##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AatII AccI AclI AflII Alw44I
ApaLI AscI AseI AsnI AspI
AviII AvrII BbeI BcgI BciVI
BfrI BglI BlnI BsiEI BsiWI
BspDI BspHI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DraI EagI
EclXI Eco52I EcoRI EcoRV FseI
FspI HincII HindII HpaI KasI
KspI MluI NarI NheI NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SwaI
Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 2520 GGTACC G^GTACC
AccIII 3378 TCCGGA T^CCGGA
AcyI 501 GACGCC G[GA]^CG[CT]C
AgeI 2664 ACCGGT A^CCGGT
AhdI 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1292 AGCGCT AGC^GCT
Asp718I 2520 GGTACC G^GTACC
AspEI 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 720 TGGCCA TGG^CCA
BglII 2952 AGATCT A^GATCT
BlpI 3844 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 3844 GCTAAGC GC^T[ACGT]AGC
BsaBI 1126 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 501 GACGCC G[GA]^CG[CT]C
BsaI 2039 GAGACC GGTCTC(1/5)
BseAI 3378 TCCGGA T^CCGGA
BspEI 3378 TCCGGA T^CCGGA
BspLU11I 576 ACATGT A^CATGT
BsrBI 479 GAGCGG CCGCTC(-3/-3)
BssSI 3512 CACGAG CACGAG(-5/-1)
BstAPI 1264 GCAGGGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1546 GGTGACC G^GT[ACGT]ACC
BstPI 1546 GGTGACC G^GT[ACGT]ACC
CelII 3844 GCTAAGC GC^T[ACGT]AGC
Eam1105I 104 GACGCACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1292 AGCGCT AGC^GCT
EcoO65I 1546 GGTGACC G^GT[ACGT]ACC
EcoT22I 228 ATGCAT ATGCA^T
Hin1I 501 GACGCC G[GA]^CG[CT]C
HindIII 3953 AAGCTT A^AGCTT
KpnI 2520 GGTACC GGTAC^C
MamI 1126 GATCCAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 720 TGGCCA TGG^CCA
MroI 3378 TCCGGA T^CCGGA
MscI 720 TGGCCA TGG^CCA
NcoI 1971 CCATGG C^CATGG
NdeI 1145 CATATG CA^TATG
NsiI 228 ATGCAT ATGCA^T
PflMI 995 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2664 ACCGGT A^CCGGT
SacI 785 GAGCTC GAGCT^C
SbfI 1459 CCTGCAGG CCTGCA^GG
SnaBI 663 TACGTA TAC^GTA
SpeI 4036 ACTAGT A^CTAGT
SphI 3148 GCATGC GCATG^C
Sse8387I 1459 CCTGCAGG CCTGCA^GG
SspI 4079 AATATT AAT^ATT
StuI 1456 AGGCCT AGG^CCT
Van91I 995 CCACTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2416 TCTAGA T^CTAGA
XcmI 2734 CCAGTATTTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 576 ACATGT A^C[GA][CT]GT
2571 ACGTGT
AocI 359 CCTCAGG CC^T[ACGT]AGG
1552 CCTCAGG
AspHI 785 GAGCTC G[AT]GC[AT]^C
3775 GAGCAC
BanI 1717 GGTGCC G^G[CT][GA]CC
2520 GGTACC
BbvCI 1373 GCTGAGG CCTCAGC(-5/-2)
3837 GCTGAGG
BsiHKAI 785 GAGCTC G[AT]GC[AT]^C
3775 GAGCAC
BsmI 2267 GAATGC GAATGC(1/-1)
3495 GCATTC
Bsu36I 359 CCTCAGG CC^T[ACGT]AGG
1552 CCTCAGG
DraIII 1722 CACGCCGTG CAC[ACGT][ACGT][ACGT]^GTG
2567 CACAACGTG
DrdI 501 GACGCCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1075 GACCTGCGCGTC
DsaI 411 CCGTGG C^C[GA][CT]GG
1971 CCATGG
EaeI 720 TGGCCA [CT]^GGCC[GA]
1204 TGGCCG
Eco81I 359 CCTCAGG CC^T[ACGT]AGG
1552 CCTCAGG
HaeII 1292 AGCGCT [GA]GCGC^[CT]
3594 GGCGCT
MfeI 2427 CAATTG C^AATTG
3297 CAATTG
MunI 2427 CAATTG C^AATTG
3297 CAATTG
ScaI 1344 AGTACT AGT^ACT
3750 AGTACT
SmaI 38 CCCGGG CCC^GGG
348 CCCGGG
XmaCI 38 CCCGGG C^CCGGG
348 CCCGGG
XmaI 38 CCCGGG C^CCGGG
348 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 120 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI Asp700I
AvaI AvaII BamHI BanII BbrPI
BbsI BbvI BclI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BsaWI BseRI BsgI BsiYI BslI
BsmAI BsmBI BsmFI BsoBI Bsp1286I
Bsp1407I BspMI BsrDI BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstXI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII EarI
EcoNI EcoO109I EcoRII EcoT14I FauI
FbaI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI SspBI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI