGCAACTG AvrII 3023 CCTAGG C^CTAGG 3392 CCTAGG BbrPI 965 CACGTG CAC^GTG 1860 CACGTG BlnI 3023 CCTAGG C^CTAGG 3392 CCTAGG BsaI 2286 GAGACC GGTCTC(1/5) 3833 GAGACC Bsp1407I 298 TGTACA T^GTACA 2261 TGTACA BsrBI 40 GAGCGG CCGCTC(-3/-3) 3823 GAGCGG BsrDI 1319 CATTGC GCAATG(2/0) 3756 GCAATG BsrGI 298 TGTACA T^GTACA 2261 TGTACA BstXI 527 CCAAAAATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2908 CCAACACCTTGG DraIII 3417 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG 3447 CACACCGTG EcoNI 1061 CCTCCAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2464 CCTCCTCCAGG HincII 3238 GTCGAC GT[CT]^[GA]AC 3636 GTCAAC HindII 3238 GTCGAC GT[CT]^[GA]AC 3636 GTCAAC PflMI 1784 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3416 CCACCTGGTGG PmaCI 965 CACGTG CAC^GTG 1860 CACGTG PmlI 965 CACGTG CAC^GTG 1860 CACGTG PpuMI 3490 AGGTCCC [GA]G^G[AT]CC[CT] 3514 GGGTCCT PshAI 1913 GACCACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2687 GACGATTGTC SapI 591 GAAGAGC GCTCTTC(1/4) 3118 GCTCTTC ScaI 414 AGTACT AGT^ACT 719 AGTACT SexAI 3418 ACCTGGT A^CC[AT]GGT 3487 ACCAGGT SfiI 143 GGCCCCGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 1949 GGCCGCGGTGGCC SmaI 2668 CCCGGG CCC^GGG 3511 CCCGGG SspBI 298 TGTACA T^GTACA 2261 TGTACA Van91I 1784 CCAAAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3416 CCACCTGGTGG XmaCI 2668 CCCGGG C^CCGGG 3511 CCCGGG XmaI 2668 CCCGGG C^CCGGG 3511 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI ApaI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI SacI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII