################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AatII AccII AccIII AcyI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AviII AvrII BamHI BbeI BbrPI BcgI BclI BfrI BlnI BsaAI BsaHI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BssHII Bst1107I BstBI BstUI BstZ17I ClaI CpoI DrdI EagI Eam1105I EclXI Eco47III Eco52I FbaI FseI FspI Hin1I HindIII KasI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SexAI SfoI SfuI SgrAI SnaBI SpeI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### Acc65I 2926 GGTACC G^GTACC AccI 4037 GTAGAC GT^[AC][GT]AC AclI 1590 AACGTT AA^CGTT AflIII 850 ACGTGT A^C[GA][CT]GT Asp718I 2926 GGTACC G^GTACC BglII 1707 AGATCT A^GATCT BsaBI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 978 ACCGGA [AT]^CCGG[AT] BsmI 3271 GCATTC GAATGC(1/-1) Bsp1407I 3859 TGTACA T^GTACA BsrDI 4613 GCAATG GCAATG(2/0) BsrFI 2662 ACCGGC [GA]^CCGG[CT] BsrGI 3859 TGTACA T^GTACA BstEII 4574 GGTGACC G^GT[ACGT]ACC BstPI 4574 GGTGACC G^GT[ACGT]ACC Cfr10I 2662 ACCGGC [GA]^CCGG[CT] DraIII 3066 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 4574 GGTGACC G^GT[ACGT]ACC EcoRV 3437 GATATC GAT^ATC EcoT22I 3627 ATGCAT ATGCA^T HpaI 885 GTTAAC GTT^AAC KpnI 2926 GGTACC GGTAC^C MamI 219 GATGCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NheI 4248 GCTAGC G^CTAGC NsiI 3627 ATGCAT ATGCA^T PshAI 3075 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1590 AACGTT AA^CGTT SbfI 2765 CCTGCAGG CCTGCA^GG ScaI 5214 AGTACT AGT^ACT SfiI 1657 GGCCCCAGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 308 CCCGGG CCC^GGG Sse8387I 2765 CCTGCAGG CCTGCA^GG SspBI 3859 TGTACA T^GTACA XbaI 3426 TCTAGA T^CTAGA XcmI 4560 CCACAGCTGCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 308 CCCGGG C^CCGGG XmaI 308 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### Alw44I 1060 GTGCAC G^TGCAC 2580 GTGCAC AocI 2328 CCTCAGG CC^T[ACGT]AGG 3143 CCTCAGG ApaLI 1060 GTGCAC G^TGCAC 2580 GTGCAC AspI 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2989 GACCTCGTC BbsI 1647 GTCTTC GAAGAC(2/6) 4796 GTCTTC BciVI 4172 GGATAC GTATCC(6/5) 4330 GTATCC BpuAI 1647 GTCTTC GAAGAC(2/6) 4796 GTCTTC BsmBI 274 CGTCTC CGTCTC(1/5) 417 GAGACG BspMI 2520 ACCTGC ACCTGC(4/8) 2764 ACCTGC BssSI 315 CACGAG CACGAG(-5/-1) 376 CTCGTG BstAPI 2311 GCAACCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3571 GCATGCCATGC Bsu36I 2328 CCTCAGG CC^T[ACGT]AGG 3143 CCTCAGG DraI 3941 TTTAAA TTT^AAA 5111 TTTAAA Eco81I 2328 CCTCAGG CC^T[ACGT]AGG 3143 CCTCAGG FauI 2714 CCCGC CCCGC(4/6) 3268 CCCGC HincII 885 GTTAAC GT[CT]^[GA]AC 3451 GTTGAC HindII 885 GTTAAC GT[CT]^[GA]AC 3451 GTTGAC PflFI 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2989 GACCTCGTC SacI 1047 GAGCTC GAGCT^C 2645 GAGCTC SapI 2353 GCTCTTC GCTCTTC(1/4) 3183 GCTCTTC Tth111I 419 GACGCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 2989 GACCTCGTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspHI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRI EcoRII EcoT14I Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII XmnI