################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## Acc65I AccIII AclI AgeI ApaI AscI AseI AsnI Asp718I AviII AvrII BamHI BbrPI BlnI BlpI Bpu1102I BsaAI BseAI BsiWI Bsp1407I BspDI BspEI BspHI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstXI BstZ17I CelII ClaI CpoI EcoNI EcoO65I EcoRV FseI FspI HpaI KpnI MfeI MluI MroI MunI NheI NotI NruI NspV PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI XcmI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 2448 GACGTC GACGT^C AflII 3907 CTTAAG C^TTAAG AflIII 2957 ACATGT A^C[GA][CT]GT AhdI 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 3857 GTGCAC G^TGCAC Aor51HI 4387 AGCGCT AGC^GCT ApaLI 3857 GTGCAC G^TGCAC AspEI 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 3691 TGGCCA TGG^CCA BbeI 668 GGCGCC GGCGC^C BbvCI 1594 CCTCAGC CCTCAGC(-5/-2) BclI 2372 TGATCA T^GATCA BfrI 3907 CTTAAG C^TTAAG BglII 4284 AGATCT A^GATCT BsaBI 3648 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BspLU11I 2957 ACATGT A^CATGT BsrBI 2879 GAGCGG CCGCTC(-3/-3) BssSI 2385 CTCGTG CACGAG(-5/-1) BstAPI 225 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 3972 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG DrdI 4318 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eam1105I 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 4387 AGCGCT AGC^GCT EcoRI 2970 GAATTC G^AATTC EcoT22I 4602 ATGCAT ATGCA^T FbaI 2372 TGATCA T^GATCA KasI 668 GGCGCC G^GCGCC MamI 3648 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MluNI 3691 TGGCCA TGG^CCA MscI 3691 TGGCCA TGG^CCA NarI 668 GGCGCC GG^CGCC NdeI 2161 CATATG CA^TATG NsiI 4602 ATGCAT ATGCA^T PacI 2286 TTAATTAA TTAAT^TAA PaeR7I 75 CTCGAG C^TCGAG PflFI 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 2058 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 394 AGGACCT [GA]G^G[AT]CC[CT] SacI 2382 GAGCTC GAGCT^C SapI 771 GCTCTTC GCTCTTC(1/4) SfoI 668 GGCGCC GGC^GCC TaqI 76 TCGA T^CGA Tth111I 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC TthHB8I 76 TCGA T^CGA Van91I 2058 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1020 TCTAGA T^CTAGA XhoI 75 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 31 enzymes) # ################################### AccI 261 GTAGAC GT^[AC][GT]AC 3755 GTAGAC AlwNI 226 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG 4474 CAGACTCTG AocI 2556 CCTCAGG CC^T[ACGT]AGG 2823 CCTAAGG Asp700I 3096 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3417 GAATTGGTTC AspHI 2382 GAGCTC G[AT]GC[AT]^C 3857 GTGCAC BcgI 1604 CGAGAGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 234 GCAGCATCATCG BciVI 951 GGATAC GTATCC(6/5) 2847 GTATCC BsaI 759 GAGACC GGTCTC(1/5) 278 GGTCTC BsaWI 452 TCCGGT [AT]^CCGG[AT] 2402 ACCGGA BsgI 1210 GTGCAG GTGCAG(16/14) 3829 GTGCAG BsiEI 529 CGGCCG CG[GA][CT]^CG 721 CGGCCG BsiHKAI 2382 GAGCTC G[AT]GC[AT]^C 3857 GTGCAC BsmBI 2110 CGTCTC CGTCTC(1/5) 3483 CGTCTC BsmI 1520 GAATGC GAATGC(1/-1) 4600 GAATGC BspMI 1238 ACCTGC ACCTGC(4/8) 4020 GCAGGT BsrDI 2273 CATTGC GCAATG(2/0) 3287 CATTGC Bsu36I 2556 CCTCAGG CC^T[ACGT]AGG 2823 CCTAAGG DraII 394 AGGACCT [GA]G^G[ACGT]CC[CT] 3743 AGGCCCC EagI 529 CGGCCG C^GGCCG 721 CGGCCG EclXI 529 CGGCCG C^GGCCG 721 CGGCCG Eco52I 529 CGGCCG C^GGCCG 721 CGGCCG Eco81I 2556 CCTCAGG CC^T[ACGT]AGG 2823 CCTAAGG EcoO109I 394 AGGACCT [GA]G^G[ACGT]CC[CT] 3743 AGGCCCC HaeII 668 GGCGCC [GA]GCGC^[CT] 4387 AGCGCT HincII 2418 GTTGAC GT[CT]^[GA]AC 3177 GTTGAC HindII 2418 GTTGAC GT[CT]^[GA]AC 3177 GTTGAC MslI 2498 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 4530 CATTGTTATG NcoI 250 CCATGG C^CATGG 3688 CCATGG ScaI 1608 AGTACT AGT^ACT 3074 AGTACT SfiI 150 GGCCAGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC 298 GGCCGCCCCGGCC XmnI 3096 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3417 GAATTGGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AvaI AvaII BanI BanII BbsI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HindIII HinfI HpaII HphI ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MvnI MwoI NaeI NciI NdeII NgoMIV NlaIII NlaIV NspI PleI PstI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XhoII