##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflIII AgeI AhdI Alw44I ApaLI
AscI Asp718I AspEI AspI AviII
AvrII BanI BbeI BbrPI BcgI
BciVI BclI BglI BglII BlnI
BsaAI BsaWI BseAI BsiEI BsiWI
BspDI BspEI BspLU11I BsrBI BsrFI
Bst1107I BstBI BstXI BstZ17I Cfr10I
ClaI CpoI DrdI EagI Eam1105I
EclXI Eco52I EcoT22I FbaI FseI
FspI HpaI KasI KpnI KspI
MluI MroI MspA1I NaeI NarI
NcoI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI PvuII RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
XhoI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AflII 2549 CTTAAG C^TTAAG
AocI 1173 CCTGAGG CC^T[ACGT]AGG
AseI 1773 ATTAAT AT^TAAT
AsnI 1773 ATTAAT AT^TAAT
BamHI 2893 GGATCC G^GATCC
BbvCI 189 GCTGAGG CCTCAGC(-5/-2)
BfrI 2549 CTTAAG C^TTAAG
BlpI 2604 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2604 GCTCAGC GC^T[ACGT]AGC
BsaI 198 GGTCTC GGTCTC(1/5)
BseRI 1833 GAGGAG GAGGAG(10/8)
BsmBI 1998 CGTCTC CGTCTC(1/5)
Bsp1407I 436 TGTACA T^GTACA
BspMI 1313 ACCTGC ACCTGC(4/8)
BsrGI 436 TGTACA T^GTACA
BssHII 71 GCGCGC G^CGCGC
BssSI 520 CTCGTG CACGAG(-5/-1)
BstAPI 62 GCAGACCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 981 GGTAACC G^GT[ACGT]ACC
BstPI 981 GGTAACC G^GT[ACGT]ACC
Bsu36I 1173 CCTGAGG CC^T[ACGT]AGG
CelII 2604 GCTCAGC GC^T[ACGT]AGC
DraI 680 TTTAAA TTT^AAA
DraIII 2979 CACGTCGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1173 CCTGAGG CC^T[ACGT]AGG
EcoNI 720 CCTAAATTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 981 GGTAACC G^GT[ACGT]ACC
EcoRI 2008 GAATTC G^AATTC
EcoRV 216 GATATC GAT^ATC
HincII 735 GTTGAC GT[CT]^[GA]AC
HindII 735 GTTGAC GT[CT]^[GA]AC
HindIII 1267 AAGCTT A^AGCTT
MaeII 2980 ACGT A^CGT
NdeI 2238 CATATG CA^TATG
PflMI 3014 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 2930 AGGACCC [GA]G^G[AT]CC[CT]
PshBI 1773 ATTAAT AT^TAAT
SacI 988 GAGCTC GAGCT^C
SphI 847 GCATGC GCATG^C
SspBI 436 TGTACA T^GTACA
StuI 1708 AGGCCT AGG^CCT
Van91I 3014 CCATAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 12 GGCGTC G[GA]^CG[CT]C
2922 GACGCC
Aor51HI 1687 AGCGCT AGC^GCT
1698 AGCGCT
ApaI 48 GGGCCC GGGCC^C
1126 GGGCCC
AvaI 51 CCCGGG C^[CT]CG[GA]G
123 CCCGGG
AvaII 2931 GGACC G^G[AT]CC
3366 GGACC
BsaBI 1395 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2348 GATTATCATC
BsaHI 12 GGCGTC G[GA]^CG[CT]C
2922 GACGCC
BsmI 2094 GAATGC GAATGC(1/-1)
2654 GAATGC
BsoBI 51 CCCGGG C^[CT]CG[GA]G
123 CCCGGG
BspHI 874 TCATGA T^CATGA
1633 TCATGA
DraII 1126 GGGCCCT [GA]G^G[ACGT]CC[CT]
2930 AGGACCC
DsaI 153 CCGTGG C^C[GA][CT]GG
2144 CCGTGG
Eco47III 1687 AGCGCT AGC^GCT
1698 AGCGCT
EcoO109I 1126 GGGCCCT [GA]G^G[ACGT]CC[CT]
2930 AGGACCC
EcoT14I 1814 CCTTGG C^C[AT][AT]GG
3541 CCTTGG
Hin1I 12 GGCGTC G[GA]^CG[CT]C
2922 GACGCC
MamI 1395 GATCAGAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2348 GATTATCATC
MfeI 740 CAATTG C^AATTG
1755 CAATTG
MunI 740 CAATTG C^AATTG
1755 CAATTG
PleI 3497 GACTC GAGTC(4/5)
3703 GACTC
PstI 2637 CTGCAG CTGCA^G
3361 CTGCAG
RcaI 874 TCATGA T^CATGA
1633 TCATGA
SmaI 51 CCCGGG CCC^GGG
123 CCCGGG
StyI 1814 CCTTGG C^C[AT][AT]GG
3541 CCTTGG
XcmI 968 CCATCTTCTTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1885 CCAATGAGGGGCTGG
XmaCI 51 CCCGGG C^CCGGG
123 CCCGGG
XmaI 51 CCCGGG C^CCGGG
123 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI Asp700I AspHI
BalI BanII BbsI BbvI BcnI
BfaI BmyI BpmI BpuAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
RsaI SapI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII XmnI