################################## # NO CUT ENZYMES ( 85 enzymes) # ################################## AatII AccII AclI AcyI AflII AgeI AhdI Aor51HI AscI AspEI AspI AviII AvrII BbeI BbrPI BfrI BlnI BsaAI BsaHI BsaI BsiEI BsiWI BsmBI BsmI BspDI BsrBI BssHII BssSI BstBI BstUI BstXI ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FseI FspI HgaI Hin1I KasI KspI MluI MvnI NarI NdeI NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI Psp1406I PvuI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### Acc65I 2409 GGTACC G^GTACC AccI 1425 GTATAC GT^[AC][GT]AC AccIII 5 TCCGGA T^CCGGA AflIII 3650 ACATGT A^C[GA][CT]GT Asp700I 1907 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2409 GGTACC G^GTACC BamHI 4367 GGATCC G^GATCC BcgI 1222 CGAAGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 489 GGATAC GTATCC(6/5) BclI 3802 TGATCA T^GATCA BlpI 689 GCTGAGC GC^T[ACGT]AGC Bpu1102I 689 GCTGAGC GC^T[ACGT]AGC BsaBI 392 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 5 TCCGGA [AT]^CCGG[AT] BseAI 5 TCCGGA T^CCGGA BspEI 5 TCCGGA T^CCGGA BspLU11I 3650 ACATGT A^CATGT BspMI 1545 ACCTGC ACCTGC(4/8) BsrFI 1053 GCCGGC [GA]^CCGG[CT] Bst1107I 1425 GTATAC GTA^TAC BstZ17I 1425 GTATAC GTA^TAC CelII 689 GCTGAGC GC^T[ACGT]AGC Cfr10I 1053 GCCGGC [GA]^CCGG[CT] DrdI 2888 GACATTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC FbaI 3802 TGATCA T^GATCA HaeII 704 AGCGCC [GA]GCGC^[CT] HindIII 2639 AAGCTT A^AGCTT HpaI 4488 GTTAAC GTT^AAC KpnI 2409 GGTACC GGTAC^C MamI 392 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MfeI 4640 CAATTG C^AATTG MroI 5 TCCGGA T^CCGGA MunI 4640 CAATTG C^AATTG NaeI 1053 GCCGGC GCC^GGC NgoMIV 1053 GCCGGC G^CCGGC PaeR7I 1237 CTCGAG C^TCGAG SacI 3518 GAGCTC GAGCT^C SmaI 3396 CCCGGG CCC^GGG SphI 3023 GCATGC GCATG^C XcmI 2193 CCATCGTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XhoI 1237 CTCGAG C^TCGAG XmaCI 3396 CCCGGG C^CCGGG XmaI 3396 CCCGGG C^CCGGG XmnI 1907 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 22 enzymes) # ################################### Alw44I 935 GTGCAC G^TGCAC 2111 GTGCAC AocI 1124 CCTAAGG CC^T[ACGT]AGG 2236 CCTTAGG ApaI 2845 GGGCCC GGGCC^C 3440 GGGCCC ApaLI 935 GTGCAC G^TGCAC 2111 GTGCAC BbvCI 2516 GCTGAGG CCTCAGC(-5/-2) 3578 GCTGAGG BglI 2465 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 4519 GCCAGGGAGGC BglII 1843 AGATCT A^GATCT 4320 AGATCT BspHI 1575 TCATGA T^CATGA 1771 TCATGA BstAPI 1057 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2921 GCATGTGCTGC BstEII 943 GGTCACC G^GT[ACGT]ACC 2896 GGTCACC BstPI 943 GGTCACC G^GT[ACGT]ACC 2896 GGTCACC Bsu36I 1124 CCTAAGG CC^T[ACGT]AGG 2236 CCTTAGG Eco81I 1124 CCTAAGG CC^T[ACGT]AGG 2236 CCTTAGG EcoO65I 943 GGTCACC G^GT[ACGT]ACC 2896 GGTCACC FauI 3843 CCCGC CCCGC(4/6) 3791 GCGGG HincII 1047 GTCAAC GT[CT]^[GA]AC 4488 GTTAAC HindII 1047 GTCAAC GT[CT]^[GA]AC 4488 GTTAAC MaeII 443 ACGT A^CGT 3363 ACGT PpuMI 951 AGGACCT [GA]G^G[AT]CC[CT] 4555 AGGTCCT RcaI 1575 TCATGA T^CATGA 1771 TCATGA TaqI 1238 TCGA T^CGA 1529 TCGA TthHB8I 1238 TCGA T^CGA 1529 TCGA ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII