##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AatII Acc65I AccIII AclI AgeI
Aor51HI AscI AseI AsnI Asp718I
AspI AviII BamHI BcgI BciVI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BspDI BspEI BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DrdI EagI EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HpaI KpnI
KspI MfeI MluI MroI MunI
NaeI NdeI NgoMIV NheI NotI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfuI SgrAI SmaI
SnaBI Sse8387I SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AccI 3854 GTAGAC GT^[AC][GT]AC
AhdI 4650 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 4886 GTGCAC G^TGCAC
ApaI 4146 GGGCCC GGGCC^C
ApaLI 4886 GTGCAC G^TGCAC
Asp700I 1382 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 4650 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 555 CCCGAG C^[CT]CG[GA]G
AvrII 2573 CCTAGG C^CTAGG
BbrPI 3514 CACGTG CAC^GTG
BcnI 207 CCGGG CC^[GC]GG
BglI 3493 GCCTCTTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 4554 AGATCT A^GATCT
BlnI 2573 CCTAGG C^CTAGG
BlpI 222 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 222 GCTCAGC GC^T[ACGT]AGC
BsaAI 3514 CACGTG [CT]AC^GT[GA]
BsaBI 4109 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsoBI 555 CCCGAG C^[CT]CG[GA]G
Bsp1407I 587 TGTACA T^GTACA
BspHI 2472 TCATGA T^CATGA
BsrFI 26 GCCGGT [GA]^CCGG[CT]
BsrGI 587 TGTACA T^GTACA
BssSI 2632 CACGAG CACGAG(-5/-1)
CelII 222 GCTCAGC GC^T[ACGT]AGC
Cfr10I 26 GCCGGT [GA]^CCGG[CT]
DraI 1832 TTTAAA TTT^AAA
Eam1105I 4650 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HgaI 1661 GCGTC GACGC(5/10)
HincII 341 GTTGAC GT[CT]^[GA]AC
HindII 341 GTTGAC GT[CT]^[GA]AC
MamI 4109 GATCTGTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NciI 207 CCGGG CC^[GC]GG
NruI 935 TCGCGA TCG^CGA
PflMI 4289 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 3514 CACGTG CAC^GTG
PmlI 3514 CACGTG CAC^GTG
RcaI 2472 TCATGA T^CATGA
ScaI 1838 AGTACT AGT^ACT
SpeI 1710 ACTAGT A^CTAGT
SphI 4808 GCATGC GCATG^C
SspBI 587 TGTACA T^GTACA
StuI 4863 AGGCCT AGG^CCT
Van91I 4289 CCAAGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 3438 CCAGGGATGGTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 1382 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 3123 AAATTC [GA]^AATT[CT]
5058 AAATTT
AcyI 5 GGCGCC G[GA]^CG[CT]C
23 GGCGCC
AflIII 1018 ACATGT A^C[GA][CT]GT
3659 ACATGT
AocI 4327 CCTGAGG CC^T[ACGT]AGG
4774 CCTGAGG
ApoI 3123 AAATTC [GA]^AATT[CT]
5058 AAATTT
BbeI 5 GGCGCC GGCGC^C
23 GGCGCC
BbsI 2146 GTCTTC GAAGAC(2/6)
4530 GTCTTC
BclI 892 TGATCA T^GATCA
4196 TGATCA
BpuAI 2146 GTCTTC GAAGAC(2/6)
4530 GTCTTC
BsaHI 5 GGCGCC G[GA]^CG[CT]C
23 GGCGCC
BspLU11I 1018 ACATGT A^CATGT
3659 ACATGT
BsrBI 1247 CCGCTC CCGCTC(-3/-3)
657 GAGCGG
BstXI 3300 CCACCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
4165 CCACAGAAGTGG
Bsu36I 4327 CCTGAGG CC^T[ACGT]AGG
4774 CCTGAGG
DraIII 2438 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
3371 CACTCTGTG
DsaI 1345 CCATGG C^C[GA][CT]GG
4757 CCATGG
Eco81I 4327 CCTGAGG CC^T[ACGT]AGG
4774 CCTGAGG
FbaI 892 TGATCA T^GATCA
4196 TGATCA
Hin1I 5 GGCGCC G[GA]^CG[CT]C
23 GGCGCC
HindIII 1555 AAGCTT A^AGCTT
1982 AAGCTT
KasI 5 GGCGCC G^GCGCC
23 GGCGCC
MaeII 3515 ACGT A^CGT
4160 ACGT
NarI 5 GGCGCC GG^CGCC
23 GGCGCC
NcoI 1345 CCATGG C^CATGG
4757 CCATGG
PpuMI 2652 GGGTCCC [GA]G^G[AT]CC[CT]
4377 AGGTCCT
PshAI 3944 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
4623 GACCTTAGTC
SapI 4795 GAAGAGC GCTCTTC(1/4)
683 GCTCTTC
SfoI 5 GGCGCC GGC^GCC
23 GGCGCC
SspI 1826 AATATT AAT^ATT
5045 AATATT
XbaI 2690 TCTAGA T^CTAGA
4305 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AflII AluI
AlwI AlwNI AspHI AvaII BalI
BanI BanII BbvCI BbvI BfaI
BfrI BmyI BpmI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrDI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MluNI MnlI MscI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII