##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
Acc65I AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp718I
AspEI AspI AvrII BbrPI BfrI
BglII BlnI BsaAI BsiWI BspDI
BspHI BsrBI BssHII BssSI Bst1107I
BstBI BstZ17I ClaI DraI DraIII
Eam1105I Eco47III EcoRI EcoRV FseI
HindIII HpaI KpnI MfeI MluI
MunI NaeI NgoMIV NheI NruI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RcaI SacI SapI SbfI
SfuI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AatII 1924 GACGTC GACGT^C
AccIII 4716 TCCGGA T^CCGGA
AflIII 2967 ACATGT A^C[GA][CT]GT
AviII 2755 TGCGCA TGC^GCA
BamHI 3078 GGATCC G^GATCC
BbeI 3 GGCGCC GGCGC^C
BbsI 2238 GAAGAC GAAGAC(2/6)
BciVI 4446 GGATAC GTATCC(6/5)
BclI 1128 TGATCA T^GATCA
BpuAI 2238 GAAGAC GAAGAC(2/6)
BsaBI 1026 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 4716 TCCGGA [AT]^CCGG[AT]
BseAI 4716 TCCGGA T^CCGGA
BspEI 4716 TCCGGA T^CCGGA
BspLU11I 2967 ACATGT A^CATGT
BstAPI 3472 GCATCAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
CpoI 4718 CGGACCG CG^G[AT]CCG
DrdI 327 GACCGAGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1397 ATGCAT ATGCA^T
FbaI 1128 TGATCA T^GATCA
FspI 2755 TGCGCA TGC^GCA
HincII 2565 GTCGAC GT[CT]^[GA]AC
HindII 2565 GTCGAC GT[CT]^[GA]AC
KasI 3 GGCGCC G^GCGCC
KspI 136 CCGCGG CCGC^GG
MamI 1026 GATTGGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 4716 TCCGGA T^CCGGA
NarI 3 GGCGCC GG^CGCC
NdeI 4738 CATATG CA^TATG
NotI 156 GCGGCCGC GC^GGCCGC
NsiI 1397 ATGCAT ATGCA^T
PshAI 2964 GACACATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RsrII 4718 CGGACCG CG^G[AT]CCG
SacII 136 CCGCGG CCGC^GG
SalI 2565 GTCGAC G^TCGAC
SfiI 1746 GGCCTGGGGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3 GGCGCC GGC^GCC
SgrAI 2287 CGCCGGTG C[GA]^CCGG[CT]G
StuI 2416 AGGCCT AGG^CCT
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1671 GTCTAC GT^[AC][GT]AC
2565 GTCGAC
AcsI 396 GAATTT [GA]^AATT[CT]
1580 GAATTT
ApoI 396 GAATTT [GA]^AATT[CT]
1580 GAATTT
Asp700I 393 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
396 GAATTTCTTC
BbvCI 3362 CCTCAGC CCTCAGC(-5/-2)
1793 GCTGAGG
BcgI 2610 CGAGCCGTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
4040 GCATCTGCCTCG
BlpI 1157 GCTTAGC GC^T[ACGT]AGC
2336 GCTCAGC
Bpu1102I 1157 GCTTAGC GC^T[ACGT]AGC
2336 GCTCAGC
BsiEI 157 CGGCCG CG[GA][CT]^CG
3557 CGGCCG
BsmBI 282 GAGACG CGTCTC(1/5)
3811 GAGACG
BsmI 2105 GAATGC GAATGC(1/-1)
2559 GAATGC
Bsp1407I 3925 TGTACA T^GTACA
4734 TGTACA
BsrFI 86 ACCGGC [GA]^CCGG[CT]
2288 GCCGGT
BsrGI 3925 TGTACA T^GTACA
4734 TGTACA
CelII 1157 GCTTAGC GC^T[ACGT]AGC
2336 GCTCAGC
Cfr10I 86 ACCGGC [GA]^CCGG[CT]
2288 GCCGGT
EagI 157 CGGCCG C^GGCCG
3557 CGGCCG
EclXI 157 CGGCCG C^GGCCG
3557 CGGCCG
Eco52I 157 CGGCCG C^GGCCG
3557 CGGCCG
EcoNI 2534 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
4306 CCTCAGGGAGG
PvuII 2389 CAGCTG CAG^CTG
2513 CAGCTG
ScaI 687 AGTACT AGT^ACT
2370 AGTACT
SexAI 884 ACCAGGT A^CC[AT]GGT
3067 ACCAGGT
SphI 1470 GCATGC GCATG^C
4620 GCATGC
SspBI 3925 TGTACA T^GTACA
4734 TGTACA
XmnI 393 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
396 GAATTTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 124 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
Alw44I AlwI AlwNI AocI ApaI
ApaLI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoO109I EcoO65I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII XmaCI XmaI