################################## # NO CUT ENZYMES ( 74 enzymes) # ################################## AccIII AclI AgeI AhdI AscI AseI AsnI AspEI AvrII BalI BamHI BbvCI BcgI BglI BlnI BsaWI BseAI BsiWI Bsp1407I BspEI BspLU11I BsrDI BsrFI BsrGI Bst1107I BstEII BstPI BstXI BstZ17I Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco52I EcoO65I EcoRI EcoRV FseI KspI MluI MluNI MroI MscI NaeI NgoMIV NheI NotI NruI PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RsrII SacII SapI SbfI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### Alw44I 2116 GTGCAC G^TGCAC ApaI 2073 GGGCCC GGGCC^C ApaLI 2116 GTGCAC G^TGCAC AspI 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 5 TGCGCA TGC^GCA BbeI 3904 GGCGCC GGCGC^C BbrPI 3214 CACGTG CAC^GTG BclI 1601 TGATCA T^GATCA BlpI 3796 GCTCAGC GC^T[ACGT]AGC Bpu1102I 3796 GCTCAGC GC^T[ACGT]AGC BsaAI 3214 CACGTG [CT]AC^GT[GA] BsmBI 1551 CGTCTC CGTCTC(1/5) BspDI 1573 ATCGAT AT^CGAT BspHI 3233 TCATGA T^CATGA BspMI 2172 ACCTGC ACCTGC(4/8) BssHII 201 GCGCGC G^CGCGC CelII 3796 GCTCAGC GC^T[ACGT]AGC ClaI 1573 ATCGAT AT^CGAT FbaI 1601 TGATCA T^GATCA FspI 5 TGCGCA TGC^GCA HindIII 2443 AAGCTT A^AGCTT HpaI 791 GTTAAC GTT^AAC KasI 3904 GGCGCC G^GCGCC MfeI 845 CAATTG C^AATTG MunI 845 CAATTG C^AATTG NarI 3904 GGCGCC GG^CGCC PflFI 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 3214 CACGTG CAC^GTG PmlI 3214 CACGTG CAC^GTG PvuI 31 CGATCG CGAT^CG RcaI 3233 TCATGA T^CATGA SalI 1303 GTCGAC G^TCGAC SexAI 1324 ACCAGGT A^CC[AT]GGT SfoI 3904 GGCGCC GGC^GCC Tth111I 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 37 enzymes) # ################################### AccI 1303 GTCGAC GT^[AC][GT]AC 4120 GTAGAC AcsI 1744 AAATTT [GA]^AATT[CT] 1753 AAATTT AflIII 3215 ACGTGT A^C[GA][CT]GT 3356 ACGTGT AocI 3022 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG Aor51HI 3167 AGCGCT AGC^GCT 3307 AGCGCT ApoI 1744 AAATTT [GA]^AATT[CT] 1753 AAATTT AvaI 2973 CTCGGG C^[CT]CG[GA]G 4317 CTCGGG BciVI 1915 GGATAC GTATCC(6/5) 3450 GGATAC BglII 1536 AGATCT A^GATCT 3731 AGATCT BsaBI 220 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1602 GATCAGCATC BsaI 1124 GGTCTC GGTCTC(1/5) 2572 GGTCTC BsiEI 31 CGATCG CG[GA][CT]^CG 3933 CGACCG BsoBI 2973 CTCGGG C^[CT]CG[GA]G 4317 CTCGGG BsrBI 3936 CCGCTC CCGCTC(-3/-3) 1073 GAGCGG BssSI 1363 CTCGTG CACGAG(-5/-1) 2023 CTCGTG BstAPI 2290 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2415 GCAAGAGCTGC BstBI 409 TTCGAA TT^CGAA 2551 TTCGAA Bsu36I 3022 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG EaeI 117 CGGCCA [CT]^GGCC[GA] 3603 CGGCCA EarI 1805 CTCTTC CTCTTC(1/4) 1417 GAAGAG Eco47III 3167 AGCGCT AGC^GCT 3307 AGCGCT Eco81I 3022 CCTGAGG CC^T[ACGT]AGG 3565 CCTCAGG EcoNI 1085 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 3621 CCTCCACCAGG EcoT22I 214 ATGCAT ATGCA^T 1883 ATGCAT Ksp632I 1805 CTCTTC CTCTTC(1/4) 1417 GAAGAG MamI 220 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1602 GATCAGCATC NdeI 2324 CATATG CA^TATG 2334 CATATG NsiI 214 ATGCAT ATGCA^T 1883 ATGCAT NspI 1842 ACATGC [GA]CATG^[CT] 3296 ACATGC NspV 409 TTCGAA TT^CGAA 2551 TTCGAA PflMI 2536 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3515 CCACTGGCTGG PpuMI 1721 AGGACCT [GA]G^G[AT]CC[CT] 2163 AGGACCT SacI 3794 GAGCTC GAGCT^C 4256 GAGCTC SfuI 409 TTCGAA TT^CGAA 2551 TTCGAA SspI 588 AATATT AAT^ATT 4627 AATATT StuI 3238 AGGCCT AGG^CCT 3744 AGGCCT Van91I 2536 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3515 CCACTGGCTGG ######################################### # 3 OR MORE CUT ENZYMES ( 112 enzymes) # ######################################### AatII Acc65I AccII AciI AcyI AfaI AflII AluI AlwI AlwNI Asp700I Asp718I AspHI AvaII BanI BanII BbsI BbvI BcnI BfaI BfrI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI DsaI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KpnI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScaI ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII XmnI