##################################
# NO CUT ENZYMES ( 68 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
AhdI Aor51HI AscI Asp718I AspEI
AspI AviII BcgI BciVI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspHI BspLU11I BsrBI BssHII Bst1107I
BstZ17I ClaI CpoI DrdI Eam1105I
Eco47III FseI FspI KpnI KspI
MfeI MluI MroI MunI NheI
NotI NruI PaeR7I PflFI PflMI
PinAI PmeI Psp1406I PvuI RcaI
RsrII SacI SacII SbfI ScaI
SfiI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SwaI Tth111I Van91I
XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 40 enzymes) #
###################################
AccII 476 CGCG CG^CG
AflII 828 CTTAAG C^TTAAG
AflIII 3341 ACGTGT A^C[GA][CT]GT
Alw44I 1520 GTGCAC G^TGCAC
ApaI 593 GGGCCC GGGCC^C
ApaLI 1520 GTGCAC G^TGCAC
BbrPI 2257 CACGTG CAC^GTG
BbvCI 455 CCTCAGC CCTCAGC(-5/-2)
BclI 1573 TGATCA T^GATCA
BfrI 828 CTTAAG C^TTAAG
BlpI 2467 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 2467 GCTAAGC GC^T[ACGT]AGC
BsaAI 2257 CACGTG [CT]AC^GT[GA]
BsiEI 895 CGGCCG CG[GA][CT]^CG
BsmI 3993 GCATTC GAATGC(1/-1)
BsrFI 112 GCCGGC [GA]^CCGG[CT]
BstBI 1357 TTCGAA TT^CGAA
BstUI 476 CGCG CG^CG
CelII 2467 GCTAAGC GC^T[ACGT]AGC
Cfr10I 112 GCCGGC [GA]^CCGG[CT]
EagI 895 CGGCCG C^GGCCG
EclXI 895 CGGCCG C^GGCCG
Eco52I 895 CGGCCG C^GGCCG
EcoRI 2693 GAATTC G^AATTC
EcoRV 3280 GATATC GAT^ATC
EcoT22I 2364 ATGCAT ATGCA^T
FbaI 1573 TGATCA T^GATCA
HpaI 4174 GTTAAC GTT^AAC
MvnI 476 CGCG CG^CG
NaeI 112 GCCGGC GCC^GGC
NgoMIV 112 GCCGGC G^CCGGC
NsiI 2364 ATGCAT ATGCA^T
NspI 2353 ACATGC [GA]CATG^[CT]
NspV 1357 TTCGAA TT^CGAA
PmaCI 2257 CACGTG CAC^GTG
PmlI 2257 CACGTG CAC^GTG
SalI 2856 GTCGAC G^TCGAC
SapI 1074 GAAGAGC GCTCTTC(1/4)
SfuI 1357 TTCGAA TT^CGAA
XbaI 2647 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AocI 1148 CCTCAGG CC^T[ACGT]AGG
2192 CCTGAGG
Asp700I 2815 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3776 GAATTGTTTC
AvaI 251 CTCGGG C^[CT]CG[GA]G
549 CCCGAG
AvrII 11 CCTAGG C^CTAGG
19 CCTAGG
BanII 248 GGGCTC G[GA]GC[CT]^C
593 GGGCCC
BbeI 15 GGCGCC GGCGC^C
224 GGCGCC
BcnI 71 CCCGG CC^[GC]GG
1322 CCGGG
BglI 116 GCCTGGGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2199 GCCAGGAGGGC
BlnI 11 CCTAGG C^CTAGG
19 CCTAGG
BsoBI 251 CTCGGG C^[CT]CG[GA]G
549 CCCGAG
BsrDI 1980 CATTGC GCAATG(2/0)
3520 CATTGC
BssSI 4104 CACGAG CACGAG(-5/-1)
3355 CTCGTG
BstAPI 1103 GCAGAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3703 GCAGAGCCTGC
BstEII 2087 GGTCACC G^GT[ACGT]ACC
2672 GGTGACC
BstPI 2087 GGTCACC G^GT[ACGT]ACC
2672 GGTGACC
Bsu36I 1148 CCTCAGG CC^T[ACGT]AGG
2192 CCTGAGG
DraIII 126 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
2257 CACGTGGTG
Eco81I 1148 CCTCAGG CC^T[ACGT]AGG
2192 CCTGAGG
EcoO65I 2087 GGTCACC G^GT[ACGT]ACC
2672 GGTGACC
HindIII 2817 AAGCTT A^AGCTT
4219 AAGCTT
KasI 15 GGCGCC G^GCGCC
224 GGCGCC
NarI 15 GGCGCC GG^CGCC
224 GGCGCC
NciI 71 CCCGG CC^[GC]GG
1322 CCGGG
NcoI 214 CCATGG C^CATGG
3904 CCATGG
NdeI 3253 CATATG CA^TATG
3819 CATATG
PacI 2635 TTAATTAA TTAAT^TAA
4301 TTAATTAA
PpuMI 1115 GGGACCT [GA]G^G[AT]CC[CT]
2130 GGGACCT
PshAI 1843 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2444 GACAAGTGTC
SexAI 2083 ACCTGGT A^CC[AT]GGT
2321 ACCTGGT
SfoI 15 GGCGCC GGC^GCC
224 GGCGCC
SmlI 828 CTTAAG C^T[CT][GA]AG
2038 CTCAAG
XmnI 2815 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
3776 GAATTGTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 118 enzymes) #
#########################################
AccI AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AseI
AsnI AspHI AvaII BalI BamHI
BanI BbsI BbvI BfaI BglII
BmyI BpmI BpuAI BsaBI BsaHI
BsaI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
Bsp1407I BspMI BsrGI BsrI BssKI
BstF5I BstNI BstXI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DsaI EaeI EarI EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MwoI NdeII NlaIII NlaIV PleI
PshBI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspBI
SspI StuI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII