##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI Alw44I ApaLI AscI
Asp718I AspI AvaI AvrII BalI
BamHI BbsI BbvCI BcgI BciVI
BfrI BlnI BpuAI BsaWI BseAI
BsiWI BsoBI BspEI BspHI BsrFI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstUI BstZ17I Cfr10I CpoI
EagI EclXI Eco52I EcoNI EcoO65I
EcoRV FseI HpaI KpnI KspI
MluI MluNI MroI MscI MvnI
NaeI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI Psp1406I PvuI
RcaI RsrII SacI SacII SalI
SbfI SfiI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I StuI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
ApaI 986 GGGCCC GGGCC^C
AseI 3497 ATTAAT AT^TAAT
AsnI 3497 ATTAAT AT^TAAT
Asp700I 4413 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 9 TGCGCA TGC^GCA
BbeI 357 GGCGCC GGCGC^C
BclI 2737 TGATCA T^GATCA
BglI 979 GCCCAATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 952 AGATCT A^GATCT
BsaBI 728 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1648 GGTCTC GGTCTC(1/5)
BsiEI 1029 CGACCG CG[GA][CT]^CG
BspDI 236 ATCGAT AT^CGAT
BstXI 762 CCATGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
ClaI 236 ATCGAT AT^CGAT
DraII 1441 GGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 402 TGGCCG [CT]^GGCC[GA]
EcoO109I 1441 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 3472 GAATTC G^AATTC
EcoT22I 264 ATGCAT ATGCA^T
FbaI 2737 TGATCA T^GATCA
FspI 9 TGCGCA TGC^GCA
KasI 357 GGCGCC G^GCGCC
MamI 728 GATCCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NarI 357 GGCGCC GG^CGCC
NsiI 264 ATGCAT ATGCA^T
PflMI 859 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1441 GGGACCT [GA]G^G[AT]CC[CT]
PshBI 3497 ATTAAT AT^TAAT
SapI 1843 GAAGAGC GCTCTTC(1/4)
SexAI 1629 ACCAGGT A^CC[AT]GGT
SfoI 357 GGCGCC GGC^GCC
SmlI 3048 CTTGAG C^T[CT][GA]AG
SspI 4136 AATATT AAT^ATT
SwaI 4139 ATTTAAAT ATTT^AAAT
Van91I 859 CCACACGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1408 TCTAGA T^CTAGA
XmnI 4413 GAACAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AatII 1102 GACGTC GACGT^C
4167 GACGTC
AhdI 1927 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACTCCAGGTC
AocI 1242 CCTTAGG CC^T[ACGT]AGG
1878 CCTGAGG
Aor51HI 463 AGCGCT AGC^GCT
555 AGCGCT
AspEI 1927 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACTCCAGGTC
BanI 357 GGCGCC G^G[CT][GA]CC
1129 GGTGCC
BanII 986 GGGCCC G[GA]GC[CT]^C
1759 GGGCTC
BbrPI 491 CACGTG CAC^GTG
3098 CACGTG
BlpI 558 GCTCAGC GC^T[ACGT]AGC
1851 GCTCAGC
Bpu1102I 558 GCTCAGC GC^T[ACGT]AGC
1851 GCTCAGC
BsmBI 472 CGTCTC CGTCTC(1/5)
48 GAGACG
BsrDI 3155 CATTGC GCAATG(2/0)
3267 GCAATG
Bsu36I 1242 CCTTAGG CC^T[ACGT]AGG
1878 CCTGAGG
CelII 558 GCTCAGC GC^T[ACGT]AGC
1851 GCTCAGC
DraIII 3585 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
3753 CACCTTGTG
DrdI 913 GACTATCAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2554 GACAGAGCAGTC
Eam1105I 1927 GACTTACTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2593 GACTCCAGGTC
Eco47III 463 AGCGCT AGC^GCT
555 AGCGCT
Eco81I 1242 CCTTAGG CC^T[ACGT]AGG
1878 CCTGAGG
HincII 1309 GTCAAC GT[CT]^[GA]AC
4471 GTCAAC
HindII 1309 GTCAAC GT[CT]^[GA]AC
4471 GTCAAC
HindIII 2535 AAGCTT A^AGCTT
3312 AAGCTT
MfeI 2762 CAATTG C^AATTG
2936 CAATTG
MunI 2762 CAATTG C^AATTG
2936 CAATTG
NcoI 322 CCATGG C^CATGG
2961 CCATGG
PmaCI 491 CACGTG CAC^GTG
3098 CACGTG
PmlI 491 CACGTG CAC^GTG
3098 CACGTG
ScaI 1914 AGTACT AGT^ACT
4397 AGTACT
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AcsI AcyI AfaI AflIII
AluI AlwI AlwNI ApoI AspHI
AvaII BbvI BcnI BfaI BmyI
BpmI BsaAI BsaHI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsmI Bsp1286I Bsp1407I BspLU11I
BspMI BsrBI BsrGI BsrI BssKI
BstAPI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraI
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SspBI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII