##################################
# NO CUT ENZYMES ( 59 enzymes) #
##################################
AclI AhdI Alw44I AocI ApaLI
AscI AseI AsnI AspEI AviII
BamHI BbrPI BglII BsaAI BsiWI
Bst1107I BstBI BstYI BstZ17I Bsu36I
CpoI DrdI Eam1105I Eco81I EcoRV
EcoT22I FspI MfeI MflI MluI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PmaCI PmeI PmlI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I SwaI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
Acc65I 2371 GGTACC G^GTACC
AccIII 1096 TCCGGA T^CCGGA
AflII 3591 CTTAAG C^TTAAG
AgeI 1840 ACCGGT A^CCGGT
Aor51HI 3211 AGCGCT AGC^GCT
ApaI 1326 GGGCCC GGGCC^C
Asp718I 2371 GGTACC G^GTACC
AspI 1112 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 210 CCTAGG C^CTAGG
BbvCI 1321 GCTGAGG CCTCAGC(-5/-2)
BcgI 4094 CGACTCTCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1665 TGATCA T^GATCA
BfrI 3591 CTTAAG C^TTAAG
BlnI 210 CCTAGG C^CTAGG
BlpI 2727 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2727 GCTCAGC GC^T[ACGT]AGC
BsaBI 2557 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 1096 TCCGGA T^CCGGA
BsmBI 5 CGTCTC CGTCTC(1/5)
Bsp1407I 4329 TGTACA T^GTACA
BspDI 2930 ATCGAT AT^CGAT
BspEI 1096 TCCGGA T^CCGGA
BspHI 3521 TCATGA T^CATGA
BsrDI 262 CATTGC GCAATG(2/0)
BsrGI 4329 TGTACA T^GTACA
BssHII 135 GCGCGC G^CGCGC
BssSI 3871 CACGAG CACGAG(-5/-1)
BstEII 4049 GGTGACC G^GT[ACGT]ACC
BstPI 4049 GGTGACC G^GT[ACGT]ACC
BstXI 3255 CCACCTCCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2727 GCTCAGC GC^T[ACGT]AGC
ClaI 2930 ATCGAT AT^CGAT
DraI 3776 TTTAAA TTT^AAA
Eco47III 3211 AGCGCT AGC^GCT
EcoO65I 4049 GGTGACC G^GT[ACGT]ACC
EcoRI 1154 GAATTC G^AATTC
FbaI 1665 TGATCA T^GATCA
FseI 4074 GGCCGGCC GGCCGG^CC
HindIII 4362 AAGCTT A^AGCTT
HpaI 3621 GTTAAC GTT^AAC
KpnI 2371 GGTACC GGTAC^C
MamI 2557 GATGGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 1096 TCCGGA T^CCGGA
NaeI 4075 GCCGGC GCC^GGC
NgoMIV 4075 GCCGGC G^CCGGC
PflFI 1112 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 1840 ACCGGT A^CCGGT
PshAI 2797 GACACTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3521 TCATGA T^CATGA
SapI 3272 GAAGAGC GCTCTTC(1/4)
ScaI 766 AGTACT AGT^ACT
SfiI 408 GGCCTCGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 4329 TGTACA T^GTACA
SspI 3516 AATATT AAT^ATT
Tth111I 1112 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3541 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflIII 189 ACATGT A^C[GA][CT]GT
275 ACATGT
Asp700I 996 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3533 GAACATTTTC
AspHI 941 GAGCTC G[AT]GC[AT]^C
1764 GAGCTC
BciVI 2857 GGATAC GTATCC(6/5)
1139 GTATCC
BsaWI 1096 TCCGGA [AT]^CCGG[AT]
1840 ACCGGT
BsiEI 4073 CGGCCG CG[GA][CT]^CG
4077 CGGCCG
BsiHKAI 941 GAGCTC G[AT]GC[AT]^C
1764 GAGCTC
BspLU11I 189 ACATGT A^CATGT
275 ACATGT
BstAPI 632 GCAGTCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
641 GCAGTGAATGC
EagI 4073 CGGCCG C^GGCCG
4077 CGGCCG
EclXI 4073 CGGCCG C^GGCCG
4077 CGGCCG
Eco52I 4073 CGGCCG C^GGCCG
4077 CGGCCG
HgaI 132 GACGC GACGC(5/10)
4 GCGTC
HincII 3621 GTTAAC GT[CT]^[GA]AC
4122 GTCAAC
HindII 3621 GTTAAC GT[CT]^[GA]AC
4122 GTCAAC
KspI 122 CCGCGG CCGC^GG
486 CCGCGG
PvuII 1373 CAGCTG CAG^CTG
2336 CAGCTG
SacI 941 GAGCTC GAGCT^C
1764 GAGCTC
SacII 122 CCGCGG CCGC^GG
486 CCGCGG
XmnI 996 GAAACTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3533 GAACATTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 123 enzymes) #
#########################################
AatII AccI AccII AciI AcsI
AcyI AfaI AluI AlwI AlwNI
ApoI AvaI AvaII BalI BanI
BanII BbeI BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspMI BsrBI BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmaI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XmaCI XmaI