################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AatII AccIII AclI AflIII AhdI Alw44I ApaLI AscI AspEI AspI AviII AvrII BbeI BbrPI BglI BlnI BlpI Bpu1102I BsaBI BseAI BsiWI BsmBI Bsp1407I BspDI BspEI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstZ17I CelII ClaI CpoI DrdI Eam1105I EcoT22I FspI KasI MamI MfeI MluI MroI MunI NarI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PmaCI PmeI PmlI Psp1406I RsrII SacI SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI Sse8387I SspBI StuI SwaI TaqI Tth111I TthHB8I XhoI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 1981 GGTACC G^GTACC AccII 90 CGCG CG^CG AcyI 339 GACGCC G[GA]^CG[CT]C AflII 3580 CTTAAG C^TTAAG AgeI 96 ACCGGT A^CCGGT Aor51HI 151 AGCGCT AGC^GCT ApaI 1241 GGGCCC GGGCC^C AseI 2630 ATTAAT AT^TAAT AsnI 2630 ATTAAT AT^TAAT Asp700I 3535 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1981 GGTACC G^GTACC BalI 770 TGGCCA TGG^CCA BamHI 25 GGATCC G^GATCC BbvCI 2534 GCTGAGG CCTCAGC(-5/-2) BcgI 1279 GCACGGTGTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BfrI 3580 CTTAAG C^TTAAG BglII 1354 AGATCT A^GATCT BsaAI 579 TACGTA [CT]AC^GT[GA] BsaHI 339 GACGCC G[GA]^CG[CT]C BsaWI 96 ACCGGT [AT]^CCGG[AT] BsmI 206 GAATGC GAATGC(1/-1) BspHI 790 TCATGA T^CATGA BsrDI 3385 GCAATG GCAATG(2/0) BssSI 1334 CTCGTG CACGAG(-5/-1) BstAPI 1635 GCAGCACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 2011 GGTTACC G^GT[ACGT]ACC BstPI 2011 GGTTACC G^GT[ACGT]ACC BstUI 90 CGCG CG^CG DraIII 1402 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 49 CGGCCG C^GGCCG EarI 2278 GAAGAG CTCTTC(1/4) EclXI 49 CGGCCG C^GGCCG Eco47III 151 AGCGCT AGC^GCT Eco52I 49 CGGCCG C^GGCCG EcoNI 1826 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 2011 GGTTACC G^GT[ACGT]ACC FseI 46 GGCCGGCC GGCCGG^CC HgaI 339 GACGC GACGC(5/10) Hin1I 339 GACGCC G[GA]^CG[CT]C HindIII 1298 AAGCTT A^AGCTT HpaI 3422 GTTAAC GTT^AAC KpnI 1981 GGTACC GGTAC^C Ksp632I 2278 GAAGAG CTCTTC(1/4) KspI 89 CCGCGG CCGC^GG MluNI 770 TGGCCA TGG^CCA MscI 770 TGGCCA TGG^CCA MvnI 90 CGCG CG^CG NdeI 2923 CATATG CA^TATG PinAI 96 ACCGGT A^CCGGT PshAI 2483 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 2630 ATTAAT AT^TAAT PvuI 643 CGATCG CGAT^CG RcaI 790 TCATGA T^CATGA SacII 89 CCGCGG CCGC^GG SexAI 2716 ACCAGGT A^CC[AT]GGT SnaBI 579 TACGTA TAC^GTA SpeI 3537 ACTAGT A^CTAGT SspI 3108 AATATT AAT^ATT XbaI 2491 TCTAGA T^CTAGA XcmI 2324 CCATCCAGGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 3535 GAACTAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2558 GTAGAC GT^[AC][GT]AC 3283 GTCTAC AocI 29 CCTCAGG CC^T[ACGT]AGG 555 CCTGAGG AspHI 1397 GAGCAC G[AT]GC[AT]^C 1850 GTGCTC AvaI 21 CCCGGG C^[CT]CG[GA]G 70 CCCGGG BciVI 2504 GGATAC GTATCC(6/5) 582 GTATCC BclI 2311 TGATCA T^GATCA 2736 TGATCA BsiEI 49 CGGCCG CG[GA][CT]^CG 643 CGATCG BsiHKAI 1397 GAGCAC G[AT]GC[AT]^C 1850 GTGCTC BsoBI 21 CCCGGG C^[CT]CG[GA]G 70 CCCGGG BstXI 185 CCATGTTCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2016 CCAGTGATATGG Bsu36I 29 CCTCAGG CC^T[ACGT]AGG 555 CCTGAGG CfoI 152 GCGC GCG^C 456 GCGC DraI 923 TTTAAA TTT^AAA 2423 TTTAAA Eco81I 29 CCTCAGG CC^T[ACGT]AGG 555 CCTGAGG EcoRI 573 GAATTC G^AATTC 2880 GAATTC EcoRV 1216 GATATC GAT^ATC 2439 GATATC FbaI 2311 TGATCA T^GATCA 2736 TGATCA HaeII 151 AGCGCT [GA]GCGC^[CT] 455 GGCGCT HhaI 152 GCGC GCG^C 456 GCGC HinP1I 152 GCGC G^CGC 456 GCGC MslI 804 CACTGAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2303 CATGGCAGTG NaeI 47 GCCGGC GCC^GGC 51 GCCGGC NgoMIV 47 GCCGGC G^CCGGC 51 GCCGGC SmaI 21 CCCGGG CCC^GGG 70 CCCGGG XmaCI 21 CCCGGG C^CCGGG 70 CCCGGG XmaI 21 CCCGGG C^CCGGG 70 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 95 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AvaII BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SphI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII