##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII AclI AgeI AhdI Alw44I
Aor51HI ApaLI AscI AspEI AspI
AvaI AviII AvrII BamHI BbrPI
BcgI BlnI BsiWI BsoBI BspDI
BspHI BspLU11I BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I ClaI CpoI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI FspI MfeI
MluI MunI NcoI NdeI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI Psp1406I
RcaI RsrII SacI SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI SwaI Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
Acc65I 3560 GGTACC G^GTACC
AccI 2734 GTCTAC GT^[AC][GT]AC
AflII 876 CTTAAG C^TTAAG
AflIII 2336 ACGTGT A^C[GA][CT]GT
Asp700I 1510 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3560 GGTACC G^GTACC
BalI 2703 TGGCCA TGG^CCA
BbeI 168 GGCGCC GGCGC^C
BbvCI 1539 GCTGAGG CCTCAGC(-5/-2)
BciVI 2450 GGATAC GTATCC(6/5)
BclI 1303 TGATCA T^GATCA
BfrI 876 CTTAAG C^TTAAG
BglI 427 GCCAGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 2335 TACGTG [CT]AC^GT[GA]
BsaBI 902 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 207 CGATCG CG[GA][CT]^CG
BsmI 945 GCATTC GAATGC(1/-1)
BsrDI 886 GCAATG GCAATG(2/0)
BstAPI 1638 GCACCCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 2806 GGTGACC G^GT[ACGT]ACC
BstPI 2806 GGTGACC G^GT[ACGT]ACC
DraI 3570 TTTAAA TTT^AAA
DraIII 2287 CACTGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 1393 CCTTCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2806 GGTGACC G^GT[ACGT]ACC
EcoRV 3599 GATATC GAT^ATC
FbaI 1303 TGATCA T^GATCA
HpaI 2439 GTTAAC GTT^AAC
KasI 168 GGCGCC G^GCGCC
KpnI 3560 GGTACC GGTAC^C
KspI 1603 CCGCGG CCGC^GG
MamI 902 GATACTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 2703 TGGCCA TGG^CCA
MscI 2703 TGGCCA TGG^CCA
NaeI 26 GCCGGC GCC^GGC
NarI 168 GGCGCC GG^CGCC
NgoMIV 26 GCCGGC G^CCGGC
NheI 2938 GCTAGC G^CTAGC
PshAI 360 GACTTTAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 207 CGATCG CGAT^CG
SacII 1603 CCGCGG CCGC^GG
SapI 1461 GCTCTTC GCTCTTC(1/4)
SfoI 168 GGCGCC GGC^GCC
StuI 2223 AGGCCT AGG^CCT
TfiI 2567 GATTC G^A[AT]TC
XbaI 2358 TCTAGA T^CTAGA
XmnI 1510 GAACCCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccIII 58 TCCGGA T^CCGGA
301 TCCGGA
AcsI 1942 AAATTC [GA]^AATT[CT]
3074 AAATTC
AcyI 168 GGCGCC G[GA]^CG[CT]C
771 GGCGTC
AlwNI 1172 CAGCGCCTG CAG[ACGT][ACGT][ACGT]^CTG
3455 CAGCCACTG
ApaI 2547 GGGCCC GGGCC^C
3273 GGGCCC
ApoI 1942 AAATTC [GA]^AATT[CT]
3074 AAATTC
AseI 1690 ATTAAT AT^TAAT
3168 ATTAAT
AsnI 1690 ATTAAT AT^TAAT
3168 ATTAAT
BglII 1872 AGATCT A^GATCT
3529 AGATCT
BsaHI 168 GGCGCC G[GA]^CG[CT]C
771 GGCGTC
BseAI 58 TCCGGA T^CCGGA
301 TCCGGA
BseRI 2187 CTCCTC GAGGAG(10/8)
1542 GAGGAG
BsmBI 156 CGTCTC CGTCTC(1/5)
378 GAGACG
BspEI 58 TCCGGA T^CCGGA
301 TCCGGA
BsrFI 26 GCCGGC [GA]^CCGG[CT]
37 GCCGGT
BstXI 2096 CCAGTATTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3550 CCACCCAAGTGG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
37 GCCGGT
DsaI 1334 CCACGG C^C[GA][CT]GG
1603 CCGCGG
EaeI 1255 CGGCCA [CT]^GGCC[GA]
2703 TGGCCA
EcoT22I 711 ATGCAT ATGCA^T
1591 ATGCAT
Hin1I 168 GGCGCC G[GA]^CG[CT]C
771 GGCGTC
HindIII 1009 AAGCTT A^AGCTT
2026 AAGCTT
MroI 58 TCCGGA T^CCGGA
301 TCCGGA
MslI 1986 CATCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3551 CACCCAAGTG
NsiI 711 ATGCAT ATGCA^T
1591 ATGCAT
NspI 1658 GCATGT [GA]CATG^[CT]
2321 ACATGC
PshBI 1690 ATTAAT AT^TAAT
3168 ATTAAT
PstI 1414 CTGCAG CTGCA^G
2881 CTGCAG
PvuII 2933 CAGCTG CAG^CTG
3429 CAGCTG
SmlI 876 CTTAAG C^T[CT][GA]AG
3210 CTTGAG
TaqI 671 TCGA T^CGA
1006 TCGA
TthHB8I 671 TCGA T^CGA
1006 TCGA
XcmI 619 CCATGTCACCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1067 CCACGTCTGGAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AocI AspHI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BsaWI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrGI BsrI BssKI BstF5I
BstNI BstUI BstYI Bsu36I Cac8I
CelII CfoI Cfr13I DdeI DpnII
DraII EarI Eco81I EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV PflMI
PleI PpuMI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SspBI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
Van91I XhoII