##################################
# NO CUT ENZYMES ( 56 enzymes) #
##################################
Acc65I AclI AgeI AhdI Aor51HI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AvrII BbrPI BclI
BglI BlnI BsaI BsiWI BspDI
BspHI ClaI CpoI DrdI Eam1105I
Eco47III FbaI FseI KpnI MluI
NheI NotI PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI SfiI SgrAI SnaBI
SphI Sse8387I SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AatII 578 GACGTC GACGT^C
AccI 4086 GTATAC GT^[AC][GT]AC
AccIII 34 TCCGGA T^CCGGA
AflII 1298 CTTAAG C^TTAAG
AflIII 4199 ACATGT A^C[GA][CT]GT
Alw44I 489 GTGCAC G^TGCAC
AlwNI 551 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaI 3459 GGGCCC GGGCC^C
ApaLI 489 GTGCAC G^TGCAC
AviII 1821 TGCGCA TGC^GCA
BamHI 3563 GGATCC G^GATCC
BbvCI 4073 GCTGAGG CCTCAGC(-5/-2)
BcgI 2394 GCAAGGATATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2447 GTATCC GTATCC(6/5)
BfrI 1298 CTTAAG C^TTAAG
BsaAI 3845 TACGTG [CT]AC^GT[GA]
BsaWI 34 TCCGGA [AT]^CCGG[AT]
BseAI 34 TCCGGA T^CCGGA
Bsp1407I 2279 TGTACA T^GTACA
BspEI 34 TCCGGA T^CCGGA
BspLU11I 4199 ACATGT A^CATGT
BsrGI 2279 TGTACA T^GTACA
Bst1107I 4086 GTATAC GTA^TAC
BstAPI 4101 GCATGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1220 TTCGAA TT^CGAA
BstXI 1119 CCATCTTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 4086 GTATAC GTA^TAC
EcoNI 131 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1452 GAATTC G^AATTC
EcoRV 2399 GATATC GAT^ATC
EcoT22I 3164 ATGCAT ATGCA^T
FspI 1821 TGCGCA TGC^GCA
HpaI 3429 GTTAAC GTT^AAC
MfeI 3222 CAATTG C^AATTG
MroI 34 TCCGGA T^CCGGA
MunI 3222 CAATTG C^AATTG
NaeI 466 GCCGGC GCC^GGC
NcoI 3714 CCATGG C^CATGG
NdeI 3954 CATATG CA^TATG
NgoMIV 466 GCCGGC G^CCGGC
NruI 692 TCGCGA TCG^CGA
NsiI 3164 ATGCAT ATGCA^T
NspV 1220 TTCGAA TT^CGAA
PflMI 3596 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1854 AGGTCCC [GA]G^G[AT]CC[CT]
PstI 895 CTGCAG CTGCA^G
SacI 767 GAGCTC GAGCT^C
SapI 769 GCTCTTC GCTCTTC(1/4)
ScaI 858 AGTACT AGT^ACT
SfuI 1220 TTCGAA TT^CGAA
SmaI 70 CCCGGG CCC^GGG
SspBI 2279 TGTACA T^GTACA
Van91I 3596 CCAAAGTTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2251 TCTAGA T^CTAGA
XmaCI 70 CCCGGG C^CCGGG
XmaI 70 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AocI 1429 CCTGAGG CC^T[ACGT]AGG
4178 CCTAAGG
AvaI 70 CCCGGG C^[CT]CG[GA]G
764 CCCGAG
BglII 990 AGATCT A^GATCT
3787 AGATCT
BsgI 3138 GTGCAG GTGCAG(16/14)
3848 GTGCAG
BsmBI 41 CGTCTC CGTCTC(1/5)
622 GAGACG
BsoBI 70 CCCGGG C^[CT]CG[GA]G
764 CCCGAG
BsrBI 164 CCGCTC CCGCTC(-3/-3)
1486 CCGCTC
BssSI 243 CACGAG CACGAG(-5/-1)
200 CTCGTG
BstEII 1196 GGTAACC G^GT[ACGT]ACC
3394 GGTTACC
BstPI 1196 GGTAACC G^GT[ACGT]ACC
3394 GGTTACC
Bsu36I 1429 CCTGAGG CC^T[ACGT]AGG
4178 CCTAAGG
DraI 2582 TTTAAA TTT^AAA
3319 TTTAAA
DraIII 1147 CACCATGTG CAC[ACGT][ACGT][ACGT]^GTG
1938 CACCTAGTG
EagI 305 CGGCCG C^GGCCG
913 CGGCCG
EarI 770 CTCTTC CTCTTC(1/4)
229 GAAGAG
EclXI 305 CGGCCG C^GGCCG
913 CGGCCG
Eco52I 305 CGGCCG C^GGCCG
913 CGGCCG
Eco81I 1429 CCTGAGG CC^T[ACGT]AGG
4178 CCTAAGG
EcoO65I 1196 GGTAACC G^GT[ACGT]ACC
3394 GGTTACC
HindIII 806 AAGCTT A^AGCTT
3324 AAGCTT
Ksp632I 770 CTCTTC CTCTTC(1/4)
229 GAAGAG
KspI 428 CCGCGG CCGC^GG
477 CCGCGG
SacII 428 CCGCGG CCGC^GG
477 CCGCGG
SexAI 1374 ACCTGGT A^CC[AT]GGT
3130 ACCTGGT
SpeI 3333 ACTAGT A^CTAGT
3703 ACTAGT
SspI 2984 AATATT AAT^ATT
4372 AATATT
StuI 1609 AGGCCT AGG^CCT
2768 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 119 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaII
BalI BanI BanII BbeI BbsI
BbvI BcnI BfaI BlpI BmyI
BpmI Bpu1102I BpuAI BsaBI BsaHI
BsaJI BseRI BsiEI BsiHKAI BsiYI
BslI BsmAI BsmFI BsmI Bsp1286I
BspMI BsrDI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NarI NciI NdeII
NlaIII NlaIV NspI PleI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XcmI XhoII