################################## # NO CUT ENZYMES ( 91 enzymes) # ################################## AatII AccIII AclI AcsI AflII AgeI Aor51HI ApoI AscI AseI AsnI Asp700I AspI AviII BbeI BbrPI BfrI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspHI BsrBI BsrFI BssHII Bst1107I BstBI BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HindIII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspI SwaI Tth111I XbaI XmnI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### Acc65I 2008 GGTACC G^GTACC AccI 3079 GTCTAC GT^[AC][GT]AC AcyI 2248 GGCGTC G[GA]^CG[CT]C Alw44I 3104 GTGCAC G^TGCAC ApaLI 3104 GTGCAC G^TGCAC Asp718I 2008 GGTACC G^GTACC AvrII 412 CCTAGG C^CTAGG BcgI 2208 CGAGGTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1464 TGATCA T^GATCA BglII 1638 AGATCT A^GATCT BlnI 412 CCTAGG C^CTAGG BlpI 43 GCTGAGC GC^T[ACGT]AGC Bpu1102I 43 GCTGAGC GC^T[ACGT]AGC BsaAI 4033 TACGTG [CT]AC^GT[GA] BsaBI 3954 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaHI 2248 GGCGTC G[GA]^CG[CT]C BsaWI 2343 ACCGGA [AT]^CCGG[AT] BspLU11I 2750 ACATGT A^CATGT BssSI 4223 CTCGTG CACGAG(-5/-1) CelII 43 GCTGAGC GC^T[ACGT]AGC FbaI 1464 TGATCA T^GATCA HaeII 40 GGCGCT [GA]GCGC^[CT] Hin1I 2248 GGCGTC G[GA]^CG[CT]C KpnI 2008 GGTACC GGTAC^C MamI 3954 GATCACCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MseI 3182 TTAA T^TAA NdeI 1982 CATATG CA^TATG PflMI 3117 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ScaI 1896 AGTACT AGT^ACT Tru9I 3182 TTAA T^TAA Van91I 3117 CCAGCATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 23 enzymes) # ################################### AflIII 9 ACACGT A^C[GA][CT]GT 2750 ACATGT AhdI 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1111 GACAGCACGTC AspEI 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1111 GACAGCACGTC BalI 424 TGGCCA TGG^CCA 917 TGGCCA BamHI 76 GGATCC G^GATCC 3427 GGATCC BciVI 1555 GTATCC GTATCC(6/5) 3176 GTATCC BsaI 1085 GAGACC GGTCTC(1/5) 3859 GAGACC BsgI 463 CTGCAC GTGCAG(16/14) 2756 CTGCAC Bsp1407I 1259 TGTACA T^GTACA 3015 TGTACA BspMI 3212 ACCTGC ACCTGC(4/8) 3098 GCAGGT BsrGI 1259 TGTACA T^GTACA 3015 TGTACA BstAPI 103 GCAGCTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2926 GCACATTCTGC DraIII 1934 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG 2718 CACATCGTG Eam1105I 523 GACCTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1111 GACAGCACGTC HincII 1534 GTTGAC GT[CT]^[GA]AC 2307 GTCAAC HindII 1534 GTTGAC GT[CT]^[GA]AC 2307 GTCAAC MluNI 424 TGGCCA TGG^CCA 917 TGGCCA MscI 424 TGGCCA TGG^CCA 917 TGGCCA NcoI 144 CCATGG C^CATGG 588 CCATGG SapI 872 GCTCTTC GCTCTTC(1/4) 1564 GCTCTTC SspBI 1259 TGTACA T^GTACA 3015 TGTACA Tsp509I 1632 AATT ^AATT 2534 AATT XcmI 2717 CCACATCGTGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2993 CCAGGCTACCTGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI StuI StyI TaqI TfiI TseI Tsp45I TspRI TthHB8I XhoI XhoII XmaCI XmaI