##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AccIII AclI AhdI Aor51HI
AscI AseI AsnI Asp700I AspEI
AspI AviII BclI BseAI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BspHI BsrGI BssHII Bst1107I BstBI
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI MluI MroI
NaeI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SbfI ScaI SexAI
SfuI SgrAI SpeI Sse8387I SspBI
SspI Tth111I XbaI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
Acc65I 440 GGTACC G^GTACC
AccI 1469 GTCGAC GT^[AC][GT]AC
AcyI 3235 GGCGCC G[GA]^CG[CT]C
AgeI 2955 ACCGGT A^CCGGT
Alw44I 3116 GTGCAC G^TGCAC
ApaLI 3116 GTGCAC G^TGCAC
Asp718I 440 GGTACC G^GTACC
BbeI 3235 GGCGCC GGCGC^C
BbrPI 3296 CACGTG CAC^GTG
BbvCI 3261 GCTGAGG CCTCAGC(-5/-2)
BcgI 3498 CGAGCGGCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2121 GTATCC GTATCC(6/5)
BglII 2100 AGATCT A^GATCT
BsaBI 1906 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 3235 GGCGCC G[GA]^CG[CT]C
BsiEI 3358 CGGCCG CG[GA][CT]^CG
BsrFI 2955 ACCGGT [GA]^CCGG[CT]
Cfr10I 2955 ACCGGT [GA]^CCGG[CT]
EagI 3358 CGGCCG C^GGCCG
EclXI 3358 CGGCCG C^GGCCG
Eco52I 3358 CGGCCG C^GGCCG
HgaI 3267 GACGC GACGC(5/10)
Hin1I 3235 GGCGCC G[GA]^CG[CT]C
KasI 3235 GGCGCC G^GCGCC
KpnI 440 GGTACC GGTAC^C
KspI 3447 CCGCGG CCGC^GG
MamI 1906 GATGATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 3235 GGCGCC GG^CGCC
PinAI 2955 ACCGGT A^CCGGT
PmaCI 3296 CACGTG CAC^GTG
PmlI 3296 CACGTG CAC^GTG
SacII 3447 CCGCGG CCGC^GG
SalI 1469 GTCGAC G^TCGAC
SapI 3926 GCTCTTC GCTCTTC(1/4)
SfiI 1412 GGCCTCGGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 3235 GGCGCC GGC^GCC
SnaBI 3123 TACGTA TAC^GTA
SwaI 2933 ATTTAAAT ATTT^AAAT
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AflII 1701 CTTAAG C^TTAAG
2891 CTTAAG
AvrII 2227 CCTAGG C^CTAGG
2490 CCTAGG
BbsI 1315 GAAGAC GAAGAC(2/6)
3286 GTCTTC
BfrI 1701 CTTAAG C^TTAAG
2891 CTTAAG
BlnI 2227 CCTAGG C^CTAGG
2490 CCTAGG
BpuAI 1315 GAAGAC GAAGAC(2/6)
3286 GTCTTC
BsaWI 2955 ACCGGT [AT]^CCGG[AT]
3290 TCCGGT
BsrDI 151 CATTGC GCAATG(2/0)
558 CATTGC
BssSI 3482 CTCGTG CACGAG(-5/-1)
3562 CTCGTG
BstAPI 146 GCACTCATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3272 GCAGAGCATGC
BstEII 578 GGTGACC G^GT[ACGT]ACC
2375 GGTGACC
BstPI 578 GGTGACC G^GT[ACGT]ACC
2375 GGTGACC
EcoO65I 578 GGTGACC G^GT[ACGT]ACC
2375 GGTGACC
EcoRI 2708 GAATTC G^AATTC
2974 GAATTC
HincII 1469 GTCGAC GT[CT]^[GA]AC
3635 GTCAAC
HindII 1469 GTCGAC GT[CT]^[GA]AC
3635 GTCAAC
MfeI 1854 CAATTG C^AATTG
3040 CAATTG
MunI 1854 CAATTG C^AATTG
3040 CAATTG
NheI 139 GCTAGC G^CTAGC
367 GCTAGC
SphI 135 GCATGC GCATG^C
3277 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 130 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI AocI ApaI
ApoI AspHI AvaI AvaII BalI
BamHI BanI BanII BbvI BcnI
BfaI BglI BlpI BmyI BpmI
Bpu1102I BsaAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspLU11I BspMI
BsrBI BsrI BssKI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraI DraII DraIII DsaI EaeI
EarI Eco81I EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII XmaCI XmaI