################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AatII Acc65I AccI AccIII AclI AflIII AgeI AhdI Alw44I ApaLI AscI Asp718I AspEI AspI AviII AvrII BanI BbeI BbrPI BcgI BciVI BclI BglI BglII BlnI BsaAI BsaWI BseAI BsiEI BsiWI BspDI BspEI BspLU11I BsrBI BsrFI Bst1107I BstBI BstXI BstZ17I Cfr10I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I EcoT22I FbaI FseI FspI HpaI KasI KpnI KspI MluI MroI MspA1I NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 42 enzymes) # ################################### AflII 2549 CTTAAG C^TTAAG AocI 1173 CCTGAGG CC^T[ACGT]AGG AseI 1773 ATTAAT AT^TAAT AsnI 1773 ATTAAT AT^TAAT BamHI 2893 GGATCC G^GATCC BbvCI 189 GCTGAGG CCTCAGC(-5/-2) BfrI 2549 CTTAAG C^TTAAG BlpI 2604 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2604 GCTCAGC GC^T[ACGT]AGC BsaI 198 GGTCTC GGTCTC(1/5) BseRI 1833 GAGGAG GAGGAG(10/8) BsmBI 1998 CGTCTC CGTCTC(1/5) Bsp1407I 436 TGTACA T^GTACA BspMI 1313 ACCTGC ACCTGC(4/8) BsrGI 436 TGTACA T^GTACA BssHII 71 GCGCGC G^CGCGC BssSI 520 CTCGTG CACGAG(-5/-1) BstAPI 62 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 981 GGTAACC G^GT[ACGT]ACC BstPI 981 GGTAACC G^GT[ACGT]ACC Bsu36I 1173 CCTGAGG CC^T[ACGT]AGG CelII 2604 GCTCAGC GC^T[ACGT]AGC DraI 680 TTTAAA TTT^AAA DraIII 2979 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG Eco81I 1173 CCTGAGG CC^T[ACGT]AGG EcoNI 720 CCTAAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 981 GGTAACC G^GT[ACGT]ACC EcoRI 2008 GAATTC G^AATTC EcoRV 216 GATATC GAT^ATC HincII 735 GTTGAC GT[CT]^[GA]AC HindII 735 GTTGAC GT[CT]^[GA]AC HindIII 1267 AAGCTT A^AGCTT MaeII 2980 ACGT A^CGT NdeI 2238 CATATG CA^TATG PflMI 3014 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 2930 AGGACCC [GA]G^G[AT]CC[CT] PshBI 1773 ATTAAT AT^TAAT SacI 988 GAGCTC GAGCT^C SphI 847 GCATGC GCATG^C SspBI 436 TGTACA T^GTACA StuI 1708 AGGCCT AGG^CCT Van91I 3014 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcyI 12 GGCGTC G[GA]^CG[CT]C 2922 GACGCC Aor51HI 1687 AGCGCT AGC^GCT 1698 AGCGCT ApaI 48 GGGCCC GGGCC^C 1126 GGGCCC AvaI 51 CCCGGG C^[CT]CG[GA]G 123 CCCGGG AvaII 2931 GGACC G^G[AT]CC 3366 GGACC BsaBI 1395 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2348 GATTATCATC BsaHI 12 GGCGTC G[GA]^CG[CT]C 2922 GACGCC BsmI 2094 GAATGC GAATGC(1/-1) 2654 GAATGC BsoBI 51 CCCGGG C^[CT]CG[GA]G 123 CCCGGG BspHI 874 TCATGA T^CATGA 1633 TCATGA DraII 1126 GGGCCCT [GA]G^G[ACGT]CC[CT] 2930 AGGACCC DsaI 153 CCGTGG C^C[GA][CT]GG 2144 CCGTGG Eco47III 1687 AGCGCT AGC^GCT 1698 AGCGCT EcoO109I 1126 GGGCCCT [GA]G^G[ACGT]CC[CT] 2930 AGGACCC EcoT14I 1814 CCTTGG C^C[AT][AT]GG 3541 CCTTGG Hin1I 12 GGCGTC G[GA]^CG[CT]C 2922 GACGCC MamI 1395 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 2348 GATTATCATC MfeI 740 CAATTG C^AATTG 1755 CAATTG MunI 740 CAATTG C^AATTG 1755 CAATTG PleI 3497 GACTC GAGTC(4/5) 3703 GACTC PstI 2637 CTGCAG CTGCA^G 3361 CTGCAG RcaI 874 TCATGA T^CATGA 1633 TCATGA SmaI 51 CCCGGG CCC^GGG 123 CCCGGG StyI 1814 CCTTGG C^C[AT][AT]GG 3541 CCTTGG XcmI 968 CCATCTTCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1885 CCAATGAGGGGCTGG XmaCI 51 CCCGGG C^CCGGG 123 CCCGGG XmaI 51 CCCGGG C^CCGGG 123 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 93 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI Asp700I AspHI BalI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI