##################################
# NO CUT ENZYMES ( 66 enzymes) #
##################################
AatII Acc65I AflII AgeI Aor51HI
ApaI AscI Asp718I AviII AvrII
BbeI BbrPI BciVI BfrI BglI
BglII BlnI BsiWI BspDI BspHI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI Eco47III
EcoO65I EcoRV FspI HindIII KasI
KpnI MfeI MluI MunI NarI
NheI NotI NruI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshAI PvuI RcaI RsrII SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmlI Sse8387I SwaI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AccI 3655 GTCTAC GT^[AC][GT]AC
AccIII 484 TCCGGA T^CCGGA
AclI 2534 AACGTT AA^CGTT
AcyI 4897 GACGCC G[GA]^CG[CT]C
AhdI 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4725 GTGCAC G^TGCAC
ApaLI 4725 GTGCAC G^TGCAC
AseI 4256 ATTAAT AT^TAAT
AsnI 4256 ATTAAT AT^TAAT
Asp700I 3091 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcgI 76 GCAGCATCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2433 TGATCA T^GATCA
BlpI 2852 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2852 GCTCAGC GC^T[ACGT]AGC
BsaHI 4897 GACGCC G[GA]^CG[CT]C
BsaI 3232 GAGACC GGTCTC(1/5)
BseAI 484 TCCGGA T^CCGGA
BseRI 1914 GAGGAG GAGGAG(10/8)
BsmBI 596 GAGACG CGTCTC(1/5)
BsmI 144 GCATTC GAATGC(1/-1)
BspEI 484 TCCGGA T^CCGGA
BsrDI 2236 GCAATG GCAATG(2/0)
CelII 2852 GCTCAGC GC^T[ACGT]AGC
DraI 3623 TTTAAA TTT^AAA
Eam1105I 1614 GACAGGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2249 GAATTC G^AATTC
EcoT22I 3814 ATGCAT ATGCA^T
FbaI 2433 TGATCA T^GATCA
FseI 1016 GGCCGGCC GGCCGG^CC
Hin1I 4897 GACGCC G[GA]^CG[CT]C
HincII 2576 GTTAAC GT[CT]^[GA]AC
HindII 2576 GTTAAC GT[CT]^[GA]AC
HpaI 2576 GTTAAC GTT^AAC
MroI 484 TCCGGA T^CCGGA
NaeI 1017 GCCGGC GCC^GGC
NdeI 3383 CATATG CA^TATG
NgoMIV 1017 GCCGGC G^CCGGC
NsiI 3814 ATGCAT ATGCA^T
PflFI 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 4256 ATTAAT AT^TAAT
Psp1406I 2534 AACGTT AA^CGTT
ScaI 1918 AGTACT AGT^ACT
SnaBI 3830 TACGTA TAC^GTA
SphI 4827 GCATGC GCATG^C
SspI 1934 AATATT AAT^ATT
StuI 1332 AGGCCT AGG^CCT
Tth111I 349 GACCGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 3179 TCTAGA T^CTAGA
XmnI 3091 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AocI 4095 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
BanI 2080 GGCACC G^G[CT][GA]CC
4355 GGCACC
BsaAI 1184 CACGTA [CT]AC^GT[GA]
3830 TACGTA
BsaBI 668 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4234 GATTTCTATC
BsaWI 484 TCCGGA [AT]^CCGG[AT]
1314 ACCGGA
BssSI 3865 CACGAG CACGAG(-5/-1)
1509 CTCGTG
BstXI 1342 CCATTGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2639 CCACACAGATGG
Bsu36I 4095 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
EagI 114 CGGCCG C^GGCCG
1693 CGGCCG
EclXI 114 CGGCCG C^GGCCG
1693 CGGCCG
Eco52I 114 CGGCCG C^GGCCG
1693 CGGCCG
Eco81I 4095 CCTGAGG CC^T[ACGT]AGG
4752 CCTGAGG
KspI 47 CCGCGG CCGC^GG
1053 CCGCGG
MamI 668 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
4234 GATTTCTATC
NcoI 4123 CCATGG C^CATGG
4902 CCATGG
PstI 837 CTGCAG CTGCA^G
4884 CTGCAG
SacI 909 GAGCTC GAGCT^C
1197 GAGCTC
SacII 47 CCGCGG CCGC^GG
1053 CCGCGG
SapI 1160 GCTCTTC GCTCTTC(1/4)
1199 GCTCTTC
SmaI 95 CCCGGG CCC^GGG
792 CCCGGG
SpeI 1250 ACTAGT A^CTAGT
4523 ACTAGT
TaqI 56 TCGA T^CGA
1701 TCGA
TthHB8I 56 TCGA T^CGA
1701 TCGA
XmaCI 95 CCCGGG C^CCGGG
792 CCCGGG
XmaI 95 CCCGGG C^CCGGG
792 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaI AvaII BalI BamHI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrFI BsrGI BsrI
BssHII BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I
XhoII