##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
Acc65I AccI AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII BbeI
BglI BsaBI BsiWI BspDI BsrBI
Bst1107I BstEII BstPI BstZ17I ClaI
CpoI DrdI Eam1105I Eco47III EcoO65I
EcoRI EcoT22I FseI FspI HaeII
HpaI KasI KpnI KspI MamI
MluI NarI NdeI NheI NotI
NruI NsiI PacI PflFI PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SgrAI SmaI SnaBI
SpeI Sse8387I SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 37 enzymes) #
###################################
AatII 1214 GACGTC GACGT^C
Alw44I 747 GTGCAC G^TGCAC
AlwNI 1554 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 747 GTGCAC G^TGCAC
BanI 604 GGCACC G^G[CT][GA]CC
BcgI 93 CGATCTCGGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 1639 AGATCT A^GATCT
BsmBI 3987 CGTCTC CGTCTC(1/5)
Bsp1407I 3066 TGTACA T^GTACA
BsrFI 764 GCCGGC [GA]^CCGG[CT]
BsrGI 3066 TGTACA T^GTACA
BssHII 181 GCGCGC G^CGCGC
BssSI 4282 CACGAG CACGAG(-5/-1)
BstBI 1534 TTCGAA TT^CGAA
Cfr10I 764 GCCGGC [GA]^CCGG[CT]
DraIII 3332 CACTCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 242 CGGCCG C^GGCCG
EclXI 242 CGGCCG C^GGCCG
Eco52I 242 CGGCCG C^GGCCG
EcoNI 2920 CCTGTGTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 15 CCCGC CCCGC(4/6)
HincII 3550 GTCAAC GT[CT]^[GA]AC
HindII 3550 GTCAAC GT[CT]^[GA]AC
MfeI 2598 CAATTG C^AATTG
MunI 2598 CAATTG C^AATTG
NaeI 764 GCCGGC GCC^GGC
NcoI 1405 CCATGG C^CATGG
NgoMIV 764 GCCGGC G^CCGGC
NspV 1534 TTCGAA TT^CGAA
PaeR7I 580 CTCGAG C^TCGAG
PshAI 1936 GACAGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1938 CAGCTG CAG^CTG
SfuI 1534 TTCGAA TT^CGAA
SphI 3324 GCATGC GCATG^C
SspBI 3066 TGTACA T^GTACA
XcmI 421 CCAACGAATCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 580 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AcyI 117 GGCGTC G[GA]^CG[CT]C
1214 GACGTC
AflII 3013 CTTAAG C^TTAAG
3690 CTTAAG
AflIII 1626 ACATGT A^C[GA][CT]GT
4112 ACATGT
AocI 710 CCTGAGG CC^T[ACGT]AGG
2266 CCTTAGG
AvaI 31 CTCGGG C^[CT]CG[GA]G
580 CTCGAG
AvrII 3421 CCTAGG C^CTAGG
3665 CCTAGG
BalI 4 TGGCCA TGG^CCA
2797 TGGCCA
BbvCI 2886 CCTCAGC CCTCAGC(-5/-2)
4765 GCTGAGG
BciVI 3205 GGATAC GTATCC(6/5)
4054 GGATAC
BclI 344 TGATCA T^GATCA
2932 TGATCA
BfrI 3013 CTTAAG C^TTAAG
3690 CTTAAG
BlnI 3421 CCTAGG C^CTAGG
3665 CCTAGG
BlpI 386 GCTGAGC GC^T[ACGT]AGC
1857 GCTCAGC
Bpu1102I 386 GCTGAGC GC^T[ACGT]AGC
1857 GCTCAGC
BsaHI 117 GGCGTC G[GA]^CG[CT]C
1214 GACGTC
BsaI 519 GAGACC GGTCTC(1/5)
567 GGTCTC
BsiEI 242 CGGCCG CG[GA][CT]^CG
2109 CGACCG
BsmI 3778 GCATTC GAATGC(1/-1)
4171 GCATTC
BsoBI 31 CTCGGG C^[CT]CG[GA]G
580 CTCGAG
BspLU11I 1626 ACATGT A^CATGT
4112 ACATGT
BstAPI 533 GCACTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1520 GCAAATCCTGC
Bsu36I 710 CCTGAGG CC^T[ACGT]AGG
2266 CCTTAGG
CelII 386 GCTGAGC GC^T[ACGT]AGC
1857 GCTCAGC
DraII 738 AGGACCC [GA]G^G[ACGT]CC[CT]
1729 GGGTCCC
DsaI 1142 CCACGG C^C[GA][CT]GG
1405 CCATGG
Eco81I 710 CCTGAGG CC^T[ACGT]AGG
2266 CCTTAGG
EcoO109I 738 AGGACCC [GA]G^G[ACGT]CC[CT]
1729 GGGTCCC
EcoRV 309 GATATC GAT^ATC
1704 GATATC
FbaI 344 TGATCA T^GATCA
2932 TGATCA
Hin1I 117 GGCGTC G[GA]^CG[CT]C
1214 GACGTC
MluNI 4 TGGCCA TGG^CCA
2797 TGGCCA
MscI 4 TGGCCA TGG^CCA
2797 TGGCCA
MspA1I 1290 CAGCGG C[AC]G^C[GT]G
1938 CAGCTG
PflMI 1553 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4755 CCAGGTGGTGG
PpuMI 738 AGGACCC [GA]G^G[AT]CC[CT]
1729 GGGTCCC
StuI 2659 AGGCCT AGG^CCT
3632 AGGCCT
Van91I 1553 CCAGAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4755 CCAGGTGGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccII AccIII AciI AcsI AfaI
AluI AlwI ApoI Asp700I AspHI
AvaII BamHI BanII BbrPI BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaAI BsaJI BsaWI BseAI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspEI BspHI
BspMI BsrDI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
EaeI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HindIII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MroI
MseI MslI MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
RcaI RsaI SacI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XbaI XhoII XmnI