################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## Acc65I AccI AclI AgeI AhdI Aor51HI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII BbeI BglI BsaBI BsiWI BspDI BsrBI Bst1107I BstEII BstPI BstZ17I ClaI CpoI DrdI Eam1105I Eco47III EcoO65I EcoRI EcoT22I FseI FspI HaeII HpaI KasI KpnI KspI MamI MluI NarI NdeI NheI NotI NruI NsiI PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI Sse8387I SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 37 enzymes) # ################################### AatII 1214 GACGTC GACGT^C Alw44I 747 GTGCAC G^TGCAC AlwNI 1554 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG ApaLI 747 GTGCAC G^TGCAC BanI 604 GGCACC G^G[CT][GA]CC BcgI 93 CGATCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BglII 1639 AGATCT A^GATCT BsmBI 3987 CGTCTC CGTCTC(1/5) Bsp1407I 3066 TGTACA T^GTACA BsrFI 764 GCCGGC [GA]^CCGG[CT] BsrGI 3066 TGTACA T^GTACA BssHII 181 GCGCGC G^CGCGC BssSI 4282 CACGAG CACGAG(-5/-1) BstBI 1534 TTCGAA TT^CGAA Cfr10I 764 GCCGGC [GA]^CCGG[CT] DraIII 3332 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 242 CGGCCG C^GGCCG EclXI 242 CGGCCG C^GGCCG Eco52I 242 CGGCCG C^GGCCG EcoNI 2920 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 15 CCCGC CCCGC(4/6) HincII 3550 GTCAAC GT[CT]^[GA]AC HindII 3550 GTCAAC GT[CT]^[GA]AC MfeI 2598 CAATTG C^AATTG MunI 2598 CAATTG C^AATTG NaeI 764 GCCGGC GCC^GGC NcoI 1405 CCATGG C^CATGG NgoMIV 764 GCCGGC G^CCGGC NspV 1534 TTCGAA TT^CGAA PaeR7I 580 CTCGAG C^TCGAG PshAI 1936 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PvuII 1938 CAGCTG CAG^CTG SfuI 1534 TTCGAA TT^CGAA SphI 3324 GCATGC GCATG^C SspBI 3066 TGTACA T^GTACA XcmI 421 CCAACGAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 580 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AcyI 117 GGCGTC G[GA]^CG[CT]C 1214 GACGTC AflII 3013 CTTAAG C^TTAAG 3690 CTTAAG AflIII 1626 ACATGT A^C[GA][CT]GT 4112 ACATGT AocI 710 CCTGAGG CC^T[ACGT]AGG 2266 CCTTAGG AvaI 31 CTCGGG C^[CT]CG[GA]G 580 CTCGAG AvrII 3421 CCTAGG C^CTAGG 3665 CCTAGG BalI 4 TGGCCA TGG^CCA 2797 TGGCCA BbvCI 2886 CCTCAGC CCTCAGC(-5/-2) 4765 GCTGAGG BciVI 3205 GGATAC GTATCC(6/5) 4054 GGATAC BclI 344 TGATCA T^GATCA 2932 TGATCA BfrI 3013 CTTAAG C^TTAAG 3690 CTTAAG BlnI 3421 CCTAGG C^CTAGG 3665 CCTAGG BlpI 386 GCTGAGC GC^T[ACGT]AGC 1857 GCTCAGC Bpu1102I 386 GCTGAGC GC^T[ACGT]AGC 1857 GCTCAGC BsaHI 117 GGCGTC G[GA]^CG[CT]C 1214 GACGTC BsaI 519 GAGACC GGTCTC(1/5) 567 GGTCTC BsiEI 242 CGGCCG CG[GA][CT]^CG 2109 CGACCG BsmI 3778 GCATTC GAATGC(1/-1) 4171 GCATTC BsoBI 31 CTCGGG C^[CT]CG[GA]G 580 CTCGAG BspLU11I 1626 ACATGT A^CATGT 4112 ACATGT BstAPI 533 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1520 GCAAATCCTGC Bsu36I 710 CCTGAGG CC^T[ACGT]AGG 2266 CCTTAGG CelII 386 GCTGAGC GC^T[ACGT]AGC 1857 GCTCAGC DraII 738 AGGACCC [GA]G^G[ACGT]CC[CT] 1729 GGGTCCC DsaI 1142 CCACGG C^C[GA][CT]GG 1405 CCATGG Eco81I 710 CCTGAGG CC^T[ACGT]AGG 2266 CCTTAGG EcoO109I 738 AGGACCC [GA]G^G[ACGT]CC[CT] 1729 GGGTCCC EcoRV 309 GATATC GAT^ATC 1704 GATATC FbaI 344 TGATCA T^GATCA 2932 TGATCA Hin1I 117 GGCGTC G[GA]^CG[CT]C 1214 GACGTC MluNI 4 TGGCCA TGG^CCA 2797 TGGCCA MscI 4 TGGCCA TGG^CCA 2797 TGGCCA MspA1I 1290 CAGCGG C[AC]G^C[GT]G 1938 CAGCTG PflMI 1553 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4755 CCAGGTGGTGG PpuMI 738 AGGACCC [GA]G^G[AT]CC[CT] 1729 GGGTCCC StuI 2659 AGGCCT AGG^CCT 3632 AGGCCT Van91I 1553 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 4755 CCAGGTGGTGG ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccII AccIII AciI AcsI AfaI AluI AlwI ApoI Asp700I AspHI AvaII BamHI BanII BbrPI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BsaWI BseAI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspEI BspHI BspMI BsrDI BsrI BssKI BstF5I BstNI BstUI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MroI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PstI RcaI RsaI SacI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoII XmnI