##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AclI AcyI AflII
AgeI AhdI Aor51HI AscI AspEI
AspI AviII AvrII BbeI BbrPI
BfrI BlnI BsaAI BsaHI BsaI
BsiEI BsiWI BsmBI BsmI BspDI
BsrBI BssHII BssSI BstBI BstUI
BstXI ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoRI EcoRV EcoT22I FseI FspI
HgaI Hin1I KasI KspI MluI
MvnI NarI NdeI NheI NotI
NruI NsiI NspV PacI PflFI
PinAI PmaCI PmeI PmlI PshAI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
Sse8387I SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
Acc65I 2409 GGTACC G^GTACC
AccI 1425 GTATAC GT^[AC][GT]AC
AccIII 5 TCCGGA T^CCGGA
AflIII 3650 ACATGT A^C[GA][CT]GT
Asp700I 1907 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2409 GGTACC G^GTACC
BamHI 4367 GGATCC G^GATCC
BcgI 1222 CGAAGACTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 489 GGATAC GTATCC(6/5)
BclI 3802 TGATCA T^GATCA
BlpI 689 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 689 GCTGAGC GC^T[ACGT]AGC
BsaBI 392 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 5 TCCGGA [AT]^CCGG[AT]
BseAI 5 TCCGGA T^CCGGA
BspEI 5 TCCGGA T^CCGGA
BspLU11I 3650 ACATGT A^CATGT
BspMI 1545 ACCTGC ACCTGC(4/8)
BsrFI 1053 GCCGGC [GA]^CCGG[CT]
Bst1107I 1425 GTATAC GTA^TAC
BstZ17I 1425 GTATAC GTA^TAC
CelII 689 GCTGAGC GC^T[ACGT]AGC
Cfr10I 1053 GCCGGC [GA]^CCGG[CT]
DrdI 2888 GACATTAAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FbaI 3802 TGATCA T^GATCA
HaeII 704 AGCGCC [GA]GCGC^[CT]
HindIII 2639 AAGCTT A^AGCTT
HpaI 4488 GTTAAC GTT^AAC
KpnI 2409 GGTACC GGTAC^C
MamI 392 GATATTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 4640 CAATTG C^AATTG
MroI 5 TCCGGA T^CCGGA
MunI 4640 CAATTG C^AATTG
NaeI 1053 GCCGGC GCC^GGC
NgoMIV 1053 GCCGGC G^CCGGC
PaeR7I 1237 CTCGAG C^TCGAG
SacI 3518 GAGCTC GAGCT^C
SmaI 3396 CCCGGG CCC^GGG
SphI 3023 GCATGC GCATG^C
XcmI 2193 CCATCGTGACTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1237 CTCGAG C^TCGAG
XmaCI 3396 CCCGGG C^CCGGG
XmaI 3396 CCCGGG C^CCGGG
XmnI 1907 GAACAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
Alw44I 935 GTGCAC G^TGCAC
2111 GTGCAC
AocI 1124 CCTAAGG CC^T[ACGT]AGG
2236 CCTTAGG
ApaI 2845 GGGCCC GGGCC^C
3440 GGGCCC
ApaLI 935 GTGCAC G^TGCAC
2111 GTGCAC
BbvCI 2516 GCTGAGG CCTCAGC(-5/-2)
3578 GCTGAGG
BglI 2465 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
4519 GCCAGGGAGGC
BglII 1843 AGATCT A^GATCT
4320 AGATCT
BspHI 1575 TCATGA T^CATGA
1771 TCATGA
BstAPI 1057 GCAGTTTCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2921 GCATGTGCTGC
BstEII 943 GGTCACC G^GT[ACGT]ACC
2896 GGTCACC
BstPI 943 GGTCACC G^GT[ACGT]ACC
2896 GGTCACC
Bsu36I 1124 CCTAAGG CC^T[ACGT]AGG
2236 CCTTAGG
Eco81I 1124 CCTAAGG CC^T[ACGT]AGG
2236 CCTTAGG
EcoO65I 943 GGTCACC G^GT[ACGT]ACC
2896 GGTCACC
FauI 3843 CCCGC CCCGC(4/6)
3791 GCGGG
HincII 1047 GTCAAC GT[CT]^[GA]AC
4488 GTTAAC
HindII 1047 GTCAAC GT[CT]^[GA]AC
4488 GTTAAC
MaeII 443 ACGT A^CGT
3363 ACGT
PpuMI 951 AGGACCT [GA]G^G[AT]CC[CT]
4555 AGGTCCT
RcaI 1575 TCATGA T^CATGA
1771 TCATGA
TaqI 1238 TCGA T^CGA
1529 TCGA
TthHB8I 1238 TCGA T^CGA
1529 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AseI AsnI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I Bsp1407I BsrDI BsrGI
BsrI BssKI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV NspI PflMI
PleI PshBI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI SspBI StuI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII