##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AviII BamHI BbeI
BglII BsiEI BsiWI BsmI BspDI
BssHII Bst1107I BstBI BstZ17I Bsu36I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I Eco81I EcoRI EcoT22I
FseI FspI HpaI KasI KpnI
KspI MfeI MluI MunI NarI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI Sse8387I
SwaI Tth111I XbaI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AflII 4265 CTTAAG C^TTAAG
AhdI 1711 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2395 GTGCAC G^TGCAC
ApaLI 2395 GTGCAC G^TGCAC
Asp700I 2099 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1711 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 2222 CCTAGG C^CTAGG
BbrPI 2131 CACGTG CAC^GTG
BbvCI 4390 GCTGAGG CCTCAGC(-5/-2)
BcnI 3624 CCGGG CC^[GC]GG
BfrI 4265 CTTAAG C^TTAAG
BlnI 2222 CCTAGG C^CTAGG
BlpI 851 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 851 GCTGAGC GC^T[ACGT]AGC
BsaAI 2131 CACGTG [CT]AC^GT[GA]
BsaBI 4202 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
Bsp1407I 743 TGTACA T^GTACA
BspMI 2733 GCAGGT ACCTGC(4/8)
BsrGI 743 TGTACA T^GTACA
CelII 851 GCTGAGC GC^T[ACGT]AGC
DraIII 920 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 3767 GACAGATATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1711 GACTGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HaeII 22 GGCGCT [GA]GCGC^[CT]
HincII 929 GTTGAC GT[CT]^[GA]AC
HindII 929 GTTGAC GT[CT]^[GA]AC
MaeII 2132 ACGT A^CGT
MamI 4202 GATGTGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 3624 CCGGG CC^[GC]GG
NdeI 867 CATATG CA^TATG
NheI 2038 GCTAGC G^CTAGC
PmaCI 2131 CACGTG CAC^GTG
PmlI 2131 CACGTG CAC^GTG
SacI 1765 GAGCTC GAGCT^C
ScaI 3285 AGTACT AGT^ACT
SphI 573 GCATGC GCATG^C
SspBI 743 TGTACA T^GTACA
SspI 3162 AATATT AAT^ATT
XmnI 2099 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 19 CGCG CG^CG
47 CGCG
AccIII 419 TCCGGA T^CCGGA
2531 TCCGGA
AcyI 889 GACGCC G[GA]^CG[CT]C
1505 GGCGTC
AvaI 434 CTCGGG C^[CT]CG[GA]G
3633 CCCGAG
BcgI 426 GCAGCAACCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1950 GCAATGGAGTCG
BciVI 2862 GGATAC GTATCC(6/5)
2942 GTATCC
BglI 2065 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3043 GCCCTGATGGC
BsaHI 889 GACGCC G[GA]^CG[CT]C
1505 GGCGTC
BsaWI 419 TCCGGA [AT]^CCGG[AT]
2531 TCCGGA
BseAI 419 TCCGGA T^CCGGA
2531 TCCGGA
BsmBI 1318 CGTCTC CGTCTC(1/5)
4284 GAGACG
BsoBI 434 CTCGGG C^[CT]CG[GA]G
3633 CCCGAG
BspEI 419 TCCGGA T^CCGGA
2531 TCCGGA
BspHI 2423 TCATGA T^CATGA
4253 TCATGA
BspLU11I 1104 ACATGT A^CATGT
1432 ACATGT
BsrBI 1165 CCGCTC CCGCTC(-3/-3)
1328 GAGCGG
BssSI 994 CACGAG CACGAG(-5/-1)
2163 CTCGTG
BstEII 1262 GGTGACC G^GT[ACGT]ACC
3493 GGTGACC
BstPI 1262 GGTGACC G^GT[ACGT]ACC
3493 GGTGACC
BstUI 19 CGCG CG^CG
47 CGCG
BstYI 499 GGATCT [GA]^GATC[CT]
3402 GGATCT
EcoO65I 1262 GGTGACC G^GT[ACGT]ACC
3493 GGTGACC
EcoRV 1639 GATATC GAT^ATC
4052 GATATC
Hin1I 889 GACGCC G[GA]^CG[CT]C
1505 GGCGTC
MflI 499 GGATCT [GA]^GATC[CT]
3402 GGATCT
MroI 419 TCCGGA T^CCGGA
2531 TCCGGA
MvnI 19 CGCG CG^CG
47 CGCG
NcoI 1227 CCATGG C^CATGG
2764 CCATGG
RcaI 2423 TCATGA T^CATGA
4253 TCATGA
SexAI 921 ACCAGGT A^CC[AT]GGT
2563 ACCAGGT
XhoII 499 GGATCT [GA]^GATC[CT]
3402 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BclI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrDI BsrFI BsrI BssKI
BstAPI BstF5I BstNI BstXI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI FbaI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NaeI NdeII NgoMIV NlaIII
NlaIV NspI PflMI PleI PpuMI
PshAI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI