################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AatII AccI AclI AflII Alw44I ApaLI AscI AseI AsnI AspI AviII AvrII BbeI BcgI BciVI BfrI BglI BlnI BsiEI BsiWI BspDI BspHI BssHII Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV FseI FspI HincII HindII HpaI KasI KspI MluI NarI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### Acc65I 2520 GGTACC G^GTACC AccIII 3378 TCCGGA T^CCGGA AcyI 501 GACGCC G[GA]^CG[CT]C AgeI 2664 ACCGGT A^CCGGT AhdI 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Aor51HI 1292 AGCGCT AGC^GCT Asp718I 2520 GGTACC G^GTACC AspEI 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 720 TGGCCA TGG^CCA BglII 2952 AGATCT A^GATCT BlpI 3844 GCTAAGC GC^T[ACGT]AGC Bpu1102I 3844 GCTAAGC GC^T[ACGT]AGC BsaBI 1126 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 501 GACGCC G[GA]^CG[CT]C BsaI 2039 GAGACC GGTCTC(1/5) BseAI 3378 TCCGGA T^CCGGA BspEI 3378 TCCGGA T^CCGGA BspLU11I 576 ACATGT A^CATGT BsrBI 479 GAGCGG CCGCTC(-3/-3) BssSI 3512 CACGAG CACGAG(-5/-1) BstAPI 1264 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 1546 GGTGACC G^GT[ACGT]ACC BstPI 1546 GGTGACC G^GT[ACGT]ACC CelII 3844 GCTAAGC GC^T[ACGT]AGC Eam1105I 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1292 AGCGCT AGC^GCT EcoO65I 1546 GGTGACC G^GT[ACGT]ACC EcoT22I 228 ATGCAT ATGCA^T Hin1I 501 GACGCC G[GA]^CG[CT]C HindIII 3953 AAGCTT A^AGCTT KpnI 2520 GGTACC GGTAC^C MamI 1126 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 720 TGGCCA TGG^CCA MroI 3378 TCCGGA T^CCGGA MscI 720 TGGCCA TGG^CCA NcoI 1971 CCATGG C^CATGG NdeI 1145 CATATG CA^TATG NsiI 228 ATGCAT ATGCA^T PflMI 995 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PinAI 2664 ACCGGT A^CCGGT SacI 785 GAGCTC GAGCT^C SbfI 1459 CCTGCAGG CCTGCA^GG SnaBI 663 TACGTA TAC^GTA SpeI 4036 ACTAGT A^CTAGT SphI 3148 GCATGC GCATG^C Sse8387I 1459 CCTGCAGG CCTGCA^GG SspI 4079 AATATT AAT^ATT StuI 1456 AGGCCT AGG^CCT Van91I 995 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2416 TCTAGA T^CTAGA XcmI 2734 CCAGTATTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AflIII 576 ACATGT A^C[GA][CT]GT 2571 ACGTGT AocI 359 CCTCAGG CC^T[ACGT]AGG 1552 CCTCAGG AspHI 785 GAGCTC G[AT]GC[AT]^C 3775 GAGCAC BanI 1717 GGTGCC G^G[CT][GA]CC 2520 GGTACC BbvCI 1373 GCTGAGG CCTCAGC(-5/-2) 3837 GCTGAGG BsiHKAI 785 GAGCTC G[AT]GC[AT]^C 3775 GAGCAC BsmI 2267 GAATGC GAATGC(1/-1) 3495 GCATTC Bsu36I 359 CCTCAGG CC^T[ACGT]AGG 1552 CCTCAGG DraIII 1722 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG 2567 CACAACGTG DrdI 501 GACGCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1075 GACCTGCGCGTC DsaI 411 CCGTGG C^C[GA][CT]GG 1971 CCATGG EaeI 720 TGGCCA [CT]^GGCC[GA] 1204 TGGCCG Eco81I 359 CCTCAGG CC^T[ACGT]AGG 1552 CCTCAGG HaeII 1292 AGCGCT [GA]GCGC^[CT] 3594 GGCGCT MfeI 2427 CAATTG C^AATTG 3297 CAATTG MunI 2427 CAATTG C^AATTG 3297 CAATTG ScaI 1344 AGTACT AGT^ACT 3750 AGTACT SmaI 38 CCCGGG CCC^GGG 348 CCCGGG XmaCI 38 CCCGGG C^CCGGG 348 CCCGGG XmaI 38 CCCGGG C^CCGGG 348 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 120 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI Asp700I AvaI AvaII BamHI BanII BbrPI BbsI BbvI BclI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsaWI BseRI BsgI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I Bsp1407I BspMI BsrDI BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EarI EcoNI EcoO109I EcoRII EcoT14I FauI FbaI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI