##################################
# NO CUT ENZYMES ( 83 enzymes) #
##################################
AatII Acc65I AclI AcyI AgeI
Alw44I AlwNI AocI ApaI ApaLI
AscI Asp718I AspI AviII AvrII
BamHI BbeI BbrPI BglI BlnI
BsaAI BsaHI BsiWI BsmBI BsrBI
BsrFI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI Bsu36I Cfr10I
CpoI EagI EclXI Eco52I Eco81I
EcoO65I EcoRI EcoRV FseI FspI
HgaI Hin1I HincII HindII HpaI
KasI KpnI MluI NaeI NarI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I RsrII SacI
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SwaI Tth111I XhoI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 3874 GTATAC GT^[AC][GT]AC
AccIII 3773 TCCGGA T^CCGGA
AhdI 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AseI 3660 ATTAAT AT^TAAT
AsnI 3660 ATTAAT AT^TAAT
AspEI 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 35 TGGCCA TGG^CCA
BbvCI 374 GCTGAGG CCTCAGC(-5/-2)
BcgI 419 GCATGGCAGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3425 GGATAC GTATCC(6/5)
BclI 3095 TGATCA T^GATCA
BsaBI 3093 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 3773 TCCGGA [AT]^CCGG[AT]
BseAI 3773 TCCGGA T^CCGGA
BsiEI 468 CGATCG CG[GA][CT]^CG
BsmI 960 GAATGC GAATGC(1/-1)
BspDI 470 ATCGAT AT^CGAT
BspEI 3773 TCCGGA T^CCGGA
BspHI 641 TCATGA T^CATGA
Bst1107I 3874 GTATAC GTA^TAC
BstZ17I 3874 GTATAC GTA^TAC
ClaI 470 ATCGAT AT^CGAT
DrdI 1010 GACATTTATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 35 TGGCCA [CT]^GGCC[GA]
Eam1105I 2436 GACCGCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3081 CCTGATTAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 3095 TGATCA T^GATCA
MaeII 1284 ACGT A^CGT
MamI 3093 GATGATCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 35 TGGCCA TGG^CCA
MroI 3773 TCCGGA T^CCGGA
MscI 35 TGGCCA TGG^CCA
NcoI 2400 CCATGG C^CATGG
NdeI 3300 CATATG CA^TATG
PshAI 2483 GACATTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 3660 ATTAAT AT^TAAT
PstI 1810 CTGCAG CTGCA^G
PvuI 468 CGATCG CGAT^CG
RcaI 641 TCATGA T^CATGA
ScaI 2105 AGTACT AGT^ACT
SpeI 605 ACTAGT A^CTAGT
SphI 3509 GCATGC GCATG^C
XcmI 3796 CCAGCAACCACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 38 enzymes) #
###################################
AflII 2846 CTTAAG C^TTAAG
2892 CTTAAG
AflIII 3501 ACATGT A^C[GA][CT]GT
3515 ACATGT
Aor51HI 827 AGCGCT AGC^GCT
3463 AGCGCT
Asp700I 743 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3072 GAATGATTTC
AspHI 648 GTGCTC G[AT]GC[AT]^C
3757 GAGCAC
AvaI 74 CCCGGG C^[CT]CG[GA]G
523 CCCGGG
BanI 105 GGCACC G^G[CT][GA]CC
2851 GGCACC
BanII 70 GAGCCC G[GA]GC[CT]^C
2526 GAGCCC
BbsI 544 GAAGAC GAAGAC(2/6)
3861 GAAGAC
BfrI 2846 CTTAAG C^TTAAG
2892 CTTAAG
BglII 745 AGATCT A^GATCT
3103 AGATCT
BlpI 86 GCTGAGC GC^T[ACGT]AGC
1524 GCTTAGC
Bpu1102I 86 GCTGAGC GC^T[ACGT]AGC
1524 GCTTAGC
BpuAI 544 GAAGAC GAAGAC(2/6)
3861 GAAGAC
BsgI 3226 CTGCAC GTGCAG(16/14)
901 GTGCAG
BsiHKAI 648 GTGCTC G[AT]GC[AT]^C
3757 GAGCAC
BsoBI 74 CCCGGG C^[CT]CG[GA]G
523 CCCGGG
BspLU11I 3501 ACATGT A^CATGT
3515 ACATGT
BstF5I 3092 GGATG GGATG(2/0)
3838 GGATG
CelII 86 GCTGAGC GC^T[ACGT]AGC
1524 GCTTAGC
DraIII 2817 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
3804 CACATGGTG
Eco47III 827 AGCGCT AGC^GCT
3463 AGCGCT
EcoT22I 3034 ATGCAT ATGCA^T
3635 ATGCAT
FokI 3092 GGATG GGATG(9/13)
3838 GGATG
KspI 117 CCGCGG CCGC^GG
3005 CCGCGG
MfeI 2601 CAATTG C^AATTG
2654 CAATTG
MunI 2601 CAATTG C^AATTG
2654 CAATTG
NsiI 3034 ATGCAT ATGCA^T
3635 ATGCAT
PflMI 2255 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3803 CCACATGGTGG
PvuII 241 CAGCTG CAG^CTG
564 CAGCTG
SacII 117 CCGCGG CCGC^GG
3005 CCGCGG
SmaI 74 CCCGGG CCC^GGG
523 CCCGGG
SspI 1402 AATATT AAT^ATT
2001 AATATT
Van91I 2255 CCAAATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3803 CCACATGGTGG
XbaI 192 TCTAGA T^CTAGA
1922 TCTAGA
XmaCI 74 CCCGGG C^CCGGG
523 CCCGGG
XmaI 74 CCCGGG C^CCGGG
523 CCCGGG
XmnI 743 GAAGATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3072 GAATGATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI ApoI AvaII BbvI BcnI
BfaI BmyI BpmI BsaI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I Bsp1407I BspMI BsrDI BsrGI
BsrI BssKI BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraI DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI HinP1I HindIII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII