##################################
# NO CUT ENZYMES ( 108 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI AhdI Aor51HI ApaI AscI
AseI AsnI AspEI AspI AvaI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BglI BlnI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsoBI BspDI BspEI
BsrBI BsrFI BssHII BssSI Bst1107I
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FauI
FbaI FseI FspI HaeII HgaI
Hin1I HpaI KasI KspI MamI
MluI MroI MvnI NaeI NarI
NdeI NgoMIV NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
Acc65I 82 GGTACC G^GTACC
AflIII 117 ACATGT A^C[GA][CT]GT
Alw44I 3064 GTGCAC G^TGCAC
ApaLI 3064 GTGCAC G^TGCAC
Asp700I 3200 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 82 GGTACC G^GTACC
BcnI 2589 CCGGG CC^[GC]GG
BglII 1744 AGATCT A^GATCT
BsaAI 280 TACGTG [CT]AC^GT[GA]
BsmI 2199 GCATTC GAATGC(1/-1)
BspHI 3500 TCATGA T^CATGA
BspLU11I 117 ACATGT A^CATGT
BstEII 783 GGTGACC G^GT[ACGT]ACC
BstPI 783 GGTGACC G^GT[ACGT]ACC
CfoI 2601 GCGC GCG^C
DraI 2563 TTTAAA TTT^AAA
DraIII 640 CACAAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 744 GACCTGGAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 783 GGTGACC G^GT[ACGT]ACC
HapII 2589 CCGG C^CGG
HhaI 2601 GCGC GCG^C
HinP1I 2601 GCGC G^CGC
HpaII 2589 CCGG C^CGG
KpnI 82 GGTACC GGTAC^C
MfeI 497 CAATTG C^AATTG
MspI 2589 CCGG C^CGG
MunI 497 CAATTG C^AATTG
NciI 2589 CCGGG CC^[GC]GG
RcaI 3500 TCATGA T^CATGA
SexAI 2180 ACCTGGT A^CC[AT]GGT
SspI 2249 AATATT AAT^ATT
XmnI 3200 GAAAGGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AccI 2669 GTCTAC GT^[AC][GT]AC
3511 GTCTAC
AocI 1024 CCTAAGG CC^T[ACGT]AGG
1857 CCTGAGG
BbvCI 1996 CCTCAGC CCTCAGC(-5/-2)
667 GCTGAGG
Bsp1407I 818 TGTACA T^GTACA
1340 TGTACA
BsrDI 1103 GCAATG GCAATG(2/0)
3576 GCAATG
BsrGI 818 TGTACA T^GTACA
1340 TGTACA
Bsu36I 1024 CCTAAGG CC^T[ACGT]AGG
1857 CCTGAGG
Eco81I 1024 CCTAAGG CC^T[ACGT]AGG
1857 CCTGAGG
HindIII 1303 AAGCTT A^AGCTT
3493 AAGCTT
MaeII 281 ACGT A^CGT
888 ACGT
PflMI 1070 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2617 CCAGCACTTGG
SacI 390 GAGCTC GAGCT^C
2110 GAGCTC
SspBI 818 TGTACA T^GTACA
1340 TGTACA
StuI 249 AGGCCT AGG^CCT
358 AGGCCT
TaqI 246 TCGA T^CGA
1370 TCGA
TfiI 2118 GATTC G^A[AT]TC
3205 GATTC
TthHB8I 246 TCGA T^CGA
1370 TCGA
Van91I 1070 CCAGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2617 CCAGCACTTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AciI AcsI AfaI AflII AluI
AlwI AlwNI ApoI AspHI AvaII
BalI BanI BanII BbsI BbvI
BfaI BfrI BlpI BmyI BpmI
Bpu1102I BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstXI BstYI
Cac8I CelII Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HincII HindII HinfI HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MvaI MwoI
NcoI NdeII NheI NlaIII NlaIV
NspI PleI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI Tru9I TseI
Tsp45I Tsp509I TspRI XcmI XhoII