##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII AccII AccIII AcyI AflII
AgeI AhdI Aor51HI AscI AseI
AsnI AspEI AviII AvrII BamHI
BbeI BbrPI BcgI BclI BfrI
BlnI BsaAI BsaHI BseAI BsiEI
BsiWI BspDI BspEI BspLU11I BsrBI
BssHII Bst1107I BstBI BstUI BstZ17I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco47III Eco52I FbaI FseI
FspI Hin1I HindIII KasI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacII SalI SexAI SfoI
SfuI SgrAI SnaBI SpeI SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 2926 GGTACC G^GTACC
AccI 4037 GTAGAC GT^[AC][GT]AC
AclI 1590 AACGTT AA^CGTT
AflIII 850 ACGTGT A^C[GA][CT]GT
Asp718I 2926 GGTACC G^GTACC
BglII 1707 AGATCT A^GATCT
BsaBI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 978 ACCGGA [AT]^CCGG[AT]
BsmI 3271 GCATTC GAATGC(1/-1)
Bsp1407I 3859 TGTACA T^GTACA
BsrDI 4613 GCAATG GCAATG(2/0)
BsrFI 2662 ACCGGC [GA]^CCGG[CT]
BsrGI 3859 TGTACA T^GTACA
BstEII 4574 GGTGACC G^GT[ACGT]ACC
BstPI 4574 GGTGACC G^GT[ACGT]ACC
Cfr10I 2662 ACCGGC [GA]^CCGG[CT]
DraIII 3066 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 4574 GGTGACC G^GT[ACGT]ACC
EcoRV 3437 GATATC GAT^ATC
EcoT22I 3627 ATGCAT ATGCA^T
HpaI 885 GTTAAC GTT^AAC
KpnI 2926 GGTACC GGTAC^C
MamI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NheI 4248 GCTAGC G^CTAGC
NsiI 3627 ATGCAT ATGCA^T
PshAI 3075 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1590 AACGTT AA^CGTT
SbfI 2765 CCTGCAGG CCTGCA^GG
ScaI 5214 AGTACT AGT^ACT
SfiI 1657 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 308 CCCGGG CCC^GGG
Sse8387I 2765 CCTGCAGG CCTGCA^GG
SspBI 3859 TGTACA T^GTACA
XbaI 3426 TCTAGA T^CTAGA
XcmI 4560 CCACAGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 308 CCCGGG C^CCGGG
XmaI 308 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
Alw44I 1060 GTGCAC G^TGCAC
2580 GTGCAC
AocI 2328 CCTCAGG CC^T[ACGT]AGG
3143 CCTCAGG
ApaLI 1060 GTGCAC G^TGCAC
2580 GTGCAC
AspI 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2989 GACCTCGTC
BbsI 1647 GTCTTC GAAGAC(2/6)
4796 GTCTTC
BciVI 4172 GGATAC GTATCC(6/5)
4330 GTATCC
BpuAI 1647 GTCTTC GAAGAC(2/6)
4796 GTCTTC
BsmBI 274 CGTCTC CGTCTC(1/5)
417 GAGACG
BspMI 2520 ACCTGC ACCTGC(4/8)
2764 ACCTGC
BssSI 315 CACGAG CACGAG(-5/-1)
376 CTCGTG
BstAPI 2311 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3571 GCATGCCATGC
Bsu36I 2328 CCTCAGG CC^T[ACGT]AGG
3143 CCTCAGG
DraI 3941 TTTAAA TTT^AAA
5111 TTTAAA
Eco81I 2328 CCTCAGG CC^T[ACGT]AGG
3143 CCTCAGG
FauI 2714 CCCGC CCCGC(4/6)
3268 CCCGC
HincII 885 GTTAAC GT[CT]^[GA]AC
3451 GTTGAC
HindII 885 GTTAAC GT[CT]^[GA]AC
3451 GTTGAC
PflFI 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2989 GACCTCGTC
SacI 1047 GAGCTC GAGCT^C
2645 GAGCTC
SapI 2353 GCTCTTC GCTCTTC(1/4)
3183 GCTCTTC
Tth111I 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
2989 GACCTCGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApaI ApoI Asp700I AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspHI BsrI BssKI BstF5I BstNI
BstXI BstYI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRI
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RcaI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII XmnI