################################## # NO CUT ENZYMES ( 65 enzymes) # ################################## AatII Acc65I AcyI AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI Asp718I AspEI BanI BbeI BbvCI BcgI BglII BsaBI BsaHI BsiWI BsmBI BspDI BstBI ClaI CpoI DrdI Eam1105I Eco47III FseI HaeII Hin1I HpaI KasI KpnI MamI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PvuI RsrII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SwaI TaqI TthHB8I XbaI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AclI 1645 AACGTT AA^CGTT AflII 4752 CTTAAG C^TTAAG Asp700I 4679 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspI 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 53 TGCGCA TGC^GCA AvrII 1704 CCTAGG C^CTAGG BalI 692 TGGCCA TGG^CCA BamHI 2806 GGATCC G^GATCC BbrPI 2913 CACGTG CAC^GTG BciVI 3378 GTATCC GTATCC(6/5) BclI 3104 TGATCA T^GATCA BfrI 4752 CTTAAG C^TTAAG BlnI 1704 CCTAGG C^CTAGG BlpI 1248 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1248 GCTGAGC GC^T[ACGT]AGC BsaI 4338 GGTCTC GGTCTC(1/5) BsmI 1105 GAATGC GAATGC(1/-1) BspLU11I 350 ACATGT A^CATGT BsrBI 69 GAGCGG CCGCTC(-3/-3) BsrDI 4358 CATTGC GCAATG(2/0) BssSI 2283 CACGAG CACGAG(-5/-1) Bst1107I 4526 GTATAC GTA^TAC BstZ17I 4526 GTATAC GTA^TAC CelII 1248 GCTGAGC GC^T[ACGT]AGC EagI 126 CGGCCG C^GGCCG EclXI 126 CGGCCG C^GGCCG Eco52I 126 CGGCCG C^GGCCG EcoRI 2346 GAATTC G^AATTC EcoRV 296 GATATC GAT^ATC EcoT22I 1107 ATGCAT ATGCA^T FbaI 3104 TGATCA T^GATCA FspI 53 TGCGCA TGC^GCA KspI 78 CCGCGG CCGC^GG MluNI 692 TGGCCA TGG^CCA MscI 692 TGGCCA TGG^CCA NcoI 665 CCATGG C^CATGG NdeI 1177 CATATG CA^TATG NsiI 1107 ATGCAT ATGCA^T PflFI 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 4793 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2913 CACGTG CAC^GTG PmlI 2913 CACGTG CAC^GTG PshAI 3439 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 1645 AACGTT AA^CGTT SacI 3952 GAGCTC GAGCT^C SacII 78 CCGCGG CCGC^GG SmaI 2044 CCCGGG CCC^GGG SphI 608 GCATGC GCATG^C StuI 890 AGGCCT AGG^CCT Tth111I 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 4793 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XmaCI 2044 CCCGGG C^CCGGG XmaI 2044 CCCGGG C^CCGGG XmnI 4679 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccIII 205 TCCGGA T^CCGGA 3647 TCCGGA AflIII 350 ACATGT A^C[GA][CT]GT 2914 ACGTGT AseI 831 ATTAAT AT^TAAT 4967 ATTAAT AsnI 831 ATTAAT AT^TAAT 4967 ATTAAT AvaI 184 CTCGGG C^[CT]CG[GA]G 2044 CCCGGG BglI 174 GCCGTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4656 GCCTGCTAGGC BsaAI 534 CACGTA [CT]AC^GT[GA] 2913 CACGTG BseAI 205 TCCGGA T^CCGGA 3647 TCCGGA BsiEI 122 CGGTCG CG[GA][CT]^CG 126 CGGCCG BsoBI 184 CTCGGG C^[CT]CG[GA]G 2044 CCCGGG BspEI 205 TCCGGA T^CCGGA 3647 TCCGGA BstXI 665 CCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3843 CCATGCACCTGG BstYI 2806 GGATCC [GA]^GATC[CT] 3232 GGATCT EcoNI 643 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2992 CCTACAGCAGG HincII 2305 GTCAAC GT[CT]^[GA]AC 2435 GTCAAC HindII 2305 GTCAAC GT[CT]^[GA]AC 2435 GTCAAC HindIII 1136 AAGCTT A^AGCTT 1533 AAGCTT MflI 2806 GGATCC [GA]^GATC[CT] 3232 GGATCT MroI 205 TCCGGA T^CCGGA 3647 TCCGGA NaeI 179 GCCGGC GCC^GGC 189 GCCGGC NgoMIV 179 GCCGGC G^CCGGC 189 GCCGGC PshBI 831 ATTAAT AT^TAAT 4967 ATTAAT SexAI 3254 ACCAGGT A^CC[AT]GGT 4517 ACCTGGT SspI 834 AATATT AAT^ATT 4063 AATATT XcmI 2341 CCATTGAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2848 CCAAGTTTTCTCTGG XhoII 2806 GGATCC [GA]^GATC[CT] 3232 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 113 enzymes) # ######################################### AccI AccII AciI AcsI AfaI AluI AlwI AlwNI AocI ApoI AspHI AvaII BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspHI BspMI BsrFI BsrGI BsrI BssHII BssKI BstAPI BstEII BstF5I BstNI BstPI BstUI Bsu36I Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DraII DraIII DsaI EaeI EarI Eco81I EcoO109I EcoO65I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI