##################################
# NO CUT ENZYMES ( 65 enzymes) #
##################################
AatII Acc65I AcyI AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
Asp718I AspEI BanI BbeI BbvCI
BcgI BglII BsaBI BsaHI BsiWI
BsmBI BspDI BstBI ClaI CpoI
DrdI Eam1105I Eco47III FseI HaeII
Hin1I HpaI KasI KpnI MamI
MfeI MluI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PvuI RsrII SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SnaBI SpeI Sse8387I
SwaI TaqI TthHB8I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AclI 1645 AACGTT AA^CGTT
AflII 4752 CTTAAG C^TTAAG
Asp700I 4679 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 53 TGCGCA TGC^GCA
AvrII 1704 CCTAGG C^CTAGG
BalI 692 TGGCCA TGG^CCA
BamHI 2806 GGATCC G^GATCC
BbrPI 2913 CACGTG CAC^GTG
BciVI 3378 GTATCC GTATCC(6/5)
BclI 3104 TGATCA T^GATCA
BfrI 4752 CTTAAG C^TTAAG
BlnI 1704 CCTAGG C^CTAGG
BlpI 1248 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1248 GCTGAGC GC^T[ACGT]AGC
BsaI 4338 GGTCTC GGTCTC(1/5)
BsmI 1105 GAATGC GAATGC(1/-1)
BspLU11I 350 ACATGT A^CATGT
BsrBI 69 GAGCGG CCGCTC(-3/-3)
BsrDI 4358 CATTGC GCAATG(2/0)
BssSI 2283 CACGAG CACGAG(-5/-1)
Bst1107I 4526 GTATAC GTA^TAC
BstZ17I 4526 GTATAC GTA^TAC
CelII 1248 GCTGAGC GC^T[ACGT]AGC
EagI 126 CGGCCG C^GGCCG
EclXI 126 CGGCCG C^GGCCG
Eco52I 126 CGGCCG C^GGCCG
EcoRI 2346 GAATTC G^AATTC
EcoRV 296 GATATC GAT^ATC
EcoT22I 1107 ATGCAT ATGCA^T
FbaI 3104 TGATCA T^GATCA
FspI 53 TGCGCA TGC^GCA
KspI 78 CCGCGG CCGC^GG
MluNI 692 TGGCCA TGG^CCA
MscI 692 TGGCCA TGG^CCA
NcoI 665 CCATGG C^CATGG
NdeI 1177 CATATG CA^TATG
NsiI 1107 ATGCAT ATGCA^T
PflFI 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 4793 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2913 CACGTG CAC^GTG
PmlI 2913 CACGTG CAC^GTG
PshAI 3439 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1645 AACGTT AA^CGTT
SacI 3952 GAGCTC GAGCT^C
SacII 78 CCGCGG CCGC^GG
SmaI 2044 CCCGGG CCC^GGG
SphI 608 GCATGC GCATG^C
StuI 890 AGGCCT AGG^CCT
Tth111I 1473 GACGGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 4793 CCACTTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 2044 CCCGGG C^CCGGG
XmaI 2044 CCCGGG C^CCGGG
XmnI 4679 GAAAAGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccIII 205 TCCGGA T^CCGGA
3647 TCCGGA
AflIII 350 ACATGT A^C[GA][CT]GT
2914 ACGTGT
AseI 831 ATTAAT AT^TAAT
4967 ATTAAT
AsnI 831 ATTAAT AT^TAAT
4967 ATTAAT
AvaI 184 CTCGGG C^[CT]CG[GA]G
2044 CCCGGG
BglI 174 GCCGTGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4656 GCCTGCTAGGC
BsaAI 534 CACGTA [CT]AC^GT[GA]
2913 CACGTG
BseAI 205 TCCGGA T^CCGGA
3647 TCCGGA
BsiEI 122 CGGTCG CG[GA][CT]^CG
126 CGGCCG
BsoBI 184 CTCGGG C^[CT]CG[GA]G
2044 CCCGGG
BspEI 205 TCCGGA T^CCGGA
3647 TCCGGA
BstXI 665 CCATGGGCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3843 CCATGCACCTGG
BstYI 2806 GGATCC [GA]^GATC[CT]
3232 GGATCT
EcoNI 643 CCTCCTAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2992 CCTACAGCAGG
HincII 2305 GTCAAC GT[CT]^[GA]AC
2435 GTCAAC
HindII 2305 GTCAAC GT[CT]^[GA]AC
2435 GTCAAC
HindIII 1136 AAGCTT A^AGCTT
1533 AAGCTT
MflI 2806 GGATCC [GA]^GATC[CT]
3232 GGATCT
MroI 205 TCCGGA T^CCGGA
3647 TCCGGA
NaeI 179 GCCGGC GCC^GGC
189 GCCGGC
NgoMIV 179 GCCGGC G^CCGGC
189 GCCGGC
PshBI 831 ATTAAT AT^TAAT
4967 ATTAAT
SexAI 3254 ACCAGGT A^CC[AT]GGT
4517 ACCTGGT
SspI 834 AATATT AAT^ATT
4063 AATATT
XcmI 2341 CCATTGAATTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2848 CCAAGTTTTCTCTGG
XhoII 2806 GGATCC [GA]^GATC[CT]
3232 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AcsI AfaI
AluI AlwI AlwNI AocI ApoI
AspHI AvaII BanII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspHI BspMI BsrFI BsrGI
BsrI BssHII BssKI BstAPI BstEII
BstF5I BstNI BstPI BstUI Bsu36I
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraI DraII DraIII DsaI
EaeI EarI Eco81I EcoO109I EcoO65I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
SspBI StyI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI