##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AccIII AclI AgeI AhdI AscI
AseI AsnI AspEI AvrII BalI
BamHI BbvCI BcgI BglI BlnI
BsaWI BseAI BsiWI Bsp1407I BspEI
BspLU11I BsrDI BsrFI BsrGI Bst1107I
BstEII BstPI BstXI BstZ17I Cfr10I
CpoI DraIII EagI Eam1105I EclXI
Eco52I EcoO65I EcoRI EcoRV FseI
KspI MluI MluNI MroI MscI
NaeI NgoMIV NheI NotI NruI
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I RsrII SacII SapI
SbfI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
Alw44I 2116 GTGCAC G^TGCAC
ApaI 2073 GGGCCC GGGCC^C
ApaLI 2116 GTGCAC G^TGCAC
AspI 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 5 TGCGCA TGC^GCA
BbeI 3904 GGCGCC GGCGC^C
BbrPI 3214 CACGTG CAC^GTG
BclI 1601 TGATCA T^GATCA
BlpI 3796 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 3796 GCTCAGC GC^T[ACGT]AGC
BsaAI 3214 CACGTG [CT]AC^GT[GA]
BsmBI 1551 CGTCTC CGTCTC(1/5)
BspDI 1573 ATCGAT AT^CGAT
BspHI 3233 TCATGA T^CATGA
BspMI 2172 ACCTGC ACCTGC(4/8)
BssHII 201 GCGCGC G^CGCGC
CelII 3796 GCTCAGC GC^T[ACGT]AGC
ClaI 1573 ATCGAT AT^CGAT
FbaI 1601 TGATCA T^GATCA
FspI 5 TGCGCA TGC^GCA
HindIII 2443 AAGCTT A^AGCTT
HpaI 791 GTTAAC GTT^AAC
KasI 3904 GGCGCC G^GCGCC
MfeI 845 CAATTG C^AATTG
MunI 845 CAATTG C^AATTG
NarI 3904 GGCGCC GG^CGCC
PflFI 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 3214 CACGTG CAC^GTG
PmlI 3214 CACGTG CAC^GTG
PvuI 31 CGATCG CGAT^CG
RcaI 3233 TCATGA T^CATGA
SalI 1303 GTCGAC G^TCGAC
SexAI 1324 ACCAGGT A^CC[AT]GGT
SfoI 3904 GGCGCC GGC^GCC
Tth111I 4146 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
AccI 1303 GTCGAC GT^[AC][GT]AC
4120 GTAGAC
AcsI 1744 AAATTT [GA]^AATT[CT]
1753 AAATTT
AflIII 3215 ACGTGT A^C[GA][CT]GT
3356 ACGTGT
AocI 3022 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
Aor51HI 3167 AGCGCT AGC^GCT
3307 AGCGCT
ApoI 1744 AAATTT [GA]^AATT[CT]
1753 AAATTT
AvaI 2973 CTCGGG C^[CT]CG[GA]G
4317 CTCGGG
BciVI 1915 GGATAC GTATCC(6/5)
3450 GGATAC
BglII 1536 AGATCT A^GATCT
3731 AGATCT
BsaBI 220 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1602 GATCAGCATC
BsaI 1124 GGTCTC GGTCTC(1/5)
2572 GGTCTC
BsiEI 31 CGATCG CG[GA][CT]^CG
3933 CGACCG
BsoBI 2973 CTCGGG C^[CT]CG[GA]G
4317 CTCGGG
BsrBI 3936 CCGCTC CCGCTC(-3/-3)
1073 GAGCGG
BssSI 1363 CTCGTG CACGAG(-5/-1)
2023 CTCGTG
BstAPI 2290 GCAGTAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2415 GCAAGAGCTGC
BstBI 409 TTCGAA TT^CGAA
2551 TTCGAA
Bsu36I 3022 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
EaeI 117 CGGCCA [CT]^GGCC[GA]
3603 CGGCCA
EarI 1805 CTCTTC CTCTTC(1/4)
1417 GAAGAG
Eco47III 3167 AGCGCT AGC^GCT
3307 AGCGCT
Eco81I 3022 CCTGAGG CC^T[ACGT]AGG
3565 CCTCAGG
EcoNI 1085 CCTCCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
3621 CCTCCACCAGG
EcoT22I 214 ATGCAT ATGCA^T
1883 ATGCAT
Ksp632I 1805 CTCTTC CTCTTC(1/4)
1417 GAAGAG
MamI 220 GATTGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1602 GATCAGCATC
NdeI 2324 CATATG CA^TATG
2334 CATATG
NsiI 214 ATGCAT ATGCA^T
1883 ATGCAT
NspI 1842 ACATGC [GA]CATG^[CT]
3296 ACATGC
NspV 409 TTCGAA TT^CGAA
2551 TTCGAA
PflMI 2536 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3515 CCACTGGCTGG
PpuMI 1721 AGGACCT [GA]G^G[AT]CC[CT]
2163 AGGACCT
SacI 3794 GAGCTC GAGCT^C
4256 GAGCTC
SfuI 409 TTCGAA TT^CGAA
2551 TTCGAA
SspI 588 AATATT AAT^ATT
4627 AATATT
StuI 3238 AGGCCT AGG^CCT
3744 AGGCCT
Van91I 2536 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3515 CCACTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AatII Acc65I AccII AciI AcyI
AfaI AflII AluI AlwI AlwNI
Asp700I Asp718I AspHI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BfrI BmyI BpmI BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraI DraII DrdI DsaI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KpnI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NcoI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII XmnI