##################################
# NO CUT ENZYMES ( 89 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcyI AflII AgeI Alw44I ApaLI
AscI AseI AsnI Asp700I Asp718I
AspI AviII AvrII BbeI BcgI
BciVI BfrI BglII BlnI BsaHI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrGI Bst1107I BstBI BstZ17I ClaI
CpoI DraI EagI EclXI Eco52I
EcoRI EcoRV FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MfeI MluI MroI MseI
MunI NarI NdeI NotI NruI
NspV PacI PflFI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SfiI SfoI
SfuI SgrAI SnaBI SpeI SspBI
SwaI Tru9I Tth111I XmnI
###################################
# ONE CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 3534 AAATTT [GA]^AATT[CT]
Aor51HI 829 AGCGCT AGC^GCT
ApoI 3534 AAATTT [GA]^AATT[CT]
BclI 1623 TGATCA T^GATCA
BsaBI 1947 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1138 GAGACC GGTCTC(1/5)
BsmBI 3497 GAGACG CGTCTC(1/5)
BsrFI 2555 GCCGGC [GA]^CCGG[CT]
BssHII 2460 GCGCGC G^CGCGC
BssSI 4034 CACGAG CACGAG(-5/-1)
BstAPI 3227 GCAGCCTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cfr10I 2555 GCCGGC [GA]^CCGG[CT]
DrdI 909 GACCAGCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 829 AGCGCT AGC^GCT
EcoT22I 1294 ATGCAT ATGCA^T
FbaI 1623 TGATCA T^GATCA
MamI 1947 GATCCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 2555 GCCGGC GCC^GGC
NgoMIV 2555 GCCGGC G^CCGGC
NheI 756 GCTAGC G^CTAGC
NsiI 1294 ATGCAT ATGCA^T
PaeR7I 586 CTCGAG C^TCGAG
SapI 262 GAAGAGC GCTCTTC(1/4)
SbfI 3098 CCTGCAGG CCTGCA^GG
SexAI 1404 ACCAGGT A^CC[AT]GGT
Sse8387I 3098 CCTGCAGG CCTGCA^GG
SspI 3701 AATATT AAT^ATT
StuI 1050 AGGCCT AGG^CCT
XhoI 586 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AhdI 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4047 GACACCTTGTC
AspEI 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4047 GACACCTTGTC
BamHI 1451 GGATCC G^GATCC
4094 GGATCC
BbrPI 2141 CACGTG CAC^GTG
2986 CACGTG
BglI 364 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1543 GCCATCAAGGC
BlpI 75 GCTCAGC GC^T[ACGT]AGC
2532 GCTGAGC
Bpu1102I 75 GCTCAGC GC^T[ACGT]AGC
2532 GCTGAGC
CelII 75 GCTCAGC GC^T[ACGT]AGC
2532 GCTGAGC
DraIII 3869 CACCCCGTG CAC[ACGT][ACGT][ACGT]^GTG
4075 CACCCAGTG
Eam1105I 2749 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4047 GACACCTTGTC
HincII 793 GTCAAC GT[CT]^[GA]AC
841 GTCAAC
HindII 793 GTCAAC GT[CT]^[GA]AC
841 GTCAAC
PmaCI 2141 CACGTG CAC^GTG
2986 CACGTG
PmlI 2141 CACGTG CAC^GTG
2986 CACGTG
PshAI 1173 GACCATCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2671 GACAGAGGTC
ScaI 977 AGTACT AGT^ACT
3977 AGTACT
XbaI 1710 TCTAGA T^CTAGA
2761 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwI AlwNI AocI ApaI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrDI BsrI BssKI BstEII
BstF5I BstNI BstPI BstUI BstXI
BstYI Bsu36I Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XhoII XmaCI XmaI