##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
Acc65I AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AviII BciVI
BsaBI BspDI BsrBI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I EcoRI FauI FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MunI NaeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI PvuI RsrII
SacII SalI SapI SfiI SfuI
SgrAI SmaI SwaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 586 GACGTC GACGT^C
AccIII 1367 TCCGGA T^CCGGA
AflIII 3116 ACATGT A^C[GA][CT]GT
AhdI 2422 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1494 GGGCCC GGGCC^C
Asp700I 3079 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 2422 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 3777 CTCGGG C^[CT]CG[GA]G
AvrII 2653 CCTAGG C^CTAGG
BbeI 33 GGCGCC GGCGC^C
BbrPI 4366 CACGTG CAC^GTG
BbvCI 3373 CCTCAGC CCTCAGC(-5/-2)
BcgI 361 CGACCCCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 5095 AGATCT A^GATCT
BlnI 2653 CCTAGG C^CTAGG
BlpI 2108 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2108 GCTCAGC GC^T[ACGT]AGC
BseAI 1367 TCCGGA T^CCGGA
BsiEI 887 CGGTCG CG[GA][CT]^CG
BsiWI 392 CGTACG C^GTACG
BsmBI 1685 GAGACG CGTCTC(1/5)
BsoBI 3777 CTCGGG C^[CT]CG[GA]G
BspEI 1367 TCCGGA T^CCGGA
BspHI 2482 TCATGA T^CATGA
BspLU11I 3116 ACATGT A^CATGT
CelII 2108 GCTCAGC GC^T[ACGT]AGC
Eam1105I 2422 GACCTTCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRV 1641 GATATC GAT^ATC
HindIII 3585 AAGCTT A^AGCTT
KasI 33 GGCGCC G^GCGCC
MroI 1367 TCCGGA T^CCGGA
NarI 33 GGCGCC GG^CGCC
NdeI 2231 CATATG CA^TATG
PmaCI 4366 CACGTG CAC^GTG
PmlI 4366 CACGTG CAC^GTG
PshAI 310 GACAAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 2482 TCATGA T^CATGA
SbfI 5023 CCTGCAGG CCTGCA^GG
SexAI 1412 ACCAGGT A^CC[AT]GGT
SfoI 33 GGCGCC GGC^GCC
SnaBI 390 TACGTA TAC^GTA
Sse8387I 5023 CCTGCAGG CCTGCA^GG
SspI 1038 AATATT AAT^ATT
TaqI 4162 TCGA T^CGA
TthHB8I 4162 TCGA T^CGA
XbaI 867 TCTAGA T^CTAGA
XcmI 2087 CCAGGCAGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3079 GAACCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 583 GTAGAC GT^[AC][GT]AC
3153 GTAGAC
AccII 3 CGCG CG^CG
5 CGCG
AciI 13 CCGC CCGC(-3/-1)
6 GCGG
AclI 1456 AACGTT AA^CGTT
3919 AACGTT
AcsI 1862 AAATTC [GA]^AATT[CT]
2563 AAATTT
Alw44I 3674 GTGCAC G^TGCAC
4101 GTGCAC
ApaLI 3674 GTGCAC G^TGCAC
4101 GTGCAC
ApoI 1862 AAATTC [GA]^AATT[CT]
2563 AAATTT
AspI 512 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACCAAGTC
BamHI 2193 GGATCC G^GATCC
3432 GGATCC
BclI 2024 TGATCA T^GATCA
2579 TGATCA
BsaWI 618 TCCGGT [AT]^CCGG[AT]
1367 TCCGGA
Bsp1407I 2843 TGTACA T^GTACA
3119 TGTACA
BsrFI 686 ACCGGC [GA]^CCGG[CT]
5138 ACCGGC
BsrGI 2843 TGTACA T^GTACA
3119 TGTACA
BstUI 3 CGCG CG^CG
5 CGCG
Cfr10I 686 ACCGGC [GA]^CCGG[CT]
5138 ACCGGC
DraIII 186 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
4741 CACACTGTG
EarI 2044 CTCTTC CTCTTC(1/4)
3011 CTCTTC
EcoT22I 2245 ATGCAT ATGCA^T
3287 ATGCAT
FbaI 2024 TGATCA T^GATCA
2579 TGATCA
HincII 610 GTTGAC GT[CT]^[GA]AC
3355 GTCAAC
HindII 610 GTTGAC GT[CT]^[GA]AC
3355 GTCAAC
Ksp632I 2044 CTCTTC CTCTTC(1/4)
3011 CTCTTC
MspA1I 1058 CAGCTG C[AC]G^C[GT]G
1516 CAGCTG
MvnI 3 CGCG CG^CG
5 CGCG
NsiI 2245 ATGCAT ATGCA^T
3287 ATGCAT
PflFI 512 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACCAAGTC
Psp1406I 1456 AACGTT AA^CGTT
3919 AACGTT
PvuII 1058 CAGCTG CAG^CTG
1516 CAGCTG
SacI 2372 GAGCTC GAGCT^C
3774 GAGCTC
SpeI 1898 ACTAGT A^CTAGT
3496 ACTAGT
SphI 2968 GCATGC GCATG^C
3285 GCATGC
SspBI 2843 TGTACA T^GTACA
3119 TGTACA
Tth111I 512 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
3556 GACCAAGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 109 enzymes) #
#########################################
AcyI AfaI AflII AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbsI BbvI BcnI BfaI
BfrI BglI BmyI BpmI BpuAI
BsaAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I BspMI BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PflMI PleI PpuMI
PstI RsaI Sau3AI Sau96I ScaI
ScrFI SfaNI SfcI SmlI StuI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI Van91I XhoII