##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccIII AclI AflII AhdI
Aor51HI AscI AspEI AviII AvrII
BbrPI BclI BfrI BlnI BsaAI
BsaBI BseAI BsiWI BsmBI BspEI
BspHI BsrDI Bst1107I BstBI BstZ17I
CpoI DrdI Eam1105I Eco47III EcoRI
EcoRV FbaI FseI FspI HpaI
KspI MamI MluI MroI NdeI
NheI NotI NruI NspV PacI
PaeR7I PmaCI PmeI PmlI PshAI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
Acc65I 1415 GGTACC G^GTACC
AccI 3734 GTAGAC GT^[AC][GT]AC
AcsI 981 AAATTC [GA]^AATT[CT]
AflIII 194 ACATGT A^C[GA][CT]GT
AgeI 3130 ACCGGT A^CCGGT
Alw44I 35 GTGCAC G^TGCAC
AocI 2765 CCTTAGG CC^T[ACGT]AGG
ApaLI 35 GTGCAC G^TGCAC
ApoI 981 AAATTC [GA]^AATT[CT]
AseI 2266 ATTAAT AT^TAAT
AsnI 2266 ATTAAT AT^TAAT
Asp700I 847 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1415 GGTACC G^GTACC
BalI 3926 TGGCCA TGG^CCA
BamHI 3990 GGATCC G^GATCC
BbeI 2063 GGCGCC GGCGC^C
BsaWI 3130 ACCGGT [AT]^CCGG[AT]
BsmI 2771 GAATGC GAATGC(1/-1)
Bsp1407I 4075 TGTACA T^GTACA
BspDI 2262 ATCGAT AT^CGAT
BspLU11I 194 ACATGT A^CATGT
BsrGI 4075 TGTACA T^GTACA
BssHII 462 GCGCGC G^CGCGC
BstXI 55 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2765 CCTTAGG CC^T[ACGT]AGG
ClaI 2262 ATCGAT AT^CGAT
Eco81I 2765 CCTTAGG CC^T[ACGT]AGG
HincII 481 GTCAAC GT[CT]^[GA]AC
HindII 481 GTCAAC GT[CT]^[GA]AC
HindIII 583 AAGCTT A^AGCTT
KasI 2063 GGCGCC G^GCGCC
KpnI 1415 GGTACC GGTAC^C
MaeII 596 ACGT A^CGT
MfeI 2255 CAATTG C^AATTG
MluNI 3926 TGGCCA TGG^CCA
MscI 3926 TGGCCA TGG^CCA
MunI 2255 CAATTG C^AATTG
NarI 2063 GGCGCC GG^CGCC
NcoI 3923 CCATGG C^CATGG
PinAI 3130 ACCGGT A^CCGGT
PshBI 2266 ATTAAT AT^TAAT
SacI 499 GAGCTC GAGCT^C
SapI 501 GCTCTTC GCTCTTC(1/4)
SexAI 2862 ACCAGGT A^CC[AT]GGT
SfoI 2063 GGCGCC GGC^GCC
SmaI 3381 CCCGGG CCC^GGG
SspBI 4075 TGTACA T^GTACA
XmaCI 3381 CCCGGG C^CCGGG
XmaI 3381 CCCGGG C^CCGGG
XmnI 847 GAAATTATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AcyI 475 GACGCC G[GA]^CG[CT]C
2063 GGCGCC
BcgI 1017 CGATGCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
37 GCACTCGGGTCG
BciVI 1503 GGATAC GTATCC(6/5)
2908 GTATCC
BglII 1996 AGATCT A^GATCT
3592 AGATCT
BsaHI 475 GACGCC G[GA]^CG[CT]C
2063 GGCGCC
BssSI 3033 CACGAG CACGAG(-5/-1)
3823 CACGAG
BstEII 420 GGTGACC G^GT[ACGT]ACC
1979 GGTCACC
BstPI 420 GGTGACC G^GT[ACGT]ACC
1979 GGTCACC
DraIII 2181 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
3823 CACGAGGTG
EagI 106 CGGCCG C^GGCCG
304 CGGCCG
EclXI 106 CGGCCG C^GGCCG
304 CGGCCG
Eco52I 106 CGGCCG C^GGCCG
304 CGGCCG
EcoO65I 420 GGTGACC G^GT[ACGT]ACC
1979 GGTCACC
EcoT14I 370 CCAAGG C^C[AT][AT]GG
3923 CCATGG
EcoT22I 2321 ATGCAT ATGCA^T
2851 ATGCAT
Hin1I 475 GACGCC G[GA]^CG[CT]C
2063 GGCGCC
MspA1I 1003 CAGCTG C[AC]G^C[GT]G
1039 CAGCTG
NaeI 104 GCCGGC GCC^GGC
381 GCCGGC
NgoMIV 104 GCCGGC G^CCGGC
381 GCCGGC
NsiI 2321 ATGCAT ATGCA^T
2851 ATGCAT
PpuMI 2699 AGGTCCC [GA]G^G[AT]CC[CT]
3099 GGGTCCT
PvuII 1003 CAGCTG CAG^CTG
1039 CAGCTG
SphI 1444 GCATGC GCATG^C
2513 GCATGC
StyI 370 CCAAGG C^C[AT][AT]GG
3923 CCATGG
XbaI 535 TCTAGA T^CTAGA
3772 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI ApaI AspHI AspI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsgI BsiEI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrBI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstUI
BstYI Cac8I CelII CfoI Cfr10I
Cfr13I DdeI DpnII DraI DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflFI PflMI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StuI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI Tth111I TthHB8I Van91I XcmI
XhoII