################################## # NO CUT ENZYMES ( 69 enzymes) # ################################## Acc65I AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI Asp718I AspEI AspI AvrII BbrPI BfrI BglII BlnI BsaAI BsiWI BspDI BspHI BsrBI BssHII BssSI Bst1107I BstBI BstZ17I ClaI DraI DraIII Eam1105I Eco47III EcoRI EcoRV FseI HindIII HpaI KpnI MfeI MluI MunI NaeI NgoMIV NheI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI SacI SapI SbfI SfuI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### AatII 1924 GACGTC GACGT^C AccIII 4716 TCCGGA T^CCGGA AflIII 2967 ACATGT A^C[GA][CT]GT AviII 2755 TGCGCA TGC^GCA BamHI 3078 GGATCC G^GATCC BbeI 3 GGCGCC GGCGC^C BbsI 2238 GAAGAC GAAGAC(2/6) BciVI 4446 GGATAC GTATCC(6/5) BclI 1128 TGATCA T^GATCA BpuAI 2238 GAAGAC GAAGAC(2/6) BsaBI 1026 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 4716 TCCGGA [AT]^CCGG[AT] BseAI 4716 TCCGGA T^CCGGA BspEI 4716 TCCGGA T^CCGGA BspLU11I 2967 ACATGT A^CATGT BstAPI 3472 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC CpoI 4718 CGGACCG CG^G[AT]CCG DrdI 327 GACCGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoT22I 1397 ATGCAT ATGCA^T FbaI 1128 TGATCA T^GATCA FspI 2755 TGCGCA TGC^GCA HincII 2565 GTCGAC GT[CT]^[GA]AC HindII 2565 GTCGAC GT[CT]^[GA]AC KasI 3 GGCGCC G^GCGCC KspI 136 CCGCGG CCGC^GG MamI 1026 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 4716 TCCGGA T^CCGGA NarI 3 GGCGCC GG^CGCC NdeI 4738 CATATG CA^TATG NotI 156 GCGGCCGC GC^GGCCGC NsiI 1397 ATGCAT ATGCA^T PshAI 2964 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RsrII 4718 CGGACCG CG^G[AT]CCG SacII 136 CCGCGG CCGC^GG SalI 2565 GTCGAC G^TCGAC SfiI 1746 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 3 GGCGCC GGC^GCC SgrAI 2287 CGCCGGTG C[GA]^CCGG[CT]G StuI 2416 AGGCCT AGG^CCT ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 1671 GTCTAC GT^[AC][GT]AC 2565 GTCGAC AcsI 396 GAATTT [GA]^AATT[CT] 1580 GAATTT ApoI 396 GAATTT [GA]^AATT[CT] 1580 GAATTT Asp700I 393 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 396 GAATTTCTTC BbvCI 3362 CCTCAGC CCTCAGC(-5/-2) 1793 GCTGAGG BcgI 2610 CGAGCCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 4040 GCATCTGCCTCG BlpI 1157 GCTTAGC GC^T[ACGT]AGC 2336 GCTCAGC Bpu1102I 1157 GCTTAGC GC^T[ACGT]AGC 2336 GCTCAGC BsiEI 157 CGGCCG CG[GA][CT]^CG 3557 CGGCCG BsmBI 282 GAGACG CGTCTC(1/5) 3811 GAGACG BsmI 2105 GAATGC GAATGC(1/-1) 2559 GAATGC Bsp1407I 3925 TGTACA T^GTACA 4734 TGTACA BsrFI 86 ACCGGC [GA]^CCGG[CT] 2288 GCCGGT BsrGI 3925 TGTACA T^GTACA 4734 TGTACA CelII 1157 GCTTAGC GC^T[ACGT]AGC 2336 GCTCAGC Cfr10I 86 ACCGGC [GA]^CCGG[CT] 2288 GCCGGT EagI 157 CGGCCG C^GGCCG 3557 CGGCCG EclXI 157 CGGCCG C^GGCCG 3557 CGGCCG Eco52I 157 CGGCCG C^GGCCG 3557 CGGCCG EcoNI 2534 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 4306 CCTCAGGGAGG PvuII 2389 CAGCTG CAG^CTG 2513 CAGCTG ScaI 687 AGTACT AGT^ACT 2370 AGTACT SexAI 884 ACCAGGT A^CC[AT]GGT 3067 ACCAGGT SphI 1470 GCATGC GCATG^C 4620 GCATGC SspBI 3925 TGTACA T^GTACA 4734 TGTACA XmnI 393 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 396 GAATTTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 124 enzymes) # ######################################### AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI AocI ApaI ApaLI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI