################################## # NO CUT ENZYMES ( 83 enzymes) # ################################## AatII Acc65I AclI AcyI AgeI Alw44I AlwNI AocI ApaI ApaLI AscI Asp718I AspI AviII AvrII BamHI BbeI BbrPI BglI BlnI BsaAI BsaHI BsiWI BsmBI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstPI BstXI Bsu36I Cfr10I CpoI EagI EclXI Eco52I Eco81I EcoO65I EcoRI EcoRV FseI FspI HgaI Hin1I HincII HindII HpaI KasI KpnI MluI NaeI NarI NgoMIV NheI NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI Psp1406I RsrII SacI SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI Sse8387I SwaI Tth111I XhoI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### AccI 3874 GTATAC GT^[AC][GT]AC AccIII 3773 TCCGGA T^CCGGA AhdI 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AseI 3660 ATTAAT AT^TAAT AsnI 3660 ATTAAT AT^TAAT AspEI 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 35 TGGCCA TGG^CCA BbvCI 374 GCTGAGG CCTCAGC(-5/-2) BcgI 419 GCATGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3425 GGATAC GTATCC(6/5) BclI 3095 TGATCA T^GATCA BsaBI 3093 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 3773 TCCGGA [AT]^CCGG[AT] BseAI 3773 TCCGGA T^CCGGA BsiEI 468 CGATCG CG[GA][CT]^CG BsmI 960 GAATGC GAATGC(1/-1) BspDI 470 ATCGAT AT^CGAT BspEI 3773 TCCGGA T^CCGGA BspHI 641 TCATGA T^CATGA Bst1107I 3874 GTATAC GTA^TAC BstZ17I 3874 GTATAC GTA^TAC ClaI 470 ATCGAT AT^CGAT DrdI 1010 GACATTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EaeI 35 TGGCCA [CT]^GGCC[GA] Eam1105I 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 3081 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 3095 TGATCA T^GATCA MaeII 1284 ACGT A^CGT MamI 3093 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 35 TGGCCA TGG^CCA MroI 3773 TCCGGA T^CCGGA MscI 35 TGGCCA TGG^CCA NcoI 2400 CCATGG C^CATGG NdeI 3300 CATATG CA^TATG PshAI 2483 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PshBI 3660 ATTAAT AT^TAAT PstI 1810 CTGCAG CTGCA^G PvuI 468 CGATCG CGAT^CG RcaI 641 TCATGA T^CATGA ScaI 2105 AGTACT AGT^ACT SpeI 605 ACTAGT A^CTAGT SphI 3509 GCATGC GCATG^C XcmI 3796 CCAGCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 38 enzymes) # ################################### AflII 2846 CTTAAG C^TTAAG 2892 CTTAAG AflIII 3501 ACATGT A^C[GA][CT]GT 3515 ACATGT Aor51HI 827 AGCGCT AGC^GCT 3463 AGCGCT Asp700I 743 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3072 GAATGATTTC AspHI 648 GTGCTC G[AT]GC[AT]^C 3757 GAGCAC AvaI 74 CCCGGG C^[CT]CG[GA]G 523 CCCGGG BanI 105 GGCACC G^G[CT][GA]CC 2851 GGCACC BanII 70 GAGCCC G[GA]GC[CT]^C 2526 GAGCCC BbsI 544 GAAGAC GAAGAC(2/6) 3861 GAAGAC BfrI 2846 CTTAAG C^TTAAG 2892 CTTAAG BglII 745 AGATCT A^GATCT 3103 AGATCT BlpI 86 GCTGAGC GC^T[ACGT]AGC 1524 GCTTAGC Bpu1102I 86 GCTGAGC GC^T[ACGT]AGC 1524 GCTTAGC BpuAI 544 GAAGAC GAAGAC(2/6) 3861 GAAGAC BsgI 3226 CTGCAC GTGCAG(16/14) 901 GTGCAG BsiHKAI 648 GTGCTC G[AT]GC[AT]^C 3757 GAGCAC BsoBI 74 CCCGGG C^[CT]CG[GA]G 523 CCCGGG BspLU11I 3501 ACATGT A^CATGT 3515 ACATGT BstF5I 3092 GGATG GGATG(2/0) 3838 GGATG CelII 86 GCTGAGC GC^T[ACGT]AGC 1524 GCTTAGC DraIII 2817 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG 3804 CACATGGTG Eco47III 827 AGCGCT AGC^GCT 3463 AGCGCT EcoT22I 3034 ATGCAT ATGCA^T 3635 ATGCAT FokI 3092 GGATG GGATG(9/13) 3838 GGATG KspI 117 CCGCGG CCGC^GG 3005 CCGCGG MfeI 2601 CAATTG C^AATTG 2654 CAATTG MunI 2601 CAATTG C^AATTG 2654 CAATTG NsiI 3034 ATGCAT ATGCA^T 3635 ATGCAT PflMI 2255 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3803 CCACATGGTGG PvuII 241 CAGCTG CAG^CTG 564 CAGCTG SacII 117 CCGCGG CCGC^GG 3005 CCGCGG SmaI 74 CCCGGG CCC^GGG 523 CCCGGG SspI 1402 AATATT AAT^ATT 2001 AATATT Van91I 2255 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3803 CCACATGGTGG XbaI 192 TCTAGA T^CTAGA 1922 TCTAGA XmaCI 74 CCCGGG C^CCGGG 523 CCCGGG XmaI 74 CCCGGG C^CCGGG 523 CCCGGG XmnI 743 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3072 GAATGATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI ApoI AvaII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI HaeII HaeIII HapII HhaI HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII