##################################
# NO CUT ENZYMES (   68 enzymes) #
##################################

AatII       Acc65I      AccIII      AclI        AgeI        
AhdI        Aor51HI     AscI        Asp718I     AspEI       
AspI        AviII       BcgI        BciVI       BsaWI       
BseAI       BsiWI       BsmBI       BspDI       BspEI       
BspHI       BspLU11I    BsrBI       BssHII      Bst1107I    
BstZ17I     ClaI        CpoI        DrdI        Eam1105I    
Eco47III    FseI        FspI        KpnI        KspI        
MfeI        MluI        MroI        MunI        NheI        
NotI        NruI        PaeR7I      PflFI       PflMI       
PinAI       PmeI        Psp1406I    PvuI        RcaI        
RsrII       SacI        SacII       SbfI        ScaI        
SfiI        SgrAI       SmaI        SnaBI       SpeI        
SphI        Sse8387I    SwaI        Tth111I     Van91I      
XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   40 enzymes) #
###################################

AccII         476  CGCG                 CG^CG
AflII         828  CTTAAG               C^TTAAG
AflIII       3341  ACGTGT               A^C[GA][CT]GT
Alw44I       1520  GTGCAC               G^TGCAC
ApaI          593  GGGCCC               GGGCC^C
ApaLI        1520  GTGCAC               G^TGCAC
BbrPI        2257  CACGTG               CAC^GTG
BbvCI         455  CCTCAGC              CCTCAGC(-5/-2)
BclI         1573  TGATCA               T^GATCA
BfrI          828  CTTAAG               C^TTAAG
BlpI         2467  GCTAAGC              GC^T[ACGT]AGC
Bpu1102I     2467  GCTAAGC              GC^T[ACGT]AGC
BsaAI        2257  CACGTG               [CT]AC^GT[GA]
BsiEI         895  CGGCCG               CG[GA][CT]^CG
BsmI         3993  GCATTC               GAATGC(1/-1)
BsrFI         112  GCCGGC               [GA]^CCGG[CT]
BstBI        1357  TTCGAA               TT^CGAA
BstUI         476  CGCG                 CG^CG
CelII        2467  GCTAAGC              GC^T[ACGT]AGC
Cfr10I        112  GCCGGC               [GA]^CCGG[CT]
EagI          895  CGGCCG               C^GGCCG
EclXI         895  CGGCCG               C^GGCCG
Eco52I        895  CGGCCG               C^GGCCG
EcoRI        2693  GAATTC               G^AATTC
EcoRV        3280  GATATC               GAT^ATC
EcoT22I      2364  ATGCAT               ATGCA^T
FbaI         1573  TGATCA               T^GATCA
HpaI         4174  GTTAAC               GTT^AAC
MvnI          476  CGCG                 CG^CG
NaeI          112  GCCGGC               GCC^GGC
NgoMIV        112  GCCGGC               G^CCGGC
NsiI         2364  ATGCAT               ATGCA^T
NspI         2353  ACATGC               [GA]CATG^[CT]
NspV         1357  TTCGAA               TT^CGAA
PmaCI        2257  CACGTG               CAC^GTG
PmlI         2257  CACGTG               CAC^GTG
SalI         2856  GTCGAC               G^TCGAC
SapI         1074  GAAGAGC              GCTCTTC(1/4)
SfuI         1357  TTCGAA               TT^CGAA
XbaI         2647  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   32 enzymes) #
###################################

AocI         1148  CCTCAGG              CC^T[ACGT]AGG
             2192  CCTGAGG             
Asp700I      2815  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3776  GAATTGTTTC          
AvaI          251  CTCGGG               C^[CT]CG[GA]G
              549  CCCGAG              
AvrII          11  CCTAGG               C^CTAGG
               19  CCTAGG              
BanII         248  GGGCTC               G[GA]GC[CT]^C
              593  GGGCCC              
BbeI           15  GGCGCC               GGCGC^C
              224  GGCGCC              
BcnI           71  CCCGG                CC^[GC]GG
             1322  CCGGG               
BglI          116  GCCTGGGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             2199  GCCAGGAGGGC         
BlnI           11  CCTAGG               C^CTAGG
               19  CCTAGG              
BsoBI         251  CTCGGG               C^[CT]CG[GA]G
              549  CCCGAG              
BsrDI        1980  CATTGC               GCAATG(2/0)
             3520  CATTGC              
BssSI        4104  CACGAG               CACGAG(-5/-1)
             3355  CTCGTG              
BstAPI       1103  GCAGAAGCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
             3703  GCAGAGCCTGC         
BstEII       2087  GGTCACC              G^GT[ACGT]ACC
             2672  GGTGACC             
BstPI        2087  GGTCACC              G^GT[ACGT]ACC
             2672  GGTGACC             
Bsu36I       1148  CCTCAGG              CC^T[ACGT]AGG
             2192  CCTGAGG             
DraIII        126  CACAGTGTG            CAC[ACGT][ACGT][ACGT]^GTG
             2257  CACGTGGTG           
Eco81I       1148  CCTCAGG              CC^T[ACGT]AGG
             2192  CCTGAGG             
EcoO65I      2087  GGTCACC              G^GT[ACGT]ACC
             2672  GGTGACC             
HindIII      2817  AAGCTT               A^AGCTT
             4219  AAGCTT              
KasI           15  GGCGCC               G^GCGCC
              224  GGCGCC              
NarI           15  GGCGCC               GG^CGCC
              224  GGCGCC              
NciI           71  CCCGG                CC^[GC]GG
             1322  CCGGG               
NcoI          214  CCATGG               C^CATGG
             3904  CCATGG              
NdeI         3253  CATATG               CA^TATG
             3819  CATATG              
PacI         2635  TTAATTAA             TTAAT^TAA
             4301  TTAATTAA            
PpuMI        1115  GGGACCT              [GA]G^G[AT]CC[CT]
             2130  GGGACCT             
PshAI        1843  GACAGGTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
             2444  GACAAGTGTC          
SexAI        2083  ACCTGGT              A^CC[AT]GGT
             2321  ACCTGGT             
SfoI           15  GGCGCC               GGC^GCC
              224  GGCGCC              
SmlI          828  CTTAAG               C^T[CT][GA]AG
             2038  CTCAAG              
XmnI         2815  GAAAGCTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
             3776  GAATTGTTTC          


#########################################
# 3 OR MORE CUT ENZYMES (  118 enzymes) #
#########################################

AccI        AciI        AcsI        AcyI        AfaI        
AluI        AlwI        AlwNI       ApoI        AseI        
AsnI        AspHI       AvaII       BalI        BamHI       
BanI        BbsI        BbvI        BfaI        BglII       
BmyI        BpmI        BpuAI       BsaBI       BsaHI       
BsaI        BsaJI       BseRI       BsgI        BsiHKAI     
BsiYI       BslI        BsmAI       BsmFI       Bsp1286I    
Bsp1407I    BspMI       BsrGI       BsrI        BssKI       
BstF5I      BstNI       BstXI       BstYI       Cac8I       
CfoI        Cfr13I      DdeI        DpnII       DraI        
DraII       DsaI        EaeI        EarI        EcoNI       
EcoO109I    EcoRII      EcoT14I     FauI        Fnu4HI      
FokI        HaeII       HaeIII      HapII       HgaI        
HhaI        Hin1I       HinP1I      HincII      HindII      
HinfI       HpaII       HphI        ItaI        Ksp632I     
MaeI        MaeII       MaeIII      MamI        MboI        
MboII       MflI        MluNI       MnlI        MscI        
MseI        MslI        MspA1I      MspI        MvaI        
MwoI        NdeII       NlaIII      NlaIV       PleI        
PshBI       PstI        PvuII       RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SspBI       
SspI        StuI        StyI        TaqI        TfiI        
Tru9I       TseI        Tsp45I      Tsp509I     TspRI       
TthHB8I     XcmI        XhoII