##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII AclI AflII AgeI AscI
AseI AsnI BamHI BcgI BclI
BfrI BglII BsaBI BsmBI BsmI
Bsp1407I BspLU11I BsrGI BssHII Bst1107I
BstBI BstZ17I CpoI DrdI EagI
EclXI Eco52I EcoRI FbaI FseI
HindIII KspI MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI SspBI SspI SwaI XbaI
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 3854 GTAGAC GT^[AC][GT]AC
AccIII 1377 TCCGGA T^CCGGA
AhdI 2513 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3491 CCTGAGG CC^T[ACGT]AGG
AspEI 2513 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 2639 TGCGCA TGC^GCA
AvrII 3964 CCTAGG C^CTAGG
BciVI 3034 GGATAC GTATCC(6/5)
BlnI 3964 CCTAGG C^CTAGG
BlpI 2837 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2837 GCTCAGC GC^T[ACGT]AGC
BsaWI 1377 TCCGGA [AT]^CCGG[AT]
BseAI 1377 TCCGGA T^CCGGA
BsiEI 1846 CGACCG CG[GA][CT]^CG
BsiWI 367 CGTACG C^GTACG
BspDI 1364 ATCGAT AT^CGAT
BspEI 1377 TCCGGA T^CCGGA
BspHI 1788 TCATGA T^CATGA
BsrBI 2576 GAGCGG CCGCTC(-3/-3)
BsrDI 631 CATTGC GCAATG(2/0)
BsrFI 2284 GCCGGT [GA]^CCGG[CT]
BssSI 1425 CACGAG CACGAG(-5/-1)
BstAPI 1445 GCAAAGGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 3013 GGTGACC G^GT[ACGT]ACC
BstPI 3013 GGTGACC G^GT[ACGT]ACC
Bsu36I 3491 CCTGAGG CC^T[ACGT]AGG
CelII 2837 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2284 GCCGGT [GA]^CCGG[CT]
ClaI 1364 ATCGAT AT^CGAT
DraIII 1425 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 2513 GACGCTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 3491 CCTGAGG CC^T[ACGT]AGG
EcoO65I 3013 GGTGACC G^GT[ACGT]ACC
EcoRV 1367 GATATC GAT^ATC
EcoT22I 3252 ATGCAT ATGCA^T
FspI 2639 TGCGCA TGC^GCA
HpaI 4274 GTTAAC GTT^AAC
MroI 1377 TCCGGA T^CCGGA
NcoI 801 CCATGG C^CATGG
NsiI 3252 ATGCAT ATGCA^T
NspI 1615 GCATGT [GA]CATG^[CT]
RcaI 1788 TCATGA T^CATGA
SapI 1201 GCTCTTC GCTCTTC(1/4)
SbfI 3352 CCTGCAGG CCTGCA^GG
ScaI 517 AGTACT AGT^ACT
SpeI 203 ACTAGT A^CTAGT
Sse8387I 3352 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 27 GAATTT [GA]^AATT[CT]
4221 GAATTT
AcyI 1899 GGCGCC G[GA]^CG[CT]C
3558 GGCGCC
AflIII 1161 ACGTGT A^C[GA][CT]GT
1661 ACGTGT
Alw44I 1157 GTGCAC G^TGCAC
3774 GTGCAC
ApaLI 1157 GTGCAC G^TGCAC
3774 GTGCAC
ApoI 27 GAATTT [GA]^AATT[CT]
4221 GAATTT
BbeI 1899 GGCGCC GGCGC^C
3558 GGCGCC
BbvCI 869 GCTGAGG CCTCAGC(-5/-2)
3977 GCTGAGG
BsaHI 1899 GGCGCC G[GA]^CG[CT]C
3558 GGCGCC
BstYI 1176 AGATCC [GA]^GATC[CT]
3400 AGATCC
DraI 4438 TTTAAA TTT^AAA
4449 TTTAAA
Hin1I 1899 GGCGCC G[GA]^CG[CT]C
3558 GGCGCC
HincII 1766 GTCAAC GT[CT]^[GA]AC
4274 GTTAAC
HindII 1766 GTCAAC GT[CT]^[GA]AC
4274 GTTAAC
KasI 1899 GGCGCC G^GCGCC
3558 GGCGCC
MflI 1176 AGATCC [GA]^GATC[CT]
3400 AGATCC
NarI 1899 GGCGCC GG^CGCC
3558 GGCGCC
SacI 919 GAGCTC GAGCT^C
3002 GAGCTC
SfoI 1899 GGCGCC GGC^GCC
3558 GGCGCC
XcmI 2355 CCATCAAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
4214 CCAATGGGAATTTGG
XhoII 1176 AGATCC [GA]^GATC[CT]
3400 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
Acc65I AccII AciI AfaI AluI
AlwI AlwNI Aor51HI ApaI Asp700I
Asp718I AspHI AspI AvaI AvaII
BalI BanI BanII BbrPI BbsI
BbvI BcnI BfaI BglI BmyI
BpmI BpuAI BsaAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstUI
BstXI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI KpnI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflFI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I TthHB8I
Van91I XmnI