################################## # NO CUT ENZYMES ( 89 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AcyI AflII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I Asp718I AspI AviII AvrII BbeI BcgI BciVI BfrI BglII BlnI BsaHI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrGI Bst1107I BstBI BstZ17I ClaI CpoI DraI EagI EclXI Eco52I EcoRI EcoRV FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MfeI MluI MroI MseI MunI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacI SacII SalI SfiI SfoI SfuI SgrAI SnaBI SpeI SspBI SwaI Tru9I Tth111I XmnI ################################### # ONE CUT ENZYMES ( 29 enzymes) # ################################### AcsI 3534 AAATTT [GA]^AATT[CT] Aor51HI 829 AGCGCT AGC^GCT ApoI 3534 AAATTT [GA]^AATT[CT] BclI 1623 TGATCA T^GATCA BsaBI 1947 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaI 1138 GAGACC GGTCTC(1/5) BsmBI 3497 GAGACG CGTCTC(1/5) BsrFI 2555 GCCGGC [GA]^CCGG[CT] BssHII 2460 GCGCGC G^CGCGC BssSI 4034 CACGAG CACGAG(-5/-1) BstAPI 3227 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC Cfr10I 2555 GCCGGC [GA]^CCGG[CT] DrdI 909 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 829 AGCGCT AGC^GCT EcoT22I 1294 ATGCAT ATGCA^T FbaI 1623 TGATCA T^GATCA MamI 1947 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 2555 GCCGGC GCC^GGC NgoMIV 2555 GCCGGC G^CCGGC NheI 756 GCTAGC G^CTAGC NsiI 1294 ATGCAT ATGCA^T PaeR7I 586 CTCGAG C^TCGAG SapI 262 GAAGAGC GCTCTTC(1/4) SbfI 3098 CCTGCAGG CCTGCA^GG SexAI 1404 ACCAGGT A^CC[AT]GGT Sse8387I 3098 CCTGCAGG CCTGCA^GG SspI 3701 AATATT AAT^ATT StuI 1050 AGGCCT AGG^CCT XhoI 586 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AhdI 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4047 GACACCTTGTC AspEI 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4047 GACACCTTGTC BamHI 1451 GGATCC G^GATCC 4094 GGATCC BbrPI 2141 CACGTG CAC^GTG 2986 CACGTG BglI 364 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1543 GCCATCAAGGC BlpI 75 GCTCAGC GC^T[ACGT]AGC 2532 GCTGAGC Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC 2532 GCTGAGC CelII 75 GCTCAGC GC^T[ACGT]AGC 2532 GCTGAGC DraIII 3869 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG 4075 CACCCAGTG Eam1105I 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 4047 GACACCTTGTC HincII 793 GTCAAC GT[CT]^[GA]AC 841 GTCAAC HindII 793 GTCAAC GT[CT]^[GA]AC 841 GTCAAC PmaCI 2141 CACGTG CAC^GTG 2986 CACGTG PmlI 2141 CACGTG CAC^GTG 2986 CACGTG PshAI 1173 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2671 GACAGAGGTC ScaI 977 AGTACT AGT^ACT 3977 AGTACT XbaI 1710 TCTAGA T^CTAGA 2761 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 123 enzymes) # ######################################### AccII AciI AfaI AflIII AluI AlwI AlwNI AocI ApaI AspHI AvaI AvaII BalI BanI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrDI BsrI BssKI BstEII BstF5I BstNI BstPI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI Eco81I EcoNI EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmaI SmlI SphI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI XhoII XmaCI XmaI