################################## # NO CUT ENZYMES ( 53 enzymes) # ################################## AatII AccIII AclI AgeI AhdI Aor51HI AscI AspEI BcgI BclI BglI BsaBI BsaWI BseAI BsiWI Bsp1407I BspDI BspEI BsrGI BssHII BstBI BstEII BstPI ClaI CpoI DraIII Eam1105I Eco47III EcoO65I EcoRI FbaI FseI MamI MluI MroI NruI NspV PacI PinAI PmeI Psp1406I PvuI RsrII SacI SalI SbfI SfiI SfuI SgrAI SnaBI Sse8387I SspBI SwaI ################################### # ONE CUT ENZYMES ( 39 enzymes) # ################################### Acc65I 1728 GGTACC G^GTACC AflII 5027 CTTAAG C^TTAAG ApaI 344 GGGCCC GGGCC^C AseI 5162 ATTAAT AT^TAAT AsnI 5162 ATTAAT AT^TAAT Asp700I 2007 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1728 GGTACC G^GTACC BbrPI 3012 CACGTG CAC^GTG BfrI 5027 CTTAAG C^TTAAG BglII 5221 AGATCT A^GATCT BsaAI 3012 CACGTG [CT]AC^GT[GA] BsaI 5861 GGTCTC GGTCTC(1/5) BsgI 5754 CTGCAC GTGCAG(16/14) BsiEI 142 CGGCCG CG[GA][CT]^CG BspHI 2495 TCATGA T^CATGA BssSI 3852 CTCGTG CACGAG(-5/-1) Bst1107I 4190 GTATAC GTA^TAC BstZ17I 4190 GTATAC GTA^TAC EagI 142 CGGCCG C^GGCCG EclXI 142 CGGCCG C^GGCCG Eco52I 142 CGGCCG C^GGCCG EcoNI 215 CCTTGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoT22I 1011 ATGCAT ATGCA^T HindIII 3432 AAGCTT A^AGCTT KpnI 1728 GGTACC GGTAC^C KspI 152 CCGCGG CCGC^GG NcoI 910 CCATGG C^CATGG NdeI 653 CATATG CA^TATG NheI 99 GCTAGC G^CTAGC NotI 141 GCGGCCGC GC^GGCCGC NsiI 1011 ATGCAT ATGCA^T PmaCI 3012 CACGTG CAC^GTG PmlI 3012 CACGTG CAC^GTG PshBI 5162 ATTAAT AT^TAAT RcaI 2495 TCATGA T^CATGA SacII 152 CCGCGG CCGC^GG ScaI 1789 AGTACT AGT^ACT XcmI 4221 CCACTTTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 2007 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 35 enzymes) # ################################### AccI 4190 GTATAC GT^[AC][GT]AC 5941 GTCTAC AspI 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACCTGGTC AviII 218 TGCGCA TGC^GCA 5629 TGCGCA AvrII 5079 CCTAGG C^CTAGG 5497 CCTAGG BalI 4213 TGGCCA TGG^CCA 5701 TGGCCA BamHI 2105 GGATCC G^GATCC 5555 GGATCC BciVI 2859 GGATAC GTATCC(6/5) 5883 GTATCC BlnI 5079 CCTAGG C^CTAGG 5497 CCTAGG BlpI 2513 GCTTAGC GC^T[ACGT]AGC 5811 GCTGAGC Bpu1102I 2513 GCTTAGC GC^T[ACGT]AGC 5811 GCTGAGC BspLU11I 3712 ACATGT A^CATGT 4987 ACATGT BsrBI 16 CCGCTC CCGCTC(-3/-3) 115 GAGCGG BsrDI 1043 CATTGC GCAATG(2/0) 2506 CATTGC BsrFI 252 GCCGGC [GA]^CCGG[CT] 1940 GCCGGC CelII 2513 GCTTAGC GC^T[ACGT]AGC 5811 GCTGAGC Cfr10I 252 GCCGGC [GA]^CCGG[CT] 1940 GCCGGC DsaI 152 CCGCGG C^C[GA][CT]GG 910 CCATGG FspI 218 TGCGCA TGC^GCA 5629 TGCGCA HpaI 3454 GTTAAC GTT^AAC 4558 GTTAAC MluNI 4213 TGGCCA TGG^CCA 5701 TGGCCA MscI 4213 TGGCCA TGG^CCA 5701 TGGCCA NaeI 252 GCCGGC GCC^GGC 1940 GCCGGC NgoMIV 252 GCCGGC G^CCGGC 1940 GCCGGC PflFI 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACCTGGTC PflMI 649 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3244 CCAAAGATTGG PpuMI 119 GGGTCCC [GA]G^G[AT]CC[CT] 191 GGGACCC SexAI 4294 ACCTGGT A^CC[AT]GGT 5395 ACCAGGT SmaI 42 CCCGGG CCC^GGG 1732 CCCGGG SpeI 744 ACTAGT A^CTAGT 4543 ACTAGT SphI 1009 GCATGC GCATG^C 2481 GCATGC SspI 2009 AATATT AAT^ATT 2871 AATATT Tth111I 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC 4293 GACCTGGTC Van91I 649 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3244 CCAAAGATTGG XmaCI 42 CCCGGG C^CCGGG 1732 CCCGGG XmaI 42 CCCGGG C^CCGGG 1732 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 131 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AflIII AluI Alw44I AlwI AlwNI AocI ApaLI ApoI AspHI AvaI AvaII BanI BanII BbeI BbsI BbvCI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI BstXI BstYI Bsu36I Cac8I CfoI Cfr13I DdeI DpnII DraI DraII DrdI EaeI EarI Eco81I EcoO109I EcoRII EcoRV EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HincII HindII HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MfeI MflI MnlI MseI MslI MspA1I MspI MunI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PaeR7I PleI PshAI PstI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI SmlI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XbaI XhoI XhoII