################################## # NO CUT ENZYMES ( 56 enzymes) # ################################## Acc65I AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AspI AvrII BbrPI BclI BglI BlnI BsaI BsiWI BspDI BspHI ClaI CpoI DrdI Eam1105I Eco47III FbaI FseI KpnI MluI NheI NotI PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SfiI SgrAI SnaBI SphI Sse8387I SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 56 enzymes) # ################################### AatII 578 GACGTC GACGT^C AccI 4086 GTATAC GT^[AC][GT]AC AccIII 34 TCCGGA T^CCGGA AflII 1298 CTTAAG C^TTAAG AflIII 4199 ACATGT A^C[GA][CT]GT Alw44I 489 GTGCAC G^TGCAC AlwNI 551 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG ApaI 3459 GGGCCC GGGCC^C ApaLI 489 GTGCAC G^TGCAC AviII 1821 TGCGCA TGC^GCA BamHI 3563 GGATCC G^GATCC BbvCI 4073 GCTGAGG CCTCAGC(-5/-2) BcgI 2394 GCAAGGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2447 GTATCC GTATCC(6/5) BfrI 1298 CTTAAG C^TTAAG BsaAI 3845 TACGTG [CT]AC^GT[GA] BsaWI 34 TCCGGA [AT]^CCGG[AT] BseAI 34 TCCGGA T^CCGGA Bsp1407I 2279 TGTACA T^GTACA BspEI 34 TCCGGA T^CCGGA BspLU11I 4199 ACATGT A^CATGT BsrGI 2279 TGTACA T^GTACA Bst1107I 4086 GTATAC GTA^TAC BstAPI 4101 GCATGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1220 TTCGAA TT^CGAA BstXI 1119 CCATCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 4086 GTATAC GTA^TAC EcoNI 131 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoRI 1452 GAATTC G^AATTC EcoRV 2399 GATATC GAT^ATC EcoT22I 3164 ATGCAT ATGCA^T FspI 1821 TGCGCA TGC^GCA HpaI 3429 GTTAAC GTT^AAC MfeI 3222 CAATTG C^AATTG MroI 34 TCCGGA T^CCGGA MunI 3222 CAATTG C^AATTG NaeI 466 GCCGGC GCC^GGC NcoI 3714 CCATGG C^CATGG NdeI 3954 CATATG CA^TATG NgoMIV 466 GCCGGC G^CCGGC NruI 692 TCGCGA TCG^CGA NsiI 3164 ATGCAT ATGCA^T NspV 1220 TTCGAA TT^CGAA PflMI 3596 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 1854 AGGTCCC [GA]G^G[AT]CC[CT] PstI 895 CTGCAG CTGCA^G SacI 767 GAGCTC GAGCT^C SapI 769 GCTCTTC GCTCTTC(1/4) ScaI 858 AGTACT AGT^ACT SfuI 1220 TTCGAA TT^CGAA SmaI 70 CCCGGG CCC^GGG SspBI 2279 TGTACA T^GTACA Van91I 3596 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 2251 TCTAGA T^CTAGA XmaCI 70 CCCGGG C^CCGGG XmaI 70 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AocI 1429 CCTGAGG CC^T[ACGT]AGG 4178 CCTAAGG AvaI 70 CCCGGG C^[CT]CG[GA]G 764 CCCGAG BglII 990 AGATCT A^GATCT 3787 AGATCT BsgI 3138 GTGCAG GTGCAG(16/14) 3848 GTGCAG BsmBI 41 CGTCTC CGTCTC(1/5) 622 GAGACG BsoBI 70 CCCGGG C^[CT]CG[GA]G 764 CCCGAG BsrBI 164 CCGCTC CCGCTC(-3/-3) 1486 CCGCTC BssSI 243 CACGAG CACGAG(-5/-1) 200 CTCGTG BstEII 1196 GGTAACC G^GT[ACGT]ACC 3394 GGTTACC BstPI 1196 GGTAACC G^GT[ACGT]ACC 3394 GGTTACC Bsu36I 1429 CCTGAGG CC^T[ACGT]AGG 4178 CCTAAGG DraI 2582 TTTAAA TTT^AAA 3319 TTTAAA DraIII 1147 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG 1938 CACCTAGTG EagI 305 CGGCCG C^GGCCG 913 CGGCCG EarI 770 CTCTTC CTCTTC(1/4) 229 GAAGAG EclXI 305 CGGCCG C^GGCCG 913 CGGCCG Eco52I 305 CGGCCG C^GGCCG 913 CGGCCG Eco81I 1429 CCTGAGG CC^T[ACGT]AGG 4178 CCTAAGG EcoO65I 1196 GGTAACC G^GT[ACGT]ACC 3394 GGTTACC HindIII 806 AAGCTT A^AGCTT 3324 AAGCTT Ksp632I 770 CTCTTC CTCTTC(1/4) 229 GAAGAG KspI 428 CCGCGG CCGC^GG 477 CCGCGG SacII 428 CCGCGG CCGC^GG 477 CCGCGG SexAI 1374 ACCTGGT A^CC[AT]GGT 3130 ACCTGGT SpeI 3333 ACTAGT A^CTAGT 3703 ACTAGT SspI 2984 AATATT AAT^ATT 4372 AATATT StuI 1609 AGGCCT AGG^CCT 2768 AGGCCT ######################################### # 3 OR MORE CUT ENZYMES ( 119 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaII BalI BanI BanII BbeI BbsI BbvI BcnI BfaI BlpI BmyI BpmI Bpu1102I BpuAI BsaBI BsaHI BsaJI BseRI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI Bsp1286I BspMI BsrDI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII