##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AccIII AclI AflIII AhdI
Alw44I ApaLI AscI AspEI AspI
AviII AvrII BbeI BbrPI BglI
BlnI BlpI Bpu1102I BsaBI BseAI
BsiWI BsmBI Bsp1407I BspDI BspEI
BspLU11I BsrBI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DrdI Eam1105I EcoT22I FspI KasI
MamI MfeI MluI MroI MunI
NarI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI Psp1406I RsrII SacI
SalI SapI SbfI ScaI SfiI
SfoI SfuI SgrAI Sse8387I SspBI
StuI SwaI TaqI Tth111I TthHB8I
XhoI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 1981 GGTACC G^GTACC
AccII 90 CGCG CG^CG
AcyI 339 GACGCC G[GA]^CG[CT]C
AflII 3580 CTTAAG C^TTAAG
AgeI 96 ACCGGT A^CCGGT
Aor51HI 151 AGCGCT AGC^GCT
ApaI 1241 GGGCCC GGGCC^C
AseI 2630 ATTAAT AT^TAAT
AsnI 2630 ATTAAT AT^TAAT
Asp700I 3535 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1981 GGTACC G^GTACC
BalI 770 TGGCCA TGG^CCA
BamHI 25 GGATCC G^GATCC
BbvCI 2534 GCTGAGG CCTCAGC(-5/-2)
BcgI 1279 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 3580 CTTAAG C^TTAAG
BglII 1354 AGATCT A^GATCT
BsaAI 579 TACGTA [CT]AC^GT[GA]
BsaHI 339 GACGCC G[GA]^CG[CT]C
BsaWI 96 ACCGGT [AT]^CCGG[AT]
BsmI 206 GAATGC GAATGC(1/-1)
BspHI 790 TCATGA T^CATGA
BsrDI 3385 GCAATG GCAATG(2/0)
BssSI 1334 CTCGTG CACGAG(-5/-1)
BstAPI 1635 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2011 GGTTACC G^GT[ACGT]ACC
BstPI 2011 GGTTACC G^GT[ACGT]ACC
BstUI 90 CGCG CG^CG
DraIII 1402 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 49 CGGCCG C^GGCCG
EarI 2278 GAAGAG CTCTTC(1/4)
EclXI 49 CGGCCG C^GGCCG
Eco47III 151 AGCGCT AGC^GCT
Eco52I 49 CGGCCG C^GGCCG
EcoNI 1826 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2011 GGTTACC G^GT[ACGT]ACC
FseI 46 GGCCGGCC GGCCGG^CC
HgaI 339 GACGC GACGC(5/10)
Hin1I 339 GACGCC G[GA]^CG[CT]C
HindIII 1298 AAGCTT A^AGCTT
HpaI 3422 GTTAAC GTT^AAC
KpnI 1981 GGTACC GGTAC^C
Ksp632I 2278 GAAGAG CTCTTC(1/4)
KspI 89 CCGCGG CCGC^GG
MluNI 770 TGGCCA TGG^CCA
MscI 770 TGGCCA TGG^CCA
MvnI 90 CGCG CG^CG
NdeI 2923 CATATG CA^TATG
PinAI 96 ACCGGT A^CCGGT
PshAI 2483 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 2630 ATTAAT AT^TAAT
PvuI 643 CGATCG CGAT^CG
RcaI 790 TCATGA T^CATGA
SacII 89 CCGCGG CCGC^GG
SexAI 2716 ACCAGGT A^CC[AT]GGT
SnaBI 579 TACGTA TAC^GTA
SpeI 3537 ACTAGT A^CTAGT
SspI 3108 AATATT AAT^ATT
XbaI 2491 TCTAGA T^CTAGA
XcmI 2324 CCATCCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 3535 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2558 GTAGAC GT^[AC][GT]AC
3283 GTCTAC
AocI 29 CCTCAGG CC^T[ACGT]AGG
555 CCTGAGG
AspHI 1397 GAGCAC G[AT]GC[AT]^C
1850 GTGCTC
AvaI 21 CCCGGG C^[CT]CG[GA]G
70 CCCGGG
BciVI 2504 GGATAC GTATCC(6/5)
582 GTATCC
BclI 2311 TGATCA T^GATCA
2736 TGATCA
BsiEI 49 CGGCCG CG[GA][CT]^CG
643 CGATCG
BsiHKAI 1397 GAGCAC G[AT]GC[AT]^C
1850 GTGCTC
BsoBI 21 CCCGGG C^[CT]CG[GA]G
70 CCCGGG
BstXI 185 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2016 CCAGTGATATGG
Bsu36I 29 CCTCAGG CC^T[ACGT]AGG
555 CCTGAGG
CfoI 152 GCGC GCG^C
456 GCGC
DraI 923 TTTAAA TTT^AAA
2423 TTTAAA
Eco81I 29 CCTCAGG CC^T[ACGT]AGG
555 CCTGAGG
EcoRI 573 GAATTC G^AATTC
2880 GAATTC
EcoRV 1216 GATATC GAT^ATC
2439 GATATC
FbaI 2311 TGATCA T^GATCA
2736 TGATCA
HaeII 151 AGCGCT [GA]GCGC^[CT]
455 GGCGCT
HhaI 152 GCGC GCG^C
456 GCGC
HinP1I 152 GCGC G^CGC
456 GCGC
MslI 804 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2303 CATGGCAGTG
NaeI 47 GCCGGC GCC^GGC
51 GCCGGC
NgoMIV 47 GCCGGC G^CCGGC
51 GCCGGC
SmaI 21 CCCGGG CCC^GGG
70 CCCGGG
XmaCI 21 CCCGGG C^CCGGG
70 CCCGGG
XmaI 21 CCCGGG C^CCGGG
70 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 95 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrFI BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SphI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Van91I XhoII