################################## # NO CUT ENZYMES ( 68 enzymes) # ################################## AatII Acc65I AccIII AclI AgeI AhdI Aor51HI AscI Asp718I AspEI AspI AviII BcgI BciVI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BssHII Bst1107I BstZ17I ClaI CpoI DrdI Eam1105I Eco47III FseI FspI KpnI KspI MfeI MluI MroI MunI NheI NotI NruI PaeR7I PflFI PflMI PinAI PmeI Psp1406I PvuI RcaI RsrII SacI SacII SbfI ScaI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SwaI Tth111I Van91I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 40 enzymes) # ################################### AccII 476 CGCG CG^CG AflII 828 CTTAAG C^TTAAG AflIII 3341 ACGTGT A^C[GA][CT]GT Alw44I 1520 GTGCAC G^TGCAC ApaI 593 GGGCCC GGGCC^C ApaLI 1520 GTGCAC G^TGCAC BbrPI 2257 CACGTG CAC^GTG BbvCI 455 CCTCAGC CCTCAGC(-5/-2) BclI 1573 TGATCA T^GATCA BfrI 828 CTTAAG C^TTAAG BlpI 2467 GCTAAGC GC^T[ACGT]AGC Bpu1102I 2467 GCTAAGC GC^T[ACGT]AGC BsaAI 2257 CACGTG [CT]AC^GT[GA] BsiEI 895 CGGCCG CG[GA][CT]^CG BsmI 3993 GCATTC GAATGC(1/-1) BsrFI 112 GCCGGC [GA]^CCGG[CT] BstBI 1357 TTCGAA TT^CGAA BstUI 476 CGCG CG^CG CelII 2467 GCTAAGC GC^T[ACGT]AGC Cfr10I 112 GCCGGC [GA]^CCGG[CT] EagI 895 CGGCCG C^GGCCG EclXI 895 CGGCCG C^GGCCG Eco52I 895 CGGCCG C^GGCCG EcoRI 2693 GAATTC G^AATTC EcoRV 3280 GATATC GAT^ATC EcoT22I 2364 ATGCAT ATGCA^T FbaI 1573 TGATCA T^GATCA HpaI 4174 GTTAAC GTT^AAC MvnI 476 CGCG CG^CG NaeI 112 GCCGGC GCC^GGC NgoMIV 112 GCCGGC G^CCGGC NsiI 2364 ATGCAT ATGCA^T NspI 2353 ACATGC [GA]CATG^[CT] NspV 1357 TTCGAA TT^CGAA PmaCI 2257 CACGTG CAC^GTG PmlI 2257 CACGTG CAC^GTG SalI 2856 GTCGAC G^TCGAC SapI 1074 GAAGAGC GCTCTTC(1/4) SfuI 1357 TTCGAA TT^CGAA XbaI 2647 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AocI 1148 CCTCAGG CC^T[ACGT]AGG 2192 CCTGAGG Asp700I 2815 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3776 GAATTGTTTC AvaI 251 CTCGGG C^[CT]CG[GA]G 549 CCCGAG AvrII 11 CCTAGG C^CTAGG 19 CCTAGG BanII 248 GGGCTC G[GA]GC[CT]^C 593 GGGCCC BbeI 15 GGCGCC GGCGC^C 224 GGCGCC BcnI 71 CCCGG CC^[GC]GG 1322 CCGGG BglI 116 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 2199 GCCAGGAGGGC BlnI 11 CCTAGG C^CTAGG 19 CCTAGG BsoBI 251 CTCGGG C^[CT]CG[GA]G 549 CCCGAG BsrDI 1980 CATTGC GCAATG(2/0) 3520 CATTGC BssSI 4104 CACGAG CACGAG(-5/-1) 3355 CTCGTG BstAPI 1103 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 3703 GCAGAGCCTGC BstEII 2087 GGTCACC G^GT[ACGT]ACC 2672 GGTGACC BstPI 2087 GGTCACC G^GT[ACGT]ACC 2672 GGTGACC Bsu36I 1148 CCTCAGG CC^T[ACGT]AGG 2192 CCTGAGG DraIII 126 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG 2257 CACGTGGTG Eco81I 1148 CCTCAGG CC^T[ACGT]AGG 2192 CCTGAGG EcoO65I 2087 GGTCACC G^GT[ACGT]ACC 2672 GGTGACC HindIII 2817 AAGCTT A^AGCTT 4219 AAGCTT KasI 15 GGCGCC G^GCGCC 224 GGCGCC NarI 15 GGCGCC GG^CGCC 224 GGCGCC NciI 71 CCCGG CC^[GC]GG 1322 CCGGG NcoI 214 CCATGG C^CATGG 3904 CCATGG NdeI 3253 CATATG CA^TATG 3819 CATATG PacI 2635 TTAATTAA TTAAT^TAA 4301 TTAATTAA PpuMI 1115 GGGACCT [GA]G^G[AT]CC[CT] 2130 GGGACCT PshAI 1843 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2444 GACAAGTGTC SexAI 2083 ACCTGGT A^CC[AT]GGT 2321 ACCTGGT SfoI 15 GGCGCC GGC^GCC 224 GGCGCC SmlI 828 CTTAAG C^T[CT][GA]AG 2038 CTCAAG XmnI 2815 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 3776 GAATTGTTTC ######################################### # 3 OR MORE CUT ENZYMES ( 118 enzymes) # ######################################### AccI AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AseI AsnI AspHI AvaII BalI BamHI BanI BbsI BbvI BfaI BglII BmyI BpmI BpuAI BsaBI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrGI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MamI MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PleI PshBI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI SspI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII