################################## # NO CUT ENZYMES ( 66 enzymes) # ################################## AatII Acc65I AflII AgeI Aor51HI ApaI AscI Asp718I AviII AvrII BbeI BbrPI BciVI BfrI BglI BglII BlnI BsiWI BspDI BspHI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI Eco47III EcoO65I EcoRV FspI HindIII KasI KpnI MfeI MluI MunI NarI NheI NotI NruI NspV PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PvuI RcaI RsrII SalI SbfI SexAI SfiI SfoI SfuI SgrAI SmlI Sse8387I SwaI XcmI XhoI ################################### # ONE CUT ENZYMES ( 51 enzymes) # ################################### AccI 3655 GTCTAC GT^[AC][GT]AC AccIII 484 TCCGGA T^CCGGA AclI 2534 AACGTT AA^CGTT AcyI 4897 GACGCC G[GA]^CG[CT]C AhdI 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 4725 GTGCAC G^TGCAC ApaLI 4725 GTGCAC G^TGCAC AseI 4256 ATTAAT AT^TAAT AsnI 4256 ATTAAT AT^TAAT Asp700I 3091 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AspEI 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC BcgI 76 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2433 TGATCA T^GATCA BlpI 2852 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2852 GCTCAGC GC^T[ACGT]AGC BsaHI 4897 GACGCC G[GA]^CG[CT]C BsaI 3232 GAGACC GGTCTC(1/5) BseAI 484 TCCGGA T^CCGGA BseRI 1914 GAGGAG GAGGAG(10/8) BsmBI 596 GAGACG CGTCTC(1/5) BsmI 144 GCATTC GAATGC(1/-1) BspEI 484 TCCGGA T^CCGGA BsrDI 2236 GCAATG GCAATG(2/0) CelII 2852 GCTCAGC GC^T[ACGT]AGC DraI 3623 TTTAAA TTT^AAA Eam1105I 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 2249 GAATTC G^AATTC EcoT22I 3814 ATGCAT ATGCA^T FbaI 2433 TGATCA T^GATCA FseI 1016 GGCCGGCC GGCCGG^CC Hin1I 4897 GACGCC G[GA]^CG[CT]C HincII 2576 GTTAAC GT[CT]^[GA]AC HindII 2576 GTTAAC GT[CT]^[GA]AC HpaI 2576 GTTAAC GTT^AAC MroI 484 TCCGGA T^CCGGA NaeI 1017 GCCGGC GCC^GGC NdeI 3383 CATATG CA^TATG NgoMIV 1017 GCCGGC G^CCGGC NsiI 3814 ATGCAT ATGCA^T PflFI 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC PshBI 4256 ATTAAT AT^TAAT Psp1406I 2534 AACGTT AA^CGTT ScaI 1918 AGTACT AGT^ACT SnaBI 3830 TACGTA TAC^GTA SphI 4827 GCATGC GCATG^C SspI 1934 AATATT AAT^ATT StuI 1332 AGGCCT AGG^CCT Tth111I 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC XbaI 3179 TCTAGA T^CTAGA XmnI 3091 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AocI 4095 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG BanI 2080 GGCACC G^G[CT][GA]CC 4355 GGCACC BsaAI 1184 CACGTA [CT]AC^GT[GA] 3830 TACGTA BsaBI 668 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4234 GATTTCTATC BsaWI 484 TCCGGA [AT]^CCGG[AT] 1314 ACCGGA BssSI 3865 CACGAG CACGAG(-5/-1) 1509 CTCGTG BstXI 1342 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2639 CCACACAGATGG Bsu36I 4095 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG EagI 114 CGGCCG C^GGCCG 1693 CGGCCG EclXI 114 CGGCCG C^GGCCG 1693 CGGCCG Eco52I 114 CGGCCG C^GGCCG 1693 CGGCCG Eco81I 4095 CCTGAGG CC^T[ACGT]AGG 4752 CCTGAGG KspI 47 CCGCGG CCGC^GG 1053 CCGCGG MamI 668 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 4234 GATTTCTATC NcoI 4123 CCATGG C^CATGG 4902 CCATGG PstI 837 CTGCAG CTGCA^G 4884 CTGCAG SacI 909 GAGCTC GAGCT^C 1197 GAGCTC SacII 47 CCGCGG CCGC^GG 1053 CCGCGG SapI 1160 GCTCTTC GCTCTTC(1/4) 1199 GCTCTTC SmaI 95 CCCGGG CCC^GGG 792 CCCGGG SpeI 1250 ACTAGT A^CTAGT 4523 ACTAGT TaqI 56 TCGA T^CGA 1701 TCGA TthHB8I 56 TCGA T^CGA 1701 TCGA XmaCI 95 CCCGGG C^CCGGG 792 CCCGGG XmaI 95 CCCGGG C^CCGGG 792 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 116 enzymes) # ######################################### AccII AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BamHI BanII BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaJI BsgI BsiEI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I BspMI BsrBI BsrFI BsrGI BsrI BssHII BssKI BstAPI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspBI StyI TfiI Tru9I TseI Tsp45I Tsp509I TspRI Van91I XhoII