##################################
# NO CUT ENZYMES ( 53 enzymes) #
##################################
AatII AccIII AclI AgeI AhdI
Aor51HI AscI AspEI BcgI BclI
BglI BsaBI BsaWI BseAI BsiWI
Bsp1407I BspDI BspEI BsrGI BssHII
BstBI BstEII BstPI ClaI CpoI
DraIII Eam1105I Eco47III EcoO65I EcoRI
FbaI FseI MamI MluI MroI
NruI NspV PacI PinAI PmeI
Psp1406I PvuI RsrII SacI SalI
SbfI SfiI SfuI SgrAI SnaBI
Sse8387I SspBI SwaI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
Acc65I 1728 GGTACC G^GTACC
AflII 5027 CTTAAG C^TTAAG
ApaI 344 GGGCCC GGGCC^C
AseI 5162 ATTAAT AT^TAAT
AsnI 5162 ATTAAT AT^TAAT
Asp700I 2007 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1728 GGTACC G^GTACC
BbrPI 3012 CACGTG CAC^GTG
BfrI 5027 CTTAAG C^TTAAG
BglII 5221 AGATCT A^GATCT
BsaAI 3012 CACGTG [CT]AC^GT[GA]
BsaI 5861 GGTCTC GGTCTC(1/5)
BsgI 5754 CTGCAC GTGCAG(16/14)
BsiEI 142 CGGCCG CG[GA][CT]^CG
BspHI 2495 TCATGA T^CATGA
BssSI 3852 CTCGTG CACGAG(-5/-1)
Bst1107I 4190 GTATAC GTA^TAC
BstZ17I 4190 GTATAC GTA^TAC
EagI 142 CGGCCG C^GGCCG
EclXI 142 CGGCCG C^GGCCG
Eco52I 142 CGGCCG C^GGCCG
EcoNI 215 CCTTGCGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1011 ATGCAT ATGCA^T
HindIII 3432 AAGCTT A^AGCTT
KpnI 1728 GGTACC GGTAC^C
KspI 152 CCGCGG CCGC^GG
NcoI 910 CCATGG C^CATGG
NdeI 653 CATATG CA^TATG
NheI 99 GCTAGC G^CTAGC
NotI 141 GCGGCCGC GC^GGCCGC
NsiI 1011 ATGCAT ATGCA^T
PmaCI 3012 CACGTG CAC^GTG
PmlI 3012 CACGTG CAC^GTG
PshBI 5162 ATTAAT AT^TAAT
RcaI 2495 TCATGA T^CATGA
SacII 152 CCGCGG CCGC^GG
ScaI 1789 AGTACT AGT^ACT
XcmI 4221 CCACTTTGTCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2007 GAAATATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccI 4190 GTATAC GT^[AC][GT]AC
5941 GTCTAC
AspI 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACCTGGTC
AviII 218 TGCGCA TGC^GCA
5629 TGCGCA
AvrII 5079 CCTAGG C^CTAGG
5497 CCTAGG
BalI 4213 TGGCCA TGG^CCA
5701 TGGCCA
BamHI 2105 GGATCC G^GATCC
5555 GGATCC
BciVI 2859 GGATAC GTATCC(6/5)
5883 GTATCC
BlnI 5079 CCTAGG C^CTAGG
5497 CCTAGG
BlpI 2513 GCTTAGC GC^T[ACGT]AGC
5811 GCTGAGC
Bpu1102I 2513 GCTTAGC GC^T[ACGT]AGC
5811 GCTGAGC
BspLU11I 3712 ACATGT A^CATGT
4987 ACATGT
BsrBI 16 CCGCTC CCGCTC(-3/-3)
115 GAGCGG
BsrDI 1043 CATTGC GCAATG(2/0)
2506 CATTGC
BsrFI 252 GCCGGC [GA]^CCGG[CT]
1940 GCCGGC
CelII 2513 GCTTAGC GC^T[ACGT]AGC
5811 GCTGAGC
Cfr10I 252 GCCGGC [GA]^CCGG[CT]
1940 GCCGGC
DsaI 152 CCGCGG C^C[GA][CT]GG
910 CCATGG
FspI 218 TGCGCA TGC^GCA
5629 TGCGCA
HpaI 3454 GTTAAC GTT^AAC
4558 GTTAAC
MluNI 4213 TGGCCA TGG^CCA
5701 TGGCCA
MscI 4213 TGGCCA TGG^CCA
5701 TGGCCA
NaeI 252 GCCGGC GCC^GGC
1940 GCCGGC
NgoMIV 252 GCCGGC G^CCGGC
1940 GCCGGC
PflFI 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACCTGGTC
PflMI 649 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3244 CCAAAGATTGG
PpuMI 119 GGGTCCC [GA]G^G[AT]CC[CT]
191 GGGACCC
SexAI 4294 ACCTGGT A^CC[AT]GGT
5395 ACCAGGT
SmaI 42 CCCGGG CCC^GGG
1732 CCCGGG
SpeI 744 ACTAGT A^CTAGT
4543 ACTAGT
SphI 1009 GCATGC GCATG^C
2481 GCATGC
SspI 2009 AATATT AAT^ATT
2871 AATATT
Tth111I 3058 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
4293 GACCTGGTC
Van91I 649 CCACCATATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3244 CCAAAGATTGG
XmaCI 42 CCCGGG C^CCGGG
1732 CCCGGG
XmaI 42 CCCGGG C^CCGGG
1732 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 131 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AflIII AluI Alw44I AlwI AlwNI
AocI ApaLI ApoI AspHI AvaI
AvaII BanI BanII BbeI BbsI
BbvCI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaHI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmBI
BsmFI BsmI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstXI BstYI Bsu36I Cac8I
CfoI Cfr13I DdeI DpnII DraI
DraII DrdI EaeI EarI Eco81I
EcoO109I EcoRII EcoRV EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeII MaeIII
MboI MboII MfeI MflI MnlI
MseI MslI MspA1I MspI MunI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV NspI PaeR7I
PleI PshAI PstI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SfoI SmlI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XbaI XhoI
XhoII