##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AgeI ApaI
AscI AseI AsnI Asp718I AviII
AvrII BamHI BbrPI BlnI BlpI
Bpu1102I BsaAI BseAI BsiWI Bsp1407I
BspDI BspEI BspHI BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstXI
BstZ17I CelII ClaI CpoI EcoNI
EcoO65I EcoRV FseI FspI HpaI
KpnI MfeI MluI MroI MunI
NheI NotI NruI NspV PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SalI
SbfI SexAI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI XcmI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 2448 GACGTC GACGT^C
AflII 3907 CTTAAG C^TTAAG
AflIII 2957 ACATGT A^C[GA][CT]GT
AhdI 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3857 GTGCAC G^TGCAC
Aor51HI 4387 AGCGCT AGC^GCT
ApaLI 3857 GTGCAC G^TGCAC
AspEI 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 3691 TGGCCA TGG^CCA
BbeI 668 GGCGCC GGCGC^C
BbvCI 1594 CCTCAGC CCTCAGC(-5/-2)
BclI 2372 TGATCA T^GATCA
BfrI 3907 CTTAAG C^TTAAG
BglII 4284 AGATCT A^GATCT
BsaBI 3648 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspLU11I 2957 ACATGT A^CATGT
BsrBI 2879 GAGCGG CCGCTC(-3/-3)
BssSI 2385 CTCGTG CACGAG(-5/-1)
BstAPI 225 GCAGTAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 3972 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 4318 GACCTCTGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 264 GACCAGTCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 4387 AGCGCT AGC^GCT
EcoRI 2970 GAATTC G^AATTC
EcoT22I 4602 ATGCAT ATGCA^T
FbaI 2372 TGATCA T^GATCA
KasI 668 GGCGCC G^GCGCC
MamI 3648 GATCATTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 3691 TGGCCA TGG^CCA
MscI 3691 TGGCCA TGG^CCA
NarI 668 GGCGCC GG^CGCC
NdeI 2161 CATATG CA^TATG
NsiI 4602 ATGCAT ATGCA^T
PacI 2286 TTAATTAA TTAAT^TAA
PaeR7I 75 CTCGAG C^TCGAG
PflFI 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 2058 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 394 AGGACCT [GA]G^G[AT]CC[CT]
SacI 2382 GAGCTC GAGCT^C
SapI 771 GCTCTTC GCTCTTC(1/4)
SfoI 668 GGCGCC GGC^GCC
TaqI 76 TCGA T^CGA
Tth111I 1917 GACTTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 76 TCGA T^CGA
Van91I 2058 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1020 TCTAGA T^CTAGA
XhoI 75 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 261 GTAGAC GT^[AC][GT]AC
3755 GTAGAC
AlwNI 226 CAGTAGCTG CAG[ACGT][ACGT][ACGT]^CTG
4474 CAGACTCTG
AocI 2556 CCTCAGG CC^T[ACGT]AGG
2823 CCTAAGG
Asp700I 3096 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3417 GAATTGGTTC
AspHI 2382 GAGCTC G[AT]GC[AT]^C
3857 GTGCAC
BcgI 1604 CGAGAGTACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
234 GCAGCATCATCG
BciVI 951 GGATAC GTATCC(6/5)
2847 GTATCC
BsaI 759 GAGACC GGTCTC(1/5)
278 GGTCTC
BsaWI 452 TCCGGT [AT]^CCGG[AT]
2402 ACCGGA
BsgI 1210 GTGCAG GTGCAG(16/14)
3829 GTGCAG
BsiEI 529 CGGCCG CG[GA][CT]^CG
721 CGGCCG
BsiHKAI 2382 GAGCTC G[AT]GC[AT]^C
3857 GTGCAC
BsmBI 2110 CGTCTC CGTCTC(1/5)
3483 CGTCTC
BsmI 1520 GAATGC GAATGC(1/-1)
4600 GAATGC
BspMI 1238 ACCTGC ACCTGC(4/8)
4020 GCAGGT
BsrDI 2273 CATTGC GCAATG(2/0)
3287 CATTGC
Bsu36I 2556 CCTCAGG CC^T[ACGT]AGG
2823 CCTAAGG
DraII 394 AGGACCT [GA]G^G[ACGT]CC[CT]
3743 AGGCCCC
EagI 529 CGGCCG C^GGCCG
721 CGGCCG
EclXI 529 CGGCCG C^GGCCG
721 CGGCCG
Eco52I 529 CGGCCG C^GGCCG
721 CGGCCG
Eco81I 2556 CCTCAGG CC^T[ACGT]AGG
2823 CCTAAGG
EcoO109I 394 AGGACCT [GA]G^G[ACGT]CC[CT]
3743 AGGCCCC
HaeII 668 GGCGCC [GA]GCGC^[CT]
4387 AGCGCT
HincII 2418 GTTGAC GT[CT]^[GA]AC
3177 GTTGAC
HindII 2418 GTTGAC GT[CT]^[GA]AC
3177 GTTGAC
MslI 2498 CACTGAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
4530 CATTGTTATG
NcoI 250 CCATGG C^CATGG
3688 CCATGG
ScaI 1608 AGTACT AGT^ACT
3074 AGTACT
SfiI 150 GGCCAGCCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
298 GGCCGCCCCGGCC
XmnI 3096 GAAAAACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3417 GAATTGGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AvaI AvaII
BanI BanII BbsI BbvI BcnI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EaeI
EarI EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HindIII HinfI HpaII
HphI ItaI Ksp632I KspI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NaeI NciI NdeII
NgoMIV NlaIII NlaIV NspI PleI
PstI PvuII RsaI SacII Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII