##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
Alw44I ApaLI AscI Asp718I AspI
BamHI BbsI BfrI BglII BpuAI
BsaBI BseAI BsiWI Bsp1407I BspDI
BspEI BspHI BsrBI BsrGI BstBI
BstEII BstPI ClaI CpoI DrdI
EagI EclXI Eco52I EcoO65I EcoRI
FseI HindIII HpaI KpnI MamI
MfeI MluI MroI MunI NdeI
NheI NotI NruI NspV PacI
PaeR7I PflFI PmeI PshAI Psp1406I
RcaI RsrII SalI SbfI SexAI
SfuI SgrAI SnaBI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AcsI 2148 GAATTT [GA]^AATT[CT]
AgeI 459 ACCGGT A^CCGGT
AocI 2139 CCTAAGG CC^T[ACGT]AGG
Aor51HI 820 AGCGCT AGC^GCT
ApaI 56 GGGCCC GGGCC^C
ApoI 2148 GAATTT [GA]^AATT[CT]
AseI 1964 ATTAAT AT^TAAT
AsnI 1964 ATTAAT AT^TAAT
Asp700I 866 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 452 TGCGCA TGC^GCA
BanI 573 GGCGCC G^G[CT][GA]CC
BbeI 573 GGCGCC GGCGC^C
BbrPI 1221 CACGTG CAC^GTG
BcgI 155 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 144 GGATAC GTATCC(6/5)
BclI 2667 TGATCA T^GATCA
BlpI 36 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC
BsaWI 459 ACCGGT [AT]^CCGG[AT]
BsiEI 126 CGATCG CG[GA][CT]^CG
BsmBI 915 GAGACG CGTCTC(1/5)
BspLU11I 1595 ACATGT A^CATGT
BssSI 2600 CTCGTG CACGAG(-5/-1)
Bst1107I 1512 GTATAC GTA^TAC
BstXI 1289 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1512 GTATAC GTA^TAC
Bsu36I 2139 CCTAAGG CC^T[ACGT]AGG
CelII 36 GCTGAGC GC^T[ACGT]AGC
DraI 2592 TTTAAA TTT^AAA
Eco47III 820 AGCGCT AGC^GCT
Eco81I 2139 CCTAAGG CC^T[ACGT]AGG
EcoNI 608 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 1785 GATATC GAT^ATC
EcoT22I 2281 ATGCAT ATGCA^T
FbaI 2667 TGATCA T^GATCA
FspI 452 TGCGCA TGC^GCA
HincII 2546 GTTGAC GT[CT]^[GA]AC
HindII 2546 GTTGAC GT[CT]^[GA]AC
KasI 573 GGCGCC G^GCGCC
KspI 212 CCGCGG CCGC^GG
NaeI 576 GCCGGC GCC^GGC
NarI 573 GGCGCC GG^CGCC
NcoI 1956 CCATGG C^CATGG
NgoMIV 576 GCCGGC G^CCGGC
NsiI 2281 ATGCAT ATGCA^T
PinAI 459 ACCGGT A^CCGGT
PmaCI 1221 CACGTG CAC^GTG
PmlI 1221 CACGTG CAC^GTG
PshBI 1964 ATTAAT AT^TAAT
PvuI 126 CGATCG CGAT^CG
PvuII 2519 CAGCTG CAG^CTG
SacII 212 CCGCGG CCGC^GG
SfiI 57 GGCCCGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 573 GGCGCC GGC^GCC
SmaI 179 CCCGGG CCC^GGG
SpeI 1092 ACTAGT A^CTAGT
SphI 2279 GCATGC GCATG^C
XcmI 598 CCACGCTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 179 CCCGGG C^CCGGG
XmaI 179 CCCGGG C^CCGGG
XmnI 866 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AccI 1512 GTATAC GT^[AC][GT]AC
2958 GTCTAC
AflIII 403 ACGTGT A^C[GA][CT]GT
1595 ACATGT
AvrII 2287 CCTAGG C^CTAGG
2372 CCTAGG
BalI 1127 TGGCCA TGG^CCA
1959 TGGCCA
BbvCI 116 CCTCAGC CCTCAGC(-5/-2)
2295 CCTCAGC
BglI 58 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1380 GCCTCTGGGGC
BlnI 2287 CCTAGG C^CTAGG
2372 CCTAGG
BsaI 435 GAGACC GGTCTC(1/5)
2211 GAGACC
BspMI 535 ACCTGC ACCTGC(4/8)
1213 GCAGGT
BsrFI 459 ACCGGT [GA]^CCGG[CT]
576 GCCGGC
BstAPI 737 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2009 GCAGCTCCTGC
Cfr10I 459 ACCGGT [GA]^CCGG[CT]
576 GCCGGC
DraIII 246 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG
2070 CACACAGTG
DsaI 212 CCGCGG C^C[GA][CT]GG
1956 CCATGG
MluNI 1127 TGGCCA TGG^CCA
1959 TGGCCA
MscI 1127 TGGCCA TGG^CCA
1959 TGGCCA
MslI 490 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2216 CATAAAAATG
PflMI 1377 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCACACAGTGG
SapI 897 GAAGAGC GCTCTTC(1/4)
2001 GAAGAGC
ScaI 2844 AGTACT AGT^ACT
2877 AGTACT
Van91I 1377 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2069 CCACACAGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcyI AfaI AhdI
AluI AlwI AlwNI AspEI AspHI
AvaI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI BsoBI
Bsp1286I BsrDI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI Eam1105I EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII