################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AccIII AclI AflII Alw44I ApaLI AscI Asp718I AspI BamHI BbsI BfrI BglII BpuAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrBI BsrGI BstBI BstEII BstPI ClaI CpoI DrdI EagI EclXI Eco52I EcoO65I EcoRI FseI HindIII HpaI KpnI MamI MfeI MluI MroI MunI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PshAI Psp1406I RcaI RsrII SalI SbfI SexAI SfuI SgrAI SnaBI Sse8387I SspBI SspI StuI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### AcsI 2148 GAATTT [GA]^AATT[CT] AgeI 459 ACCGGT A^CCGGT AocI 2139 CCTAAGG CC^T[ACGT]AGG Aor51HI 820 AGCGCT AGC^GCT ApaI 56 GGGCCC GGGCC^C ApoI 2148 GAATTT [GA]^AATT[CT] AseI 1964 ATTAAT AT^TAAT AsnI 1964 ATTAAT AT^TAAT Asp700I 866 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AviII 452 TGCGCA TGC^GCA BanI 573 GGCGCC G^G[CT][GA]CC BbeI 573 GGCGCC GGCGC^C BbrPI 1221 CACGTG CAC^GTG BcgI 155 CGACCCCTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 144 GGATAC GTATCC(6/5) BclI 2667 TGATCA T^GATCA BlpI 36 GCTGAGC GC^T[ACGT]AGC Bpu1102I 36 GCTGAGC GC^T[ACGT]AGC BsaWI 459 ACCGGT [AT]^CCGG[AT] BsiEI 126 CGATCG CG[GA][CT]^CG BsmBI 915 GAGACG CGTCTC(1/5) BspLU11I 1595 ACATGT A^CATGT BssSI 2600 CTCGTG CACGAG(-5/-1) Bst1107I 1512 GTATAC GTA^TAC BstXI 1289 CCAGTGGGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1512 GTATAC GTA^TAC Bsu36I 2139 CCTAAGG CC^T[ACGT]AGG CelII 36 GCTGAGC GC^T[ACGT]AGC DraI 2592 TTTAAA TTT^AAA Eco47III 820 AGCGCT AGC^GCT Eco81I 2139 CCTAAGG CC^T[ACGT]AGG EcoNI 608 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRV 1785 GATATC GAT^ATC EcoT22I 2281 ATGCAT ATGCA^T FbaI 2667 TGATCA T^GATCA FspI 452 TGCGCA TGC^GCA HincII 2546 GTTGAC GT[CT]^[GA]AC HindII 2546 GTTGAC GT[CT]^[GA]AC KasI 573 GGCGCC G^GCGCC KspI 212 CCGCGG CCGC^GG NaeI 576 GCCGGC GCC^GGC NarI 573 GGCGCC GG^CGCC NcoI 1956 CCATGG C^CATGG NgoMIV 576 GCCGGC G^CCGGC NsiI 2281 ATGCAT ATGCA^T PinAI 459 ACCGGT A^CCGGT PmaCI 1221 CACGTG CAC^GTG PmlI 1221 CACGTG CAC^GTG PshBI 1964 ATTAAT AT^TAAT PvuI 126 CGATCG CGAT^CG PvuII 2519 CAGCTG CAG^CTG SacII 212 CCGCGG CCGC^GG SfiI 57 GGCCCGCGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfoI 573 GGCGCC GGC^GCC SmaI 179 CCCGGG CCC^GGG SpeI 1092 ACTAGT A^CTAGT SphI 2279 GCATGC GCATG^C XcmI 598 CCACGCTGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 179 CCCGGG C^CCGGG XmaI 179 CCCGGG C^CCGGG XmnI 866 GAACGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AccI 1512 GTATAC GT^[AC][GT]AC 2958 GTCTAC AflIII 403 ACGTGT A^C[GA][CT]GT 1595 ACATGT AvrII 2287 CCTAGG C^CTAGG 2372 CCTAGG BalI 1127 TGGCCA TGG^CCA 1959 TGGCCA BbvCI 116 CCTCAGC CCTCAGC(-5/-2) 2295 CCTCAGC BglI 58 GCCCGCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1380 GCCTCTGGGGC BlnI 2287 CCTAGG C^CTAGG 2372 CCTAGG BsaI 435 GAGACC GGTCTC(1/5) 2211 GAGACC BspMI 535 ACCTGC ACCTGC(4/8) 1213 GCAGGT BsrFI 459 ACCGGT [GA]^CCGG[CT] 576 GCCGGC BstAPI 737 GCAGATCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2009 GCAGCTCCTGC Cfr10I 459 ACCGGT [GA]^CCGG[CT] 576 GCCGGC DraIII 246 CACTGCGTG CAC[ACGT][ACGT][ACGT]^GTG 2070 CACACAGTG DsaI 212 CCGCGG C^C[GA][CT]GG 1956 CCATGG MluNI 1127 TGGCCA TGG^CCA 1959 TGGCCA MscI 1127 TGGCCA TGG^CCA 1959 TGGCCA MslI 490 CATCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2216 CATAAAAATG PflMI 1377 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2069 CCACACAGTGG SapI 897 GAAGAGC GCTCTTC(1/4) 2001 GAAGAGC ScaI 2844 AGTACT AGT^ACT 2877 AGTACT Van91I 1377 CCAGCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2069 CCACACAGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcyI AfaI AhdI AluI AlwI AlwNI AspEI AspHI AvaI AvaII BanII BbvI BcnI BfaI BmyI BpmI BsaAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BsrDI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI Eam1105I EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII